Entry
Name
D-amino-acid transaminase;
D-aspartate transaminase;
D-alanine aminotransferase;
D-aspartic aminotransferase;
D-alanine-D-glutamate transaminase;
D-alanine transaminase;
D-amino acid aminotransferase
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
BRITE hierarchy
Sysname
D-alanine:2-oxoglutarate aminotransferase
Reaction(IUBMB)
D-alanine + 2-oxoglutarate = pyruvate + D-glutamate [RN:
R01148 ]
Reaction(KEGG)
Substrate
Product
Comment
A pyridoxal-phosphate protein. The enzyme from thermophilic Bacillus species acts on many D-amino acids with D-alanine and D-2-aminobutyrate as the best amino donors. It can similarly use any of several 2-oxo acids as amino acceptor, with 2-oxoglutarate and 2-oxobutyrate among the best. The enzyme from some other sources has a broader specificity [6].
History
EC 2.6.1.21 created 1972 (EC 2.6.1.10 created 1961, incorporated 1972), modified 2005
Pathway
ec00330 Arginine and proline metabolism
ec01120 Microbial metabolism in diverse environments
Orthology
Genes
GQU : AWC35_20870 AWC35_21325
PCAC : OI450_06230 OI450_12535
DDD : Dda3937_00800 Dda3937_02409(dat)
DZE : Dd1591_1294 Dd1591_2788
DDC : Dd586_1307 Dd586_2840
DZC : W909_06655 W909_13905
DSO : A4U42_02365 A4U42_16015
CED : LH89_05130 LH89_19295
DFN : CVE23_07620 CVE23_15250
DAQ : DAQ1742_01234(dat) DAQ1742_01746
DDA : Dd703_1218 Dd703_1465
LBQ : CKQ53_07465 CKQ53_17225
PHEI : NCTC12003_01607(dat)
THAP : FNC98_05425 FNC98_10710
TPSY : RGQ13_10260 RGQ13_14130
TNN : RI845_05410(dat) RI845_09395
TFT : RI844_03900 RI844_19660
LJR : NCTC11533_01439(dat)
LWA : SAMEA4504053_1742(dat)
LSS : NCTC12082_00586(dat)
SEDS : AAY24_00365 AAY24_14270
NCU : F0U83_01590 F0U83_02530
NJP : NEJAP_0312 NEJAP_0509
AJP : AMJAP_0423 AMJAP_2081
CHJ : NCTC10426_02367(dat)
BPL : BURPS1106A_0459(dat)
LMIR : NCTC12852_00632(dat)
BPER : BN118_0175 BN118_0315
BPET : B1917_0108 B1917_3054
BPEU : Q425_2190 Q425_33540
BPAR : BN117_0166 BN117_0327
BBH : BN112_3081 BN112_3244
BBM : BN115_0158 BN115_0319
BBX : BBS798_0166 BBS798_0328
BTRM : SAMEA390648701377(dat_1) SAMEA390648701530(dat_2)
AXY : AXYL_03859 AXYL_06268
AXX : ERS451415_06011(dat)
AIS : BUW96_08945 BUW96_24615
APES : FOC84_13870 FOC84_26175
AAEG : RA224_13680 RA224_31240
URU : DSM104443_04158(dat)
UPL : DSM104440_03681(dat)
THAG : CKCBHOJB_00143(dat)
NRH : T8J41_16045 T8J41_19930
AAK : AA2016_1294 AA2016_3772
AMIS : Amn_26780 Amn_50500
PHT : BLM14_08580 BLM14_26365
KMN : HW532_11150 HW532_16145
RBS : RHODOSMS8_00946(dat)
SMK : Sinme_1264 Sinme_5456
SMX : SM11_chr2105 SM11_pC1707
SMI : BN406_01195 BN406_05037
SMEG : C770_GR4Chr1483 C770_GR4pC1394
SMEL : SM2011_a0093 SM2011_c01047
SMER : DU99_07665 DU99_25220
SAME : SAMCFNEI73_Ch1592(dat)
EAK : EKH55_1239 EKH55_1842
EMX : FKV68_07870 FKV68_24890
SKM : PZL22_001624 PZL22_005685
EAD : OV14_2537 OV14_a0195
EAH : FA04_06125 FA04_21190
ECAA : J3R84_06065 J3R84_26870
ENP : JVX98_02945 JVX98_14470
ATU : Atu2511(dat) Atu5473(dat)
AVQ : HRR99_15910 HRR99_21355
ALEG : CFBP4996_01275 CFBP4996_10925
AGRC : GSF67_12140 GSF67_24655
RLS : HB780_10615 HB780_15465
RSUL : N2599_08970 N2599_32380
ARA : Arad_2269(dat) Arad_7091(dat)
NGG : RG540_CH16170 RG540_CH19590
NEO : CYG48_07995 CYG48_16980
NEN : NCHU2750_19800(dat) NCHU2750_49020(dat)
SOJ : K6301_07235 K6301_23355
SSUM : Q9314_00620 Q9314_24095
OIN : IAR37_12275 IAR37_18430
OAN : Oant_1427 Oant_3562 Oant_4609
BPSN : NIK97_05975 NIK97_12800
BRUC : LJ361_06385 LJ361_19130
OCR : HGK82_08900 HGK82_20290 HGK82_24590
OCL : GTN27_09210 GTN27_17575
OCC : KMS41_08195 KMS41_21750
OCH : CES85_1883 CES85_2756
MAQU : Maq22A_1p30695(ilvE)
MLD : U0023_03305 U0023_33825
MTUN : MTUNDRAET4_1124(dat)
TSV : DSM104635_01987(dat)
PPIC : PhaeoP14_02817(dat)
YAN : AYJ57_18180 AYJ57_23475
SINL : DSM14862_03053(dat)
SPSE : SULPSESMR1_03499(dat)
RMM : ROSMUCSMR3_02152(dat)
AHT : ANTHELSMS3_00277(dat)
LALG : LentiSH36_02530(dat)
RSH : Rsph17029_0414 Rsph17029_3100
RSK : RSKD131_0068 RSKD131_3621
PAMI : JCM7686_1436 JCM7686_3170 JCM7686_pAMI5p211
PYE : A6J80_07840 A6J80_19725
PSEW : JHW44_10235 JHW44_19210
PSAP : JHX88_00590 JHX88_01980
LVS : LOKVESSMR4R_02923(dat)
PPRU : FDP22_04800 FDP22_15270
SFLA : SPHFLASMR4Y_01364(dat)
SMUC : JL100_012195 JL100_030155
SROE : JL101_012380 JL101_021190
THAC : CSC3H3_04905 CSC3H3_09615
BAN : BA_2256(dat1) BA_5472(dat2)
BAR : GBAA_2256(dat1) GBAA_5472(dat2)
BAH : BAMEG_2340(dat1) BAMEG_5518(dat2)
BAI : BAA_2318(dat1) BAA_5498(dat2)
BAX : H9401_2141 H9401_5215
BANT : A16_22910 A16_54820
BANR : A16R_23180 A16R_55450
BANS : BAPAT_2155 BAPAT_5244
BANH : HYU01_11245 HYU01_26715
BANV : DJ46_1052(dat) DJ46_4127(dat)
BCA : BCE_2285(dat) BCE_5351(dat)
BCZ : BCE33L2037(daaA) BCE33L4929(dat)
BCR : BCAH187_A2366(dat1) BCAH187_A5405(dat2)
BCB : BCB4264_A2238(dat1) BCB4264_A5356(dat2)
BCU : BCAH820_2281(dat1) BCAH820_5323(dat2)
BCG : BCG9842_B3086(dat1) BCG9842_B5604(dat2)
BCQ : BCQ_2192(dat) BCQ_5062(dat)
BCX : BCA_2339(dat1) BCA_5371(dat2)
BAL : BACI_c22110(daaA) BACI_c52240(dat)
BCER : BCK_09220 BCK_23555
BCEF : BcrFT9_01797 BcrFT9_04187
BCY : Bcer98_1655 Bcer98_3767
BTK : BT9727_2039(daaA) BT9727_4914(dat)
BTL : BALH_2017(daaA) BALH_4731(dat)
BTB : BMB171_C1987(dat1) BMB171_C4828(dat2)
BTHR : YBT1520_12210 YBT1520_27890
BTHI : BTK_12380 BTK_27700
BTC : CT43_CH2174(dat1) CT43_CH5262(dat2)
BTF : YBT020_11560 YBT020_26080
BTG : BTB_c22860(dat1) BTB_c54260(dat2)
BTN : BTF1_08555 BTF1_24540
BTHT : H175_ch2207 H175_ch5351
BTHU : YBT1518_12280 YBT1518_29455
BTW : BF38_3458(dat) BF38_959(dat)
BTHY : AQ980_03160 AQ980_18575
BWE : BcerKBAB4_2079 BcerKBAB4_5026
BWW : bwei_2785(dat1) bwei_4555(dat2)
BMYO : BG05_3779(dat) BG05_816(dat)
BTY : Btoyo_2437 Btoyo_4839
BMYC : DJ92_2284(dat) DJ92_4815(dat)
BBY : CY96_10175 CY96_25210
BWD : CT694_11980(dat) CT694_28150
BTRO : FJR70_08350(dat) FJR70_20295
BMOB : MLA2C4_11285(dat) MLA2C4_27065
BNT : GSN03_10835(dat) GSN03_24870
BPAC : LMD38_00715 LMD38_15480(dat)
BPAN : NLJ82_10895(dat) NLJ82_25765
BALU : QRY64_13570(dat) QRY64_28885
BFD : NCTC4823_01255(dat_1)
BACA : FAY30_22530(dat) FAY30_22615(dat)
BARD : QRY57_12595(dat) QRY57_25550
BKW : BkAM31D_17000(dat_2)
PTL : AOT13_06850 AOT13_14350
LYC : FH508_0001285(dat) FH508_0018310(dat)
VHL : BME96_03985 BME96_14090
VIL : CFK37_01280(dat) CFK37_09280(dat) CFK37_11375(dat)
VNT : OLD84_06575(dat) OLD84_06745(dat)
VIK : KFZ58_06255(dat) KFZ58_06485(dat)
LAO : AOX59_09080 AOX59_17810
BLEN : NCTC4824_03144(dat)
STEA : C0679_04155(dat) C0679_07460(dat)
SAX : USA300HOU_1741(daaA)
SUQ : HMPREF0772_11402(dat)
SUX : SAEMRSA15_16580(dat)
SUF : SARLGA251_16400(dat)
SAUV : SAI7S6_1012870(dat)
SAUW : SAI5S5_1012820(dat)
SAUX : SAI6T6_1012830(dat)
SAUY : SAI8T7_1012860(dat)
SDP : NCTC12225_01249(dat)
SPIC : SAMEA4384060_1209(dat)
SSH : NCTC13712_01742(dat)
SSIM : SAMEA4384339_1633(dat)
SSTE : SAMEA4384403_0987(dat)
LMOC : LMOSLCC5850_1684(dat)
LMW : LMOSLCC2755_1629(dat)
LMX : LMOSLCC2372_1684(dat)
LMZ : LMOSLCC2482_1680(dat)
LMON : LMOSLCC2376_1577(dat)
LMOS : LMOSLCC7179_1593(dat)
LMOO : LMOSLCC2378_1637(dat)
LMOY : LMOSLCC2479_1682(dat)
LMOT : LMOSLCC2540_1699(dat)
LMOA : LMOATCC19117_1631(dat)
LGZ : NCTC10812_01623(dat)
BCOP : JD108_05765(dat) JD108_13330(dat)
BAYD : BSPP4475_11840(dat)
BBOR : RFB14_06190(dat) RFB14_15515(dat)
BRUM : NDK47_06860(dat) NDK47_15965(dat)
PBJ : VN24_06770 VN24_11575 VN24_23485
NTR : B0W44_05555 B0W44_13200
CTYK : CTK_C03180(dat) CTK_C13690
CAUN : CLAUR_015590(dat_2)
TFR : BR63_01410(dat) BR63_09590
NCD : ACONDI_01237(dat_2) ACONDI_01430(dat_3)
VDN : NCTC11831_00408(dat)
PFAC : PFJ30894_01958(dat)
CIU : G4D55_15080(dat) G4D55_17215(dat)
» show all
Taxonomy
Reference
Authors
THORNE CB, GOMEZ CG, HOUSEWRIGHT RD.
Title
Transamination of D-amino acids by Bacillus subtilis.
Journal
Reference
Authors
THORNE CB, MOLNAR DM.
Title
D-Amino acid transamination in bacillus anthracis.
Journal
Reference
Authors
Martinez-Carrion M, Jenkins WT.
Title
D-Alanine-D-glutamate transaminase. I. Purification and characterization.
Journal
J Biol Chem 240:3538-46 (1965)
Reference
Authors
Ogawa T, Fukuda M, Sasaoka K.
Title
Occurrence of D-amino acid aminotransferase in pea seedlings.
Journal
Reference
Authors
Yonaha K, Misono H, Yamamoto T, Soda K.
Title
D-amino acid aminotransferase of Bacillus sphaericus. Enzymologic and spectrometric properties.
Journal
J Biol Chem 250:6983-9 (1975)
Reference
Authors
Tanizawa K, Masu Y, Asano S, Tanaka H, Soda K
Title
Thermostable D-amino acid aminotransferase from a thermophilic Bacillus species. Purification, characterization, and active site sequence determination.
Journal
J Biol Chem 264:2445-9 (1989)
Sequence
Reference
Authors
Fotheringham IG, Bledig SA, Taylor PP
Title
Characterization of the genes encoding D-amino acid transaminase and glutamate racemase, two D-glutamate biosynthetic enzymes of Bacillus sphaericus ATCC 10208.
Journal
Sequence
Reference
Authors
van Ophem PW, Erickson SD, Martinez del Pozo A, Haller I, Chait BT, Yoshimura T, Soda K, Ringe D, Petsko G, Manning JM.
Title
Substrate inhibition of D-amino acid transaminase and protection by salts and by reduced nicotinamide adenine dinucleotide: isolation and initial characterization of a pyridoxo intermediate related to inactivation.
Journal
Reference
Authors
Sugio S, Petsko GA, Manning JM, Soda K, Ringe D
Title
Crystal structure of a D-amino acid aminotransferase: how the protein controls stereoselectivity.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.21
ExPASy - ENZYME nomenclature database: 2.6.1.21
LinkDB
All DBs