KEGG   ORTHOLOGY: K10619
Entry
K10619                      KO                                     
Symbol
cmtAb
Name
p-cumate 2,3-dioxygenase subunit alpha [EC:1.14.12.25]
Pathway
map00622  Xylene degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01220  Degradation of aromatic compounds
Module
M00539  Cumate degradation, p-cumate => 2-oxopent-4-enoate + 2-methylpropanoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00622 Xylene degradation
    K10619  cmtAb; p-cumate 2,3-dioxygenase subunit alpha
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.25  p-cumate 2,3-dioxygenase
     K10619  cmtAb; p-cumate 2,3-dioxygenase subunit alpha
Dioxygenases [br01602.html]
 Dioxygenases in aromatic ring cleavage
  K10619
Other DBs
RN: R05247
COG: COG4638
GO: 0018570
Genes
RBD: ALSL_1087
PAE: PA2083
PAEV: N297_2148
PAEI: N296_2148
PAU: PA14_37570
PAG: PLES_32381
PAF: PAM18_2960
PNC: NCGM2_3063
PAEB: NCGM1900_4398
PDK: PADK2_15235
PAEP: PA1S_15265
PAEM: U769_14865
PAEL: T223_16545
PAEG: AI22_18505
PAEC: M802_2145
PAEO: M801_2147
PPF: Pput_2898
PPX: T1E_4248(cbdC)
PMON: X969_10845
PMOT: X970_10500
PSES: PSCI_1388
BXE: Bxe_A3556
BXB: DR64_1255
BARH: WN72_35375
SMAZ: LH19_27420
SWI: Swit_1758
SPHD: HY78_16795
SECH: B18_22315
MMAN: MMAN_42610
MSAK: MSAS_43630
MCOO: MCOO_07720
MHAS: MHAS_04026(cbdA_2)
MANY: MANY_01940
ROP: ROP_46340(cmtA1)
RRT: 4535765_00187(cmtA1_1) 4535765_00375(cmtA1_2)
SALS: SLNWT_6907
SHUN: DWB77_04977(antA)
PSIM: KR76_24915
AMQ: AMETH_1199(benA)
PDX: Psed_4287
 » show all
Reference
PMID:8631713
  Authors
Eaton RW.
  Title
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon.
  Journal
J Bacteriol 178:1351-62 (1996)
DOI:10.1128/JB.178.5.1351-1362.1996
  Sequence
[ppf:Pput_2898]
LinkDB

KEGG   ORTHOLOGY: K16303
Entry
K16303                      KO                                     
Symbol
cmtAc
Name
p-cumate 2,3-dioxygenase subunit beta [EC:1.14.12.25]
Pathway
map00622  Xylene degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01220  Degradation of aromatic compounds
Module
M00539  Cumate degradation, p-cumate => 2-oxopent-4-enoate + 2-methylpropanoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00622 Xylene degradation
    K16303  cmtAc; p-cumate 2,3-dioxygenase subunit beta
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.25  p-cumate 2,3-dioxygenase
     K16303  cmtAc; p-cumate 2,3-dioxygenase subunit beta
Dioxygenases [br01602.html]
 Dioxygenases in aromatic ring cleavage
  K16303
Other DBs
RN: R05247
GO: 0018570
Genes
RBD: ALSL_1088
PAE: PA2085
PAEV: N297_2150
PAEI: N296_2150
PAU: PA14_37550
PAG: PLES_32361
PAF: PAM18_2958
PNC: NCGM2_3065
PAEB: NCGM1900_4396
PDK: PADK2_15225
PAEP: PA1S_15255
PAEM: U769_14855
PAEL: T223_16535
PAEG: AI22_18515
PAEC: M802_2147
PAEO: M801_2149
PPF: Pput_2897
PPX: T1E_4249
PMON: X969_10850
PMOT: X970_10505
PSES: PSCI_1386
BXE: Bxe_A3555
BXB: DR64_1254
BARH: WN72_35380
SMAZ: LH19_27425
SWI: Swit_1757
SPHD: HY78_16800
SECH: B18_22325
MMAN: MMAN_42620
MSAK: MSAS_43620
MCOO: MCOO_07710
MHAS: MHAS_04025(bnzB)
MANY: MANY_01930
ROP: ROP_46330(cmtA2)
RRT: 4535765_00188(cmtA2_1) 4535765_00374(cmtA2_2)
SALS: SLNWT_6906
SHUN: DWB77_04978(hcaF)
PSIM: KR76_24910
PDX: Psed_4289
PRY: Prubr_43520(hcaF)
 » show all
Reference
PMID:8631713
  Authors
Eaton RW.
  Title
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon.
  Journal
J Bacteriol 178:1351-62 (1996)
DOI:10.1128/JB.178.5.1351-1362.1996
  Sequence
[ppf:Pput_2897]
LinkDB

KEGG   ORTHOLOGY: K16304
Entry
K16304                      KO                                     
Symbol
cmtAd
Name
p-cumate 2,3-dioxygenase ferredoxin component
Pathway
map00622  Xylene degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01220  Degradation of aromatic compounds
Module
M00539  Cumate degradation, p-cumate => 2-oxopent-4-enoate + 2-methylpropanoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00622 Xylene degradation
    K16304  cmtAd; p-cumate 2,3-dioxygenase ferredoxin component
Dioxygenases [br01602.html]
 Dioxygenases in aromatic ring cleavage
  K16304
Other DBs
RN: R05247
Genes
PPF: Pput_2894
PPX: T1E_4252
PMON: X969_10865
PMOT: X970_10520
SPOI: IMCC21906_02717
BXE: Bxe_A3552
BXB: DR64_1251(bphA3)
FLE: KI610_13205
BRK: CWS35_25080
BARH: WN72_35390
SWI: Swit_0969
SPHD: HY78_21670
MSAK: MSAS_43600(hcaC)
ROP: ROP_46320(cmtA3)
SALS: SLNWT_6905
 » show all
Reference
PMID:8631713
  Authors
Eaton RW.
  Title
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon.
  Journal
J Bacteriol 178:1351-62 (1996)
DOI:10.1128/JB.178.5.1351-1362.1996
  Sequence
[ppf:Pput_2894]
LinkDB

KEGG   ORTHOLOGY: K18227
Entry
K18227                      KO                                     
Symbol
cmtAa
Name
p-cumate 2,3-dioxygenase ferredoxin reductase component [EC:1.18.1.3]
Pathway
map00622  Xylene degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01220  Degradation of aromatic compounds
Module
M00539  Cumate degradation, p-cumate => 2-oxopent-4-enoate + 2-methylpropanoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00622 Xylene degradation
    K18227  cmtAa; p-cumate 2,3-dioxygenase ferredoxin reductase component
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.3  ferredoxin---NAD+ reductase
     K18227  cmtAa; p-cumate 2,3-dioxygenase ferredoxin reductase component
Dioxygenases [br01602.html]
 Dioxygenases in aromatic ring cleavage
  K18227
Other DBs
RN: R05247
Genes
PPF: Pput_2899
PPX: T1E_4247(camA)
PMON: X969_10840
PMOT: X970_10495
BXE: Bxe_A3557
BXB: DR64_1256(bphA4)
FLE: KI610_13210
MON: G8E03_15155
Reference
PMID:8631713
  Authors
Eaton RW.
  Title
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon.
  Journal
J Bacteriol 178:1351-62 (1996)
DOI:10.1128/JB.178.5.1351-1362.1996
  Sequence
[ppf:Pput_2899]
LinkDB

KEGG   ENZYME: 1.14.12.25
Entry
EC 1.14.12.25               Enzyme                                 
Name
p-cumate 2,3-dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
Sysname
4-isopropylbenzoate:oxygen 2,3-oxidoreductase
Reaction(IUBMB)
p-cumate + NADH + H+ + O2 = (2R,3S)-2,3-dihydroxy-2,3-dihydro-p-cumate + NAD+ [RN:R05247]
Reaction(KEGG)
R05247
Substrate
p-cumate [CPD:C06578];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(2R,3S)-2,3-dihydroxy-2,3-dihydro-p-cumate [CPD:C06579];
NAD+ [CPD:C00003]
Comment
The enzyme, characterized from several Pseudomonas strains, is involved in the degradation of p-cymene and p-cumate. It comprises four components: a ferredoxin, a ferredoxin reductase, and two subunits of a catalytic component. The enzyme can also act on indole, transforming it to the water-insoluble blue dye indigo.
History
EC 1.14.12.25 created 2016
Pathway
ec00622  Xylene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K10619  p-cumate 2,3-dioxygenase subunit alpha
K16303  p-cumate 2,3-dioxygenase subunit beta
Genes
RBDALSL_1087 ALSL_1088
PAEPA2083 PA2085
PAEVN297_2148 N297_2150
PAEIN296_2148 N296_2150
PAUPA14_37550 PA14_37570
PAGPLES_32361 PLES_32381
PAFPAM18_2958 PAM18_2960
PNCNCGM2_3063 NCGM2_3065
PAEBNCGM1900_4396 NCGM1900_4398
PDKPADK2_15225 PADK2_15235
PSGG655_14675 G655_14685
PRPM062_10830 M062_10840
PAEPPA1S_15255 PA1S_15265
PAEMU769_14855 U769_14865
PAELT223_16535 T223_16545
PAESSCV20265_3298 SCV20265_3300
PAEUBN889_02279 BN889_02281
PAEGAI22_18505 AI22_18515
PAECM802_2145 M802_2147
PAEOM801_2147 M801_2149
PPFPput_2897 Pput_2898
PPXT1E_4248(cbdC) T1E_4249
PMONX969_10845 X969_10850
PMOTX970_10500 X970_10505
PSESPSCI_1386 PSCI_1388
SPOIIMCC21906_02711 IMCC21906_02712
ZALAZF00_03880 AZF00_03885 AZF00_17855 AZF00_17860
TLOJ9253_01265 J9253_01270
GBIPG2T_01290 PG2T_01295 PG2T_14345 PG2T_14350
BXEBxe_A3555 Bxe_A3556
BXBDR64_1254 DR64_1255
PFIBPI93_021400 PI93_021405
BRKCWS35_25065 CWS35_25070
BARHWN72_35375 WN72_35380
RHZRHPLAN_11150 RHPLAN_11160 RHPLAN_44350 RHPLAN_44360
MONG8E03_15190 G8E03_15195
NREBES08_18565 BES08_18570
SMAZLH19_27420 LH19_27425
SWISwit_1757 Swit_1758
SPHDHY78_16795 HY78_16800
RDICMV14_03910 CMV14_03915
SPANAWL63_19960 AWL63_19965
SECHB18_22315 B18_22325
SPHJBSL82_10970 BSL82_10975
BACIB1NLA3E_13990 B1NLA3E_13995
OCNCUC15_03700 CUC15_03705
VIGBKP57_09420 BKP57_09425
LAOAOX59_08275 AOX59_08280
RUEDT065_15140 DT065_15145
SALEEPH95_15060 EPH95_15065
PBJVN24_05635 VN24_05640
PNPIJ22_32370 IJ22_32380
EFFskT53_01990
SPAEE2C16_04425 E2C16_04430
MMANMMAN_42610 MMAN_42620
MSAKMSAS_43620 MSAS_43630
MPAGC0J29_30955 C0J29_30960
MCOOMCOO_07710 MCOO_07720
MNED174_16895 D174_16905 D174_16915 D174_16920
MYNMyAD_16560 MyAD_16570 MyAD_16580 MyAD_16585
MHASMHAS_04025(bnzB) MHAS_04026(cbdA_2)
MANYMANY_01930 MANY_01940
ROPROP_46330(cmtA2) ROP_46340(cmtA1)
RRZCS378_10910 CS378_10915
RHODAOT96_33475 AOT96_33510
RRT4535765_00187(cmtA1_1) 4535765_00188(cmtA2_1) 4535765_00374(cmtA2_2) 4535765_00375(cmtA1_2)
RKOJWS14_14210 JWS14_14215
GPDGII33_08510 GII33_08525
SALSSLNWT_6906 SLNWT_6907
SCZABE83_01985 ABE83_01990
STSIA4E84_36025 A4E84_36030
SALWCP975_27200 CP975_27205 CP975_33970 CP975_33975
SBATG4Z16_01225
SHUNDWB77_04977(antA) DWB77_04978(hcaF)
PSIMKR76_24910 KR76_24915
FSYFsymDg_2033 FsymDg_2034
AMQAMETH_1199(benA) AMETH_1200
AACDLWP59_08660 LWP59_08665 LWP59_25375 LWP59_25380
PDXPsed_4287 Psed_4289
PSEQAD006_28390 AD006_28395 AD006_28760 AD006_28765
PECQAD017_28780 AD017_28785 AD017_29150 AD017_29155
PBROHOP40_30820 HOP40_30825
SSYIEKG83_16160 EKG83_16165
PMADBAY61_11485 BAY61_11490 BAY61_20660 BAY61_20690
MICHFJK98_14410 FJK98_14415
MCABHXZ27_11850 HXZ27_11855 HXZ27_13045 HXZ27_13050
VMAVAB18032_10630 VAB18032_10635
PRYPrubr_43520(hcaF) Prubr_43530
ATBJ4859_03360
EUZDVS28_a1943 DVS28_a1944 DVS28_a1989
TTLTtJL18_2435 TtJL18_2436
 » show all
Reference
1  [PMID:845117]
  Authors
DeFrank JJ, Ribbons DW
  Title
p-cymene pathway in Pseudomonas putida: initial reactions.
  Journal
J Bacteriol 129:1356-64 (1977)
DOI:10.1128/JB.129.3.1356-1364.1977
Reference
2  [PMID:7204334]
  Authors
Wigmore GJ, Ribbons DW
  Title
p-Cymene pathway in Pseudomonas putida: selective enrichment of defective mutants by using halogenated substrate analogs.
  Journal
J Bacteriol 143:816-24 (1980)
DOI:10.1128/JB.143.2.816-824.1980
Reference
3  [PMID:7592495]
  Authors
Eaton RW, Chapman PJ
  Title
Formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids.
  Journal
J Bacteriol 177:6983-8 (1995)
DOI:10.1128/JB.177.23.6983-6988.1995
Reference
4  [PMID:8631713]
  Authors
Eaton RW.
  Title
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon.
  Journal
J Bacteriol 178:1351-62 (1996)
DOI:10.1128/JB.178.5.1351-1362.1996
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.25
IUBMB Enzyme Nomenclature: 1.14.12.25
ExPASy - ENZYME nomenclature database: 1.14.12.25
BRENDA, the Enzyme Database: 1.14.12.25
LinkDB

KEGG   REACTION: R05247
Entry
R05247                      Reaction                               
Name
4-isopropylbenzoate:oxygen 2,3-oxidoreductase
Definition
p-Cumate + Oxygen + NADH + H+ <=> cis-2,3-Dihydroxy-2,3-dihydro-p-cumate + NAD+
Equation
Comment
p-cumate 2,3-dioxygenase
Reaction class
RC00001  C00003_C00004
RC00267  C06578_C06579
Enzyme
Pathway
rn00622  Xylene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01220  Degradation of aromatic compounds
Module
M00539  Cumate degradation, p-cumate => 2-oxopent-4-enoate + 2-methylpropanoate
Orthology
K10619  p-cumate 2,3-dioxygenase subunit alpha [EC:1.14.12.25]
K16303  p-cumate 2,3-dioxygenase subunit beta [EC:1.14.12.25]
K16304  p-cumate 2,3-dioxygenase ferredoxin component
K18227  p-cumate 2,3-dioxygenase ferredoxin reductase component [EC:1.18.1.3]
Other DBs
RHEA: 42347
LinkDB

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