KEGG ORTHOLOGY: K11395

ORTHOLOGY: K11395

Entry

K11395                      KO

Symbol

kdpgA

Name

2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase [EC:4.1.2.55]

Pathway

map00030

Pentose phosphate pathway

map00052

Galactose metabolism

map01100

Metabolic pathways

map01120

Microbial metabolism in diverse environments

map01200

Carbon metabolism

Module

M00309

Non-phosphorylative Entner-Doudoroff pathway, gluconate/galactonate => glycerate

M00633

Semi-phosphorylative Entner-Doudoroff pathway, gluconate/galactonate => glycerate-3P

Reaction

R01064

2-dehydro-3-deoxy-6-phospho-D-galactonate D-glyceraldehyde-3-phospho-lyase (pyruvate-forming)

R05605

2-dehydro-3-deoxy-6-phospho-D-gluconate D-glyceraldehyde-3-phosphate-lyase (pyruvate-forming)

R08570

2-dehydro-3-deoxy-D-gluconate D-glyceraldehyde-lyase (pyruvate-forming)

R10616

2-dehydro-3-deoxy-D-galactonate D-glyceraldehyde-lyase (pyruvate-forming)

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09101 Carbohydrate metabolism
   00030 Pentose phosphate pathway
    K11395  kdpgA; 2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase
   00052 Galactose metabolism
    K11395  kdpgA; 2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase
Enzymes [BR:ko01000]
4. Lyases
  4.1  Carbon-carbon lyases
   4.1.2  Aldehyde-lyases
    4.1.2.55  2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase
     K11395  kdpgA; 2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase

Other DBs

COG:

COG0329

GO:

0043725

Genes

TAC:

Ta0619

TVO:	TVG0663048(TVG0663048)

PTO:

PTO1026

FAC:	FACI_IFERC01G1727

FAI:

FAD_0700

STO:	STK_24790(kdgA)

SOH:	D1869_13570

SJV:	SJAV_08440

SSO:	SSO3197(eda)

SOL:

SSOA:

SSOL:

SSOF:

SSHI:

SCAS:

SIS:	LS215_2326

SIA:	M1425_2163

SIM:	M1627_2243

SID:

M164_2166

SIY:	YG5714_2289

SIN:	YN1551_0626

SII:

SIH:

SIR:

SIC:

SULA:

SULE:

SAI:

SACN:

SACR:

SACS:

SULO:

SULL:

MSE:

MEMJ:

MCN:

MHK:	DFR87_12295

MPRU:

DFR88_11840

MTEN:

GWK48_00445

MJN:	MjAS7_2363

AHO:

Ahos_0260

AMAN:

B6F84_04920

ABRI:

DFR85_02045 DFR85_14740

ASUL:

AAMB:

ACIH:

SACD:

SAZO:

CSTY:

STEP:

SMET:

SAHS:

PAS:

PYR:

POG:

TTN:	TTX_1156a(kdgA)

TUZ:

TUZN_0957

AG:

CAA11866

» show all

Reference

PMID:15869466

Authors

Ahmed H, Ettema TJ, Tjaden B, Geerling AC, van der Oost J, Siebers B

Title

The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation.

Journal

Biochem J 390:529-40 (2005)
DOI:10.1042/BJ20041711

Reference

PMID:17549431

Authors

Ettema TJ, Ahmed H, Geerling AC, van der Oost J, Siebers B

Title

The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) of Sulfolobus solfataricus: a key-enzyme of the semi-phosphorylative branch of the Entner-Doudoroff pathway.

Journal

Extremophiles 12:75-88 (2008)
DOI:10.1007/s00792-007-0082-1

Sequence

[sso:SSO3197]

LinkDB

ENZYME: 4.1.2.55

Entry

EC 4.1.2.55                 Enzyme

Name

2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase;
2-keto-3-deoxygluconate aldolase (ambiguous);
KDGA (ambiguous)

Class

Lyases;
Carbon-carbon lyases;
Aldehyde-lyases

Sysname

2-dehydro-3-deoxy-6-phospho-D-gluconate/2-dehydro-3-deoxy-6-phospho-D-galactonate D-glyceraldehyde-3-phosphate-lyase (pyruvate-forming)

Reaction(IUBMB)

(1) 2-dehydro-3-deoxy-6-phospho-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate [RN:R05605];
(2) 2-dehydro-3-deoxy-6-phospho-D-galactonate = pyruvate + D-glyceraldehyde 3-phosphate [RN:R01064]

Reaction(KEGG)

R01064 R05605;
(other) R08570 R10616

Substrate

2-dehydro-3-deoxy-6-phospho-D-gluconate [CPD:C04442];
2-dehydro-3-deoxy-6-phospho-D-galactonate [CPD:C01286]

Product

pyruvate [CPD:C00022];
D-glyceraldehyde 3-phosphate [CPD:C00118]

Comment

In the archaeon Sulfolobus solfataricus the enzyme is involved in glucose and galactose catabolism via the branched variant of the Entner-Doudoroff pathway. It utilizes 2-dehydro-3-deoxy-6-phosphate-D-gluconate and 2-dehydro-3-deoxy-6-phosphate-D-galactonate with similar catalytic efficiency. In vitro the enzyme can also catalyse the cleavage of the non-phosphorylated forms 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate with much lower catalytic efficiency. cf. EC 4.1.2.21, 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, and EC 4.1.2.14, 2-dehydro-3-deoxy-phosphogluconate aldolase.

History

EC 4.1.2.55 created 2014

Pathway

ec00030

Pentose phosphate pathway

ec00052

Galactose metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K11395

2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase

Genes

TAC:

Ta0619

TVO:	TVG0663048(TVG0663048)

PTO:

PTO1026

FAC:	FACI_IFERC01G1727

FAI:

FAD_0700

STO:	STK_24790(kdgA)

SOH:	D1869_13570

SJV:	SJAV_08440

SSO:	SSO3197(eda)

SOL:

Ssol_0934

SSOA:

SULA_0967

SSOL:

SULB_0969

SSOF:

SULC_0968

SSHI:

J5U23_00301

SCAS:

SACC_31320

SIS:	LS215_2326

SIA:	M1425_2163

SIM:	M1627_2243

SID:

M164_2166

SIY:	YG5714_2289

SIN:	YN1551_0626

SII:

LD85_2429

SIH:

SiH_2108

SIR:

SiRe_2040

SIC:

SiL_2015

SULA:

BFU36_06090

SULE:

GFS03_11780

SAI:

Saci_0225

SACN:

SacN8_01090

SACR:

SacRon12I_01090

SACS:

SUSAZ_01130

SULO:

GFS33_03590

SULL:

EWF20_14570

MSE:

Msed_1296

MEMJ:

MJ1HA_1503

MCN:

Mcup_0949

MHK:	DFR87_12295

MPRU:

DFR88_11840

MTEN:

GWK48_00445

MJN:	MjAS7_2363

AHO:

Ahos_0260

AMAN:

B6F84_04920

ABRI:

DFR85_02045 DFR85_14740

ASUL:

DFR86_04380

AAMB:

D1866_07690

ACIH:

HS5_15430

SACD:

HS1genome_1556

SAZO:

D1868_10845

CSTY:

KN1_14740

STEP:

IC006_2133

SMET:

RQ359_002107

SAHS:

HS7_04580

PAS:

Pars_1388

PYR:

P186_1933

POG:

Pogu_0825

TTN:	TTX_1156a(kdgA)

TUZ:

TUZN_0957

» show all

Reference

1 [PMID:10527934]

Authors

Buchanan CL, Connaris H, Danson MJ, Reeve CD, Hough DW

Title

An extremely thermostable aldolase from Sulfolobus solfataricus with specificity for non-phosphorylated substrates.

Journal

Biochem J 343 Pt 3:563-70 (1999)
DOI:10.1042/bj3430563

Sequence

[ag:CAA11866]

Reference

2 [PMID:16330030]

Authors

Lamble HJ, Theodossis A, Milburn CC, Taylor GL, Bull SD, Hough DW, Danson MJ

Title

Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus.

Journal

FEBS Lett 579:6865-9 (2005)
DOI:10.1016/j.febslet.2005.11.028

Sequence

[ag:CAA11866]

Reference

3 [PMID:17176250]

Authors

Wolterink-van Loo S, van Eerde A, Siemerink MA, Akerboom J, Dijkstra BW, van der Oost J

Title

Biochemical and structural exploration of the catalytic capacity of Sulfolobus KDG aldolases.

Journal

Biochem J 403:421-30 (2007)
DOI:10.1042/BJ20061419

Sequence

[sso:SSO3197] [sai:Saci_0225] [sto:STK_24790]

Other DBs

ExplorEnz - The Enzyme Database:

4.1.2.55

IUBMB Enzyme Nomenclature:

4.1.2.55

ExPASy - ENZYME nomenclature database:

4.1.2.55

BRENDA, the Enzyme Database:

4.1.2.55

LinkDB

REACTION: R01064

Entry

R01064                      Reaction

Name

2-dehydro-3-deoxy-6-phospho-D-galactonate D-glyceraldehyde-3-phospho-lyase (pyruvate-forming)

Definition

2-Dehydro-3-deoxy-6-phospho-D-galactonate <=> Pyruvate + D-Glyceraldehyde 3-phosphate

Equation

C01286 <=> C00022 + C00118

Reaction class

RC00307

C00022_C01286

RC00435

C00118_C01286

Enzyme

4.1.2.21 4.1.2.55

Pathway

rn00052

Galactose metabolism

rn01100

Metabolic pathways

Module

M00552

D-galactonate degradation, De Ley-Doudoroff pathway, D-galactonate => glycerate-3P

M00633

Semi-phosphorylative Entner-Doudoroff pathway, gluconate/galactonate => glycerate-3P

Brite

Enzymatic reactions [BR:br08201]
4. Lyase reactions
  4.1  Carbon-carbon lyases
   4.1.2  Aldehyde-lyases
    4.1.2.21
     R01064  2-Dehydro-3-deoxy-6-phospho-D-galactonate <=> Pyruvate + D-Glyceraldehyde 3-phosphate
    4.1.2.55
     R01064  2-Dehydro-3-deoxy-6-phospho-D-galactonate <=> Pyruvate + D-Glyceraldehyde 3-phosphate

Orthology

K01631

2-dehydro-3-deoxyphosphogalactonate aldolase [EC:4.1.2.21]

K11395

2-dehydro-3-deoxy-phosphogluconate/2-dehydro-3-deoxy-6-phosphogalactonate aldolase [EC:4.1.2.55]

Other DBs

RHEA:

24467

LinkDB

DBGET integrated database retrieval system