KEGG   ORTHOLOGY: K11821
Entry
K11821            Tight     KO                                     
Symbol
ST5A, SOT16
Name
aromatic desulfoglucosinolate sulfotransferase [EC:2.8.2.24]
Pathway
map00380  Tryptophan metabolism
map00966  Glucosinolate biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
map01210  2-Oxocarboxylic acid metabolism
Module
M00370  Glucosinolate biosynthesis, tryptophan => glucobrassicin
Reaction
R03214  3'-phosphoadenylylsulfate:desulfoglucosinolate sulfotransferase
R08167  3'-phosphoadenylyl-sulfate:indolylmethyl-desulfoglucosinolate sulfotransferase
R08662  3'-phosphoadenylyl-sulfate:desulfo-p-hydroxybenzylglucosinolate sulfotransferase
R08669  3'-phosphoadenylyl-sulfate:3-methylthiopropyl-desulfoglucosinolate sulfotransferase
R08671  3'-phosphoadenylyl-sulfate:4-methylthiobutyl-desulfoglucosinolate sulfotransferase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00380 Tryptophan metabolism
    K11821  ST5A, SOT16; aromatic desulfoglucosinolate sulfotransferase
  09110 Biosynthesis of other secondary metabolites
   00966 Glucosinolate biosynthesis
    K11821  ST5A, SOT16; aromatic desulfoglucosinolate sulfotransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.8  Transferring sulfur-containing groups
   2.8.2  Sulfotransferases
    2.8.2.24  aromatic desulfoglucosinolate sulfotransferase
     K11821  ST5A, SOT16; aromatic desulfoglucosinolate sulfotransferase
Other DBs
GO: 0047364
Genes
ATH: AT1G74100(SOT16)
ALY: 9323591
CRB: 17894300
CSAT: 104704865 104713044
EUS: EUTSA_v10018795mg
BRP: 103831884 103852881
BNA: 106354366 106381367 106415773 111199316
BOE: 106296616 106327830
RSZ: 108814527 108820205
Reference
PMID:19077143
  Authors
Klein M, Papenbrock J
  Title
Kinetics and substrate specificities of desulfo-glucosinolate sulfotransferases in Arabidopsis thaliana.
  Journal
Physiol Plant 135:140-9 (2009)
DOI:10.1111/j.1399-3054.2008.01182.x
  Sequence
[ath:AT1G74100]
Reference
PMID:16367753
  Authors
Klein M, Reichelt M, Gershenzon J, Papenbrock J
  Title
The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed.
  Journal
FEBS J 273:122-36 (2006)
DOI:10.1111/j.1742-4658.2005.05048.x
  Sequence
[ath:AT1G74100]
LinkDB

KEGG   ENZYME: 2.8.2.24
Entry
EC 2.8.2.24                 Enzyme                                 
Name
aromatic desulfoglucosinolate sulfotransferase;
desulfoglucosinolate sulfotransferase (ambiguous);
PAPS-desulfoglucosinolate sulfotransferase (ambiguous);
3'-phosphoadenosine-5'-phosphosulfate:desulfoglucosinolate sulfotransferase (ambiguous);
3'-phosphoadenylyl-sulfate:aromatic desulfoglucosinolate sulfotransferase
Class
Transferases;
Transferring sulfur-containing groups;
Sulfotransferases
Sysname
3'-phosphoadenylyl-sulfate:aromatic desulfoglucosinolate sulfonotransferase
Reaction(IUBMB)
(1) 3'-phosphoadenylyl sulfate + desulfoglucotropeolin = adenosine 3',5'-bisphosphate + glucotropeolin [RN:R03214];
(2) 3'-phosphoadenylyl sulfate + indolylmethyl-desulfoglucosinolate = adenosine 3',5'-bisphosphate + glucobrassicin [RN:R08167]
Reaction(KEGG)
R03214 R08167
Substrate
3'-phosphoadenylyl sulfate [CPD:C00053];
desulfoglucotropeolin [CPD:C01069];
indolylmethyl-desulfoglucosinolate [CPD:C16517]
Product
adenosine 3',5'-bisphosphate [CPD:C00054];
glucotropeolin [CPD:C02153];
glucobrassicin [CPD:C05837]
Comment
This enzyme, characterized from cruciferous plants, catalyses the last step in the biosynthesis of tryptophan- and phenylalanine-derived glucosinolates. cf. EC 2.8.2.38, aliphatic desulfoglucosinolate sulfotransferase.
History
EC 2.8.2.24 created 1992, modified 2017
Pathway
ec00380  Tryptophan metabolism
ec00966  Glucosinolate biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K11821  aromatic desulfoglucosinolate sulfotransferase
Genes
ATH: AT1G74100(SOT16)
ALY: 9323591
CRB: 17894300
CSAT: 104704865 104713044
EUS: EUTSA_v10018795mg
BRP: 103831884 103852881
BNA: 106354366 106381367 106415773 111199316
BOE: 106296616 106327830
RSZ: 108814527 108820205
Reference
1  [PMID:2524977]
  Authors
Jain JC, Reed DW, GrootWassink JW, Underhill EW.
  Title
A radioassay of enzymes catalyzing the glucosylation and sulfation steps of glucosinolate biosynthesis in Brassica species.
  Journal
Anal Biochem 178:137-40 (1989)
DOI:10.1016/0003-2697(89)90369-2
Reference
2  [PMID:16367753]
  Authors
Klein M, Reichelt M, Gershenzon J, Papenbrock J
  Title
The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed.
  Journal
FEBS J 273:122-36 (2006)
DOI:10.1111/j.1742-4658.2005.05048.x
  Sequence
[ath:AT1G74100]
Reference
3  [PMID:19077143]
  Authors
Klein M, Papenbrock J
  Title
Kinetics and substrate specificities of desulfo-glucosinolate sulfotransferases in Arabidopsis thaliana.
  Journal
Physiol Plant 135:140-9 (2009)
DOI:10.1111/j.1399-3054.2008.01182.x
  Sequence
[ath:AT1G74100]
Other DBs
ExplorEnz - The Enzyme Database: 2.8.2.24
IUBMB Enzyme Nomenclature: 2.8.2.24
ExPASy - ENZYME nomenclature database: 2.8.2.24
BRENDA, the Enzyme Database: 2.8.2.24
CAS: 121479-85-4
LinkDB

KEGG   REACTION: R08167
Entry
R08167                      Reaction                               
Name
3'-phosphoadenylyl-sulfate:indolylmethyl-desulfoglucosinolate sulfotransferase
Definition
Indolylmethyl-desulfoglucosinolate + 3'-Phosphoadenylyl sulfate <=> Glucobrassicin + Adenosine 3',5'-bisphosphate
Equation
C16517 + C00053 <=> C05837 + C00054
Reaction class
RC00007  C00053_C00054
RC00883  C05837_C16517
Enzyme
2.8.2.24
Pathway
rn00380  Tryptophan metabolism
rn00966  Glucosinolate biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
rn01210  2-Oxocarboxylic acid metabolism
Module
M00370  Glucosinolate biosynthesis, tryptophan => glucobrassicin
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.8  Transferring sulfur-containing groups
   2.8.2  Sulfotransferases
    2.8.2.24
     R08167  Indolylmethyl-desulfoglucosinolate + 3'-Phosphoadenylyl sulfate <=> Glucobrassicin + Adenosine 3',5'-bisphosphate
Orthology
K11821  aromatic desulfoglucosinolate sulfotransferase [EC:2.8.2.24]
Other DBs
RHEA: 52739
LinkDB

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