KEGG   ENZYME: 6.4.1.6
Entry
EC 6.4.1.6                  Enzyme                                 
Name
acetone carboxylase
Class
Ligases;
Forming carbon-carbon bonds;
Ligases that form carbon-carbon bonds (only sub-subclass identified to date)
Sysname
acetone:carbon-dioxide ligase (AMP-forming)
Reaction(IUBMB)
acetone + hydrogen carbonate + 2 ATP + 3 H2O = acetoacetate + 2 AMP + 4 phosphate [RN:R05735]
Reaction(KEGG)
R05735
Substrate
acetone [CPD:C00207];
hydrogen carbonate [CPD:C00288];
ATP [CPD:C00002];
H2O [CPD:C00001]
Product
acetoacetate [CPD:C00164];
AMP [CPD:C00020];
phosphate [CPD:C00009]
Comment
Requires Mg2+ and ATP. The reaction involves separate phosphorylation of hydrogencarbonate and acetone, forming carboxyphosphate and phosphoenolacetone, respectively, which are combined to form the final product. The enzyme from Xanthobacter sp. strain Py2 also carboxylates butan-2-one to 3-oxopentanoate.
History
EC 6.4.1.6 created 2001
Orthology
K10854  acetone carboxylase, alpha subunit
K10855  acetone carboxylase, beta subunit
K10856  acetone carboxylase, gamma subunit
Genes
GHO: AL542_03630 AL542_03635 AL542_03640
AVN: Avin_20910(acxA) Avin_20920(acxB) Avin_20930(acxC)
AVL: AvCA_20910(acxA) AvCA_20920(acxB) AvCA_20930(acxC)
AVD: AvCA6_20910(acxA) AvCA6_20920(acxB) AvCA6_20930(acxC)
ACX: Achr_24430(acxC) Achr_24440(acxB) Achr_24450(acxA)
PPIS: B1L02_05720 B1L02_05725 B1L02_05730
AEH: Mlg_1029 Mlg_1030 Mlg_1031
SDF: ACG33_03915 ACG33_03920 ACG33_03925
CHRO: CXB49_13600 CXB49_13605 CXB49_13610
AQS: DKK66_16610 DKK66_16615 DKK66_16620
CNC: CNE_2c13060(acxA) CNE_2c13070(acxB) CNE_2c13080(acxC)
CUH: BJN34_28755 BJN34_28760 BJN34_28765
REU: Reut_C6172 Reut_C6173 Reut_C6174
RME: Rmet_4105(acxA) Rmet_4106(acxB) Rmet_4107(acxC)
CBW: RR42_s1691 RR42_s1692 RR42_s1693
CCUP: BKK81_09935 BKK81_09940 BKK81_09945
CUP: BKK80_10375 BKK80_10380 BKK80_10385
CUU: BKK79_02725 BKK79_02730 BKK79_02735
COX: E0W60_18765 E0W60_18770 E0W60_18775
CUK: KB879_11590 KB879_11595 KB879_11600
BOK: DM82_4513(acxC) DM82_4514(acxB) DM82_4515(acxA)
BOC: BG90_6078 BG90_6079 BG90_6080(acxC)
BUU: WS70_19475 WS70_19480 WS70_19485
BANN: JFN94_28000 JFN94_28005 JFN94_28010
BURK: DM992_21385 DM992_21390 DM992_21395
BPH: Bphy_6150 Bphy_6151 Bphy_6152
TVL: FAZ95_31535 FAZ95_31540 FAZ95_31545
AKA: TKWG_04145 TKWG_04150
AMIM: MIM_c06390(acxA) MIM_c06400(acxB) MIM_c06410(acxC)
NARM: N7E01_08320
PNA: Pnap_0941 Pnap_0942 Pnap_0943
ADN: Alide_1502 Alide_1503 Alide_1504
ADK: Alide2_3422 Alide2_3423 Alide2_3424
HTN: KI616_16680 KI616_16685 KI616_16690
IDC: LRM40_07745 LRM40_07750 LRM40_07755
METR: BSY238_3044 BSY238_3045 BSY238_3046
EBA: ebA4700(acxA) ebA4701(acxB) ebA4702(acxC)
ABRE: pbN1_07560(acxC) pbN1_07570(acxB) pbN1_07580(acxA)
APET: ToN1_19230(acxC) ToN1_19240(acxB) ToN1_19250(acxA)
DAR: Daro_1018 Daro_1019 Daro_1020
DDEN: KI615_08160 KI615_08165 KI615_08170
AZA: AZKH_0561(acxA) AZKH_0562(acxB) AZKH_0563(acxC)
AZI: AzCIB_4004 AzCIB_4005 AzCIB_4006
AZD: CDA09_19355 CDA09_19360 CDA09_19365
TMZ: Tmz1t_3469 Tmz1t_3470 Tmz1t_3471
THK: CCZ27_01695 CCZ27_01700 CCZ27_01705
AZQ: G3580_00140 G3580_00145 G3580_00150
ACOM: CEW83_18660 CEW83_18665 CEW83_18670
MLO: mlr5245 mlr5246 mlr5247
MLN: A9174_01300 A9174_01305 A9174_01310
MCI: Mesci_0253 Mesci_0254 Mesci_0255
MCIC: A4R28_27095 A4R28_27100 A4R28_27105
MOP: Mesop_2073 Mesop_2074 Mesop_2075
MAMO: A6B35_06455 A6B35_06460 A6B35_06465
MESW: A9K65_001265 A9K65_001270 A9K65_001275
MHUA: MCHK_6295 MCHK_6296 MCHK_6297
MJR: EB229_01325 EB229_01330 EB229_01335
MERD: EB233_01400 EB233_01405 EB233_01410
KMN: HW532_13065 HW532_13070 HW532_13075
SME: SM_b20406(hyuA) SM_b20407(hyuB) SM_b20408
SMK: Sinme_3770 Sinme_3771 Sinme_3772
SMQ: SinmeB_4266 SinmeB_4267 SinmeB_4268
SMI: BN406_06264 BN406_06265 BN406_06266(acxA)
SMEL: SM2011_b20406(hyuA) SM2011_b20407(hyuB) SM2011_b20408
SMER: DU99_31260 DU99_31265 DU99_31270
SMD: Smed_3715 Smed_3716 Smed_3717
SKM: PZL22_001170 PZL22_001171 PZL22_001172
RPC: RPC_3603 RPC_3604 RPC_3605
XAU: Xaut_3509 Xaut_3510 Xaut_3511
XDI: EZH22_17690 EZH22_17695 EZH22_17700
AZC: AZC_2921 AZC_2922 AZC_2923
LNE: FZC33_11855 FZC33_11860 FZC33_11865
APOL: K9D25_22125 K9D25_22130 K9D25_22135
MCH: Mchl_5521 Mchl_5522 Mchl_5523
HMC: HYPMC_0473(acxC) HYPMC_0474(acxB) HYPMC_0475(acxA)
RVA: Rvan_3476 Rvan_3477 Rvan_3478
RLAC: QMO75_11830 QMO75_11835 QMO75_11840
MMED: Mame_04504(acxA) Mame_04505(acxB) Mame_04506(acxC)
CID: P73_2858 P73_2859 P73_2860
CON: TQ29_15905 TQ29_15910 TQ29_15915
CAZT: LV780_20910 LV780_20915 LV780_20920
RCP: RCAP_rcc01336(acxA) RCAP_rcc01337(acxB) RCAP_rcc01338(acxC)
PDE: Pden_4261 Pden_4262 Pden_4263
PYE: A6J80_00050 A6J80_00055 A6J80_00060
PKD: F8A10_17600 F8A10_17605 F8A10_17610
PPAN: ESD82_13605 ESD82_13610 ESD82_13615
PMEH: JWJ88_20050 JWJ88_20055 JWJ88_20060
PVER: E3U25_15255 E3U25_15260 E3U25_15265
RSU: NHU_02461 NHU_02462 NHU_02463
RHM: B5V46_07325 B5V46_07330 B5V46_07335
RHC: RGUI_4268 RGUI_4269 RGUI_4270
RAP: RHOA_4205(acxA) RHOA_4206(acxB) RHOA_4207(acxC)
TMO: TMO_1428 TMO_1429 TMO_1430(hyuA)
HPY: HP_0695 HP_0696 HP_0697
HEO: C694_03590 C694_03595 C694_03600
HPJ: jhp_0631 jhp_0632 jhp_0633
HPA: HPAG1_0680 HPAG1_0681 HPAG1_0682
HPS: HPSH_03365 HPSH_03370 HPSH_03375
HHP: HPSH112_03530 HPSH112_03535 HPSH112_03540
HHQ: HPSH169_03575 HPSH169_03580 HPSH169_03585
HHR: HPSH417_03340 HPSH417_03345 HPSH417_03350
HPG: HPG27_652 HPG27_653
HPP: HPP12_0707 HPP12_0708
HPB: HELPY_0673 HELPY_0674
HPL: HPB8_896(hyuA3) HPB8_897(acxB) HPB8_898(acxC)
HPC: HPPC_03295 HPPC_03300 HPPC_03305
HCA: HPPC18_03375 HPPC18_03380 HPPC18_03385
HPM: HPSJM_03525 HPSJM_03530 HPSJM_03535
HPE: HPELS_03065 HPELS_03070 HPELS_03075
HPO: HMPREF4655_20905 HMPREF4655_20906 HMPREF4655_20907
HPI: hp908_0699 hp908_0700
HPQ: hp2017_0677 hp2017_06781
HPW: hp2018_0678 hp2018_06791
HPU: HPCU_03745 HPCU_03750 HPCU_03755
HEF: HPF16_0707 HPF16_0708 HPF16_0709
HPF: HPF30_0635 HPF30_0636 HPF30_0637
HEQ: HPF32_0627 HPF32_0628 HPF32_0629
HEX: HPF57_0720 HPF57_0721 HPF57_0722
HPT: HPSAT_03320 HPSAT_03325 HPSAT_03330
HPZ: HPKB_0650 HPKB_0651(hyuB) HPKB_0652
HPV: HPV225_0703 HPV225_0704 HPV225_0705
HPX: HMPREF0462_0758 HMPREF0462_0759 HMPREF0462_0760
HEN: HPSNT_03580 HPSNT_03585 HPSNT_03590
HPH: HPLT_03470 HPLT_03475 HPLT_03480
HEG: HPGAM_03565 HPGAM_03570 HPGAM_03575
HPN: HPIN_03160 HPIN_03165 HPIN_03170
HEP: HPPN120_03230 HPPN120_03235 HPPN120_03240
HEU: HPPN135_03425 HPPN135_03430 HPPN135_03435
HES: HPSA_03295 HPSA_03300 HPSA_03305
HPYS: HPSA20_0727 HPSA20_0728 HPSA20_0729
HCN: HPB14_03205 HPB14_03210 HPB14_03215
HPD: KHP_0625 KHP_0626 KHP_0627(hyuA)
HEY: MWE_0807 MWE_0808 MWE_0809
HER: C695_03590 C695_03600 C695_03605
HEI: C730_03595 C730_03605 C730_03610
HPYA: HPAKL117_03290 HPAKL117_03295 HPAKL117_03300
HPYK: HPAKL86_03670 HPAKL86_03675
HPYO: HPOK113_0707 HPOK113_0708 HPOK113_0709
HPYL: HPOK310_0685 HPOK310_0686 HPOK310_0687
HPYB: HPOKI102_03545 HPOKI102_03550
HPYC: HPOKI112_03555 HPOKI112_03560 HPOKI112_03565
HPYD: HPOKI128_03310 HPOKI128_03315 HPOKI128_03320
HPYE: HPOKI154_03540 HPOKI154_03545 HPOKI154_03550
HPYF: HPOKI422_03545 HPOKI422_03550 HPOKI422_03555
HPYG: HPOKI673_03515 HPOKI673_03520 HPOKI673_03525
HPYH: HPOKI828_03535 HPOKI828_03540 HPOKI828_03545
HPYJ: HPOKI898_03550 HPOKI898_03555 HPOKI898_03560
HPYR: K747_07580 K747_07585 K747_13225
HPYI: K750_05755 K750_05760 K750_05765
HPYU: K751_03890 K751_03895 K751_03900
HPYM: K749_02195 K749_02200 K749_02205
HEM: K748_00665 K748_00670 K748_00675
HEB: U063_0999 U063_1000 U063_1001
HEZ: U064_1003 U064_1004 U064_1005
HAC: Hac_0949 Hac_0950(hyuB) Hac_0951(hyuA)
BMET: BMMGA3_02520(acxA) BMMGA3_02525(acxB) BMMGA3_02530(acxC)
BFD: NCTC4823_02247(acxC) NCTC4823_02248(acxB_4) NCTC4823_02249(acxA_4)
BACI: B1NLA3E_12270 B1NLA3E_12275 B1NLA3E_12280
BAQU: K6959_06485 K6959_06490 K6959_06495
OJD: NP439_21175 NP439_21180 NP439_21185
GWC: GWCH70_1201
GMC: GY4MC1_2265 GY4MC1_2266 GY4MC1_2267 GY4MC1_2586 GY4MC1_2587 GY4MC1_2588
GTH: Geoth_2352 Geoth_2353 Geoth_2354 Geoth_2606 Geoth_2607 Geoth_2608
PTL: AOT13_09530 AOT13_09535 AOT13_09540 AOT13_11005 AOT13_11010 AOT13_11015
PTB: DER53_10555 DER53_10560 DER53_10565
PARG: PspKH34_25260 PspKH34_25270 PspKH34_25280
LAO: AOX59_03500 AOX59_03505 AOX59_03510
LEX: Len3610_12060 Len3610_12065 Len3610_12070
APAK: AP3564_08145 AP3564_08150 AP3564_08155
ACOP: RI196_03160 RI196_03165 RI196_03170
RUE: DT065_12355 DT065_12360 DT065_12365
SCIA: HUG15_09350 HUG15_09355 HUG15_09360
TEI: QS257_12885
HYI: K2M58_07205 K2M58_07210 K2M58_07215
BTS: Btus_1119 Btus_1120 Btus_1121 Btus_1124 Btus_1125 Btus_1126 Btus_2467 Btus_2468 Btus_2469
KYR: CVV65_05895 CVV65_05900 CVV65_05905 CVV65_10520 CVV65_10525 CVV65_10545 CVV65_10560 CVV65_10565 CVV65_10570 CVV65_12340 CVV65_12345 CVV65_12350
EFF: skT53_16340 skT53_16350 skT53_16360
PABS: JIR001_05790 JIR001_05800 JIR001_05810
THEF: E1B22_00250 E1B22_00255 E1B22_00260
CFN: CFAL_06320 CFAL_06325 CFAL_06330
SAIU: J4H86_13870 J4H86_13875 J4H86_13880
SVI: Svir_21470
RRS: RoseRS_2091 RoseRS_2092 RoseRS_2093
RCA: Rcas_1776 Rcas_1777 Rcas_1778
HDO: MUK72_17495 MUK72_17500 MUK72_17505 MUK72_17520
NHO: HWV23_00160 HWV23_00165 HWV23_00170
NARA: QQ977_04620 QQ977_04625 QQ977_04630 QQ977_04635
NBG: DV706_18735 DV706_18740
NAS: GCU68_16825 GCU68_16850 GCU68_16855 GCU68_16860
SCAS: SACC_14250 SACC_14260 SACC_14270
SULL: EWF20_01240 EWF20_01245 EWF20_01250
 » show all
Reference
1  [PMID:9237998]
  Authors
Sluis MK, Ensign SA.
  Title
Purification and characterization of acetone carboxylase from Xanthobacter strain Py2.
  Journal
Proc Natl Acad Sci U S A 94:8456-61 (1997)
DOI:10.1073/pnas.94.16.8456
  Sequence
[xau:Xaut_3509 Xaut_3510 Xaut_3511]
Reference
2  [PMID:22020645]
  Authors
Schuhle K, Heider J.
  Title
Acetone and butanone metabolism of the denitrifying bacterium "Aromatoleum aromaticum" demonstrates novel biochemical properties of an ATP-dependent aliphatic ketone carboxylase.
  Journal
J Bacteriol 194:131-41 (2012)
DOI:10.1128/JB.05895-11
Other DBs
ExplorEnz - The Enzyme Database: 6.4.1.6
IUBMB Enzyme Nomenclature: 6.4.1.6
ExPASy - ENZYME nomenclature database: 6.4.1.6
BRENDA, the Enzyme Database: 6.4.1.6
CAS: 189258-15-9
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