Entry
Name
L-cysteine---[L-cysteinyl-carrier protein] ligase;
pchE (gene name);
pchF (gene name);
angR (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
L-cysteine:[L-cysteinyl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-cysteine + holo-[L-cysteinyl-carrier protein] = AMP + diphosphate + L-cysteinyl-[L-cysteinyl-carrier protein] (overall reaction);
(1a) ATP + L-cysteine = diphosphate + (L-cysteinyl)adenylate [RN:
R12765 ];
(1b) (L-cysteinyl)adenylate + holo-[L-cysteinyl-carrier protein] = AMP + L-cysteinyl-[L-cysteinyl-carrier protein]
Reaction(KEGG)
Substrate
ATP [CPD:
C00002 ];
L-cysteine [CPD:
C00097 ];
holo-[L-cysteinyl-carrier protein];
(L-cysteinyl)adenylate [CPD:
C22385 ]
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
L-cysteinyl-[L-cysteinyl-carrier protein];
(L-cysteinyl)adenylate [CPD:
C22385 ]
Comment
The adenylation domain of the enzyme catalyses the activation of L-cysteine to (L-cysteinyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of a peptidyl-carrier protein domain. The peptidyl-carrier protein domain may be part of the same protein, or of a different protein. This activity is often found as part of a larger non-ribosomal peptide synthase.
History
EC 6.2.1.69 created 2021
Pathway
ec01053 Biosynthesis of siderophore group nonribosomal peptides
Orthology
K12239 L-cysteine---[L-cysteinyl-carrier protein] ligase PchE
K12240 L-cysteine---[L-cysteinyl-carrier protein] ligase PchF
Genes
PGE : LG71_13430 LG71_26195
PAE : PA4225(pchF) PA4226(pchE)
PAEV : N297_4357 N297_4358
PAEI : N296_4357 N296_4358
PAU : PA14_09270(pchE) PA14_09280(pchF) PA14_54920
PAP : PSPA7_0874(pchE) PSPA7_0875(pchF)
PAG : PLES_07011(pchE) PLES_07021(pchF)
PAF : PAM18_0711(pchE) PAM18_0712(pchF)
PNC : NCGM2_5470(pchF) NCGM2_5471(pchE)
PAEB : NCGM1900_0733(pchE) NCGM1900_0734(pchF)
PDK : PADK2_03540 PADK2_03545
PSG : G655_03575 G655_03580
PRP : M062_22260 M062_22265
PAEP : PA1S_03690 PA1S_03695
PAER : PA1R_gp2116 PA1R_gp2117
PAEM : U769_03720 U769_03725
PAEL : T223_03620 T223_03625
PAES : SCV20265_0735 SCV20265_0736
PAEU : BN889_04699(pchF) BN889_04700(pchE_1) BN889_04701(pchE_2)
PAEG : AI22_00215 AI22_00220
PAEC : M802_4355 M802_4356
PAEO : M801_4223 M801_4224
SECH : B18_04270 B18_04275
PFL : PFL_3492(pchF) PFL_3493(pchE)
PPRC : PFLCHA0_c35330(irp1) PFLCHA0_c35340(irp2)
PPRO : PPC_3646(pchF) PPC_3647(pchE)
PVR : PverR02_15520 PverR02_15525
PCZ : PCL1606_27860 PCL1606_27870
PSEM : TO66_16985 TO66_16990
PSOS : POS17_3635(pchF) POS17_3636(pchE)
PSEP : C4K39_5485 C4K39_5486
PSNC : C0058_16995 C0058_17000
SAJS : QO259_16200 QO259_16205
BPS : BPSS0586(pchE) BPSS0587(pchF)
BPM : BURPS1710b_A2150(pchE) BURPS1710b_A2151(pchF)
BPL : BURPS1106A_A0787 BURPS1106A_A0788
BPD : BURPS668_A0877 BURPS668_A0878
BPZ : BP1026B_II0646(pchE) BP1026B_II0647(pchF)
BPQ : BPC006_II0832 BPC006_II0833
BPSH : DR55_4763 DR55_4764
BPSO : X996_3767 X996_3768
BTE : BTH_II1827 BTH_II1828(pchE)
BTHM : BTRA_3856 BTRA_3857
BTHA : DR62_4285 DR62_4286
BTHL : BG87_4568 BG87_4569
BUR : Bcep18194_B0672 Bcep18194_B0673
BCM : Bcenmc03_5289 Bcenmc03_5290
BCH : Bcen2424_4997 Bcen2424_4998
BCEO : I35_6120(pchF) I35_6121(pchE)
BCED : DM42_5764 DM42_5765
BCEP : APZ15_27910 APZ15_27915
BSEM : WJ12_31050 WJ12_31055
BSTL : BBJ41_24870 BBJ41_24875
BARI : NLX30_26105 NLX30_26110
BSER : R0290_17830 R0290_17835
BGP : BGL_1c20570(pchF) BGL_1c20580(pchE)
BPLA : bpln_1g19090 bpln_1g19100
ALI : AZOLI_p20160(pchE) AZOLI_p20161(pchF)
CAUN : CLAUR_026160(dltA_6)
RPAY : P0092_11085 P0092_11095
BPRO : PMF13cell1_00657(mbtB_1)
MANA : MAMMFC1_02828(mbtB_2)
STED : SPTER_12030(mbtB_1)
SSPH : SPSPH_007900(dltA_1) SPSPH_007940(dltA_2)
EYY : EGYY_00410(EntF) EGYY_29280(EntF)
CDD : CDCE8392_1964(irp2C)
CDIP : ERS451417_02094(irp2C)
NFR : ERS450000_00763(mbtB_1)
NIE : KV110_22875 KV110_22880 KV110_35845
SRC : M271_09930 M271_09960
SGU : SGLAU_04465(nrps1B) SGLAU_04470(nrps1C) SGLAU_04640(nrps1D) SGLAU_04650(nrps1E)
SPHW : NFX46_19160 NFX46_19185
SPAD : DVK44_09635 DVK44_09640
SHUN : DWB77_06824(mbtB_1)
DCO : SAMEA4475696_1592(mbtB_3)
PAUS : NCTC13651_00740(mbtB_1)
AORI : SD37_07330 SD37_10150
AMYZ : HUW46_08246(dltA_17)
ASE : ACPL_6139(nrps2A) ACPL_6158(nrps2C)
ACTS : ACWT_6007 ACWT_6026
AOU : ACTOB_004298 ACTOB_004317
FCG : LNP23_13845 LNP23_13860
AQR : HN014_08650 HN014_08670
» show all
Taxonomy
Reference
Authors
Quadri LE, Keating TA, Patel HM, Walsh CT
Title
Assembly of the Pseudomonas aeruginosa nonribosomal peptide siderophore pyochelin: In vitro reconstitution of aryl-4, 2-bisthiazoline synthetase activity from PchD, PchE, and PchF.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.69
ExPASy - ENZYME nomenclature database: 6.2.1.69
LinkDB
All DBs