KEGG ORTHOLOGY: K04121

ORTHOLOGY: K04121

Entry

K04121                      KO

Symbol

E4.2.3.19

Name

ent-kaurene synthase [EC:4.2.3.19]

Pathway

map00904

Diterpenoid biosynthesis

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Module

M00927

Gibberellin A12 biosynthesis, GAPP => GA12

Reaction

R05092

ent-copalyl-diphosphate diphosphate-lyase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K04121  E4.2.3.19; ent-kaurene synthase
Enzymes [BR:ko01000]
4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.19  ent-kaurene synthase
     K04121  E4.2.3.19; ent-kaurene synthase

Other DBs

GO:

0009899

Genes

ATH:	AT1G79460(GA2)

ALY:

9323851

CRB:

17894494

CSAT:

104703299 104714240 104752382

EUS:	EUTSA_v10018144mg

BRP:

103832422

BNA:	106354788 106430723

BOE:

RSZ:

THJ:

CPAP:

CIT:

CIC:	CICLE_v10006575mg

PVY:	116142016 116142017 116142045 116142046

MINC:

123197853

TCC:

18594964

GRA:

105782971

GHI:	107916812 107921168

GAB:

108480537

DZI:

111287930

EGR:

104450546

GMX:	100793849(DW1)

GSJ:

114422065

PVU:	PHAVU_005G048500g

VRA:

VAR:

VUN:

VUM:

CCAJ:

APRC:

MTR:	11446526 25486907

TPRA:

123893887 123893891

CAM:

101506236

PSAT:

127134672

VVO:	131594659 131624658

LJA:	130749014 130749015

ADU:

107458750

AIP:

107613546

AHF:	112702964 112771489

LANG:

109356576

PCIN:

129297678 129299288 129314362 129314986 129315049 129315377 129316106 129316172 129316209 129316245 129316719 129316883

QSA:	O6P43_001250 O6P43_001251 O6P43_001255 O6P43_001257 O6P43_001259 O6P43_012828 O6P43_014414 O6P43_029401

FVE:	101301279 101301569

RCN:

112164573

PPER:

18778990

PMUM:

103325592 103325640 103343621

PAVI:

110745425 110767389 110770543 110770546

PDUL:

117625499 117626735

MDM:	103400427 103407925 103415767 103445924(KS)

MSYL:

126586842 126617088

PXB:	103932282 103932630 103937596

ZJU:

MNT:

CSAV:

CSV:

CMO:

BHJ:

MCHA:

CMAX:

CMOS:

CPEP:

RCU:	8259343 8259388 8277650 8282984 8282989 8282990

MEAN:

126662243 126665727 126681455 126681975 126682807 126682901 126683157 130014496

JCU:	105631138 105649262

HBR:	110633380 110633381 110646746 110660499 110660517 110662210 110667076 110671325 110672862

MESC:

110602995 110603473 110603522 110604205 110618412 110619742

POP:

18101433

PEU:

105118102

PALZ:

118055753

JRE:	108998981 108998982

CILL:

122280447

CAVE:

132176499

QLO:

115983925

TWL:	119988173 119988174 119988175

VVI:

100267397

VRI:

117909326

SLY:	101252605(TPS24)

SPEN:

SOT:

SSTN:

SDUL:

CANN:

LBB:

NTA:	107795749(NtKS) 107825637

NSY:

NTO:

NAU:

INI:

ITR:

SIND:

EGT:	105968700 105968701 105971074

APAN:

127247477

HAN:	110917813 110918013 110918028 110918153 110928588

ECAD:

122595277 122595279 122597925 122609084

LSV:	111876762 111876799

CCAV:

112522554 112522847

DCR:	108219753 108222343

CSIN:

RVL:

AEW:

BVG:

SOE:

CQI:	110682252 110718729

ATRI:

MOF:

NNU:

MING:

TSS:

PSOM:

OSA:	4336960(ks1)

OBR:

102721543

OGL:

127770560

BDI:	100827748 100828967 100829880

ATS:	109754648 109764882 109780574 109780577 109780593

TDC:	119323942 119356230 119358107 119358108 119358112 119358255 119358647 119361095 119361107 119361808 119365394 119365395 119365400

TAES:

100873131 100873134 100873147 123039360 123039367 123039521 123046477 123050317 123055900 123055901 123055905 123133218 123190211 123191848 123191853 123191952

TUA:	125534266 125537707 125537728 125539505 125539514

LPER:

127296660 127330960 127332585 127336225 127336228 127336230 127341094

LRD:	124671374 124672807 124674790 124675518 124677323 124679045 124694643 124703190 124704756

SBI:

8063956

ZMA:	103645955 109943181

MFLO:

136493648 136493708 136497414

SITA:

101753442 101772220

SVS:	117844058 117864176

PVIR:

120641744 120658831 120666048 120666049 120666116 120666349 120666350 120667842 120682376 120699635 120701539

PHAI:

112886849 112887158 112899736

PDA:	103698812 103720937 103722282 120106536 120106542 120108804

EGU:	105059898 105059899 105059986

MUS:

103969833

ZOF:	121970368 121970373 121970374 121972559 121975450 121975451 121977709 121977710 121977711

DCT:

110092854

PEQ:	110024106 110030130 110039138

AOF:

109851541

MSIN:

131221117

NCOL:

116254598

ATR:	105421340 18443240

» show all

Reference

PMID:17456599

Authors

Xu M, Wilderman PR, Peters RJ

Title

Following evolution's lead to a single residue switch for diterpene synthase product outcome.

Journal

Proc Natl Acad Sci U S A 104:7397-401 (2007)
DOI:10.1073/pnas.0611454104

Reference

PMID:9536043

Authors

Yamaguchi S, Sun Tp, Kawaide H, Kamiya Y

Title

The GA2 locus of Arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis.

Journal

Plant Physiol 116:1271-8 (1998)
DOI:10.1104/pp.116.4.1271

Sequence

[ath:AT1G79460]

LinkDB

ORTHOLOGY: K20657

Entry

K20657                      KO

Symbol

CPS-KS

Name

ent-copalyl diphosphate/ent-kaurene synthase [EC:5.5.1.13 4.2.3.19]

Pathway

map00904

Diterpenoid biosynthesis

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Module

M00929

Gibberellin A1 biosynthesis, GGPP => GA1

Reaction

R02068

ent-copalyl-diphosphate lyase

R05092

ent-copalyl-diphosphate diphosphate-lyase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K20657  CPS-KS; ent-copalyl diphosphate/ent-kaurene synthase
Enzymes [BR:ko01000]
4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.19  ent-kaurene synthase
     K20657  CPS-KS; ent-copalyl diphosphate/ent-kaurene synthase
5. Isomerases
  5.5  Intramolecular lyases
   5.5.1  Intramolecular lyases (only sub-subclass identified to date)
    5.5.1.13  ent-copalyl diphosphate synthase
     K20657  CPS-KS; ent-copalyl diphosphate/ent-kaurene synthase

Other DBs

GO:	0009905 0009899

Genes

MGR:

MGG_01949

PPEI:

PpBr36_10643

PGRI:

PgNI_12055 PgNI_12444

FMU:	J7337_005030

TASP:

36612063(TrAFT101_010790)

PCHM:

VFPPC_09474

BZE:	COCCADRAFT_113213

BSC:	COCSADRAFT_104215

BOR:	COCMIDRAFT_30863

AG:	BAA22426 BAA84917(CPS)

Reference

PMID:9268298

Authors

Kawaide H, Imai R, Sassa T, Kamiya Y

Title

Ent-kaurene synthase from the fungus Phaeosphaeria sp. L487. cDNA isolation, characterization, and bacterial expression of a bifunctional diterpene cyclase in fungal gibberellin biosynthesis.

Journal

J Biol Chem 272:21706-12 (1997)
DOI:10.1074/jbc.272.35.21706

Sequence

[ag:BAA22426]

LinkDB

ORTHOLOGY: K27636

Entry

K27636            Tight     KO

Symbol

CPS-KS

Name

ent-copalyl diphosphate/ent-kaurene/ent-beyerene synthase [EC:5.5.1.13 4.2.3.19 4.2.3.229]

Pathway

map00904

Diterpenoid biosynthesis

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Reaction

R02068

ent-copalyl-diphosphate lyase

R05092

ent-copalyl-diphosphate diphosphate-lyase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K27636  CPS-KS; ent-copalyl diphosphate/ent-kaurene/ent-beyerene synthase
Enzymes [BR:ko01000]
4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.19  ent-kaurene synthase
     K27636  CPS-KS; ent-copalyl diphosphate/ent-kaurene/ent-beyerene synthase
    4.2.3.229  ent-beyerene synthase
     K27636  CPS-KS; ent-copalyl diphosphate/ent-kaurene/ent-beyerene synthase
5. Isomerases
  5.5  Intramolecular lyases
   5.5.1  Intramolecular lyases (only sub-subclass identified to date)
    5.5.1.13  ent-copalyl diphosphate synthase
     K27636  CPS-KS; ent-copalyl diphosphate/ent-kaurene/ent-beyerene synthase

Other DBs

GO:	0009905 0009899

Genes

PPP:

112284636

Reference

PMID:26248039

Authors

Zhan X, Bach SS, Hansen NL, Lunde C, Simonsen HT.

Title

Additional diterpenes from Physcomitrella patens synthesized by copalyl diphosphate/kaurene synthase (PpCPS/KS).

Journal

Plant Physiol Biochem 96:110-4 (2015)
DOI:10.1016/j.plaphy.2015.07.011

Sequence

[ppp:112284636]

LinkDB

ENZYME: 4.2.3.19

Entry

EC 4.2.3.19                 Enzyme

Name

ent-kaurene synthase;
ent-kaurene synthase B;
ent-kaurene synthetase B, ent-copalyl-diphosphate diphosphate-lyase (cyclizing)

Class

Lyases;
Carbon-oxygen lyases;
Acting on phosphates

Sysname

ent-copalyl-diphosphate diphosphate-lyase (cyclizing, ent-kaurene-forming)

Reaction(IUBMB)

ent-copalyl diphosphate = ent-kaurene + diphosphate [RN:R05092]

Reaction(KEGG)

R05092

Substrate

ent-copalyl diphosphate [CPD:C06089]

Product

ent-kaurene [CPD:C06090];
diphosphate [CPD:C00013]

Comment

Part of a bifunctional enzyme involved in the biosynthesis of ent-kaurene. See also EC 5.5.1.13 (ent-copalyl diphosphate synthase)

History

EC 4.2.3.19 created 2002

Pathway

ec00904

Diterpenoid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K04121

ent-kaurene synthase

K20657

ent-copalyl diphosphate/ent-kaurene synthase

K27636

ent-copalyl diphosphate/ent-kaurene/ent-beyerene synthase

Genes

ATH:	AT1G79460(GA2)

ALY:

9323851

CRB:

17894494

CSAT:

104703299 104714240 104752382

EUS:	EUTSA_v10018144mg

BRP:

103832422

BNA:	106354788 106430723

BOE:

106297063

RSZ:

108826853

THJ:

104812382

CPAP:

110817263

CIT:

102609462

CIC:	CICLE_v10006575mg

PVY:	116142016 116142017 116142045 116142046

MINC:

123197853

TCC:

18594964

GRA:

105782971

GHI:	107916812 107921168

GAB:

108480537

DZI:

111287930

EGR:

104450546

GMX:	100793849(DW1)

GSJ:

114422065

PVU:	PHAVU_005G048500g

VRA:

106760976

VAR:

108332359

VUN:

114176685

VUM:

124838687

CCAJ:

109816872

APRC:

113855184

MTR:	11446526 25486907

TPRA:

123893887 123893891

CAM:

101506236

PSAT:

127134672

VVO:	131594659 131624658

LJA:	130749014 130749015

ADU:

107458750

AIP:

107613546

AHF:	112702964 112771489

LANG:

109356576

PCIN:

129297678 129299288 129314362 129314986 129315049 129315377 129316106 129316172 129316209 129316245 129316719 129316883

QSA:	O6P43_001250 O6P43_001251 O6P43_001255 O6P43_001257 O6P43_001259 O6P43_012828 O6P43_014414 O6P43_029401

FVE:	101301279 101301569

RCN:

112164573

PPER:

18778990

PMUM:

103325592 103325640 103343621

PAVI:

110745425 110767389 110770543 110770546

PDUL:

117625499 117626735

MDM:	103400427 103407925 103415767 103445924(KS)

MSYL:

126586842 126617088

PXB:	103932282 103932630 103937596

ZJU:

107424646

MNT:

21402193

CSAV:

115712980

CSV:

101217426

CMO:

103489622

BHJ:

120077990

MCHA:

111016726

CMAX:

111468079

CMOS:

111455458

CPEP:

111811232

RCU:	8259343 8259388 8277650 8282984 8282989 8282990

MEAN:

126662243 126665727 126681455 126681975 126682807 126682901 126683157 130014496

JCU:	105631138 105649262

HBR:	110633380 110633381 110646746 110660499 110660517 110662210 110667076 110671325 110672862

MESC:

110602995 110603473 110603522 110604205 110618412 110619742

POP:

18101433

PEU:

105118102

PALZ:

118055753

JRE:	108998981 108998982

CILL:

122280447

CAVE:

132176499

QLO:

115983925

TWL:	119988173 119988174 119988175

VVI:

100267397

VRI:

117909326

SLY:	101252605(TPS24)

SPEN:

107026218

SOT:

102602928

SSTN:

125876304

SDUL:

129888827 129891651

CANN:

107878924

LBB:

132617742

NTA:	107795749(NtKS) 107825637

NSY:

104222495

NTO:

104102231

NAU:

109234174

INI:

109156386

ITR:

115998579

SIND:

105175114

EGT:	105968700 105968701 105971074

APAN:

127247477

HAN:	110917813 110918013 110918028 110918153 110928588

ECAD:

122595277 122595279 122597925 122609084

LSV:	111876762 111876799

CCAV:

112522554 112522847

DCR:	108219753 108222343

CSIN:

114262481 114279873

RVL:

131329521

AEW:

130772012

BVG:

104904107

SOE:

110779336

CQI:	110682252 110718729

ATRI:

130825394

MOF:

131166264

NNU:

104599747

MING:

122058922 122060619

TSS:

122651279

PSOM:

113336662

OSA:	4336960(ks1)

OBR:

102721543

OGL:

127770560

BDI:	100827748 100828967 100829880

ATS:	109754648 109764882 109780574 109780577 109780593

TDC:	119323942 119356230 119358107 119358108 119358112 119358255 119358647 119361095 119361107 119361808 119365394 119365395 119365400

TAES:

100873131 100873134 100873147 123039360 123039367 123039521 123046477 123050317 123055900 123055901 123055905 123133218 123190211 123191848 123191853 123191952

TUA:	125534266 125537707 125537728 125539505 125539514

LPER:

127296660 127330960 127332585 127336225 127336228 127336230 127341094

LRD:	124671374 124672807 124674790 124675518 124677323 124679045 124694643 124703190 124704756

SBI:

8063956

ZMA:	103645955 109943181

MFLO:

136493648 136493708 136497414

SITA:

101753442 101772220

SVS:	117844058 117864176

PVIR:

120641744 120658831 120666048 120666049 120666116 120666349 120666350 120667842 120682376 120699635 120701539

PHAI:

112886849 112887158 112899736

PDA:	103698812 103720937 103722282 120106536 120106542 120108804

EGU:	105059898 105059899 105059986

MUS:

103969833

ZOF:	121970368 121970373 121970374 121972559 121975450 121975451 121977709 121977710 121977711

DCT:

110092854

PEQ:	110024106 110030130 110039138

AOF:

109851541

MSIN:

131221117

NCOL:

116254598

ATR:	105421340 18443240

PPP:

112284636

MGR:

MGG_01949

PPEI:

PpBr36_10643

PGRI:

PgNI_12055 PgNI_12444

FMU:	J7337_005030

TASP:

36612063(TrAFT101_010790)

PCHM:

VFPPC_09474

BZE:	COCCADRAFT_113213

BSC:	COCSADRAFT_104215

BOR:	COCMIDRAFT_30863

» show all

Reference

1 [PMID:4331199]

Authors

Fall RR, West CA.

Title

Purification and properties of kaurene synthetase from Fusarium moniliforme.

Journal

J Biol Chem 246:6913-28 (1971)

Reference

2 [PMID:8771778]

Authors

Yamaguchi S, Saito T, Abe H, Yamane H, Murofushi N, Kamiya Y.

Title

Molecular cloning and characterization of a cDNA encoding the gibberellin biosynthetic enzyme ent-kaurene synthase B from pumpkin (Cucurbita maxima L.).

Journal

Plant J 10:203-13 (1996)
DOI:10.1046/j.1365-313X.1996.10020203.x

Reference

3 [PMID:9268298]

Authors

Kawaide H, Imai R, Sassa T, Kamiya Y

Title

Ent-kaurene synthase from the fungus Phaeosphaeria sp. L487. cDNA isolation, characterization, and bacterial expression of a bifunctional diterpene cyclase in fungal gibberellin biosynthesis.

Journal

J Biol Chem 272:21706-12 (1997)
DOI:10.1074/jbc.272.35.21706

Sequence

[ag:BAA22426]

Reference

4 [PMID:10803977]

Authors

Toyomasu T, Kawaide H, Ishizaki A, Shinoda S, Otsuka M, Mitsuhashi W, Sassa T

Title

Cloning of a full-length cDNA encoding ent-kaurene synthase from Gibberella fujikuroi: functional analysis of a bifunctional diterpene cyclase.

Journal

Biosci Biotechnol Biochem 64:660-4 (2000)
DOI:10.1271/bbb.64.660

Sequence

[ag:BAA84917]

Other DBs

ExplorEnz - The Enzyme Database:

4.2.3.19

IUBMB Enzyme Nomenclature:

4.2.3.19

ExPASy - ENZYME nomenclature database:

4.2.3.19

BRENDA, the Enzyme Database:

4.2.3.19

CAS:

9055-64-5

LinkDB

REACTION: R05092

Entry

R05092                      Reaction

Name

ent-copalyl-diphosphate diphosphate-lyase

Definition

ent-Copalyl diphosphate <=> ent-Kaurene + Diphosphate

Equation

C06089 <=> C06090 + C00013

Comment

multi-step reaction
following after R02068

Reaction class

RC01263

C06089_C06090

Enzyme

4.2.3.19

Pathway

rn00904

Diterpenoid biosynthesis

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

Module

M00927

Gibberellin A12 biosynthesis, GAPP => GA12

M00929

Gibberellin A1 biosynthesis, GGPP => GA1

Brite

Enzymatic reactions [BR:br08201]
4. Lyase reactions
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.19
     R05092  ent-Copalyl diphosphate <=> ent-Kaurene + Diphosphate

Orthology

K04121

ent-kaurene synthase [EC:4.2.3.19]

K20657

ent-copalyl diphosphate/ent-kaurene synthase [EC:5.5.1.13 4.2.3.19]

K27636

ent-copalyl diphosphate/ent-kaurene/ent-beyerene synthase [EC:5.5.1.13 4.2.3.19 4.2.3.229]

Other DBs

RHEA:

19528 22223

LinkDB

DBGET integrated database retrieval system