KEGG   ORTHOLOGY: K13070
Entry
K13070            Tight     KO                                     
Symbol
MAS
Name
momilactone-A synthase [EC:1.1.1.295]
Pathway
map00904  Diterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R08916  3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide:NAD+ oxidoreductase
R08917  3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide:NADP+ oxidoreductase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K13070  MAS; momilactone-A synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.295  momilactone-A synthase
     K13070  MAS; momilactone-A synthase
Genes
OSA: 4335093(OsJ_13988)
OGL: 127769885
Reference
PMID:17872948
  Authors
Shimura K, Okada A, Okada K, Jikumaru Y, Ko KW, Toyomasu T, Sassa T, Hasegawa M, Kodama O, Shibuya N, Koga J, Nojiri H, Yamane H
  Title
Identification of a biosynthetic gene cluster in rice for momilactones.
  Journal
J Biol Chem 282:34013-8 (2007)
DOI:10.1074/jbc.M703344200
  Sequence
[osa:4335093]
Reference
PMID:12005050
  Authors
Atawong A, Hasegawa M, Kodama O
  Title
Biosynthesis of rice phytoalexin: enzymatic conversion of 3beta-hydroxy-9beta-pimara-7,15-dien-19,6beta-olide to momilactone A.
  Journal
Biosci Biotechnol Biochem 66:566-70 (2002)
DOI:10.1271/bbb.66.566
  Sequence
[osa:4335093]
LinkDB

KEGG   ENZYME: 1.1.1.295
Entry
EC 1.1.1.295                Enzyme                                 
Name
momilactone-A synthase;
momilactone A synthase;
OsMAS
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide:NAD(P)+ oxidoreductase
Reaction(IUBMB)
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NAD(P)+ = momilactone A + NAD(P)H + H+ [RN:R08916 R08917]
Reaction(KEGG)
R08916 R08917
Substrate
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide [CPD:C18016];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
momilactone A [CPD:C18015];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The rice phytoalexin momilactone A is a diterpenoid secondary metabolite that is involved in the defense mechanism of the plant. Momilactone A is produced in response to attack by a pathogen through the perception of elicitor signal molecules such as chitin oligosaccharide, or after exposure to UV irradiation. The enzyme, which catalyses the last step in the biosynthesis of momilactone A, can use both NAD+ and NADP+ but activity is higher with NAD+ [1].
History
EC 1.1.1.295 created 2008
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13070  momilactone-A synthase
Genes
OSA: 4335093(OsJ_13988)
OGL: 127769885
Reference
1  [PMID:12005050]
  Authors
Atawong A, Hasegawa M, Kodama O
  Title
Biosynthesis of rice phytoalexin: enzymatic conversion of 3beta-hydroxy-9beta-pimara-7,15-dien-19,6beta-olide to momilactone A.
  Journal
Biosci Biotechnol Biochem 66:566-70 (2002)
DOI:10.1271/bbb.66.566
  Sequence
[osa:4335093]
Reference
2  [PMID:17872948]
  Authors
Shimura K, Okada A, Okada K, Jikumaru Y, Ko KW, Toyomasu T, Sassa T, Hasegawa M, Kodama O, Shibuya N, Koga J, Nojiri H, Yamane H
  Title
Identification of a biosynthetic gene cluster in rice for momilactones.
  Journal
J Biol Chem 282:34013-8 (2007)
DOI:10.1074/jbc.M703344200
  Sequence
[osa:4335093]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.295
IUBMB Enzyme Nomenclature: 1.1.1.295
ExPASy - ENZYME nomenclature database: 1.1.1.295
BRENDA, the Enzyme Database: 1.1.1.295
LinkDB

KEGG   REACTION: R08916
Entry
R08916                      Reaction                               
Name
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide:NAD+ oxidoreductase
Definition
3beta-Hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NAD+ <=> Momilactone A + NADH + H+
Equation
C18016 + C00003 <=> C18015 + C00004 + C00080
Comment
NADP+ (less effective, see R08917)
Reaction class
RC00001  C00003_C00004
RC00127  C18015_C18016
Enzyme
1.1.1.295
Pathway
rn00904  Diterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.295
     R08916  3beta-Hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NAD+ <=> Momilactone A + NADH + H+
Orthology
K13070  momilactone-A synthase [EC:1.1.1.295]
Other DBs
RHEA: 25366
LinkDB

KEGG   REACTION: R08917
Entry
R08917                      Reaction                               
Name
3beta-hydroxy-9beta-pimara-7,15-diene-19,6beta-olide:NADP+ oxidoreductase
Definition
3beta-Hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NADP+ <=> Momilactone A + NADPH + H+
Equation
C18016 + C00006 <=> C18015 + C00005 + C00080
Comment
NAD+ (more effective, see R08916)
Reaction class
RC00001  C00005_C00006
RC00127  C18015_C18016
Enzyme
1.1.1.295
Pathway
rn00904  Diterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.295
     R08917  3beta-Hydroxy-9beta-pimara-7,15-diene-19,6beta-olide + NADP+ <=> Momilactone A + NADPH + H+
Orthology
K13070  momilactone-A synthase [EC:1.1.1.295]
Other DBs
RHEA: 25370
LinkDB

DBGET integrated database retrieval system