KEGG   ORTHOLOGY: K05552
Entry
K05552                      KO                                     
Symbol
actI2, oxyB, tcmL, snoa2, aknC, mtmK
Name
minimal PKS chain-length factor (CLF/KS beta) [EC:2.3.1.- 2.3.1.260 2.3.1.235]
Pathway
map00253  Tetracycline biosynthesis
map01056  Biosynthesis of type II polyketide backbone
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00778  Type II polyketide backbone biosynthesis, acyl-CoA + malonyl-CoA => polyketide
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01056 Biosynthesis of type II polyketide backbone
    K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
   00253 Tetracycline biosynthesis
    K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01004 Lipid biosynthesis proteins
    K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
   01008 Polyketide biosynthesis proteins
    K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
Enzymes [BR:ko01000]
 2. Transferases
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.235  tetracenomycin F2 synthase
     K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
    2.3.1.260  tetracycline polyketide synthase
     K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
    2.3.1.-
     K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
Lipid biosynthesis proteins [BR:ko01004]
 Polyketide synthase
  Component type
   K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide synthase (PKS)
  Type II PKS
   C7-C14 cyclized polyketide type
    Octaketide PKS
     K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
    Nonaketide PKS
     K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
    Decaketide PKS
     K05552  actI2, oxyB, tcmL, snoa2, aknC, mtmK; minimal PKS chain-length factor (CLF/KS beta)
Other DBs
RN: R06635 R06637 R06641 R06643 R06644 R06645 R09258 R09259 R10960 R11516
Genes
MBD: MEBOL_003041
MSAO: MYCSP_12695
MSAL: DSM43276_02542
NBR: O3I_007450 O3I_015115
NNO: NONO_c10800
NTP: CRH09_08675
NOZ: DMB37_03465
NAH: F5544_10730 F5544_17025 F5544_31060
NIE: KV110_14175
NTC: KHQ06_30030
SCO: SCO5088(actIORF2)
SMA: SAVERM_2375(pks9-3)
SHY: SHJG_1188
SFA: Sfla_5317
SBH: SBI_06845
SVE: SVEN_5981
SALS: SLNWT_5122
STRP: F750_1306(whiE-CLF)
SFI: SFUL_4003
SALU: DC74_6591
SALL: SAZ_34275
STRE: GZL_p00215
STRM: M444_36340
STRD: NI25_18960
SLAU: SLA_3073
SALJ: SMD11_0676(actI2) SMD11_1144(actI2) SMD11_6045(actI2)
SAUH: SU9_027640
LMOI: VV02_05820
TFU: Tfu_1219
TCU: Tcur_2772
SRO: Sros_4151
AMD: AMED_5434(fabF)
AMN: RAM_27700
AMM: AMES_5369(fabF)
AMZ: B737_5369(fabF)
SESP: BN6_54870
KAL: KALB_7830
ACTI: UA75_11095
ACAD: UA74_11005
ALO: CRK55092
MIL: ML5_4403
ACTN: L083_3108
AIH: Aiant_86570(fabF_3)
ACTR: Asp14428_76460(fabF_3)
AG: AAA65207(dpsB) AAF70107(aknC) AAG30188(zhuA) AAZ78326(oxyB) CAA12018(snoa2) CAA61990(mtmK)
 » show all
Reference
PMID:1527048
  Authors
Fernandez-Moreno MA, Martinez E, Boto L, Hopwood DA, Malpartida F
  Title
Nucleotide sequence and deduced functions of a set of cotranscribed genes of Streptomyces coelicolor A3(2) including the polyketide synthase for the antibiotic actinorhodin.
  Journal
J Biol Chem 267:19278-90 (1992)
  Sequence
[sco:SCO5088]
Reference
  Authors
Zhang W, Ames BD, Tsai SC, Tang Y
  Title
Engineered biosynthesis of a novel amidated polyketide, using the malonamyl-specific initiation module from the oxytetracycline polyketide synthase.
  Journal
Appl Environ Microbiol 72:2573-80 (2006)
DOI:10.1128/AEM.72.4.2573-2580.2006
  Sequence
Reference
  Authors
Pickens LB, Tang Y
  Title
Oxytetracycline biosynthesis.
  Journal
J Biol Chem 285:27509-15 (2010)
DOI:10.1074/jbc.R110.130419
Reference
PMID:2684656
  Authors
Bibb MJ, Biro S, Motamedi H, Collins JF, Hutchinson CR
  Title
Analysis of the nucleotide sequence of the Streptomyces glaucescens tcmI genes provides key information about the enzymology of polyketide antibiotic biosynthesis.
  Journal
EMBO J 8:2727-36 (1989)
DOI:10.1002/j.1460-2075.1989.tb08414.x
  Sequence
Reference
PMID:8668120
  Authors
Ylihonko K, Tuikkanen J, Jussila S, Cong L, Mantsala P
  Title
A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway.
  Journal
Mol Gen Genet 251:113-20 (1996)
DOI:10.1007/BF02172908
  Sequence
Reference
PMID:8654997
  Authors
Lombo F, Blanco G, Fernandez E, Mendez C, Salas JA
  Title
Characterization of Streptomyces argillaceus genes encoding a polyketide synthase involved in the biosynthesis of the antitumor mithramycin.
  Journal
Gene 172:87-91 (1996)
DOI:10.1016/0378-1119(96)00029-7
  Sequence
LinkDB

KEGG   REACTION: R09258
Entry
R09258                      Reaction                               
Definition
8 Malonyl-CoA + Acyl-carrier protein <=> 3,5,7,9,11,13,15-Heptaoxo-hexadecanoyl-[acp] + 8 CO2 + 8 CoA
Equation
8 C00083 + C00229 <=> C12348 + 8 C00011 + 8 C00010
Comment
minimal PKS, actIORF1+actIORF2+actIORF3
Reaction class
RC00004  C00010_C00083
RC00039  C00229_C12348
RC02931  C00083_C12348
RC02932  C00083_C12348
RC02947  C00083_C12348
Enzyme
2.3.1.-
Pathway
rn01056  Biosynthesis of type II polyketide backbone
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00778  Type II polyketide backbone biosynthesis, acyl-CoA + malonyl-CoA => polyketide
Orthology
K05551  minimal PKS ketosynthase (KS/KS alpha) [EC:2.3.1.- 2.3.1.260 2.3.1.235]
K05552  minimal PKS chain-length factor (CLF/KS beta) [EC:2.3.1.- 2.3.1.260 2.3.1.235]
K05553  minimal PKS acyl carrier protein
LinkDB

KEGG   REACTION: R06635
Entry
R06635                      Reaction                               
Definition
Acetyl-CoA + 7 Malonyl-CoA + Acyl-carrier protein <=> 3,5,7,9,11,13,15-Heptaoxo-hexadecanoyl-[acp] + 7 CO2 + 7 CoA
Equation
C00024 + 7 C00083 + C00229 <=> C12348 + 7 C00011 + 7 C00010
Reaction class
RC00004  C00010_C00024  C00010_C00083
RC02729  C00024_C12348
RC02931  C00083_C12348
RC02932  C00083_C12348
Enzyme
2.3.1.-
Pathway
rn01056  Biosynthesis of type II polyketide backbone
Orthology
K05551  minimal PKS ketosynthase (KS/KS alpha) [EC:2.3.1.- 2.3.1.260 2.3.1.235]
K05552  minimal PKS chain-length factor (CLF/KS beta) [EC:2.3.1.- 2.3.1.260 2.3.1.235]
K05553  minimal PKS acyl carrier protein
LinkDB

KEGG   REACTION: R06637
Entry
R06637                      Reaction                               
Definition
Butanoyl-CoA + 7 Malonyl-CoA + Acyl-carrier protein <=> 3,5,7,9,11,13,15-Heptaoxo-octadecanoyl-[acp] + 7 CO2 + 7 CoA
Equation
C00136 + 7 C00083 + C00229 <=> C12350 + 7 C00011 + 7 C00010
Comment
minimal PKS
Reaction class
RC00004  C00010_C00083  C00010_C00136
RC00039  C00229_C12350
RC02728  C00136_C12350
RC02931  C00083_C12350
RC02932  C00083_C12350
Enzyme
2.3.1.-
Pathway
rn01056  Biosynthesis of type II polyketide backbone
Orthology
K05551  minimal PKS ketosynthase (KS/KS alpha) [EC:2.3.1.- 2.3.1.260 2.3.1.235]
K05552  minimal PKS chain-length factor (CLF/KS beta) [EC:2.3.1.- 2.3.1.260 2.3.1.235]
K05553  minimal PKS acyl carrier protein
LinkDB

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