KEGG   ORTHOLOGY: K01742
Entry
K01742            Tight     KO                                     
Symbol
THS
Name
thebaine synthase [EC:4.2.99.24]
Pathway
map00950  Isoquinoline alkaloid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00944  Morphine biosynthesis, (S)-reticuline => morphine
Reaction
R04769  salutaridinol 7-O-acetate acetate-lyase (thebaine forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00950 Isoquinoline alkaloid biosynthesis
    K01742  THS; thebaine synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.99  Other carbon-oxygen lyases
    4.2.99.24  thebaine synthase
     K01742  THS; thebaine synthase
Genes
PSOM: 113322169 113340172
Reference
PMID:29807982
  Authors
Chen X, Hagel JM, Chang L, Tucker JE, Shiigi SA, Yelpaala Y, Chen HY, Estrada R, Colbeck J, Enquist-Newman M, Ibanez AB, Cottarel G, Vidanes GM, Facchini PJ
  Title
A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis.
  Journal
Nat Chem Biol 14:738-743 (2018)
DOI:10.1038/s41589-018-0059-7
  Sequence
[psom:113322169]
LinkDB

KEGG   ENZYME: 4.2.99.24
Entry
EC 4.2.99.24                Enzyme                                 
Name
thebaine synthase;
THS
Class
Lyases;
Carbon-oxygen lyases;
Other carbon-oxygen lyases
Sysname
salutaridinol 7-O-acetate acetate-lyase (thebaine-forming)
Reaction(IUBMB)
salutaridinol 7-O-acetate = thebaine + acetate [RN:R04769]
Reaction(KEGG)
R04769
Substrate
salutaridinol 7-O-acetate [CPD:C05322]
Product
thebaine [CPD:C06173];
acetate [CPD:C00033]
Comment
Isolated from the plant Papaver somniferum (opium poppy). The reaction occurs spontaneously when the pH is between 8-9, but the enzyme is required at the physiological pH, which is close to 7.
History
EC 4.2.99.24 created 2019
Pathway
ec00950  Isoquinoline alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K01742  thebaine synthase
Genes
PSOM: 113322169 113340172
Reference
1  [PMID:29807982]
  Authors
Chen X, Hagel JM, Chang L, Tucker JE, Shiigi SA, Yelpaala Y, Chen HY, Estrada R, Colbeck J, Enquist-Newman M, Ibanez AB, Cottarel G, Vidanes GM, Facchini PJ
  Title
A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis.
  Journal
Nat Chem Biol 14:738-743 (2018)
DOI:10.1038/s41589-018-0059-7
  Sequence
[psom:113322169]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.99.24
IUBMB Enzyme Nomenclature: 4.2.99.24
ExPASy - ENZYME nomenclature database: 4.2.99.24
BRENDA, the Enzyme Database: 4.2.99.24
LinkDB

KEGG   REACTION: R04769
Entry
R04769                      Reaction                               
Name
salutaridinol 7-O-acetate acetate-lyase (thebaine forming)
Definition
7-O-Acetylsalutaridinol <=> Thebaine + Acetate
Equation
C05322 <=> C06173 + C00033
Comment
The reaction occurs spontaneously when the pH is between 8-9.
Reaction class
RC00523  C00033_C05322
RC01956  C05322_C06173
Enzyme
4.2.99.24
Pathway
rn00950  Isoquinoline alkaloid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00944  Morphine biosynthesis, (S)-reticuline => morphine
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.2  Carbon-oxygen lyases
   4.2.99  Other carbon-oxygen lyases
    4.2.99.24
     R04769  7-O-Acetylsalutaridinol <=> Thebaine + Acetate
Orthology
K01742  thebaine synthase [EC:4.2.99.24]
Other DBs
RHEA: 56911
LinkDB

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