KEGG ORTHOLOGY: K12712

ORTHOLOGY: K12712

Entry

K12712                      KO

Symbol

novP, cloP, couP

Name

demethyldecarbamoylnovobiocin O-methyltransferase [EC:2.1.1.285]

Pathway

map00401

Novobiocin biosynthesis

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

Reaction

R06767

R06771

S-adenosyl-L-methionine:demethyldecarbamoylnovobiocin 4''-O-methyltransferase

R06774

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00401 Novobiocin biosynthesis
    K12712  novP, cloP, couP; demethyldecarbamoylnovobiocin O-methyltransferase
Enzymes [BR:ko01000]
2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.285  demethyldecarbamoylnovobiocin O-methyltransferase
     K12712  novP, cloP, couP; demethyldecarbamoylnovobiocin O-methyltransferase

Genes

PFAK:

KSS94_27025

SAOV:

G3H79_01915

SFP:	QUY26_02200

NGV:	CDO52_02225

ACTU:

Actkin_05240(mycF_2)

PMET:

G4Y79_24100

AMOB:

HG15A2_31330(novP)

AG:	AAF67509(novP) AAG29794(couP) AAN65238(cloP)

Reference

PMID:10770754

Authors

Steffensky M, Muhlenweg A, Wang ZX, Li SM, Heide L

Title

Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891.

Journal

Antimicrob Agents Chemother 44:1214-22 (2000)
DOI:10.1128/AAC.44.5.1214-1222.2000

Sequence

[ag:AAF67509]

Reference

PMID:12480894

Authors

Pojer F, Li SM, Heide L.

Title

Molecular cloning and sequence analysis of the clorobiocin biosynthetic gene cluster: new insights into the biosynthesis of aminocoumarin antibiotics.

Journal

Microbiology 148:3901-11 (2002)
DOI:10.1099/00221287-148-12-3901

Sequence

[ag:AAN65238]

Reference

PMID:15554710

Authors

Freel Meyers CL, Oberthur M, Heide L, Kahne D, Walsh CT

Title

Assembly of dimeric variants of coumermycins by tandem action of the four biosynthetic enzymes CouL, CouM, CouP, and NovN.

Journal

Biochemistry 43:15022-36 (2004)
DOI:10.1021/bi048457z

LinkDB

ENZYME: 2.1.1.285

Entry

EC 2.1.1.285                Enzyme

Name

demethyldecarbamoylnovobiocin O-methyltransferase;
NovP

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:demethyldecarbamoylnovobiocin 4''-O-methyltransferase

Reaction(IUBMB)

S-adenosyl-L-methionine + demethyldecarbamoylnovobiocin = S-adenosyl-L-homocysteine + decarbamoylnovobiocin [RN:R06771]

Reaction(KEGG)

R06771

Substrate

S-adenosyl-L-methionine [CPD:C00019];
demethyldecarbamoylnovobiocin [CPD:C12475]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
decarbamoylnovobiocin [CPD:C12476]

Comment

The enzyme is involved in the biosynthesis of the aminocoumarin antibiotic novobiocin.

History

EC 2.1.1.285 created 2013

Pathway

ec00401

Novobiocin biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K12712

demethyldecarbamoylnovobiocin O-methyltransferase

Genes

PFAK:

KSS94_27025

SAOV:

G3H79_01915

SFP:	QUY26_02200

NGV:	CDO52_02225

ACTU:

Actkin_05240(mycF_2)

PMET:

G4Y79_24100

AMOB:

HG15A2_31330(novP)

Reference

1 [PMID:14694473]

Authors

Freel Meyers CL, Oberthur M, Xu H, Heide L, Kahne D, Walsh CT

Title

Characterization of NovP and NovN: completion of novobiocin biosynthesis by sequential tailoring of the noviosyl ring.

Journal

Angew Chem Int Ed Engl 43:67-70 (2004)
DOI:10.1002/anie.200352626

Sequence

[ag:AAF67509]

Reference

2 [PMID:19857499]

Authors

Gomez Garcia I, Stevenson CE, Uson I, Freel Meyers CL, Walsh CT, Lawson DM

Title

The crystal structure of the novobiocin biosynthetic enzyme NovP: the first representative structure for the TylF O-methyltransferase superfamily.

Journal

J Mol Biol 395:390-407 (2010)
DOI:10.1016/j.jmb.2009.10.045

Sequence

[ag:AAF67509]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.285

IUBMB Enzyme Nomenclature:

2.1.1.285

ExPASy - ENZYME nomenclature database:

2.1.1.285

BRENDA, the Enzyme Database:

2.1.1.285

LinkDB

REACTION: R06771

Entry

R06771                      Reaction

Name

S-adenosyl-L-methionine:demethyldecarbamoylnovobiocin 4''-O-methyltransferase

Definition

Demethyldecarbamoylnovobiocin + S-Adenosyl-L-methionine <=> Decarbamoylnovobiocin + S-Adenosyl-L-homocysteine

Equation

C12475 + C00019 <=> C12476 + C00021

Reaction class

RC00003

C00019_C00021

RC00466

C12475_C12476

Enzyme

2.1.1.285

Pathway

rn00401

Novobiocin biosynthesis

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

Brite

Enzymatic reactions [BR:br08201]
2. Transferase reactions
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.285
     R06771  Demethyldecarbamoylnovobiocin + S-Adenosyl-L-methionine <=> Decarbamoylnovobiocin + S-Adenosyl-L-homocysteine

Orthology

K12712

demethyldecarbamoylnovobiocin O-methyltransferase [EC:2.1.1.285]

Other DBs

RHEA:

36658

LinkDB

DBGET integrated database retrieval system