Entry
Name
sterol 24-C-methyltransferase;
Delta24-methyltransferase;
Delta24-sterol methyltransferase;
zymosterol-24-methyltransferase;
S-adenosyl-4-methionine:sterol Delta24-methyltransferase;
SMT1;
24-sterol C-methyltransferase;
S-adenosyl-L-methionine:Delta24(23)-sterol methyltransferase;
phytosterol methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:zymosterol 24-C-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol [RN:
R04427 ]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:
C00019 ];
5alpha-cholesta-8,24-dien-3beta-ol [CPD:
C05437 ]
Product
S-adenosyl-L-homocysteine [CPD:
C00021 ];
24-methylene-5alpha-cholest-8-en-3beta-ol [CPD:
C04525 ]
Comment
Requires glutathione. Acts on a range of sterols with a 24(25)-double bond in the sidechain. While zymosterol is the preferred substrate it also acts on desmosterol, 5alpha-cholesta-7,24-dien-3beta-ol, 5alpha-cholesta-5,7,24-trien-3beta-ol, 4alpha-methylzymosterol and others. S-Adenosyl-L-methionine attacks the Si-face of the 24(25) double bond and the C-24 hydrogen is transferred to C-25 on the Re face of the double bond.
History
EC 2.1.1.41 created 1972, modified 2001
Pathway
ec01110 Biosynthesis of secondary metabolites
Orthology
K00559 sterol 24-C-methyltransferase
Genes
BMY : BM_BM4647(Bma-strm-1)
CSAT : 104705624 104735382 104769590
BNA : 106371823 106431054 106440921
THJ : 104809850 104819084 104819086 104819087
MINC : 123197557 123214703 123216668 123216669 123216670
TCC : 18598956 18614599 18614600
HSYR : 120129642 120136785 120165276 120188812
EGR : 104454605 104454641 104454642 104455966
GMX : 100796978 100817534 100818061 548073
GSJ : 114372208 114378245 114379538 114386323
PVU : 137834769 137836171 137836176
CCAJ : 109799147 109806010
APRC : 113854972 113865316
AHF : 112716746 112738324 112783834 112790500
LANG : 109333713 109346157
PCIN : 129296047 129315800
QSA : O6P43_006507 O6P43_029576
MDM : 103418631 103438422 114823791
MSYL : 126613669 126628384
MCHA : 111020326 111020366
CMAX : 111481504 111481505 111493094
CMOS : 111441927 111442447 111452027
CPEP : 111787513 111789805 111789806
MESC : 110618568 110620358 110623913
POP : 18095100 18106161 7467341
PEU : 105117376 105126337 105131854 105141731
PALZ : 118027720 118047027 118051651
PNZ : 133677017 133680239 133689251
JRE : 108987693 108988702 109019397
CILL : 122292529 122292668 122293402
CAVE : 132167241 132173586
QLO : 115953357 115967771 115986962
VRI : 117919779 117928779 117928780
SPEN : 107008176 107032350
SSTN : 125853521 125866069
CANN : 107839483 107845155
NTA : 107772103 107806298 107813194 107813488 107821929 107825894
NSY : 104213731 104221372 104248706
NTO : 104104807 104117345 104120777
NAU : 109216067 109225324 109237978
SSPL : 121759270 121791175 121805340 121809614
SHIS : 125194076 125217903
HAN : 110888576 110918161 110921577
LSV : 111900335 111912645 111912658
CSIN : 114279643 114312087 114318153
RVL : 131326980 131327876 131334351 131334352 131334353
AEW : 130756790 130780608 130782335
MING : 122071445 122071905 122091901
TSS : 122642777 122642790 122642791
PSOM : 113313126 113322869 113354420
OSA : 4342690(Smt1-1) 9269350
OGL : 127755018 127766216 127779788
TAES : 123093722 123105904 543246
LPER : 127292258 127323344
LRD : 124674874 124674972 124697778 124697779
SBI : 8057107 8064726 8078421 8081208
ZMA : 100282501 100286381 541682
MFLO : 136486773 136513377 136517854 136535197 136545482 136549562 136549565
SITA : 101753762 101756008 101780888
SVS : 117834191 117845345 117866533
PVIR : 120639714 120644214 120644215 120644245 120645629 120649372 120659670 120665784
PHAI : 112872433 112877095 112883041
PDA : 103696988 103696989 103697536 103717656 103717657 113461272 120104527
EGU : 105035517 105046511 105055167
MUS : 103979889 103980161 103993068 135609970
ZOF : 122020552 122020864 122024007 122024078 122024744 122051188 122055892
DCT : 110109799 110110369 110116065
MSIN : 131221626 131222583
ACAK : 137945411 137949864
ATR : 18428920 18428921 18428922
SMO : SELMODRAFT_449609(SMT1-2) SELMODRAFT_449613(SMT1-1)
GSL : Gasu_26310 Gasu_60150
NCS : NCAS_0H02570(NCAS0H02570)
NDI : NDAI_0C01050(NDAI0C01050)
TPF : TPHA_0D02350(TPHA0D02350)
TBL : TBLA_0D01630(TBLA0D01630)
TDL : TDEL_0A03930(TDEL0A03930)
KAF : KAFR_0C05410(KAFR0C05410)
KNG : KNAG_0B03670(KNAG0B03670)
LBG : 92208639(LODBEIA_P34430)
CAL : CAALFM_C302150CA(ERG6)
YLI : 2908368(YALI2_F00173g)
NCR : NCU02502 NCU03006(erg-4)
NTE : NEUTE1DRAFT150553(NEUTE1DRAFT_150553) NEUTE1DRAFT73978(NEUTE1DRAFT_73978)
SMP : 10808713(SMAC4_05969) 10810018(SMAC4_01214)
PAN : PODANSg3800 PODANSg4053 PODANSg4704
PBEL : QC761_115810(ERG6_1) QC761_124410(ERG6_2)
PPSD : QC762_115810(ERG6_1) QC762_124410(ERG6_2)
PPSP : QC763_115810(ERG6_1) QC763_124410(ERG6_2)
PPSA : QC764_115810(ERG6_1) QC764_124410(ERG6_2)
MTM : MYCTH_110670 MYCTH_42269
MGR : MGG_04346 MGG_10568 MGG_10860
PPEI : PpBr36_03960 PpBr36_10036
PGRI : PgNI_03580 PgNI_09085 PgNI_11040
TMN : UCRPA7_4321 UCRPA7_5376 UCRPA7_8731
SSCK : SPSK_06325 SPSK_06557 SPSK_06653
FGR : FGSG_02783 FGSG_05740
FPU : FPSE_05610 FPSE_08317
FPOA : FPOAC1_005133(ERG6_1) FPOAC1_008700(ERG4)
FVN : FVRRES_05661 FVRRES_09296
FVR : FVEG_05904 FVEG_13091
FOX : FOXG_08765 FOXG_15629
NHE : NECHADRAFT_101488 NECHADRAFT_33136 NECHADRAFT_59530
FFC : NCS54_00221300 NCS54_00445000
FKR : NCS57_00216400 NCS57_00451800
FMU : J7337_004853(ERG6_2) J7337_008919(ERG6_3)
TRE : TRIREDRAFT_121166 TRIREDRAFT_56236
TRR : M419DRAFT_108988 M419DRAFT_72858
TATV : 25776029(TrAtP1_002228) 25785708(TrAtP1_007734)
TASP : 36608857(ERG6_3) 36615740(ERG6_1)
MBRN : 26241702(erg6_0) 26243633(erg6_1)
PCHM : VFPPC_07182 VFPPC_10999
AMUS : LMH87_001661 LMH87_006959 LMH87_009165
PLJ : 28892488(PLICBS_006480) 28893235(PLICBS_006719)
PTKZ : JDV02_008053(ERG6_1) JDV02_009647(ERG6_2)
VAL : VDBG_01914 VDBG_08910
VDA : VDAG_01643 VDAG_06572
CFJ : CFIO01_00904 CFIO01_00910 CFIO01_12167
CLUP : CLUP02_08421 CLUP02_11674 CLUP02_11681
CHIG : CH63R_07675 CH63R_11163 CH63R_11195
CDET : 87944887(CDEST_08384) 87945928(CDEST_09425) 87945956(CDEST_09453)
SAPO : SAPIO_CDS4855 SAPIO_CDS6557
ELA : UCREL1_1004 UCREL1_1267 UCREL1_1354
PFY : PFICI_00782 PFICI_01643 PFICI_06847 PFICI_10966
SSL : SS1G_03467 SS1G_03686
BFU : BCIN_04g01200 BCIN_08g01540
PSCO : LY89DRAFT_628125 LY89DRAFT_684462 LY89DRAFT_725473 LY89DRAFT_789176
GLZ : GLAREA_04347 GLAREA_05164
ANI : ANIA_01614 ANIA_07146
AFM : AFUA_4G03630 AFUA_4G09190
ACT : ACLA_048560 ACLA_054780
NFI : NFIA_029790 NFIA_106980
AOR : AO090011000289 AO090012000932 AO090023000624
ANG : An04g04210 An14g01590 An16g00300
AFV : AFLA_002655 AFLA_006968 AFLA_007687
ALUC : AKAW2_40856S(ERG6) AKAW2_51426S
ACHE : ACHE_40345A ACHE_60580A(ERG6)
APUU : APUU_21581S APUU_22198S(ERG6_1) APUU_80754S(ERG6_2)
PCS : N7525_001128 N7525_007611
PDP : PDIP_13570 PDIP_13580 PDIP_57520 PDIP_76970
CIM : CIMG_06139 CIMG_08444 CIMG_09630
URE : UREG_02519 UREG_04245
PTRR : 6346862(PtrM4_146510)
AALT : CC77DRAFT_1024280 CC77DRAFT_984927
ZTR : MYCGRDRAFT_33363 MYCGRDRAFT_70113(ERG6) MYCGRDRAFT_70899 MYCGRDRAFT_98820
PFJ : MYCFIDRAFT_33221 MYCFIDRAFT_64115 MYCFIDRAFT_84237
FFU : CLAFUR5_00943 CLAFUR5_09835
CBET : CB0940_01475 CB0940_06157
BCOM : BAUCODRAFT_129875 BAUCODRAFT_37733 BAUCODRAFT_61348
NPA : UCRNP2_1782 UCRNP2_3336
CDEP : 91086185(L203_101973)
KMG : 30164895(I203_108188)
KNE : 92177636(IAR55_000376)
CCAC : CcaHIS019_0705320(ERG6)
ADL : AURDEDRAFT_110716 AURDEDRAFT_121438 AURDEDRAFT_139717
RSX : RhiXN_00557 RhiXN_05850
LBC : LACBIDRAFT_184995(erg6)
CCI : CC1G_05622 CC1G_06149 CC1G_07728
ABP : AGABI1DRAFT115299(AGABI1DRAFT_115299)
ABV : AGABI2DRAFT194260(AGABI2DRAFT_194260)
SCM : SCHCO_02052297(SCHCODRAFT_02052297) SCHCO_02512248(SCHCODRAFT_02512248) SCHCO_02601905(SCHCODRAFT_02601905) SCHCO_02627257(SCHCODRAFT_02627257) SCHCO_02680330(SCHCODRAFT_02680330) SCHCO_02751485(SCHCODRAFT_02751485)
PGR : PGTG_02203 PGTG_17346
PTRC : PtA15_2A427 PtA15_2A801 PtA15_2A804
PSTR : Pst134EA_005187 Pst134EA_005301
MLR : MELLADRAFT_41694 MELLADRAFT_73949
RIE : OCT59_023027 OCT59_026390
ACAN : ACA1_193300 ACA1_237870
TPS : THAPSDRAFT_32029 THAPSDRAFT_36203(SMT1)
AAF : AURANDRAFT_21939 AURANDRAFT_52760 AURANDRAFT_59110
TCR : 504191.10 505683.10 510185.10
LMA : LMJF_36_2380 LMJF_36_2390(LmSMT)
LIF : LINJ_36_2510 LINJ_36_2520
LDO : LDBPK_362510 LDBPK_362520
LMI : LMXM_36_2380 LMXM_36_2390
LMAT : 92510414(LSCM1_00250) 92510415(LSCM1_00251)
LOI : 92356276(LSCM4_00253) 92356277(LSCM4_00254)
LEIS : 94175909(GH5_00259) 94175910(GH5_00260)
LENR : 94167552(CUR178_00251)
LEIN : 94184027(JIQ42_00256) 94184028(JIQ42_00257)
PHET : 94286365(JKF63_00236)
NGR : NAEGRDRAFT_81379 NAEGRDRAFT_83028
» show all
Taxonomy
Reference
Authors
Moore JT, Gaylor JL.
Title
Isolation and purification of an S-adenosylmethionine: delta 24-sterol methyltransferase from yeast.
Journal
J Biol Chem 244:6334-40 (1969)
Reference
Authors
Venkatramesh M, Guo DA, Jia Z, Nes WD.
Title
Mechanism and structural requirements for transformation of substrates by the (S)-adenosyl-L-methionine:delta 24(25)-sterol methyl transferase from Saccharomyces cerevisiae.
Journal
Reference
3
Authors
Tong Y, McCourt BS, Guo D, Mangla AT, Zhou WX, Jenkins MD, Zhou W, Lopez M, Nes WD.
Title
, Stereochemical features of C-methylation on the path to Delta24(28)-methylene and Delta24(28)-ethylidene sterols: studies on the recombinant phytosterol methyl transferase from Arabidopsis thaliana.
Journal
Tetrahedron Lett 38:6115-6118 (1997)
Reference
Authors
Bouvier-Nave P, Husselstein T, Benveniste P
Title
Two families of sterol methyltransferases are involved in the first and the second methylation steps of plant sterol biosynthesis.
Journal
Sequence
Reference
Authors
Nes WD, McCourt BS, Zhou WX, Ma J, Marshall JA, Peek LA, Brennan M.
Title
Overexpression, purification, and stereochemical studies of the recombinant (S)-adenosyl-L-methionine: delta 24(25)- to delta 24(28)-sterol methyl transferase enzyme from Saccharomyces cerevisiae.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.41
ExPASy - ENZYME nomenclature database: 2.1.1.41
LinkDB
All DBs