KEGG   ORTHOLOGY: K12713
Entry
K12713                      KO                                     
Symbol
novN
Name
decarbamoylnovobiocin carbamoyltransferase [EC:2.1.3.12]
Pathway
map00401  Novobiocin biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Reaction
R06772  carbamoyl phosphate:decarbamoylnovobiocin 3''-O-carbamoyltransferase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00401 Novobiocin biosynthesis
    K12713  novN; decarbamoylnovobiocin carbamoyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.3  Carboxy- and carbamoyltransferases
    2.1.3.12  decarbamoylnovobiocin carbamoyltransferase
     K12713  novN; decarbamoylnovobiocin carbamoyltransferase
Genes
SLUO: PS467_34415
STRH: GXP74_22395
NGV: CDO52_02210
ACTU: Actkin_02921
SNEP: Enr13x_07980(novN_1)
AG: AAF67507(novN)
Reference
PMID:10770754
  Authors
Steffensky M, Muhlenweg A, Wang ZX, Li SM, Heide L
  Title
Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891.
  Journal
Antimicrob Agents Chemother 44:1214-22 (2000)
DOI:10.1128/AAC.44.5.1214-1222.2000
  Sequence
[ag:AAF67507]
LinkDB

KEGG   ENZYME: 2.1.3.12
Entry
EC 2.1.3.12                 Enzyme                                 
Name
decarbamoylnovobiocin carbamoyltransferase;
novN (gene name)
Class
Transferases;
Transferring one-carbon groups;
Carboxy- and carbamoyltransferases
Sysname
carbamoyl phosphate:decarbamoylnovobiocin 3''-O-carbamoyltransferase
Reaction(IUBMB)
carbamoyl phosphate + decarbamoylnovobiocin = phosphate + novobiocin [RN:R06772]
Reaction(KEGG)
R06772
Substrate
carbamoyl phosphate [CPD:C00169];
decarbamoylnovobiocin [CPD:C12476]
Product
phosphate [CPD:C00009];
novobiocin [CPD:C05080]
Comment
The enzyme catalyses the last step in the biosynthesis of the aminocoumarin antibiotic novobiocin. The reaction is activated by ATP [1].
History
EC 2.1.3.12 created 2013
Pathway
ec00401  Novobiocin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12713  decarbamoylnovobiocin carbamoyltransferase
Genes
SLUO: PS467_34415
STRH: GXP74_22395
NGV: CDO52_02210
ACTU: Actkin_02921
SNEP: Enr13x_07980(novN_1)
Reference
1  [PMID:14694473]
  Authors
Freel Meyers CL, Oberthur M, Xu H, Heide L, Kahne D, Walsh CT
  Title
Characterization of NovP and NovN: completion of novobiocin biosynthesis by sequential tailoring of the noviosyl ring.
  Journal
Angew Chem Int Ed Engl 43:67-70 (2004)
DOI:10.1002/anie.200352626
  Sequence
[ag:AAF67507]
Reference
2  [PMID:18997325]
  Authors
Gomez Garcia I, Freel Meyers CL, Walsh CT, Lawson DM
  Title
Crystallization and preliminary X-ray analysis of the O-carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides.
  Journal
Acta Crystallogr Sect F Struct Biol Cryst Commun 64:1000-2 (2008)
DOI:10.1107/S1744309108030145
  Sequence
[ag:AAF67507]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.3.12
IUBMB Enzyme Nomenclature: 2.1.3.12
ExPASy - ENZYME nomenclature database: 2.1.3.12
BRENDA, the Enzyme Database: 2.1.3.12
LinkDB

KEGG   REACTION: R06772
Entry
R06772                      Reaction                               
Name
carbamoyl phosphate:decarbamoylnovobiocin 3''-O-carbamoyltransferase
Definition
Decarbamoylnovobiocin + Carbamoyl phosphate <=> Novobiocin + Orthophosphate
Equation
C12476 + C00169 <=> C05080 + C00009
Reaction class
RC00055  C05080_C12476
Enzyme
2.1.3.12
Pathway
rn00401  Novobiocin biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.1  Transferring one-carbon groups
   2.1.3  Carboxy- and carbamoyltransferases
    2.1.3.12
     R06772  Decarbamoylnovobiocin + Carbamoyl phosphate <=> Novobiocin + Orthophosphate
Orthology
K12713  decarbamoylnovobiocin carbamoyltransferase [EC:2.1.3.12]
Other DBs
RHEA: 36662
LinkDB

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