KEGG   ORTHOLOGY: K13223
Entry
K13223                      KO                                     

Name
BX2, CYP71C4, CYP71C9
Definition
indole-2-monooxygenase [EC:1.14.14.153]
Pathway
ko00402  Benzoxazinoid biosynthesis
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00402 Benzoxazinoid biosynthesis
    K13223  BX2, CYP71C4, CYP71C9; indole-2-monooxygenase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K13223  BX2, CYP71C4, CYP71C9; indole-2-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.153  indole-2-monooxygenase
     K13223  BX2, CYP71C4, CYP71C9; indole-2-monooxygenase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP71 family
   K13223  BX2, CYP71C4, CYP71C9; indole-2-monooxygenase
Other DBs
RN: R07403
Genes
ATS: 109757929
ZMA: 100192631(bx2)
AG: BAB87820(CYP71C9v1) BAB87821(CYP71C9v2) CAA72196(BX2)
Reference
PMID:9235894 (CYP71C4)
  Authors
Frey M, Chomet P, Glawischnig E, Stettner C, Grun S, Winklmair A, Eisenreich W, Bacher A, Meeley RB, Briggs SP, Simcox K, Gierl A.
  Title
Analysis of a chemical plant defense mechanism in grasses.
  Journal
Science 277:696-9 (1997)
DOI:10.1126/science.277.5326.696
  Sequence
Reference
PMID:7823905
  Authors
Frey M, Kliem R, Saedler H, Gierl A
  Title
Expression of a cytochrome P450 gene family in maize.
  Journal
Mol Gen Genet 246:100-9 (1995)
DOI:10.1007/BF00290138
Reference
PMID:11976964 (CYP71C9)
  Authors
Nomura T, Ishihara A, Imaishi H, Endo TR, Ohkawa H, Iwamura H
  Title
Molecular characterization and chromosomal localization of cytochrome P450 genes involved in the biosynthesis of cyclic hydroxamic acids in hexaploid wheat.
  Journal
Mol Genet Genomics 267:210-7 (2002)
DOI:10.1007/s00438-002-0653-x
  Sequence
LinkDB

KEGG   ENZYME: 1.14.14.153
Entry
EC 1.14.14.153              Enzyme                                 

Name
indole-2-monooxygenase;
BX2 (gene name);
CYP71C4 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
indole,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
indole + [reduced NADPH---hemoprotein reductase] + O2 = indolin-2-one + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R07403]
Reaction(KEGG)
R07403
Substrate
indole [CPD:C00463];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
indolin-2-one [CPD:C12312];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme is involved in the biosynthesis of protective and allelophatic benzoxazinoids in some plants, most commonly from the family of Poaceae (grasses).
History
EC 1.14.14.153 created 2012 as EC 1.14.13.137, transferred 2018 to EC 1.14.14.153
Pathway
ec00402  Benzoxazinoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13223  indole-2-monooxygenase
Genes
ATS: 109757929
ZMA: 100192631(bx2)
Reference
1  [PMID:9235894]
  Authors
Frey M, Chomet P, Glawischnig E, Stettner C, Grun S, Winklmair A, Eisenreich W, Bacher A, Meeley RB, Briggs SP, Simcox K, Gierl A.
  Title
Analysis of a chemical plant defense mechanism in grasses.
  Journal
Science 277:696-9 (1997)
DOI:10.1126/science.277.5326.696
  Sequence
Reference
2  [PMID:10385992]
  Authors
Glawischnig E, Grun S, Frey M, Gierl A
  Title
Cytochrome P450 monooxygenases of DIBOA biosynthesis: specificity and conservation among grasses.
  Journal
Phytochemistry 50:925-30 (1999)
DOI:10.1016/S0031-9422(98)00318-5
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.153
IUBMB Enzyme Nomenclature: 1.14.14.153
ExPASy - ENZYME nomenclature database: 1.14.14.153
BRENDA, the Enzyme Database: 1.14.14.153
LinkDB

KEGG   REACTION: R07403
Entry
R07403                      Reaction                               

Name
indole,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2-hydroxylating)
Definition
Indole + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> Indolin-2-one + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
Reaction class
RC01834  C00463_C12312
Enzyme
Pathway
rn00402  Benzoxazinoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Orthology
K13223  indole-2-monooxygenase [EC:1.14.14.153]
Other DBs
RHEA: 31902
LinkDB

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