KEGG   ORTHOLOGY: K07436
Entry
K07436                      KO                                     
Symbol
CYP24A1
Name
vitamin D3 24-hydroxylase [EC:1.14.15.16]
Pathway
map00100  Steroid biosynthesis
map01100  Metabolic pathways
map04928  Parathyroid hormone synthesis, secretion and action
map05206  MicroRNAs in cancer
Disease
H01371  Hypercalcemia infantile
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09103 Lipid metabolism
   00100 Steroid biosynthesis
    K07436  CYP24A1; vitamin D3 24-hydroxylase
 09150 Organismal Systems
  09152 Endocrine system
   04928 Parathyroid hormone synthesis, secretion and action
    K07436  CYP24A1; vitamin D3 24-hydroxylase
 09160 Human Diseases
  09161 Cancer: overview
   05206 MicroRNAs in cancer
    K07436  CYP24A1; vitamin D3 24-hydroxylase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K07436  CYP24A1; vitamin D3 24-hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.15  With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.15.16  vitamin D3 24-hydroxylase
     K07436  CYP24A1; vitamin D3 24-hydroxylase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, animal type
  CYP24 family
   K07436  CYP24A1; vitamin D3 24-hydroxylase
Other DBs
RN: R09515 R09516
GO: 0008403 0030342
Genes
HSA: 1591(CYP24A1)
PTR: 746729(CYP24A1)
PPS: 100971976(CYP24A1)
GGO: 101142284
PON: 100457433
NLE: 100603459
MCC: 698937
MCF: 102117231(CYP24A1)
MTHB: 126929847
CSAB: 103243965(CYP24A1)
CATY: 105585362
PANU: 100997369
TGE: 112632353
RRO: 104662355
RBB: 108521781
TFN: 117068948
PTEH: 111521023
CJC: 100393283
SBQ: 101054511
CSYR: 103265086
LCAT: 123622312
OGA: 100948814
MMU: 13081(Cyp24a1)
MCAL: 110289544
MPAH: 110318266
RNO: 25279(Cyp24a1)
MCOC: 116091462
MUN: 110545262
CGE: 100751280
MAUA: 101827330
PLEU: 114704035
MORG: 121440893
MFOT: 126508008
AAMP: 119815492
NGI: 103735781
HGL: 101716773
CPOC: 100728021
CCAN: 109694681
DORD: 105991653
DSP: 122124591
NCAR: 124978047
OCU: 100347441
OPI: 101526808
TUP: 102471055
CFA: 485935(CYP24A1)
CLUD: 112674140
VVP: 112931920
VLG: 121477768
AML: 100479790
UMR: 103669826
UAH: 113258741
UAR: 123799375
ELK: 111141789
LLV: 125109083
MPUF: 101678691
ORO: 101384402
EJU: 114225492
ZCA: 113938600
MLX: 118024098
NSU: 110569858
FCA: 101096141
PYU: 121019910
PBG: 122467253
LRUF: 124504391
PTG: 102967619
PPAD: 109272242
AJU: 106970319
HHV: 120230459
BTA: 540080(CYP24A1)
BOM: 102272075
BIU: 109567562
BBUB: 102396198
CHX: 102178921
OAS: 100170323(CYP24)
ODA: 120869048
CCAD: 122448475
SSC: 397145(CYP24A1)
CFR: 102516204
CBAI: 105071834
CDK: 105098859
VPC: 102531787
BACU: 103015403
LVE: 103082411
OOR: 101271151
DLE: 111186695
PCAD: 102977275
PSIU: 116740225
ECB: 100053452(CYP24A1)
EPZ: 103554239
EAI: 106825434
MYB: 102249295
MYD: 102772049
MMYO: 118663454
MLF: 102432347
MNA: 107536509
PKL: 118708044
HAI: 109381840
DRO: 112304205
SHON: 118996516
AJM: 119040651
PDIC: 114506963
PHAS: 123815183
MMF: 118619039
RFQ: 117015976
PALE: 102878689
PGIG: 120603843
PVP: 105296033
RAY: 107498143
MJV: 108390269
TOD: 119254587
SARA: 101543781
LAV: 100663262
TMU: 101353460
DNM: 101442213
MDO: 100028177
GAS: 123233554
SHR: 100916072
PCW: 110206681
OAA: 100078505
GGA: 395827(CYP24A1)
PCOC: 116227703
MGP: 100541378
CJO: 107323241
NMEL: 110408247
APLA: 101797037
ACYG: 106041795
AFUL: 116495811
TGU: 100226727
LSR: 110472394
SCAN: 103818886
PMOA: 120507065
OTC: 121342986
PRUF: 121359459
GFR: 102044237
FAB: 101813067
PHI: 102111029
PMAJ: 107213207
CCAE: 111938004
CCW: 104687601
CBRC: 103616823
ETL: 114056255
ZAB: 102067744
ACHL: 103801335
FPG: 101919538
FCH: 102046853
CLV: 102098516
EGZ: 104132191
NNI: 104013059
PLET: 104624707
PCRI: 104024392
ACUN: 113487633
TALA: 104355745
PADL: 103919278
AFOR: 103894637
ACHC: 115339853
HALD: 104317033
CCRI: 104163872
CSTI: 104559611
EHS: 104503318
CMAC: 104483098
MUI: 104537354
FGA: 104069789
GSTE: 104258977
LDI: 104340282
MNB: 103775032
OHA: 104328853
NNT: 104412533
DPUB: 104302691
PGUU: 104464606
ACAR: 104526380
AVIT: 104274781
CVF: 104293941
AAM: 106491745
AROW: 112960579
NPD: 112949674
TGT: 104576182
DNE: 112982887
SCAM: 104150031
ASN: 102382614
AMJ: 102574966(CYP24A1)
CPOO: 109318764
GGN: 109292377
PSS: 102458142
CMY: 102932138
CPIC: 101938071
TST: 117886274
CABI: 116821477
MRV: 120379784
PVT: 110071817
SUND: 121927742
PBI: 103065707
PMUR: 107287085
CTIG: 120308207
TSR: 106547483
PGUT: 117674844
VKO: 123036291
PMUA: 114599654
ZVI: 118089322
GJA: 107119104
STOW: 125432416
XLA: 108700906(cyp24a1.L) 108718538
XTR: 100496967 100497429(cyp24a1)
RTEM: 120940805
BBUF: 121001334
BGAR: 122939129
DRE: 100004700(cyp24a1)
SRX: 107744352
CCAR: 109057669
PPRM: 120493676
MAMB: 125273354
IPU: 108271496
PHYP: 113532087
SMEO: 124388872
TFD: 113642663
AMEX: 103030576
EEE: 113571660
TRU: 101077963
LCO: 104935382
NCC: 104966082
CGOB: 115008593
ELY: 117257164
EFO: 125895063
PLEP: 121959102
SLUC: 116051531
ECRA: 117943678
ESP: 116687595
PFLV: 114554700
GAT: 120835793
PPUG: 119194098
MSAM: 119885885
CUD: 121507546
ALAT: 119020664
MZE: 101475325
ONL: 100690876
OAU: 116319460
OLA: 101165632
OML: 112145619
XMA: 102225814
XCO: 114135962
XHE: 116711044
PRET: 103464673
PFOR: 103134567
PLAI: 106960246
PMEI: 106929021
GAF: 122833415
CVG: 107097105
CTUL: 119788661
GMU: 124856320
NFU: 107385407
KMR: 108231477
ALIM: 106533681
NWH: 119411875
AOCE: 111579624
MCEP: 125006146
CSEM: 103386736
POV: 109635373
SSEN: 122767772
HHIP: 117761120
HSP: 118103196
LCF: 108873562
SDU: 111233601
SLAL: 111660685
XGL: 120783454
HCQ: 109507475
BPEC: 110171174
MALB: 109951265
BSPL: 114856188
SASA: 106565076(CP24A) 106583685
ELS: 105016677
SFM: 108936451
PKI: 111835893
AANG: 118210803
LOC: 102698913
LCM: 102364004
CMK: 103191047
RTP: 109910917
HAME: 121870079
RSAN: 119392951
RMP: 119172898
XEN: 124454605
 » show all
Reference
PMID:8506296
  Authors
Chen KS, Prahl JM, DeLuca HF
  Title
Isolation and expression of human 1,25-dihydroxyvitamin D3 24-hydroxylase cDNA.
  Journal
Proc Natl Acad Sci U S A 90:4543-7 (1993)
DOI:10.1073/pnas.90.10.4543
  Sequence
[hsa:1591]
Reference
  Authors
Prosser DE, Jones G
  Title
Enzymes involved in the activation and inactivation of vitamin D.
  Journal
Trends Biochem Sci 29:664-73 (2004)
DOI:10.1016/j.tibs.2004.10.005
LinkDB

KEGG   ENZYME: 1.14.15.16
Entry
EC 1.14.15.16               Enzyme                                 
Name
vitamin D3 24-hydroxylase;
CYP24A1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
calcitriol,adrenodoxin:oxygen oxidoreductase (24-hydroxylating)
Reaction(IUBMB)
(1) calcitriol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitetrol + 2 oxidized adrenodoxin + H2O [RN:R09515];
(2) calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = secalciferol + 2 oxidized adrenodoxin + H2O [RN:R09516]
Reaction(KEGG)
R09515 R09516
Substrate
calcitriol [CPD:C01673];
reduced adrenodoxin [CPD:C00662];
H+ [CPD:C00080];
O2 [CPD:C00007];
calcidiol [CPD:C01561]
Product
calcitetrol [CPD:C18231];
oxidized adrenodoxin [CPD:C00667];
H2O [CPD:C00001];
secalciferol [CPD:C07712]
Comment
This mitochondrial cytochrome P-450 enzyme requires adrenodoxin. The enzyme can perform up to 6 rounds of hydroxylation of the substrate calcitriol leading to calcitroic acid. The human enzyme also shows 23-hydroxylating activity leading to 1,25 dihydroxyvitamin D3-26,23-lactone as end product while the mouse and rat enzymes do not. The initial source of the electrons is NADPH, which transfers the electrons to the adrenodoxin via EC 1.18.1.6, adrenodoxin-NADP+ reductase.
History
EC 1.14.15.16 created 2011 as EC 1.14.13.126, transferred 2016 to EC 1.14.15.16
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
Orthology
K07436  vitamin D3 24-hydroxylase
Genes
HSA1591(CYP24A1)
PTR746729(CYP24A1)
PPS100971976(CYP24A1)
GGO101142284
PON100457433
NLE100603459
MCC698937
MCF102117231(CYP24A1)
MTHB126929847
CSAB103243965(CYP24A1)
CATY105585362
PANU100997369
TGE112632353
RRO104662355
RBB108521781
TFN117068948
PTEH111521023
CJC100393283
SBQ101054511
CSYR103265086
MMUR105864362 105869419
LCAT123622312
OGA100948814
MMU13081(Cyp24a1)
MCAL110289544
MPAH110318266
RNO25279(Cyp24a1)
MCOC116091462
MUN110545262
CGE100751280
MAUA101827330
PLEU114704035
MORG121440893
MFOT126508008
AAMP119815492
NGI103735781
HGL101716773
CPOC100728021
CCAN109694681
DORD105991653
DSP122124591
NCAR124978047
OCU100347441
OPI101526808
TUP102471055
CFA485935(CYP24A1)
CLUD112674140
VVP112931920
VLG121477768
AML100479790
UMR103669826
UAH113258741
UAR123799375
ELK111141789
LLV125109083
MPUF101678691
ORO101384402
EJU114225492
ZCA113938600
MLX118024098
NSU110569858
FCA101096141
PYU121019910
PBG122467253
LRUF124504391
PTG102967619
PPAD109272242
AJU106970319
HHV120230459
BTA540080(CYP24A1)
BOM102272075
BIU109567562
BBUB102396198
CHX102178921
OAS100170323(CYP24)
ODA120869048
CCAD122448475
SSC397145(CYP24A1)
CFR102516204
CBAI105071834
CDK105098859
VPC102531787
BACU103015403
LVE103082411
OOR101271151
DLE111186695
PCAD102977275
PSIU116740225
ECB100053452(CYP24A1)
EPZ103554239
EAI106825434
MYB102249295
MYD102772049
MMYO118663454
MLF102432347
MNA107536509
PKL118708044
HAI109381840
DRO112304205
SHON118996516
AJM119040651
PDIC114506963
PHAS123815183
MMF118619039
RFQ117015976
PALE102878689
PGIG120603843
PVP105296033
RAY107498143
MJV108390269
TOD119254587
SARA101543781
LAV100663262
TMU101353460
DNM101442213
MDO100028177
GAS123233554
SHR100916072
PCW110206681
OAA100078505
GGA395827(CYP24A1)
PCOC116227703
MGP100541378
CJO107323241
NMEL110408247
APLA101797037
ACYG106041795
AFUL116495811
TGU100226727
LSR110472394
SCAN103818886
PMOA120507065
OTC121342986
PRUF121359459
GFR102044237
FAB101813067
PHI102111029
PMAJ107213207
CCAE111938004
CCW104687601
CBRC103616823
ETL114056255
ZAB102067744
ACHL103801335
FPG101919538
FCH102046853
CLV102098516
EGZ104132191
NNI104013059
PLET104624707
PCRI104024392
ACUN113487633
TALA104355745
PADL103919278
AFOR103894637
ACHC115339853
HALD104317033
CCRI104163872
CSTI104559611
EHS104503318
CMAC104483098
MUI104537354
FGA104069789
GSTE104258977
LDI104340282
MNB103775032
OHA104328853
NNT104412533
DPUB104302691
PGUU104464606
ACAR104526380
AVIT104274781
CVF104293941
AAM106491745
AROW112960579
NPD112949674
TGT104576182
DNE112982887
SCAM104150031
ASN102382614
AMJ102574966(CYP24A1)
CPOO109318764
GGN109292377
PSS102458142
CMY102932138
CPIC101938071
TST117886274
CABI116821477
MRV120379784
ACS100555767 100564552
PVT110071817
SUND121927742
PBI103065707
PMUR107287085
CTIG120308207
TSR106547483
PGUT117674844
VKO123036291
PMUA114599654
ZVI118089322
GJA107119104
STOW125432416
XLA108700906(cyp24a1.L) 108718538
XTR100496967 100497429(cyp24a1)
NPR108784249 108790437
RTEM120940805
BBUF121001334
BGAR122939129
DRE100004700(cyp24a1)
SRX107744352
SANH107666154 107696435
SGH107560513 107587600
CCAR109057669
CAUA113050135 113078343 113104946 113105072
PPRM120493676
MAMB125273354
IPU108271496
PHYP113532087
SMEO124388872
TFD113642663
AMEX103030576
EEE113571660
TRU101077963
TNGGSTEN00033861G001
LCO104935382
NCC104966082
CGOB115008593
ELY117257164
EFO125895063
PLEP121959102
SLUC116051531
ECRA117943678
ESP116687595
PFLV114554700
GAT120835793
PPUG119194098
MSAM119885885
CUD121507546
ALAT119020664
MZE101475325
ONL100690876
OAU116319460
OLA101165632
OML112145619
XMA102225814
XCO114135962
XHE116711044
PRET103464673
PFOR103134567
PLAI106960246
PMEI106929021
GAF122833415
CVG107097105
CTUL119788661
GMU124856320
NFU107385407
KMR108231477
ALIM106533681
NWH119411875
AOCE111579624
MCEP125006146
CSEM103386736
POV109635373
SSEN122767772
HHIP117761120
HSP118103196
LCF108873562
SDU111233601
SLAL111660685
XGL120783454
HCQ109507475
BPEC110171174
MALB109951265
BSPL114856188
SASA106565076(CP24A) 106583685
STRU115150083 115208378
OTW112255537 112267058
OMY100136661 110493887
OGO124002353 124018524
ONE115125232 115142853
SALP111961723 111970527
SNH120064455 120065468
CCLU121575591 121577964
ELS105016677
SFM108936451
PKI111835893
AANG118210803
LOC102698913
PSPA121297804 121319232
ARUT117418538 117433450
LCM102364004
CMK103191047
RTP109910917
HAME121870079
RSAN119392951
RMP119172898
GAE121366250 121373112
HRF124126383 124134044 124135907 124143865
HRJ124282302 124283687 124289154
XEN124454605
 » show all
Reference
1  [PMID:16516540]
  Authors
Masuda S, Strugnell SA, Knutson JC, St-Arnaud R, Jones G
  Title
Evidence for the activation of 1alpha-hydroxyvitamin D2 by 25-hydroxyvitamin D-24-hydroxylase: delineation of pathways involving 1alpha,24-dihydroxyvitamin D2 and 1alpha,25-dihydroxyvitamin D2.
  Journal
Biochim Biophys Acta 1761:221-34 (2006)
DOI:10.1016/j.bbalip.2006.01.004
Reference
2  [PMID:16617161]
  Authors
Hamamoto H, Kusudo T, Urushino N, Masuno H, Yamamoto K, Yamada S, Kamakura M, Ohta M, Inouye K, Sakaki T
  Title
Structure-function analysis of vitamin D 24-hydroxylase (CYP24A1) by site-directed mutagenesis: amino acid residues responsible for species-based difference of CYP24A1 between humans and rats.
  Journal
Mol Pharmacol 70:120-8 (2006)
DOI:10.1124/mol.106.023275
  Sequence
[rno:25279]
Reference
3  [PMID:15574355]
  Authors
Sakaki T, Kagawa N, Yamamoto K, Inouye K
  Title
Metabolism of vitamin D3 by cytochromes P450.
  Journal
Front Biosci 10:119-34 (2005)
DOI:10.2741/1514
  Sequence
[hsa:1591]
Reference
4  [PMID:17646648]
  Authors
Prosser DE, Kaufmann M, O'Leary B, Byford V, Jones G
  Title
Single A326G mutation converts human CYP24A1 from 25-OH-D3-24-hydroxylase into -23-hydroxylase, generating 1alpha,25-(OH)2D3-26,23-lactone.
  Journal
Proc Natl Acad Sci U S A 104:12673-8 (2007)
DOI:10.1073/pnas.0702093104
Reference
5  [PMID:15358094]
  Authors
Kusudo T, Sakaki T, Abe D, Fujishima T, Kittaka A, Takayama H, Hatakeyama S, Ohta M, Inouye K
  Title
Metabolism of A-ring diastereomers of 1alpha,25-dihydroxyvitamin D3 by CYP24A1.
  Journal
Biochem Biophys Res Commun 321:774-82 (2004)
DOI:10.1016/j.bbrc.2004.07.040
Reference
6  [PMID:15078099]
  Authors
Sawada N, Kusudo T, Sakaki T, Hatakeyama S, Hanada M, Abe D, Kamao M, Okano T, Ohta M, Inouye K
  Title
Novel metabolism of 1 alpha,25-dihydroxyvitamin D3 with C24-C25 bond cleavage catalyzed by human CYP24A1.
  Journal
Biochemistry 43:4530-7 (2004)
DOI:10.1021/bi030207f
Reference
7  [PMID:15544953]
  Authors
Prosser DE, Jones G
  Title
Enzymes involved in the activation and inactivation of vitamin D.
  Journal
Trends Biochem Sci 29:664-73 (2004)
DOI:10.1016/j.tibs.2004.10.005
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.16
IUBMB Enzyme Nomenclature: 1.14.15.16
ExPASy - ENZYME nomenclature database: 1.14.15.16
BRENDA, the Enzyme Database: 1.14.15.16
LinkDB

KEGG   REACTION: R09515
Entry
R09515                      Reaction                               
Name
calcitriol,adrenodoxin:oxygen oxidoreductase (24-hydroxylating)
Definition
Calcitriol + 2 Reduced adrenal ferredoxin + 2 H+ + Oxygen <=> Calcitetrol + 2 Oxidized adrenal ferredoxin + H2O
Equation
Reaction class
RC01223  C01673_C18231
Enzyme
Pathway
rn00100  Steroid biosynthesis
rn01100  Metabolic pathways
Orthology
K07436  vitamin D3 24-hydroxylase [EC:1.14.15.16]
Other DBs
RHEA: 24967
LinkDB

DBGET integrated database retrieval system