KEGG ORTHOLOGY: K12635

ORTHOLOGY: K12635

Entry

K12635            Tight     KO

Symbol

dmpM

Name

O-demethylpuromycin O-methyltransferase [EC:2.1.1.38]

Pathway

map00998

Biosynthesis of various antibiotics

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Module

M00889

Puromycin biosynthesis, ATP => puromycin

Reaction

R05676

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00998 Biosynthesis of various antibiotics
    K12635  dmpM; O-demethylpuromycin O-methyltransferase
Enzymes [BR:ko01000]
2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.38  O-demethylpuromycin O-methyltransferase
     K12635  dmpM; O-demethylpuromycin O-methyltransferase

Other DBs

GO:

0030739

Genes

SALW:

CP975_29310

AG:	AAB00531(dmpM)

Reference

PMID:1756982

Authors

Lacalle RA, Ruiz D, Jimenez A

Title

Molecular analysis of the dmpM gene encoding an O-demethyl puromycin O-methyltransferase from Streptomyces alboniger.

Journal

Gene 109:55-61 (1991)
DOI:10.1016/0378-1119(91)90588-3

Sequence

[ag:AAB00531]

LinkDB

ENZYME: 2.1.1.38

Entry

EC 2.1.1.38                 Enzyme

Name

O-demethylpuromycin O-methyltransferase;
O-demethylpuromycin methyltransferase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:O-demethylpuromycin O-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + O-demethylpuromycin = S-adenosyl-L-homocysteine + puromycin [RN:R03633]

Reaction(KEGG)

R03633;
(other) R05676

Substrate

S-adenosyl-L-methionine [CPD:C00019];
O-demethylpuromycin [CPD:C02864]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
puromycin [CPD:C01610]

Comment

Puromycin is the antibiotic derived from N6-dimethyladenosine by replacing the 3'-hydroxy group with an amino group and acylating this with 4-O-methyltyrosine.

History

EC 2.1.1.38 created 1972

Pathway

ec00998

Biosynthesis of various antibiotics

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K12635

O-demethylpuromycin O-methyltransferase

Genes

SALW:

CP975_29310

Reference

1 [PMID:5773275]

Authors

Rao MM, Rebello PF, Pogell BM.

Title

Biosynthesis of puromycin in Streptomyces alboniger. Enzymatic methylation of O-demethylpuromycin.

Journal

J Biol Chem 244:112-8 (1969)

Sequence

[ag:AAB00531]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.38

IUBMB Enzyme Nomenclature:

2.1.1.38

ExPASy - ENZYME nomenclature database:

2.1.1.38

BRENDA, the Enzyme Database:

2.1.1.38

CAS:	37257-04-8

LinkDB

REACTION: R05676

Entry

R05676                      Reaction

Definition

N-Acetyl-O-demethylpuromycin + S-Adenosyl-L-methionine <=> N-Acetylpuromycin + S-Adenosyl-L-homocysteine

Equation

C07031 + C00019 <=> C07032 + C00021

Comment

DmpM
Ref.1. The Journal of Biological chemistry; vol.271, No.3, Jan 19,
pp.1579-1590, 1996

Reaction class

RC00003

C00019_C00021

RC00392

C07031_C07032

Enzyme

2.1.1.38

Pathway

rn00998

Biosynthesis of various antibiotics

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

Module

M00889

Puromycin biosynthesis, ATP => puromycin

Brite

Enzymatic reactions [BR:br08201]
2. Transferase reactions
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.38
     R05676  N-Acetyl-O-demethylpuromycin + S-Adenosyl-L-methionine <=> N-Acetylpuromycin + S-Adenosyl-L-homocysteine

Orthology

K12635

O-demethylpuromycin O-methyltransferase [EC:2.1.1.38]

LinkDB

DBGET integrated database retrieval system