KEGG ORTHOLOGY: K07545

ORTHOLOGY: K07545

Entry

K07545                      KO

Symbol

bbsG

Name

(R)-benzylsuccinyl-CoA dehydrogenase [EC:1.3.8.3]

Pathway

map00623

Toluene degradation

map01100

Metabolic pathways

map01120

Microbial metabolism in diverse environments

map01220

Degradation of aromatic compounds

Module

M00418

Toluene degradation, anaerobic, toluene => benzoyl-CoA

Reaction

R05584

(R)-benzylsuccinyl-CoA:electron transfer flavoprotein oxidoreductase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K07545  bbsG; (R)-benzylsuccinyl-CoA dehydrogenase
Enzymes [BR:ko01000]
1. Oxidoreductases
  1.3  Acting on the CH-CH group of donors
   1.3.8  With a flavin as acceptor
    1.3.8.3  (R)-benzylsuccinyl-CoA dehydrogenase
     K07545  bbsG; (R)-benzylsuccinyl-CoA dehydrogenase

Genes

BFZ:	BAU07_03785

BGM:	CAL15_20545

ADY:	HLG70_07275

BOZ:	DBV39_02120

VEI:

Veis_2517

EBA:	c2A310(bbsG)

APET:

ToN1_01540(bbsG)

AZI:	AzCIB_4519

AZD:	CDA09_07455 CDA09_21960

TCL:

Tchl_1391

TAK:	Tharo_3084

GME:	Gmet_1523(bbsG)

GEO:	Geob_2422(bbsG)

DTO:	TOL2_C01530(bbsG)

DMM:	dnm_089810(bhsG)

DGI:	Desgi_0647

CHRE:

IE160_04110

AG:	AAF89842(bbsG)

» show all

Reference

PMID:10629170

Authors

Leuthner B, Heider J

Title

Anaerobic toluene catabolism of Thauera aromatica: the bbs operon codes for enzymes of beta oxidation of the intermediate benzylsuccinate.

Journal

J Bacteriol 182:272-7 (2000)
DOI:10.1128/JB.182.2.272-277.2000

Sequence

[ag:AAF89842]

Reference

PMID:12420174

Authors

Leutwein C, Heider J

Title

(R)-Benzylsuccinyl-CoA dehydrogenase of Thauera aromatica, an enzyme of the anaerobic toluene catabolic pathway.

Journal

Arch Microbiol 178:517-24 (2002)
DOI:10.1007/s00203-002-0484-5

Sequence

[ag:AAF89842]

LinkDB

ENZYME: 1.3.8.3

Entry

EC 1.3.8.3                  Enzyme

Name

(R)-benzylsuccinyl-CoA dehydrogenase;
BbsG;
(R)-benzylsuccinyl-CoA:(acceptor) oxidoreductase

Class

Oxidoreductases;
Acting on the CH-CH group of donors;
With a flavin as acceptor

Sysname

(R)-benzylsuccinyl-CoA:electron transfer flavoprotein oxidoreductase

Reaction(IUBMB)

(R)-2-benzylsuccinyl-CoA + electron-transfer flavoprotein = (E)-2-benzylidenesuccinyl-CoA + reduced electron-transfer flavoprotein

Reaction(KEGG)

(other) R05584

Substrate

(R)-2-benzylsuccinyl-CoA [CPD:C09817];
electron-transfer flavoprotein [CPD:C04253]

Product

(E)-2-benzylidenesuccinyl-CoA [CPD:C09818];
reduced electron-transfer flavoprotein [CPD:C04570]

Comment

Contains a tightly-bound FAD cofactor. Unlike other acyl-CoA dehydrogenases, this enzyme exhibits high substrate- and enantiomer specificity; it is highly specific for (R)-benzylsuccinyl-CoA and is inhibited by (S)-benzylsuccinyl-CoA. Forms the third step in the anaerobic toluene metabolic pathway in Thauera aromatica. Ferricenium ion is an effective artificial electron acceptor.

History

EC 1.3.8.3 created 2003 as EC 1.3.99.21, transferred 2012 to EC 1.3.8.3

Pathway

ec00623

Toluene degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K07545

(R)-benzylsuccinyl-CoA dehydrogenase

Genes

BFZ:	BAU07_03785

BGM:	CAL15_20545

ADY:	HLG70_07275

BOZ:	DBV39_02120

VEI:

Veis_2517

EBA:	c2A310(bbsG)

APET:

ToN1_01540(bbsG)

AZI:	AzCIB_4519

AZD:	CDA09_07455 CDA09_21960

TCL:

Tchl_1391

TAK:	Tharo_3084

GME:	Gmet_1523(bbsG)

GEO:	Geob_2422(bbsG)

DTO:	TOL2_C01530(bbsG)

DMM:	dnm_089810(bhsG)

DGI:	Desgi_0647

CHRE:

IE160_04110

» show all

Reference

1 [PMID:10589736]

Authors

Leutwein C, Heider J.

Title

Anaerobic toluene-catabolic pathway in denitrifying Thauera aromatica: activation and beta-oxidation of the first intermediate, (R)-(+)-benzylsuccinate.

Journal

Microbiology 145 ( Pt 11):3265-71 (1999)
DOI:10.1099/00221287-145-11-3265

Reference

2 [PMID:12420174]

Authors

Leutwein C, Heider J

Title

(R)-Benzylsuccinyl-CoA dehydrogenase of Thauera aromatica, an enzyme of the anaerobic toluene catabolic pathway.

Journal

Arch Microbiol 178:517-24 (2002)
DOI:10.1007/s00203-002-0484-5

Sequence

[ag:AAF89842]

Other DBs

ExplorEnz - The Enzyme Database:

1.3.8.3

IUBMB Enzyme Nomenclature:

1.3.8.3

ExPASy - ENZYME nomenclature database:

1.3.8.3

UM-BBD (Biocatalysis/Biodegradation Database):

1.3.8.3

BRENDA, the Enzyme Database:

1.3.8.3

LinkDB

REACTION: R05584

Entry

R05584                      Reaction

Name

(R)-benzylsuccinyl-CoA:electron transfer flavoprotein oxidoreductase

Definition

Benzylsuccinyl-CoA + 2 Electron-transferring flavoprotein <=> E-Phenylitaconyl-CoA + 2 Reduced electron-transferring flavoprotein

Equation

C09817 + 2 C04253 <=> C09818 + 2 C04570

Comment

BbsG

Reaction class

RC02037

C09817_C09818

Enzyme

1.3.8.3

Pathway

rn00623

Toluene degradation

rn01100

Metabolic pathways

rn01120

Microbial metabolism in diverse environments

rn01220

Degradation of aromatic compounds

Module

M00418

Toluene degradation, anaerobic, toluene => benzoyl-CoA

Brite

Enzymatic reactions [BR:br08201]
1. Oxidoreductase reactions
  1.3  Acting on the CH-CH group of donors
   1.3.8  With a flavin as acceptor
    1.3.8.3
     R05584  Benzylsuccinyl-CoA + 2 Electron-transferring flavoprotein <=> E-Phenylitaconyl-CoA + 2 Reduced electron-transferring flavoprotein

Orthology

K07545

(R)-benzylsuccinyl-CoA dehydrogenase [EC:1.3.8.3]

LinkDB

DBGET integrated database retrieval system