KEGG ORTHOLOGY: K12711

ORTHOLOGY: K12711

Entry

K12711                      KO

Symbol

novM, cloM, couM

Name

L-demethylnoviosyl transferase [EC:2.4.1.302]

Pathway

map00401

Novobiocin biosynthesis

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Reaction

R06767

R06770

dTDP-4-O-demethyl-beta-L-noviose:novobiocic-acid 7-O-[4-O-demethyl-L-noviosyl]transferase

R06773

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00401 Novobiocin biosynthesis
    K12711  novM, cloM, couM; L-demethylnoviosyl transferase
Enzymes [BR:ko01000]
2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.302  L-demethylnoviosyl transferase
     K12711  novM, cloM, couM; L-demethylnoviosyl transferase

Other DBs

CAZy:

GT1

Genes

NSPU:

IFM12276_32170

SANL:

KZO11_29590

SGRF:

SGFS_016160

SCYE:

R2B67_24655 R2B67_24840

SPHW:

NFX46_24910(mtmGII)

SCAE:

IHE65_08945

SSPN:

LXH13_14010 LXH13_14045

STUD:

STRTU_005087

KBU:	Q4V64_41980

SPRF:

K4G22_02670

STPB:

QR97_38705

KCH:	O1G21_27160

NGV:	CDO52_02205

NCG:	KGD84_13355

APRT:

MUY14_02385

DVC:	Dvina_05705 Dvina_40140

DFU:	Dfulv_30410

DMAT:

Dmats_35010

DACT:

KZZ52_34315

AG:	AAF67506(novM) AAG29785(couM) AAN65229(cloM)

» show all

Reference

PMID:10770754

Authors

Steffensky M, Muhlenweg A, Wang ZX, Li SM, Heide L

Title

Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891.

Journal

Antimicrob Agents Chemother 44:1214-22 (2000)
DOI:10.1128/AAC.44.5.1214-1222.2000

Sequence

[ag:AAF67506]

Reference

PMID:12480894

Authors

Pojer F, Li SM, Heide L.

Title

Molecular cloning and sequence analysis of the clorobiocin biosynthetic gene cluster: new insights into the biosynthesis of aminocoumarin antibiotics.

Journal

Microbiology 148:3901-11 (2002)
DOI:10.1099/00221287-148-12-3901

Sequence

[ag:AAN65229]

Reference

PMID:15554710

Authors

Freel Meyers CL, Oberthur M, Heide L, Kahne D, Walsh CT

Title

Assembly of dimeric variants of coumermycins by tandem action of the four biosynthetic enzymes CouL, CouM, CouP, and NovN.

Journal

Biochemistry 43:15022-36 (2004)
DOI:10.1021/bi048457z

LinkDB

ENZYME: 2.4.1.302

Entry

EC 2.4.1.302                Enzyme

Name

L-demethylnoviosyl transferase;
novM (gene name);
dTDP-beta-L-noviose:novobiocic acid 7-O-noviosyltransferase;
L-noviosyl transferase

Class

Transferases;
Glycosyltransferases;
Hexosyltransferases

Sysname

dTDP-4-O-demethyl-beta-L-noviose:novobiocic acid 7-O-[4-O-demethyl-L-noviosyl]transferase

Reaction(IUBMB)

dTDP-4-O-demethyl-beta-L-noviose + novobiocic acid = dTDP + demethyldecarbamoyl novobiocin [RN:R06770]

Reaction(KEGG)

R06770

Substrate

dTDP-4-O-demethyl-beta-L-noviose [CPD:C12481];
novobiocic acid [CPD:C12474]

Product

dTDP [CPD:C00363];
demethyldecarbamoyl novobiocin

Comment

The enzyme is involved in the biosynthesis of the aminocoumarin antibiotic, novobiocin.

History

EC 2.4.1.302 created 2013, modified 2016

Pathway

ec00401

Novobiocin biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K12711

L-demethylnoviosyl transferase

Genes

NSPU:

IFM12276_32170

SANL:

KZO11_29590

SGRF:

SGFS_016160

SCYE:

R2B67_24655 R2B67_24840

SPHW:

NFX46_24910(mtmGII)

SCAE:

IHE65_08945

SSPN:

LXH13_14010 LXH13_14045

STUD:

STRTU_005087

KBU:	Q4V64_41980

SPRF:

K4G22_02670

STPB:

QR97_38705

KCH:	O1G21_27160

NGV:	CDO52_02205

NCG:	KGD84_13355

APRT:

MUY14_02385

DVC:	Dvina_05705 Dvina_40140

DFU:	Dfulv_30410

DMAT:

Dmats_35010

DACT:

KZZ52_34315

» show all

Reference

1 [PMID:12680772]

Authors

Freel Meyers CL, Oberthur M, Anderson JW, Kahne D, Walsh CT

Title

Initial characterization of novobiocic acid noviosyl transferase activity of NovM in biosynthesis of the antibiotic novobiocin.

Journal

Biochemistry 42:4179-89 (2003)
DOI:10.1021/bi0340088

Sequence

[ag:AAF67506]

Reference

2 [PMID:12633109]

Authors

Albermann C, Soriano A, Jiang J, Vollmer H, Biggins JB, Barton WA, Lesniak J, Nikolov DB, Thorson JS

Title

Substrate specificity of NovM: implications for novobiocin biosynthesis and glycorandomization.

Journal

Org Lett 5:933-6 (2003)
DOI:10.1021/ol0341086

Sequence

[ag:AAF67506]

Other DBs

ExplorEnz - The Enzyme Database:

2.4.1.302

IUBMB Enzyme Nomenclature:

2.4.1.302

ExPASy - ENZYME nomenclature database:

2.4.1.302

BRENDA, the Enzyme Database:

2.4.1.302

LinkDB

REACTION: R06770

Entry

R06770                      Reaction

Name

dTDP-4-O-demethyl-beta-L-noviose:novobiocic-acid 7-O-[4-O-demethyl-L-noviosyl]transferase

Definition

Novobiocic acid + dTDP-5-dimethyl-L-lyxose <=> Demethyldecarbamoylnovobiocin + dTDP

Equation

C12474 + C12481 <=> C12475 + C00363

Reaction class

RC00005

C00363_C12481

RC00171

C12474_C12475

Enzyme

2.4.1.302

Pathway

rn00401

Novobiocin biosynthesis

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

Brite

Enzymatic reactions [BR:br08201]
2. Transferase reactions
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.302
     R06770  Novobiocic acid + dTDP-5-dimethyl-L-lyxose <=> Demethyldecarbamoylnovobiocin + dTDP

Orthology

K12711

L-demethylnoviosyl transferase [EC:2.4.1.302]

Other DBs

RHEA:

36698

LinkDB

DBGET integrated database retrieval system