KEGG   ORTHOLOGY: K09588
Entry
K09588                      KO                                     
Symbol
CYP90A1, CPD
Name
3beta,22alpha-dihydroxysteroid 3-dehydrogenase [EC:1.14.19.79]
Pathway
map00905  Brassinosteroid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00371  Castasterone biosynthesis, campesterol => castasterone
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00905 Brassinosteroid biosynthesis
    K09588  CYP90A1, CPD; 3beta,22alpha-dihydroxysteroid 3-dehydrogenase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K09588  CYP90A1, CPD; 3beta,22alpha-dihydroxysteroid 3-dehydrogenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.79  3beta,22alpha-dihydroxysteroid 3-dehydrogenase
     K09588  CYP90A1, CPD; 3beta,22alpha-dihydroxysteroid 3-dehydrogenase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP90 family
   K09588  CYP90A1, CPD; 3beta,22alpha-dihydroxysteroid 3-dehydrogenase
Other DBs
RN: R07446 R12965
Genes
ATH: AT5G05690(CPD)
ALY: 9309287
CRB: 17884348
CSAT: 104708544 104734575 104768762
EUS: EUTSA_v10013418mg
BRP: 103846910 103850594 103855637
BNA: 106380838 106386327 106386485 106419352 106422628 106439685 106440690 106442975
BOE: 106318190 106322573
RSZ: 108816976 108841893 108845671
THJ: 104816550 104823148
CPAP: 110819283
CIT: 102608450
PVY: 116111729
TCC: 18585886
DZI: 111310123
LJA: Lj2g3v2746640.1(Lj2g3v2746640.1) Lj6g3v0524000.1(Lj6g3v0524000.1)
AIP: 107623684
FVE: 101301332
RCN: 112182961
PPER: 18785088
PMUM: 103332773
PAVI: 110749731
PDUL: 117617356
ZJU: 107413844
MNT: 21409069
CSV: 101216594
CMO: 103501437
BHJ: 120081203
MCHA: 111023728
CMOS: 111458347
RCU: 8261035
JCU: 105646342
QSU: 112011086
VVI: 100258692
VRI: 117929163
SLY: 101255105(CYP90A5)
SPEN: 107023593
SOT: 102604556
SSTN: 125874177
CANN: 107873297
NTA: 107785062(CYP90) 107791859
NSY: 104236880
NTO: 104097076
NAU: 109234452
SIND: 105160880
OEU: 111380564
EGT: 105977877
DCR: 108210725
CSIN: 114303283
BVG: 104903459
MING: 122093477
TSS: 122672797
DOSA: Os11t0143200-01(Os11g0143200) Os12t0139300-01(Os12g0139300)
BDI: 100831598
ATS: 109745725
TUA: 125508677
SBI: 8085636
PDA: 103716536
EGU: 105046233
AOF: 109829756
ATR: 18437942
 » show all
Reference
PMID:8612270
  Authors
Szekeres M, Nemeth K, Koncz-Kalman Z, Mathur J, Kauschmann A, Altmann T, Redei GP, Nagy F, Schell J, Koncz C
  Title
Brassinosteroids rescue the deficiency of CYP90, a cytochrome P450, controlling cell elongation and de-etiolation in Arabidopsis.
  Journal
Cell 85:171-82 (1996)
DOI:10.1016/s0092-8674(00)81094-6
  Sequence
[ath:AT5G05690]
Reference
  Authors
Bancos S, Nomura T, Sato T, Molnar G, Bishop GJ, Koncz C, Yokota T, Nagy F, Szekeres M
  Title
Regulation of transcript levels of the Arabidopsis cytochrome p450 genes involved in brassinosteroid biosynthesis.
  Journal
Plant Physiol 130:504-13 (2002)
DOI:10.1104/pp.005439
Reference
  Authors
Ohnishi T, Godza B, Watanabe B, Fujioka S, Hategan L, Ide K, Shibata K, Yokota T, Szekeres M, Mizutani M.
  Title
CYP90A1/CPD, a brassinosteroid biosynthetic cytochrome P450 of Arabidopsis, catalyzes C-3 oxidation.
  Journal
J Biol Chem 287:31551-60 (2012)
DOI:10.1074/jbc.M112.392720
  Sequence
[ath:AT5G05690]
LinkDB

KEGG   ENZYME: 1.14.19.79
Entry
EC 1.14.19.79               Enzyme                                 
Name
3beta,22alpha-dihydroxysteroid 3-dehydrogenase;
CYP90A1 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
3beta,22alpha-dihydroxysteroid,[reduced NADPH-hemoprotein reductase]:oxygen 3-oxidoreductase
Reaction(IUBMB)
(1) (22S)-22-hydroxycampesterol + [reduced NADPH-hemoprotein reductase] + O2 = (22S)-22-hydroxycampest-4-en-3-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O [RN:R07446];
(2) 6-deoxoteasterone + [reduced NADPH-hemoprotein reductase] + O2 = 3-dehydro-6-deoxoteasterone + [oxidized NADPH-hemoprotein reductase] + 2 H2O [RN:R12965]
Reaction(KEGG)
R07446 R12965
Substrate
(22S)-22-hydroxycampesterol [CPD:C15795];
[reduced NADPH-hemoprotein reductase];
O2 [CPD:C00007];
6-deoxoteasterone [CPD:C15799]
Product
(22S)-22-hydroxycampest-4-en-3-one [CPD:C15796];
[oxidized NADPH-hemoprotein reductase];
H2O [CPD:C00001];
3-dehydro-6-deoxoteasterone [CPD:C15800]
Comment
This cytochrome P-450 (heme-thiolate) enzyme, characterized from the plant Arabidopsis thaliana, catalyses C-3 dehydrogenation of all 3beta-hydroxy brassinosteroid synthesis intermediates with 22-hydroxylated or 22,23-dihydroxylated side chains.
History
EC 1.14.19.79 created 2022
Pathway
ec00905  Brassinosteroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K09588  3beta,22alpha-dihydroxysteroid 3-dehydrogenase
Genes
ATHAT5G05690(CPD)
ALY9309287
CRB17884348
CSAT104708544 104734575 104768762
EUSEUTSA_v10013418mg
BRP103846910 103850594 103855637
BNA106380838 106386327 106386485 106419352 106422628 106439685 106440690 106442975
BOE106318190 106322573
RSZ108816976 108841893 108845671
THJ104816550 104823148
CPAP110819283
CIT102608450
CICCICLE_v10031576mg
PVY116111729
MINC123192846 123227420
TCC18585886
GRA105773896 105776399
GHI107931824 121203585 121222638 121230741
GAB108464090 108484595
DZI111310123
EGR104421095 104454392
GMX100775886 100781291 100789842 100795125(CYP90A15)
GSJ114382781 114382937 114395558 114399102
PVUPHAVU_001G242200g PHAVU_008G224600g
VRA106757945 106763863
VAR108329739 108336275
VUN114162864 114193700
CCAJ109790810 109805365
APRC113847685 113850044
MTRMTR_3g070380 MTR_5g082520
CAM101495460 101502086
LJALj2g3v2746640.1(Lj2g3v2746640.1) Lj6g3v0524000.1(Lj6g3v0524000.1)
ADU107457722 107469471 107469499
AIP107623684
AHF112702383 112715406 112715407 112765910 112784909
LANG109338900 109345069
FVE101301332
RCN112182961
PPER18785088
PMUM103332773
PAVI110749731
PDUL117617356
MDM103432823 103437230
PXB103936683 103958600
ZJU107413844
MNT21409069
CSV101216594
CMO103501437
BHJ120081203
MCHA111023728
CMAX111484767 111490939
CMOS111458347
CPEP111782190 111796648
RCU8261035
JCU105646342
HBR110646453 110651697
MESC110619393 110624089 122724866
POP7474440 7494485
PEU105120258 105133596 105139650
PALZ118043321 118055866
JRE108983029 108991904
QSU112011086
TWL120014341 120015942
VVI100258692
VRI117929163
SLY101255105(CYP90A5)
SPEN107023593
SOT102604556
SSTN125874177
CANN107873297
NTA107785062(CYP90) 107791859
NSY104236880
NTO104097076
NAU109234452
INI109148237 109186937
ITR116000029 116013913
SIND105160880
OEU111380564
EGT105977877
SSPL121773410 121776177 121796729
HAN110872702 110912986
ECAD122585038 122586969
LSV111896789 111919719
CCAV112509778 112525947
DCR108210725
CSIN114303283
BVG104903459
SOE110785503 110802180
CQI110699265 110714661 110725236 110733431
NNU104595015 104601254
MING122093477
TSS122672797
PSOM113313306 113314280 113323522 113338994
NCOL116253817 116256159
OSA4349746 4351461
DOSAOs11t0143200-01(Os11g0143200) Os12t0139300-01(Os12g0139300)
OBR102701391 102719724
BDI100831598
ATS109745725
TDC119302433 119308324
TAES123103027 123111228 123120303
TUA125508677
SBI8085636
ZMA100272666 103647105
SITA101755217 101768081
SVS117865869 117866170
PVIR120643525 120663533 120696674
PHAI112876861 112884910 112901899
PDA103716536
EGU105046233
MUS103977709 103994415
DCT110101203 110108808
PEQ110022516 110034891 110035737
AOF109829756
ATR18437942
 » show all
Reference
1  [PMID:22822057]
  Authors
Ohnishi T, Godza B, Watanabe B, Fujioka S, Hategan L, Ide K, Shibata K, Yokota T, Szekeres M, Mizutani M.
  Title
CYP90A1/CPD, a brassinosteroid biosynthetic cytochrome P450 of Arabidopsis, catalyzes C-3 oxidation.
  Journal
J Biol Chem 287:31551-60 (2012)
DOI:10.1074/jbc.M112.392720
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.79
IUBMB Enzyme Nomenclature: 1.14.19.79
ExPASy - ENZYME nomenclature database: 1.14.19.79
BRENDA, the Enzyme Database: 1.14.19.79
LinkDB

KEGG   REACTION: R12965
Entry
R12965                      Reaction                               
Name
6-deoxoteasterone,[reduced NADPH-hemoprotein reductase]:oxygen 3-oxidoreductase
Definition
6-Deoxoteasterone + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> 3-Dehydro-6-deoxoteasterone + [Oxidized NADPH---hemoprotein reductase] + 2 H2O
Equation
Reaction class
RC00144  C15799_C15800
Enzyme
Pathway
rn00905  Brassinosteroid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K09588  3beta,22alpha-dihydroxysteroid 3-dehydrogenase [EC:1.14.19.79]
LinkDB

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