CoA [CPD:C00010];
(2R)-2-hydroxy-2-methylbutanedioate [CPD:C02612]
Comment
One of the enzymes involved in a pyruvate-derived pathway for isoleucine biosynthesis that is found in some bacterial and archaeal species [1,2]. The enzyme can be inhibited by isoleucine, the end-product of the pathway, but not by leucine [2]. The enzyme is highly specific for pyruvate as substrate, as the 2-oxo acids 3-methyl-2-oxobutanoate, 2-oxobutanoate, 4-methyl-2-oxopentanoate, 2-oxohexanoate and 2-oxoglutarate cannot act as substrate [1,2].
History
EC 2.3.3.21 created 2007 as EC 2.3.1.182, transferred 2021 to EC 2.3.3.21
Enzymatic reactions [BR:br08201]
2. Transferase reactions
2.3 Acyltransferases
2.3.3 Acyl groups converted into alkyl groups on transfer
2.3.3.21
R07399 Acetyl-CoA + Pyruvate + H2O <=> (R)-2-Methylmalate + CoA
