KEGG   ORTHOLOGY: K12705
Entry
K12705            Tight     KO                                     
Symbol
novO, couO
Name
8-demethylnovobiocic acid C8-methyltransferase [EC:2.1.1.284]
Pathway
map00401  Novobiocin biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Reaction
R06778  
R10454  S-adenosyl-L-methionine:8-demethylnovobiocic acid C8-methyltransferase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00401 Novobiocin biosynthesis
    K12705  novO, couO; 8-demethylnovobiocic acid C8-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.284  8-demethylnovobiocic acid C8-methyltransferase
     K12705  novO, couO; 8-demethylnovobiocic acid C8-methyltransferase
Genes
AG: AAF67508(novO) AAG29793(couO)
Reference
PMID:10770754
  Authors
Steffensky M, Muhlenweg A, Wang ZX, Li SM, Heide L
  Title
Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891.
  Journal
Antimicrob Agents Chemother 44:1214-22 (2000)
DOI:10.1128/AAC.44.5.1214-1222.2000
  Sequence
[ag:AAF67508]
Reference
PMID:18663394
  Authors
Sattely ES, Fischbach MA, Walsh CT
  Title
Total biosynthesis: in vitro reconstitution of polyketide and nonribosomal peptide pathways.
  Journal
Nat Prod Rep 25:757-93 (2008)
DOI:10.1039/b801747f
LinkDB

KEGG   ENZYME: 2.1.1.284
Entry
EC 2.1.1.284                Enzyme                                 
Name
8-demethylnovobiocic acid C8-methyltransferase;
NovO
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:8-demethylnovobiocic acid C8-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + 8-demethylnovobiocic acid = S-adenosyl-L-homocysteine + novobiocic acid [RN:R10454]
Reaction(KEGG)
R10454
Substrate
S-adenosyl-L-methionine [CPD:C00019];
8-demethylnovobiocic acid [CPD:C20629]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
novobiocic acid [CPD:C12474]
Comment
The enzyme is involved in the biosynthesis of the aminocoumarin antibiotic novobiocin.
History
EC 2.1.1.284 created 2013
Pathway
ec00401  Novobiocin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12705  8-demethylnovobiocic acid C8-methyltransferase
Reference
1  [PMID:16274243]
  Authors
Pacholec M, Tao J, Walsh CT
  Title
CouO and NovO: C-methyltransferases for tailoring the aminocoumarin scaffold in coumermycin and novobiocin antibiotic biosynthesis.
  Journal
Biochemistry 44:14969-76 (2005)
DOI:10.1021/bi051599o
  Sequence
[ag:AAF67508]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.284
IUBMB Enzyme Nomenclature: 2.1.1.284
ExPASy - ENZYME nomenclature database: 2.1.1.284
BRENDA, the Enzyme Database: 2.1.1.284
LinkDB

KEGG   REACTION: R10454
Entry
R10454                      Reaction                               
Name
S-adenosyl-L-methionine:8-demethylnovobiocic acid C8-methyltransferase
Definition
S-Adenosyl-L-methionine + 8-Demethylnovobiocic acid <=> S-Adenosyl-L-homocysteine + Novobiocic acid
Equation
C00019 + C20629 <=> C00021 + C12474
Reaction class
RC00003  C00019_C00021
RC01662  C12474_C20629
Enzyme
2.1.1.284
Pathway
rn00401  Novobiocin biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.284
     R10454  S-Adenosyl-L-methionine + 8-Demethylnovobiocic acid <=> S-Adenosyl-L-homocysteine + Novobiocic acid
Orthology
K12705  8-demethylnovobiocic acid C8-methyltransferase [EC:2.1.1.284]
Other DBs
RHEA: 36654
LinkDB

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