KEGG   ORTHOLOGY: K12708
Entry
K12708            Tight     KO                                     
Symbol
novR, cloR
Name
4-hydroxy-3-prenylphenylpyruvate oxygenase / 4-hydroxy-3-prenylbenzoate synthase [EC:1.13.11.83 1.13.12.23]
Pathway
map00401  Novobiocin biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R06758  3-(4-hydroxy-3-prenylphenyl)pyruvate:oxygen 1,2-oxidoreductase (4-hydroxy-3-prenylmandelate-forming)
R06759  4-hydroxy-3-prenylmandelate:oxygen oxidoreductase (4-hydroxy-3-prenylbenzoate forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00401 Novobiocin biosynthesis
    K12708  novR, cloR; 4-hydroxy-3-prenylphenylpyruvate oxygenase / 4-hydroxy-3-prenylbenzoate synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.13  Acting on single donors with incorporation of molecular oxygen (oxygenases)
   1.13.11  With incorporation of two atoms of oxygen
    1.13.11.83  4-hydroxy-3-prenylphenylpyruvate oxygenase
     K12708  novR, cloR; 4-hydroxy-3-prenylphenylpyruvate oxygenase / 4-hydroxy-3-prenylbenzoate synthase
   1.13.12  With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
    1.13.12.23  4-hydroxy-3-prenylbenzoate synthase
     K12708  novR, cloR; 4-hydroxy-3-prenylphenylpyruvate oxygenase / 4-hydroxy-3-prenylbenzoate synthase
Other DBs
COG: COG0235
Genes
NGV: CDO52_02235
AG: AAF67511(novR) AAN65240(cloR)
Reference
PMID:12777382
  Authors
Pojer F, Kahlich R, Kammerer B, Li SM, Heide L.
  Title
CloR, a bifunctional non-heme iron oxygenase involved in clorobiocin biosynthesis.
  Journal
J Biol Chem 278:30661-8 (2003)
DOI:10.1074/jbc.M303190200
  Sequence
[ag:AAN65240]
LinkDB

KEGG   ENZYME: 1.13.11.83
Entry
EC 1.13.11.83               Enzyme                                 
Name
4-hydroxy-3-prenylphenylpyruvate oxygenase;
CloR
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
Sysname
3-(4-hydroxy-3-prenylphenyl)pyruvate:oxygen 1,2-oxidoreductase (4-hydroxy-3-prenylmandelate-forming)
Reaction(IUBMB)
3-(4-hydroxy-3-prenylphenyl)pyruvate + O2 = 4-hydroxy-3-prenylmandelate + CO2 [RN:R06758]
Reaction(KEGG)
R06758
Substrate
3-(4-hydroxy-3-prenylphenyl)pyruvate [CPD:C12456];
O2 [CPD:C00007]
Product
4-hydroxy-3-prenylmandelate [CPD:C12457];
CO2 [CPD:C00011]
Comment
Requires non-heme-iron(II). Isolated from the bacterium Streptomyces roseochromogenes DS 12976. A bifunctional enzyme involved in clorobiocin biosynthesis that also catalyses the activity of EC 1.13.12.23, 4-hydroxy-3-prenylbenzoate synthase.
History
EC 1.13.11.83 created 2017
Pathway
ec00401  Novobiocin biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K12708  4-hydroxy-3-prenylphenylpyruvate oxygenase / 4-hydroxy-3-prenylbenzoate synthase
Genes
NGV: CDO52_02235
Reference
1  [PMID:12777382]
  Authors
Pojer F, Kahlich R, Kammerer B, Li SM, Heide L.
  Title
CloR, a bifunctional non-heme iron oxygenase involved in clorobiocin biosynthesis.
  Journal
J Biol Chem 278:30661-8 (2003)
DOI:10.1074/jbc.M303190200
  Sequence
[ag:AAN65240]
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.83
IUBMB Enzyme Nomenclature: 1.13.11.83
ExPASy - ENZYME nomenclature database: 1.13.11.83
BRENDA, the Enzyme Database: 1.13.11.83
LinkDB

KEGG   REACTION: R06758
Entry
R06758                      Reaction                               
Name
3-(4-hydroxy-3-prenylphenyl)pyruvate:oxygen 1,2-oxidoreductase (4-hydroxy-3-prenylmandelate-forming)
Definition
3-Dimethylallyl-4-hydroxyphenylpyruvate + Oxygen <=> 3-Dimethylallyl-4-hydroxymandelic acid + CO2
Equation
C12456 + C00007 <=> C12457 + C00011
Reaction class
RC01471  C12456_C12457
Enzyme
1.13.11.83
Pathway
rn00401  Novobiocin biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.13  Acting on single donors with incorporation of molecular oxygen (oxygenases)
   1.13.11  With incorporation of two atoms of oxygen
    1.13.11.83
     R06758  3-Dimethylallyl-4-hydroxyphenylpyruvate + Oxygen <=> 3-Dimethylallyl-4-hydroxymandelic acid + CO2
Orthology
K12708  4-hydroxy-3-prenylphenylpyruvate oxygenase / 4-hydroxy-3-prenylbenzoate synthase [EC:1.13.11.83 1.13.12.23]
Other DBs
RHEA: 52911
LinkDB

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