KEGG   ORTHOLOGY: K12637
Entry
K12637                      KO                                     
Symbol
CYP90C1, ROT3
Name
3-epi-6-deoxocathasterone 23-monooxygenase [EC:1.14.14.147]
Pathway
map00905  Brassinosteroid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00371  Castasterone biosynthesis, campesterol => castasterone
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00905 Brassinosteroid biosynthesis
    K12637  CYP90C1, ROT3; 3-epi-6-deoxocathasterone 23-monooxygenase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K12637  CYP90C1, ROT3; 3-epi-6-deoxocathasterone 23-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.147  22alpha-hydroxysteroid 23-monooxygenase
     K12637  CYP90C1, ROT3; 3-epi-6-deoxocathasterone 23-monooxygenase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP90 family
   K12637  CYP90C1, ROT3; 3-epi-6-deoxocathasterone 23-monooxygenase
Other DBs
RN: R07431 R07448 R07786 R07787 R07791 R07792
Genes
ATH: AT4G36380(ROT3)
ALY: 9303091
CRB: 17877976
CSAT: 104716496(ROT3) 104721136 104733119
EUS: EUTSA_v10024901mg
BRP: 103862467 103868633
BNA: 106369862 106409581 106451959
BOE: 106302041 106307279
RSZ: 108839589 108849049
THJ: 104807476
CPAP: 110822476
CIT: 102623590
PVY: 116118949
TCC: 18610843
EGR: 104422732
LJA: Lj2g3v1728770.1(Lj2g3v1728770.1) Lj2g3v1728770.2(Lj2g3v1728770.2)
ADU: 107488015
AIP: 107642922
LANG: 109348710
FVE: 101302700
RCN: 112188662
PPER: 18771246
PMUM: 103340160
PAVI: 110773923
PDUL: 117636128
ZJU: 107424559
MNT: 112094164
CSV: 101203911
CMO: 103485087
BHJ: 120073232
MCHA: 111005423
CMAX: 111496682
CMOS: 111463512
CPEP: 111782027
RCU: 8279118
JCU: 105639077
MESC: 110608590
POP: 7495513
PEU: 105126648
PALZ: 118063091
JRE: 108992064
TWL: 120013070
VVI: 100259060
VRI: 117912322
SLY: 101245605
SPEN: 107009038
SOT: 102598106
SSTN: 125849542
CANN: 107860019
NSY: 104213597
NTO: 104118978
NAU: 109210485
INI: 109177767
ITR: 116000340
SIND: 105165748
EGT: 105965330
HAN: 110915370
ECAD: 122579553
CCAV: 112513182
DCR: 108219828
CSIN: 114279029
BVG: 104895094
SOE: 110798894
MING: 122074479
TSS: 122661980
PDA: 103723970
EGU: 105042258
MUS: 103995745
AOF: 109835455
 » show all
Reference
  Authors
Ohnishi T, Szatmari AM, Watanabe B, Fujita S, Bancos S, Koncz C, Lafos M, Shibata K, Yokota T, Sakata K, Szekeres M, Mizutani M
  Title
C-23 hydroxylation by Arabidopsis CYP90C1 and CYP90D1 reveals a novel shortcut in brassinosteroid biosynthesis.
  Journal
Plant Cell 18:3275-88 (2006)
DOI:10.1105/tpc.106.045443
  Sequence
[ath:AT4G36380]
LinkDB

KEGG   ORTHOLOGY: K12638
Entry
K12638                      KO                                     
Symbol
CYP90D1
Name
3-epi-6-deoxocathasterone 23-monooxygenase [EC:1.14.14.147]
Pathway
map00905  Brassinosteroid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00371  Castasterone biosynthesis, campesterol => castasterone
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00905 Brassinosteroid biosynthesis
    K12638  CYP90D1; 3-epi-6-deoxocathasterone 23-monooxygenase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K12638  CYP90D1; 3-epi-6-deoxocathasterone 23-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.147  22alpha-hydroxysteroid 23-monooxygenase
     K12638  CYP90D1; 3-epi-6-deoxocathasterone 23-monooxygenase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP90 family
   K12638  CYP90D1; 3-epi-6-deoxocathasterone 23-monooxygenase
Other DBs
RN: R07431 R07448 R07786 R07787 R07791 R07792
Genes
ATH: AT3G13730(CYP90D1)
ALY: 9298835 9321051
CRB: 17893533
CSAT: 104745632 104765165
EUS: EUTSA_v10020591mg
BRP: 103842602 103870101
BNA: 106350972 106351089 106426522 106440615 106452300
BOE: 106327160 106344076
RSZ: 108805921 108857636
THJ: 104810577
CPAP: 110818395
CIT: 102610008
PVY: 116106176
TCC: 18596114
GRA: 105769910
GAB: 108451933
EGR: 104449963
VRA: 106773857
VAR: 108323332
VUN: 114166334
CCAJ: 109809062
CAM: 101505609
LJA: Lj3g3v2693130.1(Lj3g3v2693130.1) Lj3g3v2693130.2(Lj3g3v2693130.2)
LANG: 109358278
FVE: 101297721
RCN: 112187971
PPER: 18784338
PMUM: 103327717
PAVI: 110767804
PDUL: 117622280
MDM: 103453669
ZJU: 107403831
MNT: 21388039
MCHA: 111016959
CMAX: 111479936
CMOS: 111458064
CPEP: 111790258
RCU: 8266971
JCU: 105631477
HBR: 110648648
MESC: 110626427
QSU: 112003250
QLO: 115989007
TWL: 120015527
VVI: 100259945
VRI: 117921515
SLY: 101256170
SPEN: 107014078
SOT: 102582597
SSTN: 125859968
CANN: 107861744
NSY: 104213362
NTO: 104121493
NAU: 109231818
INI: 109190236
ITR: 116023130
SIND: 105157742
OEU: 111367697
EGT: 105975773
ECAD: 122596266
LSV: 111910313
CCAV: 112517254
DCR: 108220552
BVG: 104900369
SOE: 110785866
NNU: 104586612
AOF: 109828140
SMO: SELMODRAFT_151754(ROT3B-1) SELMODRAFT_157387(CYP90E3) SELMODRAFT_182839(ROT3A-2)
 » show all
Reference
  Authors
Ohnishi T, Szatmari AM, Watanabe B, Fujita S, Bancos S, Koncz C, Lafos M, Shibata K, Yokota T, Sakata K, Szekeres M, Mizutani M
  Title
C-23 hydroxylation by Arabidopsis CYP90C1 and CYP90D1 reveals a novel shortcut in brassinosteroid biosynthesis.
  Journal
Plant Cell 18:3275-88 (2006)
DOI:10.1105/tpc.106.045443
  Sequence
[ath:AT3G13730]
LinkDB

KEGG   ENZYME: 1.14.14.147
Entry
EC 1.14.14.147              Enzyme                                 
Name
22alpha-hydroxysteroid 23-monooxygenase;
cytochrome P450 90C1;
CYP90D1;
CYP90C1;
3-epi-6-deoxocathasterone,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (C-23-hydroxylating);
3-epi-6-deoxocathasterone 23-monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
22alpha-hydroxysteroid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (C-23-hydroxylating)
Reaction(IUBMB)
(1) 3-epi-6-deoxocathasterone + [reduced NADPH---hemoprotein reductase] + O2 = 6-deoxotyphasterol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07792];
(2) (22S,24R)-22-hydroxy-5alpha-ergostan-3-one + [reduced NADPH---hemoprotein reductase] + O2 = 3-dehydro-6-deoxoteasterone + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07791]
Reaction(KEGG)
R07791 R07792;
(other) R07786 R07787
Substrate
3-epi-6-deoxocathasterone [CPD:C16251];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
(22S,24R)-22-hydroxy-5alpha-ergostan-3-one [CPD:C15797]
Product
6-deoxotyphasterol [CPD:C15801];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
3-dehydro-6-deoxoteasterone [CPD:C15800]
Comment
A cytochrome P-450 (heme-thiolate) protein involved in brassinosteroid biosynthesis in plants. The enzyme has a relaxed substrate specificity, and C-23 hydroxylation can occur at different stages in the pathway. In Arabidopsis thaliana two isozymes, encoded by the CYP90C1 and CYP90D1 genes, have redundant activities.
History
EC 1.14.14.147 created 2010 as EC 1.14.13.112, transferred 2018 to EC 1.14.14.147, modified 2022
Pathway
ec00905  Brassinosteroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12637  3-epi-6-deoxocathasterone 23-monooxygenase
K12638  3-epi-6-deoxocathasterone 23-monooxygenase
Genes
ATHAT3G13730(CYP90D1) AT4G36380(ROT3)
ALY9298835 9303091 9321051
CRB17877976 17893533
CSAT104716496(ROT3) 104721136 104733119 104745632 104765165
EUSEUTSA_v10020591mg EUTSA_v10024901mg
BRP103842602 103862467 103868633 103870101
BNA106350972 106351089 106369862 106409581 106426522 106440615 106451959 106452300
BOE106302041 106307279 106327160 106344076
RSZ108805921 108839589 108849049 108857636
THJ104807476 104810577
CPAP110818395 110822476
CIT102610008 102623590
CICCICLE_v10025448mg CICLE_v10025479mg
PVY116106176 116118949
MINC123198912 123205796 123219507 123219819
TCC18596114 18610843
GRA105769910 105787502 105803759
GHI107906110 107913036 107936555 107943285 121209822
GAB108451933 108458395 108472765
DZI111274623 111280433 111293281 111301082 111316248
EGR104422732 104449963
GMX100782687 100797868 100799030 100812489
GSJ114375691 114381782 114417944 114420172 114422309
PVUPHAVU_002G167400g PHAVU_003G258400g PHAVU_010G139600g
VRA106768728 106773857 106777471
VAR108323332 108335338 108336674
VUN114166334 114174236 114179716
CCAJ109801110 109809062 109814118
APRC113851021 113856000 113858772 113870295
MTRMTR_5g021220 MTR_8g016180 MTR_8g076510
CAM101488381 101505609 101509993
LJALj2g3v1728770.1(Lj2g3v1728770.1) Lj2g3v1728770.2(Lj2g3v1728770.2) Lj3g3v2693130.1(Lj3g3v2693130.1) Lj3g3v2693130.2(Lj3g3v2693130.2)
ADU107461434 107464245 107488015
AIP107612705 107618558 107642922
AHF112706501 112710103 112749491 112772650 112777343 112800770
LANG109348710 109358278
FVE101297721 101302700
RCN112187971 112188662
PPER18771246 18784338
PMUM103327717 103340160
PAVI110767804 110773923
PDUL117622280 117636128
MDM103400874 103431557 103453669
PXB103934102 103957186
ZJU107403831 107424559
MNT112094164 21388039
CSV101203911 101212581 101217830
CMO103485087 103490016 103500734
BHJ120073232 120086051 120091319
MCHA111005423 111016959
CMAX111479936 111496682
CMOS111458064 111463512
CPEP111782027 111790258
RCU8266971 8279118
JCU105631477 105639077
HBR110639585 110648648 110668522
MESC110608590 110626427
POP112326932 18096744 7465155 7495513
PEU105114341 105118255 105118442 105126648
PALZ118030995 118040884 118063091
JRE108991288 108992064 109007914
QSU111995943 112000874 112003250
QLO115962687 115989007 115994450
TWL120013070 120015527
VVI100259060 100259945
VRI117912322 117921515
SLY101245605 101256170
SPEN107009038 107014078
SOT102582597 102598106
SSTN125849542 125859968
CANN107860019 107861744
NTA107771928 107795325 107795780 107816861
NSY104213362 104213597
NTO104118978 104121493
NAU109210485 109231818
INI109177767 109190236
ITR116000340 116023130
SIND105157742 105165748
OEU111367697
EGT105965330 105975773
SSPL121767418 121768407 121771701 121771730
HAN110886538 110894209 110915370
ECAD122579553 122596266
LSV111897310 111910313 122194394
CCAV112513182 112517254
DCR108219828 108220552
CSIN114270454 114279029 114309759
BVG104895094 104900369
SOE110785866 110798894
CQI110682556 110684296 110685861 110719834
NNU104586612 104595594 104610557
MING122074479 122078744 122089610
TSS122653626 122661980 122667105
PSOM113288714 113288788 113347873 113349511
NCOL116256449 116256903
PDA103712873 103718648 103723970
EGU105040406 105042258 105047955 105059996
MUS103969391 103975346 103991264 103995745 103999307
DCT110094797 110107619
PEQ110020222 110022604
AOF109828140 109835455
ATR18429333 18448141
SMOSELMODRAFT_151754(ROT3B-1) SELMODRAFT_157387(CYP90E3) SELMODRAFT_182839(ROT3A-2)
 » show all
Reference
1  [PMID:15703058]
  Authors
Kim GT, Fujioka S, Kozuka T, Tax FE, Takatsuto S, Yoshida S, Tsukaya H.
  Title
CYP90C1 and CYP90D1 are involved in different steps in the brassinosteroid biosynthesis pathway in Arabidopsis thaliana.
  Journal
Plant J 41:710-21 (2005)
DOI:10.1111/j.1365-313X.2004.02330.x
Reference
2  [PMID:17138693]
  Authors
Ohnishi T, Szatmari AM, Watanabe B, Fujita S, Bancos S, Koncz C, Lafos M, Shibata K, Yokota T, Sakata K, Szekeres M, Mizutani M
  Title
C-23 hydroxylation by Arabidopsis CYP90C1 and CYP90D1 reveals a novel shortcut in brassinosteroid biosynthesis.
  Journal
Plant Cell 18:3275-88 (2006)
DOI:10.1105/tpc.106.045443
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.147
IUBMB Enzyme Nomenclature: 1.14.14.147
ExPASy - ENZYME nomenclature database: 1.14.14.147
BRENDA, the Enzyme Database: 1.14.14.147
LinkDB

KEGG   REACTION: R07787
Entry
R07787                      Reaction                               
Name
22alpha-hydroxy-campest-4-en-3-one,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (C-23-hydroxylating)
Definition
(22S)-22-Hydroxycampest-4-en-3-one + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> (22R,23R)-22,23-Dihydroxy-campest-4-en-3-one + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
Reaction class
RC02078  C15796_C16253
Enzyme
Pathway
rn00905  Brassinosteroid biosynthesis
Orthology
K12637  3-epi-6-deoxocathasterone 23-monooxygenase [EC:1.14.14.147]
K12638  3-epi-6-deoxocathasterone 23-monooxygenase [EC:1.14.14.147]
Other DBs
RHEA: 69826
LinkDB

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