KEGG   ORTHOLOGY: K09473
Entry
K09473                      KO                                     
Symbol
puuD
Name
gamma-glutamyl-gamma-aminobutyrate hydrolase [EC:3.5.1.94]
Pathway
map00330  Arginine and proline metabolism
map01100  Metabolic pathways
Module
M00136  GABA biosynthesis, prokaryotes, putrescine => GABA
Reaction
R07419  4-(gamma-L-glutamylamino)butanoate amidohydrolase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00330 Arginine and proline metabolism
    K09473  puuD; gamma-glutamyl-gamma-aminobutyrate hydrolase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01002 Peptidases and inhibitors
    K09473  puuD; gamma-glutamyl-gamma-aminobutyrate hydrolase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.5  Acting on carbon-nitrogen bonds, other than peptide bonds
   3.5.1  In linear amides
    3.5.1.94  gamma-glutamyl-gamma-aminobutyrate hydrolase
     K09473  puuD; gamma-glutamyl-gamma-aminobutyrate hydrolase
Peptidases and inhibitors [BR:ko01002]
 Cysteine peptidases
  Family C26: gamma-glutamyl hydrolase family
   K09473  puuD; gamma-glutamyl-gamma-aminobutyrate hydrolase
Other DBs
COG: COG2071
GO: 0033969
Genes
ECO: b1298(puuD)
ECJ: JW1291(puuD)
ECD: ECDH10B_1415(puuD)
EBW: BWG_1130(puuD)
ECOK: ECMDS42_1096(puuD)
ECOC: C3026_07620(puuD)
ECE: Z2490(ycjL)
ECS: ECs_1875(puuD)
ECF: ECH74115_1940(puuD)
ETW: ECSP_1823(puuD)
ELX: CDCO157_1795(puuD)
EOI: ECO111_1681(puuD)
EOJ: ECO26_1865(puuD)
EOH: ECO103_1462(puuD)
ECOO: ECRM13514_1724(puuD)
ECOH: ECRM13516_1689(puuD)
ESL: O3K_13840(puuD)
ESO: O3O_11790(puuD)
ESM: O3M_13815(puuD)
ECK: EC55989_1460(puuD)
EOK: G2583_1645(puuD)
ELR: ECO55CA74_08010(puuD)
ELH: ETEC_1402
ECW: EcE24377A_1508(puuD)
ECOA: APECO78_10365(puuD)
ECX: EcHS_A1413(puuD)
ECM: EcSMS35_1824(puuD)
ECY: ECSE_1350
ECR: ECIAI1_1323(puuD)
EUM: ECUMN_1604(puuD)
ECT: ECIAI39_1649(puuD)
EOC: CE10_1546(puuD)
EBR: ECB_01275(ycjL)
ECOB: C3029_10940(puuD)
EBL: ECD_01275(puuD)
EBE: B21_01286(puuD)
EBD: ECBD_2319
ELO: EC042_1426(puuD)
EKF: KO11_16320(puuB)
EDJ: ECDH1ME8569_1240(puuD)
ELW: ECW_m1394(puuB)
ELL: WFL_06795(puuB)
ELP: P12B_c1796(puuD)
ECOL: LY180_06610(puuD)
EAL: EAKF1_ch0120c(puuD)
ESZ: FEM44_21590(puuD)
ERUY: OSH18_01830(puuD)
SFL: SF1303(puuD)
SFX: S1385(ycjL)
SFV: SFV_1312(ycjL)
SFE: SFxv_1476(puuD)
SFN: SFy_1872
SFS: SFyv_1930
SFT: NCTC1_01378(puuD)
SSN: SSON_1842(ycjL)
SHQ: A0259_10615(puuD)
ENL: A3UG_10015(puuD)
ENO: ECENHK_09940(puuD)
ECHG: FY206_11405(puuD)
EBG: FAI37_19215(puuD)
ENZ: G0034_09780(puuD)
EQU: OM418_09770(puuD)
EPU: QVH39_10220(puuD)
CSK: ES15_1440 ES15_2236(puuD)
CTU: CTU_18850(puuD) CTU_27130(puuD)
KPN: KPN_01020
KPU: KP1_2005
KPP: A79E_3213
KPO: KPN2242_08160(puuD)
KPR: KPR_3502(puuD)
KPJ: N559_3267
KPI: D364_05305(puuD)
KPX: PMK1_03360(puuD)
KPNU: LI86_16975
KPNK: BN49_2108(puuD)
KVA: Kvar_3359
KPE: KPK_3540(puuD)
KOX: KOX_16930(puuD)
KOE: A225_2234
KOY: J415_20605(puuD)
EAE: EAE_15930(puuD)
EAR: CCG30721
KAR: LGL98_16125(puuD)
KGR: JJJ10_17595(puuD)
KPAS: LUW96_17550(puuD)
KLP: GUC22_16570(puuD)
ROR: RORB6_09560(puuD)
RTG: NCTC13098_04767(puuD)
CRO: ROD_03571(puuD)
CPOT: FOB25_06245(puuD)
CITZ: E4Z61_12075(puuD)
CARS: E1B03_06250(puuD)
CIX: M4I31_18390(puuD)
CIB: HF677_018365(puuD)
CITR: GUC46_18325(puuD)
BSEL: RHD99_09170(puuD)
YRE: HEC60_09605(puuD)
SGOE: A8O29_008300(puuD)
IZH: FEM41_12210(puuD)
PSGC: G163CM_17500(puuD)
TOE: QMG90_13645(puuD)
EBF: D782_2780
EBB: F652_550
PSTS: E05_49390
SMAR: SM39_1530(puuD)
SMAC: SMDB11_1340(puuD)
SMW: SMWW4_v1c20860(puuD)
SPE: Spro_2067
SRL: SOD_c18790(puuD)
SRY: M621_10365(puuD)
SPLY: Q5A_010395(puuD)
SMAF: D781_1891
SLQ: M495_10020(puuD)
SERF: L085_18335(puuD)
SERS: SERRSCBI_09775(puuD)
SQU: E4343_05880(puuD)
SFJ: SAMEA4384070_2124(puuD)
SOF: NCTC11214_04759(puuD)
SURI: J0X03_13570(puuD)
SRHZ: FO014_00160(puuD)
SENP: KHA73_10455(puuD)
SNEM: NLX84_10515(puuD)
SNEV: OI978_22830(puuD)
SGRI: SGBXF1_02083(puuD)
SSAR: SSARUM_001993(puuD)
RACE: JHW33_02135(puuD)
RAA: Q7S_07625(puuD)
RVC: J9880_15625(puuD)
RBON: QNM34_08140(puuD)
RIU: I2123_16130(puuD)
EAME: GXP68_07185(puuD)
RBAD: H2866_00980(puuD)
RCB: O1V66_02915(puuD)
CARU: P0E69_06625(puuD)
EBI: EbC_29020(puuD)
ERWI: GN242_07300(puuD)
ERP: LJN55_15060(puuD)
EPI: Q3V30_07535(puuD)
ETO: RIN69_14700(puuD)
EGE: EM595_2350(puuD)
PAM: PANA_2463(puuD)
PLF: PANA5342_1623(ycjL)
PAJ: PAJ_1760(puuD)
PVA: Pvag_1946(ycjL)
PSTW: DSJ_15840
PANS: FCN45_12315(puuD)
PEY: EE896_06980(puuD)
PER: LAC65_09785(puuD)
PJI: KTJ90_06750(puuD)
PANO: OJ965_09205(puuD)
KPIE: N5580_06300(puuD)
PALF: K6R05_06840(puuD)
PDIP: KFZ74_07640(puuD)
MINT: C7M51_01857(puuD)
MTHI: C7M52_00953(puuD)
MHAN: K6958_13035(puuD)
PMAK: PMPD1_2814
LCJ: NCTC11976_00395(pyrG_1)
OCE: GU3_14520(puuD)
OPE: PU634_02245(puuD)
DNO: DNO_0653
 » show all
Reference
  Authors
Kurihara S, Oda S, Kato K, Kim HG, Koyanagi T, Kumagai H, Suzuki H.
  Title
A novel putrescine utilization pathway involves gamma-glutamylated intermediates of Escherichia coli K-12.
  Journal
J Biol Chem 280:4602-8 (2005)
DOI:10.1074/jbc.M411114200
  Sequence
[eco:b1298]
LinkDB

KEGG   ENZYME: 3.5.1.94
Entry
EC 3.5.1.94                 Enzyme                                 
Name
gamma-glutamyl-gamma-aminobutyrate hydrolase;
gamma-glutamyl-GABA hydrolase;
PuuD;
YcjL;
4-(gamma-glutamylamino)butanoate amidohydrolase;
4-(L-gamma-glutamylamino)butanoate amidohydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
4-(gamma-L-glutamylamino)butanoate amidohydrolase
Reaction(IUBMB)
4-(gamma-L-glutamylamino)butanoate + H2O = 4-aminobutanoate + L-glutamate [RN:R07419]
Reaction(KEGG)
R07419
Substrate
4-(gamma-L-glutamylamino)butanoate [CPD:C15767];
H2O [CPD:C00001]
Product
4-aminobutanoate [CPD:C00334];
L-glutamate [CPD:C00025]
Comment
Forms part of a putrescine-utilizing pathway in Escherichia coli, in which it has been hypothesized that putrescine is first glutamylated to form gamma-glutamylputrescine, which is oxidized to 4-(gamma-glutamylamino)butanal and then to 4-(gamma-glutamylamino)butanoate. The enzyme can also catalyse the reactions of EC 3.5.1.35 (D-glutaminase) and EC 3.5.1.65 (theanine hydrolase).
History
EC 3.5.1.94 created 2006, modified 2011
Pathway
ec00330  Arginine and proline metabolism
ec01100  Metabolic pathways
Orthology
K09473  gamma-glutamyl-gamma-aminobutyrate hydrolase
Genes
ECOb1298(puuD)
ECJJW1291(puuD)
ECDECDH10B_1415(puuD)
EBWBWG_1130(puuD)
ECOKECMDS42_1096(puuD)
ECOCC3026_07620(puuD)
ECEZ2490(ycjL)
ECSECs_1875(puuD)
ECFECH74115_1940(puuD)
ETWECSP_1823(puuD)
ELXCDCO157_1795(puuD)
EOIECO111_1681(puuD)
EOJECO26_1865(puuD)
EOHECO103_1462(puuD)
ECOOECRM13514_1724(puuD)
ECOHECRM13516_1689(puuD)
ESLO3K_13840(puuD)
ESOO3O_11790(puuD)
ESMO3M_13815(puuD)
ECKEC55989_1460(puuD)
EOKG2583_1645(puuD)
ELRECO55CA74_08010(puuD)
ELHETEC_1402
ECWEcE24377A_1508(puuD)
EUNUMNK88_1634
ECOAAPECO78_10365(puuD)
ECXEcHS_A1413(puuD)
ECMEcSMS35_1824(puuD)
ECYECSE_1350
ECRECIAI1_1323(puuD)
EUMECUMN_1604(puuD)
ECTECIAI39_1649(puuD)
EOCCE10_1546(puuD)
EBRECB_01275(ycjL)
ECOBC3029_10940(puuD)
EBLECD_01275(puuD)
EBEB21_01286(puuD)
EBDECBD_2319
ELOEC042_1426(puuD)
ECLEcolC_2327
EKOEKO11_2552
EKFKO11_16320(puuB)
EDHEcDH1_2348
EDJECDH1ME8569_1240(puuD)
ELWECW_m1394(puuB)
ELLWFL_06795(puuB)
ELPP12B_c1796(puuD)
ECOLLY180_06610(puuD)
EALEAKF1_ch0120c(puuD)
ESZFEM44_21590(puuD)
ERUYOSH18_01830(puuD)
SFLSF1303(puuD)
SFXS1385(ycjL)
SFVSFV_1312(ycjL)
SFESFxv_1476(puuD)
SFNSFy_1872
SFSSFyv_1930
SFTNCTC1_01378(puuD)
SSNSSON_1842(ycjL)
SDZAsd1617_02621
SHQA0259_10615(puuD)
ENCECL_02226 ECL_02227
ENLA3UG_10015(puuD)
ECLGEC036_19400
ECLNECNIH4_12705
EAUDI57_08925
EKBBFV64_10365
ENOECENHK_09940(puuD)
ECANCWI88_14360
ERNBFV67_09510
ECHGFY206_11405(puuD)
EBGFAI37_19215(puuD)
ENDA4308_14655
ENZG0034_09780(puuD)
ENBELK40_10330
EQUOM418_09770(puuD)
EPUQVH39_10220(puuD)
ESAESA_01202 ESA_02079
CSKES15_1440 ES15_2236(puuD)
CSZCSSP291_05720 CSSP291_09885(puuD)
CSJCSK29544_02519 CSK29544_03372
CCONAFK62_12455
CDMAFK67_08760 AFK67_13070
CSIP262_02096 P262_03230
CMJAFK66_007010 AFK66_010920
CUIAFK65_08620 AFK65_12765
CMWAFK63_05570 AFK63_09870
CTUCTU_18850(puuD) CTU_27130(puuD)
KPNKPN_01020
KPUKP1_2005
KPMKPHS_18950
KPPA79E_3213
KPHKPNIH24_19035
KPZKPNIH27_08950
KPVKPNIH29_09480
KPWKPNIH30_09995
KPYKPNIH31_09370
KPGKPNIH32_09835
KPCKPNIH10_09490
KPQKPR0928_09660
KPTVK055_1457
KPOKPN2242_08160(puuD)
KPRKPR_3502(puuD)
KPJN559_3267
KPID364_05305(puuD)
KPAKPNJ1_03508
KPSKPNJ2_03497
KPXPMK1_03360(puuD)
KPBFH42_00955
KPNEKU54_016665
KPNULI86_16975
KPNKBN49_2108(puuD)
KVAKvar_3359
KPEKPK_3540(puuD)
KPKA593_19600
KVDKR75_17270
KVQSP68_23650
KOXKOX_16930(puuD)
KOEA225_2234
KOYJ415_20605(puuD)
KOMHR38_15450
KMIVW41_14390
KOKKONIH1_10930
KOCAB185_25205
KQUAVR78_13930
EAEEAE_15930(puuD)
EARCCG30721
KQVB8P98_18170
KLLBJF97_11205
KLWDA718_17645
KARLGL98_16125(puuD)
KGRJJJ10_17595(puuD)
KPASLUW96_17550(puuD)
KLMBWI76_10705
KLPGUC22_16570(puuD)
RORRORB6_09560(puuD)
RONTE10_11020
RPLNB1209_17455
RAODSD31_16195
RTGNCTC13098_04767(puuD)
CROROD_03571(puuD)
CFDCFNIH1_11620
CBRAA6J81_11670
CYOCD187_18790
CPOTFOB25_06245(puuD)
CFQC2U38_05160
CIEAN232_24815
CITZE4Z61_12075(puuD)
CARSE1B03_06250(puuD)
CIXM4I31_18390(puuD)
CIBHF677_018365(puuD)
CITRGUC46_18325(puuD)
CNTJT31_20730
CEMLH23_20345
CENLH86_18980
PGELG71_13295
ESCEntcl_1709
KLEAO703_13445
LAZA8A57_07990
BSELRHD99_09170(puuD)
YREHEC60_09605(puuD)
SGOEA8O29_008300(puuD)
IZHFEM41_12210(puuD)
PSGCG163CM_17500(puuD)
TOEQMG90_13645(puuD)
EBFD782_2780
EBUCUC76_06185
EBBF652_550
PSTSE05_49390
SMARSM39_1530(puuD)
SMACSMDB11_1340(puuD)
SMWSMWW4_v1c20860(puuD)
SPESpro_2067
SRRSerAS9_2004
SRLSOD_c18790(puuD)
SRYM621_10365(puuD)
SPLYQ5A_010395(puuD)
SRSSerAS12_2004
SRASerAS13_2005
SMAFD781_1891
SLQM495_10020(puuD)
SERFL085_18335(puuD)
SERSSERRSCBI_09775(puuD)
SFWWN53_19615
SFGAV650_14990
SRZAXX16_3238
SERMCLM71_09390
SQUE4343_05880(puuD)
SFJSAMEA4384070_2124(puuD)
SOFNCTC11214_04759(puuD)
SSURATE40_006925
SURIJ0X03_13570(puuD)
SRHZFO014_00160(puuD)
SENPKHA73_10455(puuD)
SNEMNLX84_10515(puuD)
SNEVOI978_22830(puuD)
SGRISGBXF1_02083(puuD)
SSARSSARUM_001993(puuD)
SFOZ042_23795
RAHRahaq_1577
RACEJHW33_02135(puuD)
RAQRahaq2_1641
RAAQ7S_07625(puuD)
ROXBV494_03020
RVCJ9880_15625(puuD)
RBONQNM34_08140(puuD)
RIUI2123_16130(puuD)
EAMEGXP68_07185(puuD)
RBADH2866_00980(puuD)
RCBO1V66_02915(puuD)
CARUP0E69_06625(puuD)
NIGC1N62_05485
EBIEbC_29020(puuD)
EPECI789_04200
ERWIGN242_07300(puuD)
ERPLJN55_15060(puuD)
EPIQ3V30_07535(puuD)
ETORIN69_14700(puuD)
EGEEM595_2350(puuD)
PAMPANA_2463(puuD)
PLFPANA5342_1623(ycjL)
PAJPAJ_1760(puuD)
PAQPAGR_g1566
PVAPvag_1946(ycjL)
PAGGAL522_16625
PAOPat9b_2555
KLNLH22_09815
PANTPSNIH1_15800
PANPPSNIH2_05245
PAGCBEE12_00320
PSTWDSJ_15840
PALHB1H58_14670
PANSFCN45_12315(puuD)
PEYEE896_06980(puuD)
PDISD8B20_10935
PERLAC65_09785(puuD)
PJIKTJ90_06750(puuD)
PANOOJ965_09205(puuD)
KPIEN5580_06300(puuD)
PALFK6R05_06840(puuD)
PPHOCTZ24_15820
PDIPKFZ74_07640(puuD)
PGZC2E15_13345
PCDC2E16_13555
MINTC7M51_01857(puuD)
MTHIC7M52_00953(puuD)
MHANK6958_13035(puuD)
PMAKPMPD1_2814
HAVAT03_07545
HPARAL518_18045
OPODSM2777_12830
PSOPSYCG_04620
PSPGAK823_04435
PSYGAK825_04405
LCJNCTC11976_00395(pyrG_1)
ZPLZBT109_0404
HAAA5892_03645
OCEGU3_14520(puuD)
OPEPU634_02245(puuD)
ZDFAN401_15155
DNODNO_0653
SKGKJK29_10365
 » show all
Reference
1  [PMID:15590624]
  Authors
Kurihara S, Oda S, Kato K, Kim HG, Koyanagi T, Kumagai H, Suzuki H.
  Title
A novel putrescine utilization pathway involves gamma-glutamylated intermediates of Escherichia coli K-12.
  Journal
J Biol Chem 280:4602-8 (2005)
DOI:10.1074/jbc.M411114200
  Sequence
[eco:b1298]
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.94
IUBMB Enzyme Nomenclature: 3.5.1.94
ExPASy - ENZYME nomenclature database: 3.5.1.94
BRENDA, the Enzyme Database: 3.5.1.94
LinkDB

KEGG   REACTION: R07419
Entry
R07419                      Reaction                               
Name
4-(gamma-L-glutamylamino)butanoate amidohydrolase
Definition
4-(L-gamma-Glutamylamino)butanoate + H2O <=> 4-Aminobutanoate + L-Glutamate
Equation
Reaction class
RC00090  C00025_C15767
RC00096  C00334_C15767
Enzyme
Pathway
rn00330  Arginine and proline metabolism
rn01100  Metabolic pathways
Module
M00136  GABA biosynthesis, prokaryotes, putrescine => GABA
Brite
Enzymatic reactions [BR:br08201]
 3. Hydrolase reactions
  3.5  Acting on carbon-nitrogen bonds, other than peptide bonds
   3.5.1  In linear amides
    3.5.1.94
     R07419  4-(L-gamma-Glutamylamino)butanoate + H2O <=> 4-Aminobutanoate + L-Glutamate
Orthology
K09473  gamma-glutamyl-gamma-aminobutyrate hydrolase [EC:3.5.1.94]
Other DBs
RHEA: 19740
LinkDB

DBGET integrated database retrieval system