Entry
Name
cobyrinate a,c-diamide synthase;
cobyrinic acid a,c-diamide synthetase;
CbiA
Class
Ligases;
Forming carbon-nitrogen bonds;
Carbon-nitrogen ligases with glutamine as amido-N-donor
BRITE hierarchy
Sysname
cobyrinate:L-glutamine amido-ligase (ADP-forming)
Reaction(IUBMB)
2 ATP + cobyrinate + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + cobyrinate a,c-diamide + 2 L-glutamate (overall reaction) [RN:
R05815 ];
(1a) ATP + cobyrinate + L-glutamine + H2O = ADP + phosphate + cobyrinate c-monamide + L-glutamate [RN:
R09598 ];
(1b) ATP + cobyrinate c-monamide + L-glutamine + H2O = ADP + phosphate + cobyrinate a,c-diamide + L-glutamate [RN:
R09599 ]
Reaction(KEGG)
Substrate
Product
Comment
This enzyme is the first glutamine amidotransferase that participates in the anaerobic (early cobalt insertion) biosynthetic pathway of adenosylcobalamin, and catalyses the ATP-dependent synthesis of cobyrinate a,c-diamide from cobyrinate using either L-glutamine or ammonia as the nitrogen source. It is proposed that the enzyme first catalyses the amidation of the c-carboxylate, and then the intermediate is released into solution and binds to the same catalytic site for the amidation of the a-carboxylate. The Km for ammonia is substantially higher than that for L-glutamine. The equivalent reaction in the aerobic cobalamin biosynthesis pathway is catalysed by EC
6.3.5.9 , hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing).
History
EC 6.3.5.11 created 2010
Pathway
Orthology
K02224 cobyrinic acid a,c-diamide synthase
Genes
BUF : D8682_20825 D8682_24200
METY : MRY16398_18630(cbiA)
VSR : Vspart_03864(cobB_2)
PAEB : NCGM1900_5301(cobB)
PCQ : PcP3B5_15450(cobB_3)
PPUD : DW66_1246 DW66_1608
PVD : CFBP1590__1584(cobB)
PPRC : PFLCHA0_c45030(cobB3)
PFW : PF1751_v1c39840(cobB)
PPUU : PputUW4_03774(cobB)
PTW : TUM18999_41250(cobB)
PTAE : NCTC10697_01152(cobB3)
PSPO : PSPO_b0394(cobB-cbiA)
METL : U737_07575 U737_13385
TSB : HMY34_13800 HMY34_19350
ALV : Alvin_1263(dsrN) Alvin_1817
ATEP : Atep_14820(cbiA) Atep_20810(cobB)
TVI : Thivi_0556 Thivi_1697
TMB : Thimo_0155 Thimo_0500
MPUR : MARPU_06125 MARPU_10855
TSY : THSYN_22485 THSYN_32050
TTP : E6P07_05610 E6P07_09330(cobB)
THIP : N838_20065 N838_26980
CJAP : GWK36_02350 GWK36_05890
THIM : KFB96_00950 KFB96_10620
HHK : HH1059_02860(cobB_1) HH1059_16660(cobB_3)
TNI : TVNIR_0848(cobB_[H])
GAI : IMCC3135_08970(cobB_2)
HAXI : HAALTHF_42370n(cobB)
NJP : NEJAP_1825(cobB-cbiA)
OME : OLMES_2750 OLMES_2751
BMV : BMASAVP1_A1620(cobB)
BML : BMA10229_A0282(cobB)
BPM : BURPS1710b_2093(cobB)
BPL : BURPS1106A_1948(cobB)
BYI : BYI23_B004030(cobB_cobQ)
BUE : BRPE67_BCDS06630(cobB_cobQ)
PLG : NCTC10937_04027(cobB_2)
LMIR : NCTC12852_01755(cobB_2)
RHF : EUB48_03105 EUB48_08700
HTN : KI616_02575 KI616_14480
PBH : AAW51_2411(cobBcbiA)
MPT : Mpe_B0437(cobB) Mpe_B0472(cobB) Mpe_B0510(cobB)
RBN : RBXJA2T_02512 RBXJA2T_19526
SHD : SUTH_01432(dsrN) SUTH_02591(cobB)
DOE : DENOEST_1191(cobB) DENOEST_2512(cobB)
FAM : OYT1_ch1293 OYT1_ch1904
AZA : AZKH_0617(cbiA_cobB)
FPHO : SHINM1_019370(cobB)
GBN : GEOBRER4_37010(cbiA)
DVU : DVU_0405(cobB-1) DVU_3086(cobB-2)
DVG : Deval_0367 Deval_2850
DFI : AXF13_13790 AXF13_14815
DPG : DESPIGER_0952 DESPIGER_2041
DTR : RSDT_0011(cbiA2) RSDT_0433(cbiA1)
DFL : DFE_2977(cobB) DFE_3073
DSD : GD606_09320 GD606_14675
DMA : DMR_03630 DMR_11850(cbiA)
DCB : C3Y92_15125 C3Y92_18580
DMS : E8L03_10360 E8L03_15030
DSA : Desal_0269 Desal_0790
DHY : DESAM_20114 DESAM_20464(cobB)
DAF : Desaf_1373 Desaf_1831
DPI : BN4_11946(cobB) BN4_12631(cobB)
DEJ : AWY79_04755 AWY79_17885
PPRF : DPRO_1443(cbiA) DPRO_2481(cbiA)
PSEL : GM415_02155 GM415_07950
DDN : DND132_2058 DND132_2812
DSX : GD604_01390 GD604_02685
PBIZ : LWC08_10480 LWC08_12045
DOA : AXF15_07395 AXF15_12570
DAK : DaAHT2_1581 DaAHT2_1671
DPR : Despr_2263 Despr_3025
DEO : CAY53_05230 CAY53_09570
DOG : HP555_01255 HP555_11430
DBK : DGMP_08460(cobB) DGMP_11880(cbiA)
DML : Dmul_28150(cobB) Dmul_30230(cbiB)
DAT : HRM2_00190(cbiA1) HRM2_42370(cbiA2)
DTO : TOL2_C00080(cbiA) TOL2_C05600(cobB)
DOV : DSCO28_30720(cbiA) DSCO28_40970(cobB)
DWD : DSCW_33980(cbiA) DSCW_41430(cobB_2)
DALK : DSCA_41730(cobB_2) DSCA_61570(cbiA)
DLI : dnl_18190(cobB2) dnl_18200(cobB3) dnl_54500(cbiA)
DMM : dnm_050820(cbiA) dnm_070950(cobB2)
DEK : DSLASN_01960(cbiA) DSLASN_38000(cobB)
DAO : Desac_1620 Desac_2242
DTI : Desti_1558 Desti_1640 Desti_5593
DAX : FDQ92_05045 FDQ92_06550
DHR : LGS26_01320 LGS26_06085
SMEL : SM2011_c04282(cobB)
SFD : USDA257_c42250(cobB)
SAME : SAMCFNEI73_Ch2188(cobB)
AGR : AGROH133_09245(cobB)
REC : RHECIAT_CH0002586(cobB)
REL : REMIM1_CH02506(cobB)
REP : IE4803_CH02533(cobB)
REI : IE4771_CH02620(cobB)
RTR : RTCIAT899_CH10565(cobB)
RGA : RGR602_CH02271(cobB)
RPHA : AMC79_CH02613(cobB)
NEN : NCHU2750_36550(cobB)
BIO : BR141012304_11401(cobB)
MAQU : Maq22A_c09485(cobB)
BBAR : RHAL1_00073(cobB_1)
FIL : BN1229_v1_3167(cobB)
FIY : BN1229_v1_2755(cobB)
BLAG : BLTE_14780(cobB_1) BLTE_30720(cbiA)
RLI : RLO149_c006260(cobB)
RED : roselon_02140 roselon_02167
SINL : DSM14862_03164(cobB_2)
SPSE : SULPSESMR1_02797(cobB)
RMM : ROSMUCSMR3_00673(cobB)
RSK : RSKD131_3359 RSKD131_4283
LVS : LOKVESSMR4R_01276(cobB)
GEH : HYN69_05190 HYN69_20040
PAMO : BAR1_04815 BAR1_07330
PALW : PSAL_011270(cobB_1)
NOR : FA702_01445 FA702_20520
EBLA : JGUZn3_06360(cobB_2)
MGY : MGMSRv2__3223(cobB) MGMSRv2__4059(cobB)
MGRY : MSR1_23410(cobB_2) MSR1_29510(cobB_3)
MAGX : XM1_0159(cobB) XM1_1840(cobB)
MAGN : WV31_03225 WV31_16970
MAGQ : MGMAQ_2122(cobB) MGMAQ_3764(cobB)
BKW : BkAM31D_09085(cobB_2)
PCAL : BV455_03716(cobB_3)
LMOC : LMOSLCC5850_1181(cobB)
LMW : LMOSLCC2755_1184(cobB)
LMX : LMOSLCC2372_1188(cobB)
LMZ : LMOSLCC2482_1232(cobB)
LMON : LMOSLCC2376_1142(cobB)
LMOS : LMOSLCC7179_1159(cobB)
LMOO : LMOSLCC2378_1197(cobB)
LMOY : LMOSLCC2479_1189(cobB)
LMOT : LMOSLCC2540_1170(cobB)
LMOA : LMOATCC19117_1191(cobB)
PPQ : PPSQR21_047870(cobB)
AFX : JZ786_00005 JZ786_06520 JZ786_07560 JZ786_18890
SMEN : SAMEA4412692_0352(cobB)
LRU : HMPREF0538_20909(cobB)
AUR : HMPREF9243_1742(cobB)
CPAE : CPAST_c12580(cobB2)
CCOH : SAMEA4530647_0615(cobB_2)
CCL : Clocl_3583 Clocl_3810
BPRO : PMF13cell1_04262(cbiA)
CSCI : HDCHBGLK_00118(cbiA)
ACEL : acsn021_39640(cobB)
ACHT : bsdcttw_22340(cbiA)
PDF : CD630DERM_34340(cobB)
EAC : EAL2_808p00970(cobB)
PHX : KGNDJEFE_00476(cbiA)
DSY : DSY0318(cobB) DSY4060(cobB)
DDH : Desde_0276 Desde_1238
DDL : Desdi_0252 Desdi_1029
DOR : Desor_2795 Desor_3300
DAI : Desaci_2444 Desaci_3002
DMI : Desmer_2198 Desmer_2564
DEC : DCF50_p63 DCF50_p808(cobB)
DCA : Desca_0578 Desca_2676
DRU : Desru_0898 Desru_3733
DFG : B0537_13960 B0537_15890
DKU : Desku_2368 Desku_3484
TFR : BR63_04695 BR63_06715(cobB)
FWA : DCMF_23770 DCMF_28240
AACX : DEACI_0678 DEACI_2582
DGI : Desgi_1894 Desgi_4647
CBAR : PATL70BA_0217(cbiA)
ETM : CE91St48_21020(cobB_2)
CHY : CHY_0771(cobB1) CHY_2399(cobB2)
MTHO : MOTHE_c10600(cobB1) MOTHE_c16340(cobB2)
MTHZ : MOTHA_c11490(cobB2) MOTHA_c17170(cobB3)
PIV : NCTC13079_00950(cobB_1)
VRM : 44547418_01198(cobB)
VDN : NCTC11831_00762(cobB)
DHO : Dia5BBH33_09950(cbiA)
MANA : MAMMFC1_03999(cobB)
PFAC : PFJ30894_01754(cobB)
MBO : BQ2027_MB2873C(cobB)
MMAE : MMARE11_18130(cobB)
MBAI : MB901379_01708(cobB_1)
MHEK : JMUB5695_03238(cobB_3)
MGAU : MGALJ_07890(cobB_1)
MPHL : MPHLCCUG_03209(cobB_1)
MTHN : 4412656_01686(cobB_1)
MCHT : MCHIJ_44830(cobB_3)
MAUU : NCTC10437_02166(cobB_1)
MPSC : MPSYJ_43710(cobB_2)
MMAT : MMAGJ_56050(cobB_1)
MSEI : MSEDJ_24730(cobB_1)
MFLV : NCTC10271_03217(cobB_3)
MSAL : DSM43276_02947(cobB_2)
MTER : 4434518_03156(cobB_3)
CDIP : ERS451417_01513(cobB)
CUN : Cul210932_1468(cobB)
CUQ : Cul210931_1356(cobB)
CUJ : CUL131002_1389c(cobB)
NFR : ERS450000_05833(cobB_3)
NAD : NCTC11293_05280(cobB_3)
NWL : NWFMUON74_22390(cobB_2)