KEGG   ORTHOLOGY: K04122
Entry
K04122                      KO                                     
Symbol
GA3, CYP701
Name
ent-kaurene oxidase [EC:1.14.14.86]
Pathway
map00904  Diterpenoid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00927  Gibberellin A12 biosynthesis, GAPP => GA12
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K04122  GA3, CYP701; ent-kaurene oxidase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K04122  GA3, CYP701; ent-kaurene oxidase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.86  ent-kaurene monooxygenase
     K04122  GA3, CYP701; ent-kaurene oxidase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP701 family
   K04122  GA3, CYP701; ent-kaurene oxidase
Other DBs
RN: R06291 R06292 R06293 R10066
GO: 0052615
Genes
CRG: 105335893 117682816
ATH: AT5G25900(GA3)
ALY: 9308254
CRB: 17884655
CSAT: 104706907 104736615 104771068
EUS: EUTSA_v10004043mg
BRP: 103874435
BNA: 106348557 106348758
BOE: 106301555
RSZ: 108833173 108853944
PVY: 116137812
MINC: 123229342
TCC: 18591283
GRA: 105774074
DZI: 111312649
GMX: 100802970(CYP701A16) 100814223
VRA: 106762922
VAR: 108336168
VUN: 114194654
APRC: 113857239
LJA: Lj0g3v0151459.1(Lj0g3v0151459.1) Lj0g3v0218449.1(Lj0g3v0218449.1) Lj3g3v0002210.2(Lj3g3v0002210.2)
FVE: 101315406
RCN: 112172744
PPER: 18791732
PAVI: 110762553
PDUL: 117616384
ZJU: 107420337
MNT: 21397575
CSV: 101209283
CMO: 103493505
BHJ: 120086045
MCHA: 111010641
RCU: 8260887
JCU: 105633700(EKO)
MESC: 110614910
POP: 18096306
PEU: 105120662
PALZ: 118028112
JRE: 108992364
TWL: 120009866
VVI: 100255742
VRI: 117907815
SLY: 101267112
SPEN: 107016073
NSY: 104230322
NTO: 104089658
NAU: 109224931
INI: 109149104(EKO1)
ITR: 116023321
EGT: 105965462
BVG: 104883634
SOE: 110783160
NNU: 104595848
TSS: 122662774
NCOL: 116255470
DOSA: Os06t0569900-01(Os06g0569900) Os06t0570100-01(Os06g0570100)
OBR: 102719778
SBI: 8065021
MUS: 103986614
AOF: 109822294
 » show all
Reference
PMID:9671797 (CYP701A3)
  Authors
Helliwell CA, Sheldon CC, Olive MR, Walker AR, Zeevaart JA, Peacock WJ, Dennis ES
  Title
Cloning of the Arabidopsis ent-kaurene oxidase gene GA3.
  Journal
Proc Natl Acad Sci U S A 95:9019-24 (1998)
DOI:10.1073/pnas.95.15.9019
  Sequence
[ath:AT5G25900]
LinkDB

KEGG   ORTHOLOGY: K21292
Entry
K21292                      KO                                     
Symbol
CYP503
Name
ent-kaurene oxidase [EC:1.14.14.86]
Pathway
map00904  Diterpenoid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00929  Gibberellin A1 biosynthesis, GGPP => GA1
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K21292  CYP503; ent-kaurene oxidase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K21292  CYP503; ent-kaurene oxidase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.86  ent-kaurene monooxygenase
     K21292  CYP503; ent-kaurene oxidase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, fungi type
  CYP503 family
   K21292  CYP503; ent-kaurene oxidase
Other DBs
RN: R06291 R06292 R06293 R10066
COG: COG2124
GO: 0052615
Genes
FVR: FVEG_16575
TRE: TRIREDRAFT_56687
TRR: M419DRAFT_127652
BFU: BCIN_01g04880
ANI: AN1598.2
NFI: NFIA_009830
ANG: ANI_1_1208164(An18g02700) ANI_1_2452094(An11g09260)
ALUC: AKAW2_11919S AKAW2_21192A
APUU: APUU_11322A APUU_22206A APUU_31775A
TRG: TRUGW13939_10606
ABE: ARB_00548
TVE: TRV_00376
PTE: PTT_08290
ZTR: MYCGRDRAFT_34461(CYP-58)
AG: CAA76703(CYP503A1)
 » show all
Reference
PMID:11472927 (CYP503A1)
  Authors
Tudzynski B, Hedden P, Carrera E, Gaskin P
  Title
The P450-4 gene of Gibberella fujikuroi encodes ent-kaurene oxidase in the gibberellin biosynthesis pathway.
  Journal
Appl Environ Microbiol 67:3514-22 (2001)
DOI:10.1128/AEM.67.8.3514-3522.2001
  Sequence
LinkDB

KEGG   ENZYME: 1.14.14.86
Entry
EC 1.14.14.86               Enzyme                                 
Name
ent-kaurene monooxygenase;
ent-kaurene oxidase (misleading)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
ent-kaur-16-ene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (hydroxylating)
Reaction(IUBMB)
ent-kaur-16-ene + 3 [reduced NADPH---hemoprotein reductase] + 3 O2 = ent-kaur-16-en-19-oate + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O (overall reaction) [RN:R10066];
(1a) ent-kaur-16-ene + [reduced NADPH---hemoprotein reductase] + O2 = ent-kaur-16-en-19-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R06291];
(1b) ent-kaur-16-en-19-ol + [reduced NADPH---hemoprotein reductase] + O2 = ent-kaur-16-en-19-al + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R06292];
(1c) ent-kaur-16-en-19-al + [reduced NADPH---hemoprotein reductase] + O2 = ent-kaur-16-en-19-oate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R06293]
Reaction(KEGG)
Substrate
ent-kaur-16-ene [CPD:C06090];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
ent-kaur-16-en-19-ol [CPD:C11872];
ent-kaur-16-en-19-al [CPD:C11873]
Product
ent-kaur-16-en-19-oate [CPD:C11874];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
ent-kaur-16-en-19-ol [CPD:C11872];
ent-kaur-16-en-19-al [CPD:C11873]
Comment
A cytochrome P-450 (heme thiolate) protein found in plants. Catalyses three successive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid.
History
EC 1.14.14.86 created 2002 as EC 1.14.13.78, transferred 2018 to EC 1.14.14.86
Pathway
ec00904  Diterpenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K04122  ent-kaurene oxidase
K21292  ent-kaurene oxidase
Genes
CRG105335893 117682816
ATHAT5G25900(GA3)
ALY9308254
CRB17884655
CSAT104706907 104736615 104771068
EUSEUTSA_v10004043mg
BRP103874435
BNA106348557 106348758
BOE106301555
RSZ108833173 108853944
THJ104803409 104806957
CPAP110825592 110825600 110825618
CIT102626273 102627235
CICCICLE_v10000884mg CICLE_v10000902mg CICLE_v10001179mg
PVY116137812
MINC123229342
TCC18591283
GRA105774074
GHI107935613 121218163
GAB108471487 108480403
DZI111312649
EGR104449362 104449363
GMX100802970(CYP701A16) 100814223
GSJ114377322 114388284
PVUPHAVU_005G183600g
VRA106762922
VAR108336168
VUN114194654
CCAJ109812642 109817002
APRC113857239
MTRMTR_2g105360
CAM101492348 101492682
LJALj0g3v0151459.1(Lj0g3v0151459.1) Lj0g3v0218449.1(Lj0g3v0218449.1) Lj3g3v0002210.2(Lj3g3v0002210.2)
ADU107459820 107483258
AIP107618674 107638960
AHF112703820 112741508 112771108 112796212
LANG109337304 109337306 109350784
FVE101315406
RCN112172744
PPER18791732
PMUM103320777 103320921
PAVI110762553
PDUL117616384
MDM103441571 103441598
PXB103954018 103955469 108865311
ZJU107420337
MNT21397575
CSV101209283
CMO103493505
BHJ120086045
MCHA111010641
CMAX111476578 111481118
CMOS111442772 111446712
CPEP111788623 111797494
RCU8260887
JCU105633700(EKO)
HBR110636133 110636190
MESC110614910
POP18096306
PEU105120662
PALZ118028112
JRE108992364
QSU112002318 112032780 112032781
QLO115974246 115977868
TWL120009866
VVI100255742
VRI117907815
SLY101267112
SPEN107016073
SOT102588924 102589573
CANN107848806 107867102
NTA107766539 107772573
NSY104230322
NTO104089658
NAU109224931
INI109149104(EKO1)
ITR116023321
SIND105159190 105159193 105166213
OEU111381791 111394477
EGT105965462
SSPL121766036 121802241 121809438
HAN110879488 110879531 110879533 110899627 110899628 118479237
ECAD122579177 122589190
LSV111901864 111912922 111912924
CCAV112503346 112503348 112503349 112514541
DCR108192836 108221827 108222415 108223735 108226232 108226927
CSIN114260575 114314457
BVG104883634
SOE110783160
CQI110692482 110727365
NNU104595848
MING122068825 122076995
TSS122662774
PSOM113286201 113294049 113294437 113330131 113337380 113345845
NCOL116255470
OSA4341347 9269379
DOSAOs06t0569900-01(Os06g0569900) Os06t0570100-01(Os06g0570100)
OBR102719778
BDI100826923 100828145 100834998
ATS109764879 109786379
TDC119331213 119332455 119340853 119351884 119365396 119368209 119368221
TAES123041900 123041901 123046473 123049868 123148035 123152475 123161689 123168202 123185940
SBI8065021
ZMA100274015 103638615
SITA101769883 101770285 101770692
SVS117851887 117853039 117853040
PVIR120670617 120670803
PHAI112889472 112889821
PDA103702042 103709580
EGU105040426 105047970
MUS103986614
DCT110093775 110099182
PEQ110032443 110032601
AOF109822294
ATR18425989 18443400
SMOSELMODRAFT_119177 SELMODRAFT_74427(CYP701C1)
PPP112273131 112273132
FVRFVEG_16575
TRETRIREDRAFT_56687
TRRM419DRAFT_127652
BFUBCIN_01g04880
ANIAN1598.2
NFINFIA_009830
ANGANI_1_1208164(An18g02700) ANI_1_2452094(An11g09260)
ALUCAKAW2_11919S AKAW2_21192A
APUUAPUU_11322A APUU_22206A APUU_31775A
TRGTRUGW13939_10606
CIMCIMG_03089
CPWCPC735_010550
UREUREG_02869
ABEARB_00548
TVETRV_00376
PTEPTT_08290
BZECOCCADRAFT_112173
BSCCOCSADRAFT_185928
BORCOCMIDRAFT_109515 COCMIDRAFT_6219
ZTRMYCGRDRAFT_34461(CYP-58)
NPAUCRNP2_330
 » show all
Reference
1  [PMID:2223832]
  Authors
Ashman PJ, Mackenzie A, Bramley PM.
  Title
Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi.
  Journal
Biochim Biophys Acta 1036:151-7 (1990)
DOI:10.1016/0304-4165(90)90027-T
Reference
2  [PMID:1397591]
  Authors
Archer C, Ashman PJ, Hedden P, Bowyer JR, Bramley PM.
  Title
Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima.
  Journal
Biochem Soc Trans 20:218S (1992)
DOI:10.1042/bst020218s
Reference
3  [PMID:9952446]
  Authors
Helliwell CA, Poole A, Peacock WJ, Dennis ES.
  Title
Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis.
  Journal
Plant Physiol 119:507-10 (1999)
DOI:10.1104/pp.119.2.507
  Sequence
[ath:AT5G25900]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.86
IUBMB Enzyme Nomenclature: 1.14.14.86
ExPASy - ENZYME nomenclature database: 1.14.14.86
BRENDA, the Enzyme Database: 1.14.14.86
CAS: 149565-67-3
LinkDB

KEGG   REACTION: R06292
Entry
R06292                      Reaction                               
Name
ent-Kaur-16-ene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (hydroxylating)
Definition
Kaur-16-en-18-ol + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> Kaur-16-en-18-al + [Oxidized NADPH---hemoprotein reductase] + 2 H2O
Equation
Comment
the second step of three-step reaction (see R10066, R06291+R06292+R06293)
Reaction class
RC00893  C11872_C11873
Enzyme
Pathway
rn00904  Diterpenoid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00927  Gibberellin A12 biosynthesis, GAPP => GA12
M00929  Gibberellin A1 biosynthesis, GGPP => GA1
Orthology
K04122  ent-kaurene oxidase [EC:1.14.14.86]
K21292  ent-kaurene oxidase [EC:1.14.14.86]
Other DBs
RHEA: 21307
LinkDB

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