KEGG ORTHOLOGY: K05551

ORTHOLOGY: K05551

Entry

K05551                      KO

Symbol

actI1, oxyA, tcmK, snoa1, aknB, mtmP

Name

minimal PKS ketosynthase (KS/KS alpha) [EC:2.3.1.- 2.3.1.260 2.3.1.235]

Pathway

map00253

Tetracycline biosynthesis

map01056

Biosynthesis of type II polyketide backbone

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Module

M00778

Type II polyketide backbone biosynthesis, acyl-CoA + malonyl-CoA => polyketide

Reaction

R06635

R06637

R06641

R06643

R06644

R06645

R09258

R09259

R10960

malonyl-CoA:acetate malonyltransferase (tetracenomycin-F2-forming)

R11516

malonyl-CoA:malonamoyl-[acyl-carrier protein] malonyltransferase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01056 Biosynthesis of type II polyketide backbone
    K05551  actI1, oxyA, tcmK, snoa1, aknB, mtmP; minimal PKS ketosynthase (KS/KS alpha)
   00253 Tetracycline biosynthesis
    K05551  actI1, oxyA, tcmK, snoa1, aknB, mtmP; minimal PKS ketosynthase (KS/KS alpha)
09180 Brite Hierarchies
  09181 Protein families: metabolism
   01004 Lipid biosynthesis proteins
    K05551  actI1, oxyA, tcmK, snoa1, aknB, mtmP; minimal PKS ketosynthase (KS/KS alpha)
   01008 Polyketide biosynthesis proteins
    K05551  actI1, oxyA, tcmK, snoa1, aknB, mtmP; minimal PKS ketosynthase (KS/KS alpha)
Enzymes [BR:ko01000]
2. Transferases
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.235  tetracenomycin F2 synthase
     K05551  actI1, oxyA, tcmK, snoa1, aknB, mtmP; minimal PKS ketosynthase (KS/KS alpha)
    2.3.1.260  tetracycline polyketide synthase
     K05551  actI1, oxyA, tcmK, snoa1, aknB, mtmP; minimal PKS ketosynthase (KS/KS alpha)
Lipid biosynthesis proteins [BR:ko01004]
Polyketide synthase
  Component type
   K05551  actI1, oxyA, tcmK, snoa1, aknB, mtmP; minimal PKS ketosynthase (KS/KS alpha)
Polyketide biosynthesis proteins [BR:ko01008]
Polyketide synthase (PKS)
  Type II PKS
   C7-C14 cyclized polyketide type
    Octaketide PKS
     K05551  actI1, oxyA, tcmK, snoa1, aknB, mtmP; minimal PKS ketosynthase (KS/KS alpha)
    Nonaketide PKS
     K05551  actI1, oxyA, tcmK, snoa1, aknB, mtmP; minimal PKS ketosynthase (KS/KS alpha)
    Decaketide PKS
     K05551  actI1, oxyA, tcmK, snoa1, aknB, mtmP; minimal PKS ketosynthase (KS/KS alpha)

Genes

MBD:	MEBOL_003040

BALU:

QRY64_01340

BHUI:

LOK74_08075

PDY:	QJQ58_17940

PAUN:

MJA45_09960

BWX:	NQ550_10275

MSAO:

MYCSP_12700

MSAL:

DSM43276_02543

NBR:	O3I_007445 O3I_015110

NNO:	NONO_c10810

NTP:	CRH09_08670

NOZ:	DMB37_03470

NAH:	F5544_10735 F5544_17020 F5544_31065

NIE:	KV110_14170

NTC:	KHQ06_30035

NYA:	LTV02_09610

NGP:	LTT66_26675

NSPU:

IFM12276_11100

SCO:	SCO5087(actIORF1)

SMA:	SAVERM_2376(pks9-4)

SFI:

SFUL_4002

SANL:

KZO11_29480 KZO11_31415

SGRF:

SGFS_016120

SCYE:

R2B67_24745

SSX:	SACTE_5202 SACTE_6346

SHY:

SHJG_1189

SHO:	SHJGH_1024

SFA:

Sfla_5318

SBH:

SBI_06844

SVE:

SVEN_5977

SDV:	BN159_2471 BN159_2515(tcmK)

SALS:

SLNWT_5121

STRP:

F750_1305(whiE-KS)

SCI:	B446_13320

SLV:	SLIV_12950

SGU:	SGLAU_26355(tcmK) SGLAU_28980

SVT:	SVTN_18780 SVTN_28595

STRE:

GZL_p00214

SCW:	TU94_31825

SLD:	T261_0018 T261_6830

SXI:	SXIM_35610

STRC:

AA958_14835 AA958_30255

SAMB:

SAM23877_0196(alpA) SAM23877_0208(alpR) SAM23877_7465(alpR) SAM23877_7477(alpA)

SPRI:

SPRI_0266 SPRI_0280 SPRI_7073 SPRI_7087

SCZ:	ABE83_16245

SCX:	AS200_01095

SRW:	TUE45_01297 TUE45_01570

STRF:

ASR50_01175 ASR50_34265

SPAV:

Spa2297_32720

SGS:	AVL59_46045

SGM:	GCM10017557_73750(fabF)

SPHW:

NFX46_12170 NFX46_24985

SLS:	SLINC_6895

SALU:

DC74_6590

SALL:

SAZ_34270

STRD:

NI25_18955

SVU:	B1H20_09640

SPUN:

BFF78_19350

SLAU:

SLA_3074

SALJ:

SMD11_0675(actI1) SMD11_6044(actI1)

SLX:	SLAV_40245 SLAV_40510

SNZ:	DC008_13180

SGE:	DWG14_07360

SLK:	SLUN_33540

SDX:	C4B68_09595

SGD:	ELQ87_00815 ELQ87_08170

SCYA:

EJ357_29965 EJ357_34780

SNQ:	CP978_29750

STIR:

DDW44_27780

SKA:	CP970_02740 CP970_39675

SGZ:	C0216_31850

SNK:	CP967_10260

SHAW:

CEB94_23570

SRK:	FGW37_04255 FGW37_16265

SFIC:

EIZ62_03850

SGAL:

CP966_29070

SSPO:

DDQ41_07350

SVR:	CP971_23915

SFY:	GFH48_20455

SAQU:

EJC51_31355

SGF:	HEP81_07943

SCAV:

CVT27_18210

SRIM:

CP984_02375

SCIN:

CP977_28055

SPAC:

B1H29_00525 B1H29_36535

STSU:

B7R87_22035

SCHA:

CP983_03650 CP983_27885

SGX:	H4W23_11750

SCOE:

CP976_32915

SBAT:

G4Z16_23870

SSPB:

CP982_19005

SNF:	JYK04_04012

SPLA:

CP981_11520 CP981_34530

STUI:

GCM10017668_38110(fabF)

SCHF:

SHUN:

SHAR:

SCYG:

SFEU:

SLIA:

SDW:	K7C20_32760

SCAL:

SAUH:

SMOB:

SPEU:

SROI:

IAG44_01160 IAG44_09615 IAG44_37955 IAG44_41430

SXN:	IAG42_35940 IAG42_36715

SHK:	J2N69_28355

SANU:

K7396_04310

SDD:	D9753_08525

SACT:

SMAO:

SGOB:

SINE:

SLF:	JEQ17_32810

SDEC:

L3078_20575

SYAN:

NRK68_34880

STAA:

LDH80_09585

SNIG:

HEK616_47550(fabF_4)

SSPN:

LXH13_13940

SCIR:

STRCI_006078 STRCI_006121

SLON:

SDRZ:

STUD:

SARG:

SJN:	RI060_18280

SCYN:

N8I84_12665

SOV:	QZH56_16790 QZH56_23785

SHAU:

K9S39_40675

SKG:	KJK29_16075

SLAI:

SROC:

STTN:

SINN:

KBU:	Q4V64_27180

SARM:

DVA86_07645

STPB:

QR97_20400 QR97_38780

SRPA:

HEP86_12725

SMIB:

SMIR_18360

SCAJ:

O1G22_43255

KAB:	B7C62_31265

KAU:	B6264_18490

KIT:	CFP65_4148

KIS:	HUT16_23685

KCH:	O1G21_22135

YIA:	LO772_34320

STRH:

GXP74_03145

ABRY:

NYE86_15470 NYE86_15935

AGLA:

LMOI:

TFU:

TALX:

TCU:

AGRA:

ACIR:

OG979_01955 OG979_20970 OG979_36250

SPIQ:

OHA34_06345

SRO:

Sros_4150

NOA:	BKM31_22830

NCX:	Nocox_12685

FRA:	Francci3_4101

FRI:	FraEuI1c_1317

SVI:	Svir_33690

AMD:	AMED_5435(fabF)

AMN:

RAM_27705

AMM:	AMES_5370(fabF)

AMZ:	B737_5370(fabF)

AORI:

SD37_09325 SD37_34200

APRT:

ACOR:

PSEQ:

PECQ:

PHH:	AFB00_31125

PSDS:

K1T35_37455

SESP:

BN6_54860

SSYI:

EKG83_16320 EKG83_32220

SACX:

IOD16_13125

KAL:

KALB_7831

KUT:	JJ691_15650

KPHY:

AOZ06_32115

LED:	BBK82_02455

AHM:	TL08_10465

ACTI:

UA75_11090

ACAD:

UA74_11000

AHG:	AHOG_10345

ACTA:

ALO:

PMAD:

ACTU:

CROS:

STP:	Strop_2223

MAU:	Micau_4019

MIL:

ML5_4402

MICH:

FJK98_03840

MSAG:

GCM10017556_44990(fabF)

VMA:	VAB18032_18175

VER:	HUT12_05340

MFEU:

H1D33_25700

MCAY:

PVK37_16760 PVK37_24380

MICF:

QQG74_18960

AMS:	AMIS_50430 AMIS_50640

ACTN:

L083_3109

AIH:	Aiant_86560(fabF_2)

ACTR:

Asp14428_76450(fabF_2)

ACTL:

L3i22_085310

PLK:	CIK06_11005

CATI:

CS0771_73180(fabF_1)

DVC:	Dvina_17880

DFU:	Dfulv_18390

DROS:

Drose_14695 Drose_24335

DAUR:

Daura_28650 Daura_37850

DACT:

KZZ52_34430

NHY:	JQS43_06235

CCAZ:

COUCH_16055

CAI:	Caci_3546 Caci_6485

KBB:	ccbrp13_66830

BMEI:

Spa11_38550

AG:	AAA65206(dpsA) AAF70106(aknB) AAG30189(zhuB) AAZ78325(oxyA) CAA12017(snoa1) CAA61989(mtmP)

» show all

Reference

PMID:1527048

Authors

Fernandez-Moreno MA, Martinez E, Boto L, Hopwood DA, Malpartida F

Title

Nucleotide sequence and deduced functions of a set of cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin.

Journal

J Biol Chem 267:19278-90 (1992)

Sequence

[sco:SCO5087]

Reference

PMID:16597959

Authors

Zhang W, Ames BD, Tsai SC, Tang Y

Title

Engineered biosynthesis of a novel amidated polyketide, using the malonamyl-specific initiation module from the oxytetracycline polyketide synthase.

Journal

Appl Environ Microbiol 72:2573-80 (2006)
DOI:10.1128/AEM.72.4.2573-2580.2006

Sequence

[ag:AAZ78325]

Reference

PMID:20522541

Authors

Pickens LB, Tang Y

Title

Oxytetracycline biosynthesis.

Journal

J Biol Chem 285:27509-15 (2010)
DOI:10.1074/jbc.R110.130419

Reference

PMID:2684656

Authors

Bibb MJ, Biro S, Motamedi H, Collins JF, Hutchinson CR

Title

Analysis of the nucleotide sequence of the Streptomyces glaucescens tcmI genes provides key information about the enzymology of polyketide antibiotic biosynthesis.

Journal

EMBO J 8:2727-36 (1989)
DOI:10.1002/j.1460-2075.1989.tb08414.x

Sequence

[sgu:SGLAU_26355]

Reference

PMID:8668120

Authors

Ylihonko K, Tuikkanen J, Jussila S, Cong L, Mantsala P

Title

A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway.

Journal

Mol Gen Genet 251:113-20 (1996)
DOI:10.1007/BF02172908

Sequence

[ag:CAA12017]

Reference

PMID:8654997

Authors

Lombo F, Blanco G, Fernandez E, Mendez C, Salas JA

Title

Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin.

Journal

Gene 172:87-91 (1996)
DOI:10.1016/0378-1119(96)00029-7

Sequence

[ag:CAA61989]

LinkDB

ORTHOLOGY: K05552

Entry

K05552                      KO

Symbol

actI2, oxyB, tcmL, snoa2, aknC, mtmK

Name

minimal PKS chain-length factor (CLF/KS beta) [EC:2.3.1.- 2.3.1.260 2.3.1.235]

Pathway

map00253

Tetracycline biosynthesis

map01056

Biosynthesis of type II polyketide backbone

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Module

M00778

Type II polyketide backbone biosynthesis, acyl-CoA + malonyl-CoA => polyketide

Reaction

R06635

R06637

R06641

R06643

R06644

R06645

R09258

R09259

R10960

malonyl-CoA:acetate malonyltransferase (tetracenomycin-F2-forming)

R11516

malonyl-CoA:malonamoyl-[acyl-carrier protein] malonyltransferase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01056 Biosynthesis of type II polyketide backbone
    K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
   00253 Tetracycline biosynthesis
    K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
09180 Brite Hierarchies
  09181 Protein families: metabolism
   01004 Lipid biosynthesis proteins
    K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
   01008 Polyketide biosynthesis proteins
    K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
Enzymes [BR:ko01000]
2. Transferases
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.235  tetracenomycin F2 synthase
     K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
    2.3.1.260  tetracycline polyketide synthase
     K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
Lipid biosynthesis proteins [BR:ko01004]
Polyketide synthase
  Component type
   K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
Polyketide biosynthesis proteins [BR:ko01008]
Polyketide synthase (PKS)
  Type II PKS
   C7-C14 cyclized polyketide type
    Octaketide PKS
     K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
    Nonaketide PKS
     K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
    Decaketide PKS
     K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)

Genes

MBD:	MEBOL_003041

MSAO:

MYCSP_12695

MSAL:

DSM43276_02542

NBR:	O3I_007450 O3I_015115

NNO:	NONO_c10800

NTP:	CRH09_08675

NOZ:	DMB37_03465

NAH:	F5544_10730 F5544_17025 F5544_31060

NIE:	KV110_14175

NTC:	KHQ06_30030

NYA:	LTV02_09615

NGP:	LTT66_26670

NSPU:

IFM12276_11090

SCO:	SCO5088(actIORF2)

SMA:	SAVERM_2375(pks9-3)

SFI:

SANL:

SGRF:

SCYE:

SSX:	SACTE_5203 SACTE_6347

SHY:

SHJG_1188

SHO:	SHJGH_1023

SFA:

Sfla_5317

SBH:

SBI_06845

SVE:

SVEN_5981

SDV:	BN159_2470 BN159_2514(tcmL)

SALS:

SLNWT_5122

STRP:

F750_1306(whiE-CLF)

SCI:	B446_13325

SLV:	SLIV_12945

SGU:	SGLAU_26360(tcmL) SGLAU_28975

SVT:	SVTN_18775 SVTN_28600

STRE:

GZL_p00215

SCW:	TU94_31830

SLD:	T261_0019 T261_6831

SXI:	SXIM_35600

STRM:

M444_36340

STRC:

AA958_14840 AA958_30260

SAMB:

SAM23877_0195(alpB) SAM23877_0207(alpQ) SAM23877_7466(alpQ) SAM23877_7478(alpB)

SPRI:

SPRI_0267 SPRI_0278 SPRI_7075 SPRI_7086

SCZ:	ABE83_16240

SCX:	AS200_01090

SRW:	TUE45_01296 TUE45_01569

STRF:

ASR50_01170 ASR50_34270

SPAV:

Spa2297_32725

SGS:	AVL59_46040

SGM:	GCM10017557_73760

SPHW:

NFX46_12165 NFX46_24980

SLS:	SLINC_6894

SALU:

DC74_6591

SALL:

SAZ_34275

STRD:

NI25_18960

SVU:	B1H20_09645

SPUN:

BFF78_19355

SLAU:

SLA_3073

SALJ:

SMD11_0676(actI2) SMD11_1144(actI2) SMD11_6045(actI2)

SLX:	SLAV_40240 SLAV_40515

SNZ:	DC008_13175

SGE:	DWG14_07359

SLK:	SLUN_33545

SDX:	C4B68_09600

SGD:	ELQ87_00820 ELQ87_08175

SCYA:

EJ357_29970 EJ357_34775

SNQ:	CP978_29745

STIR:

DDW44_27775

SKA:	CP970_02735 CP970_39680

SGZ:	C0216_31855

SNK:	CP967_10255

SHAW:

CEB94_23575

SRK:	FGW37_04260 FGW37_16270

SFIC:

EIZ62_03845

SGAL:

CP966_29075

SSPO:

DDQ41_07355

SVR:	CP971_23920

SFY:	GFH48_20450

SAQU:

EJC51_31360

SGF:	HEP81_07942

SCAV:

CVT27_18215

SRIM:

CP984_02380

SCIN:

CP977_28050

SPAC:

B1H29_00520 B1H29_36540

STSU:

B7R87_22040

SCHA:

CP983_03645 CP983_27890

SGX:	H4W23_11745

SCOE:

CP976_32910

SBAT:

G4Z16_23875

SSPB:

CP982_19000

SNF:	JYK04_04013

SPLA:

CP981_11515 CP981_34525

STUI:

SCHF:

SHUN:

SHAR:

SCYG:

SFEU:

SLIA:

SDW:	K7C20_32765

SCAL:

SAUH:

SMOB:

SPEU:

SROI:

IAG44_09610 IAG44_37960 IAG44_41435

SXN:	IAG42_35945 IAG42_36720

SHK:	J2N69_28360

SANU:

K7396_04305

SDD:	D9753_08530

SACT:

SMAO:

SGOB:

SINE:

SLF:	JEQ17_32815

SDEC:

SDUR:

SYAN:

STAA:

SNIG:

SSPN:

SCIR:

STRCI_006079 STRCI_006122

SLON:

LGI35_31855

SDRZ:

NEH16_30130 NEH16_32490

STUD:

STRTU_005095

SGK:	PET44_33780

SARG:

HKX69_34110

SJN:	RI060_18275

SCYN:

N8I84_12670

SOV:	QZH56_16780 QZH56_23790

SHAU:

K9S39_40680

SKG:	KJK29_16070

STTN:

BSL84_24940

SINN:

ABB07_18370

KBU:	Q4V64_27185

SARM:

DVA86_07640

STPB:

QR97_20405 QR97_38785

SRPA:

HEP86_12720

SMIB:

SMIR_18355

SCAJ:

O1G22_43260

KAB:	B7C62_31270

KAU:	B6264_18495

KIT:	CFP65_4149

KIS:	HUT16_23690

KCH:	O1G21_22140

YIA:	LO772_34315

STRH:

GXP74_03140

ABRY:

NYE86_15465 NYE86_15940

AGLA:

LMOI:

TFU:

TALX:

NAKE:

TCU:

AGRA:

ACIR:

OG979_01950 OG979_20965 OG979_36255

SPIQ:

OHA34_06340

SRO:

Sros_4151

NOA:	BKM31_22870

NCX:	Nocox_12790

FRA:	Francci3_4100 Francci3_4148

FRI:	FraEuI1c_1318

SVI:	Svir_33700

AMD:	AMED_5434(fabF)

AMN:

RAM_27700

AMM:	AMES_5369(fabF)

AMZ:	B737_5369(fabF)

AORI:

SD37_09330 SD37_34300

APRT:

ACYN:

ACOR:

PSEQ:

PECQ:

PHH:	AFB00_31130

PSDS:

K1T35_37460

SESP:

BN6_54870

SSYI:

EKG83_16315 EKG83_32215

KAL:

KALB_7830

KUT:	JJ691_15660

KPHY:

AOZ06_32110

LED:	BBK82_02450

AHM:	TL08_10470

ACTI:

UA75_11095

ACAD:

UA74_11005

AHG:	AHOG_10350

ACTA:

ALO:

PMAD:

ACTU:

CROS:

STP:	Strop_2224

MAU:	Micau_4018

MIL:

ML5_4403

MICH:

FJK98_03845

MSAG:

GCM10017556_44980

VMA:	VAB18032_18170

VER:	HUT12_05335

MENO:

Jiend_01490

MCHL:

PVK74_07695

MFEU:

H1D33_23035 H1D33_25695

MHAW:

RMN56_24400

MCAY:

PVK37_16755 PVK37_21255 PVK37_24375

MICF:

QQG74_18955

AMS:	AMIS_50420 AMIS_50650

ACTN:

L083_3108

AIH:	Aiant_86570(fabF_3)

ACTR:

Asp14428_76460(fabF_3)

ACTL:

L3i22_085320

PLK:	CIK06_11010

CATI:

CS0771_73190

DVC:	Dvina_17885

DFU:	Dfulv_18395

DROS:

Drose_14745 Drose_24330

DAUR:

Daura_28645 Daura_37855

DACT:

KZZ52_34425

NHY:	JQS43_06230

CCAZ:

COUCH_16060

CAI:	Caci_3547 Caci_6486

KBB:	ccbrp13_66820(fabF_2)

AG:	AAA65207(dpsB) AAF70107(aknC) AAG30188(zhuA) AAZ78326(oxyB) CAA12018(snoa2) CAA61990(mtmK)

» show all

Reference

PMID:1527048

Authors

Fernandez-Moreno MA, Martinez E, Boto L, Hopwood DA, Malpartida F

Title

Nucleotide sequence and deduced functions of a set of cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin.

Journal

J Biol Chem 267:19278-90 (1992)

Sequence

[sco:SCO5088]

Reference

PMID:16597959

Authors

Zhang W, Ames BD, Tsai SC, Tang Y

Title

Engineered biosynthesis of a novel amidated polyketide, using the malonamyl-specific initiation module from the oxytetracycline polyketide synthase.

Journal

Appl Environ Microbiol 72:2573-80 (2006)
DOI:10.1128/AEM.72.4.2573-2580.2006

Sequence

[ag:AAZ78326]

Reference

PMID:20522541

Authors

Pickens LB, Tang Y

Title

Oxytetracycline biosynthesis.

Journal

J Biol Chem 285:27509-15 (2010)
DOI:10.1074/jbc.R110.130419

Reference

PMID:2684656

Authors

Bibb MJ, Biro S, Motamedi H, Collins JF, Hutchinson CR

Title

Analysis of the nucleotide sequence of the Streptomyces glaucescens tcmI genes provides key information about the enzymology of polyketide antibiotic biosynthesis.

Journal

EMBO J 8:2727-36 (1989)
DOI:10.1002/j.1460-2075.1989.tb08414.x

Sequence

[sgu:SGLAU_26360]

Reference

PMID:8668120

Authors

Ylihonko K, Tuikkanen J, Jussila S, Cong L, Mantsala P

Title

A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway.

Journal

Mol Gen Genet 251:113-20 (1996)
DOI:10.1007/BF02172908

Sequence

[ag:CAA12018]

Reference

PMID:8654997

Authors

Lombo F, Blanco G, Fernandez E, Mendez C, Salas JA

Title

Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin.

Journal

Gene 172:87-91 (1996)
DOI:10.1016/0378-1119(96)00029-7

Sequence

[ag:CAA61990]

LinkDB

ORTHOLOGY: K05553

Entry

K05553                      KO

Symbol

actI3, oxyC, tcmM, snoa3, aknD, mtmS

Name

minimal PKS acyl carrier protein

Pathway

map00253

Tetracycline biosynthesis

map01056

Biosynthesis of type II polyketide backbone

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Module

M00778

Type II polyketide backbone biosynthesis, acyl-CoA + malonyl-CoA => polyketide

Reaction

R06635

R06637

R06641

R06643

R06644

R06645

R09258

R09259

R10960

malonyl-CoA:acetate malonyltransferase (tetracenomycin-F2-forming)

R11516

malonyl-CoA:malonamoyl-[acyl-carrier protein] malonyltransferase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01056 Biosynthesis of type II polyketide backbone
    K05553  actI3, oxyC, tcmM, snoa3, aknD, mtmS; minimal PKS acyl carrier protein
   00253 Tetracycline biosynthesis
    K05553  actI3, oxyC, tcmM, snoa3, aknD, mtmS; minimal PKS acyl carrier protein
09180 Brite Hierarchies
  09181 Protein families: metabolism
   01004 Lipid biosynthesis proteins
    K05553  actI3, oxyC, tcmM, snoa3, aknD, mtmS; minimal PKS acyl carrier protein
   01008 Polyketide biosynthesis proteins
    K05553  actI3, oxyC, tcmM, snoa3, aknD, mtmS; minimal PKS acyl carrier protein
Lipid biosynthesis proteins [BR:ko01004]
Polyketide synthase
  Component type
   K05553  actI3, oxyC, tcmM, snoa3, aknD, mtmS; minimal PKS acyl carrier protein
Polyketide biosynthesis proteins [BR:ko01008]
Polyketide synthase (PKS)
  Type II PKS
   C7-C14 cyclized polyketide type
    Octaketide PKS
     K05553  actI3, oxyC, tcmM, snoa3, aknD, mtmS; minimal PKS acyl carrier protein
    Nonaketide PKS
     K05553  actI3, oxyC, tcmM, snoa3, aknD, mtmS; minimal PKS acyl carrier protein
    Decaketide PKS
     K05553  actI3, oxyC, tcmM, snoa3, aknD, mtmS; minimal PKS acyl carrier protein

Genes

MBD:	MEBOL_003042

MSAO:

MYCSP_12690

MSAL:

DSM43276_02541

CCAW:

CCANI_10135

NBR:	O3I_007455 O3I_015120

NNO:	NONO_c10790

NTP:	CRH09_08680

NOZ:	DMB37_03460

NAH:	F5544_10725 F5544_17030 F5544_31055

NAD:	NCTC11293_03460

NWL:	NWFMUON74_57140

NIE:	KV110_14180

NTC:	KHQ06_30025

NYA:	LTV02_09620

NGP:	LTT66_26665

NSPU:

IFM12276_11080

SCO:	SCO1272(2SCG18.19c) SCO5089(actIORF3)

SMA:	SAVERM_2373(pks9-1)

SFI:

SANL:

SGRF:

SCYE:

SSX:	SACTE_5205 SACTE_6348

SHY:

SHJG_1187

SHO:	SHJGH_1022

SFA:

Sfla_5316

SBH:

SBI_06846

SVE:

SVEN_5982

SDV:	BN159_2492 BN159_2513

SALS:

SLNWT_5123

STRP:

F750_1307

SCI:	B446_13330

SLV:	SLIV_12940 SLIV_31430

SGU:	SGLAU_26365(tcmM)

SVT:	SVTN_18770 SVTN_28605

STRE:

GZL_p00216

SCW:	TU94_31835

SLD:	T261_0020 T261_6832

SXI:	SXIM_35580 SXIM_35680

STRC:

AA958_14845 AA958_30265

SAMB:

SAM23877_0194(alpC) SAM23877_7479(alpC)

SPRI:

SPRI_0265 SPRI_0277 SPRI_7076 SPRI_7088

SCZ:	ABE83_16235

SCX:	AS200_01425

SRW:	TUE45_01110 TUE45_01317 TUE45_01568

STRF:

ASR50_01165 ASR50_34275

SPAV:

Spa2297_32730

SGS:	AVL59_46035

SGM:	GCM10017557_73770

SPHW:

NFX46_12160 NFX46_24975

SLS:	SLINC_6893

SALU:

DC74_6592

SALL:

SAZ_34280

STRD:

NI25_18965

SVU:	B1H20_09515

SPUN:

BFF78_19345

SLAU:

SLA_3072

SALJ:

SMD11_0677(actI3) SMD11_1145(actI3) SMD11_6046(actI3)

SLX:	SLAV_40235 SLAV_40285

SNZ:	DC008_13170

SGE:	DWG14_07358

SLK:	SLUN_33505

SDX:	C4B68_09605

SGD:	ELQ87_00825 ELQ87_08180

SCYA:

EJ357_29975 EJ357_34770

SNQ:	CP978_29740

STIR:

DDW44_27915

SKA:	CP970_02730 CP970_39685

SGZ:	C0216_31860

SNK:	CP967_10250

SHAW:

CEB94_23580

SRK:	FGW37_04265 FGW37_16275

SFIC:

EIZ62_03840

SGAL:

CP966_29080

SSPO:

DDQ41_07360

SVR:	CP971_23925

SFY:	GFH48_20445

SAQU:

EJC51_31365

SGF:	HEP81_07941

SCAV:

CVT27_18220

SRIM:

CP984_02385

SCIN:

CP977_27935

SPAC:

B1H29_00830 B1H29_36230

STSU:

B7R87_21965

SCHA:

CP983_03640 CP983_27900

SGX:	H4W23_11630 H4W23_11715

SCOE:

CP976_32955

SBAT:

G4Z16_23880

SSPB:

CP982_18995

SNF:	JYK04_04014 JYK04_04602

SPLA:

CP981_11510 CP981_34520

STUI:

SCHF:

SHUN:

SHAR:

SCYG:

SFEU:

SLIA:

SDW:	K7C20_32770

SCAL:

SAUH:

SMOB:

SPEU:

SROI:

IAG44_09650 IAG44_37975 IAG44_41440

SXN:	IAG42_35935 IAG42_36725

SHK:	J2N69_31110

SANU:

K7396_04300

SDD:	D9753_08535

SACT:

SMAO:

SGOB:

SINE:

SLF:	JEQ17_32800 JEQ17_32820

SDEC:

SYAN:

STAA:

SNIG:

SSPN:

SCIR:

STRCI_006080 STRCI_006102

SLON:

LGI35_31860

SDRZ:

NEH16_30125 NEH16_32485

STUD:

STRTU_005096

SYUN:

MOV08_33805

SARG:

HKX69_34115

SJN:	RI060_18410

SCYN:

N8I84_12675

SOV:	QZH56_16775 QZH56_23795

SHAU:

K9S39_40605

SKG:	KJK29_16210

SLAI:

SROC:

STTN:

SINN:

KBU:	Q4V64_27205

SARM:

DVA86_07635

STPB:

QR97_20410 QR97_28995 QR97_38685

SRPA:

SMIB:

SCAJ:

KSK:

KAB:	B7C62_31275

KAU:	B6264_18500

KIT:	CFP65_4142

KIS:	HUT16_23695

KCH:	O1G21_22145

YIA:	LO772_34325 LO772_34340

STRH:

GXP74_03135

ABRY:

NYE86_15460 NYE86_15945

LMOI:

VV02_05825

PACD:

EGX94_11640

TFU:

Tfu_1220

TALX:

FOF52_07525 FOF52_07535

TCU:

Tcur_2794

AGRA:

AGRA3207_004548

ACIR:

OG979_01945 OG979_36210

SPIQ:

OHA34_06575

SRO:

Sros_4152

NOA:	BKM31_22705

NCX:	Nocox_12785

NGN:	LCN96_42550

FRA:	Francci3_4099 Francci3_4147

FRI:	FraEuI1c_1319

SVI:	Svir_33710

AMD:	AMED_5433(actI)

AMN:

RAM_27695

AMM:	AMES_5368(actI)

AMZ:	B737_5368(actI)

AMYY:

YIM_23710

AORI:

SD37_09335 SD37_34250

APRT:

ACYN:

ACOR:

PSEQ:

PECQ:

PHH:	AFB00_31135

PSDS:

K1T35_37465

SESP:

BN6_54880(acp5)

SSYI:

EKG83_16310 EKG83_32210

KAL:

KALB_7829

KUT:	JJ691_15670

KPHY:

AOZ06_32105

LED:	BBK82_02475

AHM:	TL08_10475

ACTI:

UA75_11100

ACAD:

UA74_11010

AHG:	AHOG_10355

ACTA:

ALO:

PMAD:

ACTU:

CROS:

STP:	Strop_2225

MAU:	Micau_4017

MIL:

ML5_4404

MICH:

FJK98_03855

MSAG:

GCM10017556_44970

VMA:	VAB18032_18165

VER:	HUT12_05330

MENO:

Jiend_53200

MFEU:

H1D33_25690

MCAY:

PVK37_16750 PVK37_24370

AMS:	AMIS_50440

AIH:	Aiant_86580

ACTL:

L3i22_085330

PLK:	CIK06_11015

CATI:

CS0771_73140

DVC:	Dvina_17830 Dvina_17855

DFU:	Dfulv_18400

DROS:

Drose_14690 Drose_24325

DAUR:

Daura_28640 Daura_37920

NHY:	JQS43_06225

CCAZ:

COUCH_16065

CAI:	Caci_3548 Caci_6487

AG:	AAD04718(dpsG) AAF70108(aknD) AAG30201(zhuN) AAZ78327(oxyC) CAA12019(snoa3) CAA61991(mtmS)

» show all

Reference

PMID:1527048

Authors

Fernandez-Moreno MA, Martinez E, Boto L, Hopwood DA, Malpartida F

Title

Nucleotide sequence and deduced functions of a set of cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin.

Journal

J Biol Chem 267:19278-90 (1992)

Sequence

[sco:SCO5089]

Reference

PMID:16597959

Authors

Zhang W, Ames BD, Tsai SC, Tang Y

Title

Engineered biosynthesis of a novel amidated polyketide, using the malonamyl-specific initiation module from the oxytetracycline polyketide synthase.

Journal

Appl Environ Microbiol 72:2573-80 (2006)
DOI:10.1128/AEM.72.4.2573-2580.2006

Sequence

[ag:AAZ78327]

Reference

PMID:20522541

Authors

Pickens LB, Tang Y

Title

Oxytetracycline biosynthesis.

Journal

J Biol Chem 285:27509-15 (2010)
DOI:10.1074/jbc.R110.130419

Reference

PMID:2684656

Authors

Bibb MJ, Biro S, Motamedi H, Collins JF, Hutchinson CR

Title

Analysis of the nucleotide sequence of the Streptomyces glaucescens tcmI genes provides key information about the enzymology of polyketide antibiotic biosynthesis.

Journal

EMBO J 8:2727-36 (1989)
DOI:10.1002/j.1460-2075.1989.tb08414.x

Sequence

[sgu:SGLAU_26365]

Reference

PMID:1592832

Authors

Shen B, Summers RG, Gramajo H, Bibb MJ, Hutchinson CR

Title

Purification and characterization of the acyl carrier protein of the Streptomyces glaucescens tetracenomycin C polyketide synthase.

Journal

J Bacteriol 174:3818-21 (1992)
DOI:10.1128/JB.174.11.3818-3821.1992

Reference

PMID:8668120

Authors

Ylihonko K, Tuikkanen J, Jussila S, Cong L, Mantsala P

Title

A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway.

Journal

Mol Gen Genet 251:113-20 (1996)
DOI:10.1007/BF02172908

Sequence

[ag:CAA12019]

Reference

PMID:8654997

Authors

Lombo F, Blanco G, Fernandez E, Mendez C, Salas JA

Title

Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin.

Journal

Gene 172:87-91 (1996)
DOI:10.1016/0378-1119(96)00029-7

Sequence

[ag:CAA61991]

LinkDB

REACTION: R09259

Entry

R09259                      Reaction

Definition

3,5,7,9,11,13,15-Heptaoxo-hexadecanoyl-[acp] <=> 7,9,12-Octaketide intermediate 1

Equation

C12348 <=> C18327

Reaction class

RC02545

C12348_C18327

Pathway

rn01056

Biosynthesis of type II polyketide backbone

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

Module

M00778

Type II polyketide backbone biosynthesis, acyl-CoA + malonyl-CoA => polyketide

Orthology

K05551

minimal PKS ketosynthase (KS/KS alpha) [EC:2.3.1.- 2.3.1.260 2.3.1.235]

K05552

minimal PKS chain-length factor (CLF/KS beta) [EC:2.3.1.- 2.3.1.260 2.3.1.235]

K05553

minimal PKS acyl carrier protein

Reference

1 [PMID:15286722]

Authors

Keatinge-Clay AT, Maltby DA, Medzihradszky KF, Khosla C, Stroud RM

Title

An antibiotic factory caught in action.

Journal

Nat Struct Mol Biol 11:888-93 (2004)
DOI:10.1038/nsmb808

LinkDB

DBGET integrated database retrieval system