KEGG   ORTHOLOGY: K08590
Entry
K08590            Tight     KO                                     
Symbol
nit
Name
2-oxoglutaramate amidase [EC:3.5.1.111]
Pathway
map00250  Alanine, aspartate and glutamate metabolism
map01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00250 Alanine, aspartate and glutamate metabolism
    K08590  nit; 2-oxoglutaramate amidase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.5  Acting on carbon-nitrogen bonds, other than peptide bonds
   3.5.1  In linear amides
    3.5.1.111  2-oxoglutaramate amidase
     K08590  nit; 2-oxoglutaramate amidase
Other DBs
RN: R00269
COG: COG0388
Genes
PNV: JMY29_20620
AG: CAD47935(nit)
Reference
  Authors
Cobzaru C, Ganas P, Mihasan M, Schleberger P, Brandsch R
  Title
Homologous gene clusters of nicotine catabolism, including a new omega-amidase for alpha-ketoglutaramate, in species of three genera of Gram-positive bacteria.
  Journal
Res Microbiol 162:285-91 (2011)
DOI:10.1016/j.resmic.2011.01.001
  Sequence
LinkDB

KEGG   ENZYME: 3.5.1.111
Entry
EC 3.5.1.111                Enzyme                                 
Name
2-oxoglutaramate amidase;
omega-amidase (ambiguous)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
5-amino-2,5-dioxopentanoate amidohydrolase
Reaction(IUBMB)
2-oxoglutaramate + H2O = 2-oxoglutarate + NH3 [RN:R00269]
Reaction(KEGG)
R00269
Substrate
2-oxoglutaramate [CPD:C00940];
H2O [CPD:C00001]
Product
2-oxoglutarate [CPD:C00026];
NH3 [CPD:C00014]
Comment
The enzyme, which is highly specific for its substrate, participates in the nicotine degradation pathway of several Gram-positive bacteria.
History
EC 3.5.1.111 created 2012
Pathway
ec00250  Alanine, aspartate and glutamate metabolism
ec01100  Metabolic pathways
Orthology
K08590  2-oxoglutaramate amidase
Genes
PNVJMY29_20620
Reference
1  [PMID:21288482]
  Authors
Cobzaru C, Ganas P, Mihasan M, Schleberger P, Brandsch R
  Title
Homologous gene clusters of nicotine catabolism, including a new omega-amidase for alpha-ketoglutaramate, in species of three genera of Gram-positive bacteria.
  Journal
Res Microbiol 162:285-91 (2011)
DOI:10.1016/j.resmic.2011.01.001
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.111
IUBMB Enzyme Nomenclature: 3.5.1.111
ExPASy - ENZYME nomenclature database: 3.5.1.111
BRENDA, the Enzyme Database: 3.5.1.111
LinkDB

KEGG   REACTION: R00269
Entry
R00269                      Reaction                               
Name
2-oxoglutaramate amidohydrolase
Definition
2-Oxoglutaramate + H2O <=> 2-Oxoglutarate + Ammonia
Equation
Reaction class
RC00010  C00026_C00940
Enzyme
3.5.1.3         3.5.1.111
Pathway
rn00250  Alanine, aspartate and glutamate metabolism
rn01100  Metabolic pathways
Orthology
K08590  2-oxoglutaramate amidase [EC:3.5.1.111]
K13566  omega-amidase [EC:3.5.1.3]
Other DBs
RHEA: 32966
LinkDB

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