KEGG   ORTHOLOGY: K13222
Entry
K13222                      KO                                     

Name
BX1, IGL
Definition
indole-3-glycerol-phosphate lyase [EC:4.1.2.8]
Pathway
ko00402  Benzoxazinoid biosynthesis
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00402 Benzoxazinoid biosynthesis
    K13222  BX1, IGL; indole-3-glycerol-phosphate lyase
Enzymes [BR:ko01000]
 4. Lyases
  4.1  Carbon-carbon lyases
   4.1.2  Aldehyde-lyases
    4.1.2.8  indole-3-glycerol-phosphate lyase
     K13222  BX1, IGL; indole-3-glycerol-phosphate lyase
Other DBs
RN: R02340
GO: 0033984
Genes
OSA: 4334427 9268807 9270552
DOSA: Os03t0797400-01(Os03g0797400) Os03t0797500-01(Os03g0797500)
OBR: 102712344 102719945
BDI: 100834829 100836811 100843076
ATS: 109757378 109757925 109758460 109758461 109758465 109758467
SBI: 8061167 8081255
ZMA: 100273163(csu868(trp)) 103644182 542117(bx1)
SITA: 101766758 101767173
Reference
PMID:9371848
  Authors
Melanson D, Chilton MD, Masters-Moore D, Chilton WS
  Title
A deletion in an indole synthase gene is responsible for the DIMBOA-deficient phenotype of bxbx maize.
  Journal
Proc Natl Acad Sci U S A 94:13345-50 (1997)
DOI:10.1073/pnas.94.24.13345
Reference
PMID:9235894 (BX1)
  Authors
Frey M, Chomet P, Glawischnig E, Stettner C, Grun S, Winklmair A, Eisenreich W, Bacher A, Meeley RB, Briggs SP, Simcox K, Gierl A.
  Title
Analysis of a chemical plant defense mechanism in grasses.
  Journal
Science 277:696-9 (1997)
DOI:10.1126/science.277.5326.696
  Sequence
[zma:542117]
LinkDB

KEGG   ENZYME: 4.1.2.8
Entry
EC 4.1.2.8                  Enzyme                                 

Name
indole-3-glycerol-phosphate lyase;
tryptophan synthase alpha;
TSA;
indoleglycerolphosphate aldolase;
indole glycerol phosphate hydrolase;
indole synthase;
indole-3-glycerolphosphate D-glyceraldehyde-3-phosphate-lyase;
indole-3-glycerol phosphate lyase;
IGL;
BX1;
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate D-glyceraldehyde-3-phosphate-lyase
Class
Lyases;
Carbon-carbon lyases;
Aldehyde-lyases
Sysname
(1S,2R)-1-C-(indol-3-yl)glycerol-3-phosphate D-glyceraldehyde-3-phosphate-lyase (indole-forming)
Reaction(IUBMB)
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate [RN:R02340]
Reaction(KEGG)
R02340
Substrate
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate [CPD:C03506]
Product
indole [CPD:C00463];
D-glyceraldehyde 3-phosphate [CPD:C00118]
Comment
Forms part of the defence mechanism against insects and microbial pathogens in the grass family, Gramineae, where it catalyses the first committed step in the formation of the cyclic hydroxamic acids 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one (DIBOA) and 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA) [1]. This enzyme resembles the alpha-subunit of EC 4.2.1.20, tryptophan synthase [3], for which, (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate is also a substrate, but, unlike tryptophan synthase, its activity is independent of the beta-subunit and free indole is released [2].
History
EC 4.1.2.8 created 1961, deleted 1972, reinstated 2006
Pathway
ec00402  Benzoxazinoid biosynthesis
ec01100  Metabolic pathways
Orthology
K13222  indole-3-glycerol-phosphate lyase
Genes
OSA: 4334427 9268807 9270552
DOSA: Os03t0797400-01(Os03g0797400) Os03t0797500-01(Os03g0797500)
OBR: 102712344 102719945
BDI: 100834829 100836811 100843076
ATS: 109757378 109757925 109758460 109758461 109758465 109758467
SBI: 8061167 8081255
ZMA: 100273163(csu868(trp)) 103644182 542117(bx1)
SITA: 101766758 101767173
Reference
1  [PMID:13376586]
  Authors
YANOFSKY C.
  Title
The enzymatic conversion of anthranilic acid to indole.
  Journal
J Biol Chem 223:171-84 (1956)
Reference
2  [PMID:9235894]
  Authors
Frey M, Chomet P, Glawischnig E, Stettner C, Grun S, Winklmair A, Eisenreich W, Bacher A, Meeley RB, Briggs SP, Simcox K, Gierl A.
  Title
Analysis of a chemical plant defense mechanism in grasses.
  Journal
Science 277:696-9 (1997)
DOI:10.1126/science.277.5326.696
  Sequence
[zma:542117]
Reference
3  [PMID:11106389]
  Authors
Frey M, Stettner C, Pare PW, Schmelz EA, Tumlinson JH, Gierl A.
  Title
An herbivore elicitor activates the gene for indole emission in maize.
  Journal
Proc Natl Acad Sci U S A 97:14801-6 (2000)
DOI:10.1073/pnas.260499897
Reference
4  [PMID:9371848]
  Authors
Melanson D, Chilton MD, Masters-Moore D, Chilton WS
  Title
A deletion in an indole synthase gene is responsible for the DIMBOA-deficient phenotype of bxbx maize.
  Journal
Proc Natl Acad Sci U S A 94:13345-50 (1997)
DOI:10.1073/pnas.94.24.13345
Other DBs
ExplorEnz - The Enzyme Database: 4.1.2.8
IUBMB Enzyme Nomenclature: 4.1.2.8
ExPASy - ENZYME nomenclature database: 4.1.2.8
BRENDA, the Enzyme Database: 4.1.2.8
CAS: 9014-52-2
LinkDB

KEGG   REACTION: R02340
Entry
R02340                      Reaction                               

Name
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate D-glyceraldehyde-3-phosphate-lyase
Definition
Indoleglycerol phosphate <=> Indole + D-Glyceraldehyde 3-phosphate
Equation
Comment
a part of multi-step reaction (ec 4.2.1.20, see R02722, R02340+R00674)
Reaction class
RC00700  C00118_C03506
RC00701  C00463_C03506
Enzyme
4.1.2.8         4.2.1.20
Pathway
rn00400  Phenylalanine, tyrosine and tryptophan biosynthesis
rn00402  Benzoxazinoid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K01695  tryptophan synthase alpha chain [EC:4.2.1.20]
K01696  tryptophan synthase beta chain [EC:4.2.1.20]
K06001  tryptophan synthase beta chain [EC:4.2.1.20]
K13222  indole-3-glycerol-phosphate lyase [EC:4.1.2.8]
Other DBs
RHEA: 14084
LinkDB

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