KEGG   ORTHOLOGY: K13227
Entry
K13227                      KO                                     

Name
BX8_9
Definition
UDP-glucosyltransferase BX8/BX9 [EC:2.4.1.202]
Pathway
ko00402  Benzoxazinoid biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00402 Benzoxazinoid biosynthesis
    K13227  BX8_9; UDP-glucosyltransferase BX8/BX9
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.202  2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase
     K13227  BX8_9; UDP-glucosyltransferase BX8/BX9
Other DBs
RN: R04579 R07426
GO: 0047254
CAZy: GT1
Genes
OSA: 4342809 4350429
DOSA: Os11t0444000-01(Os11g0444000)
OBR: 102699351 102716206
BDI: 100830893
ATS: 109736907 109754422
SBI: 110430107 8069138
ZMA: 100274317(bx9) 100277344(bx8) 103632152
SITA: 101752637 101753321 101778310 101780325
Reference
  Authors
Bailey BA, Larson RL
  Title
Hydroxamic Acid glucosyltransferases from maize seedlings.
  Journal
Plant Physiol 90:1071-6 (1989)
DOI:10.1104/pp.90.3.1071
  Sequence
[zma:100274317]
Reference
  Authors
von Rad U, Huttl R, Lottspeich F, Gierl A, Frey M.
  Title
Two glucosyltransferases are involved in detoxification of benzoxazinoids in maize.
  Journal
Plant J 28:633-42 (2001)
DOI:10.1046/j.1365-313x.2001.01161.x
  Sequence
LinkDB

KEGG   ENZYME: 2.4.1.202
Entry
EC 2.4.1.202                Enzyme                                 

Name
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase;
uridine diphosphoglucose-2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-glucosyltransferase;
BX8;
BX9;
benzoxazinoid glucosyltransferase;
DIMBOA glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-alpha-D-glucose:2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-beta-D-glucosyltransferase
Reaction(IUBMB)
(1) UDP-alpha-D-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one = UDP + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside [RN:R04579];
(2) UDP-alpha-D-glucose + 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one = UDP + (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside [RN:R07426]
Reaction(KEGG)
R04579 R07426
Substrate
UDP-alpha-D-glucose [CPD:C00029];
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one [CPD:C04720];
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one [CPD:C15770]
Product
UDP [CPD:C00015];
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside [CPD:C04831];
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside [CPD:C15772]
Comment
The enzyme is involved in the detoxification of the benzoxazinoids DIBOA (2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one) and DIMBOA (2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one) which are stored as the respective non-toxic glucosides in the vacuoles in some plants, most commonly from the family of Poaceae (grasses). Benzoxazinoids are known to exhibit antimicrobial, antifeedant, and antiinsecticidal effects and are involved in the interaction of plants with other plants, insects, or microorganisms.
History
EC 2.4.1.202 created 1992, modified 2012
Pathway
ec00402  Benzoxazinoid biosynthesis
Orthology
K13227  UDP-glucosyltransferase BX8/BX9
Genes
OSA: 4342809 4350429
DOSA: Os11t0444000-01(Os11g0444000)
OBR: 102699351 102716206
BDI: 100830893
ATS: 109736907 109754422
SBI: 110430107 8069138
ZMA: 100274317(bx9) 100277344(bx8) 103632152
SITA: 101752637 101753321 101778310 101780325
Reference
1  [PMID:16666853]
  Authors
Bailey BA, Larson RL
  Title
Hydroxamic Acid glucosyltransferases from maize seedlings.
  Journal
Plant Physiol 90:1071-6 (1989)
DOI:10.1104/pp.90.3.1071
  Sequence
[zma:100274317]
Reference
2  [PMID:11851909]
  Authors
von Rad U, Huttl R, Lottspeich F, Gierl A, Frey M.
  Title
Two glucosyltransferases are involved in detoxification of benzoxazinoids in maize.
  Journal
Plant J 28:633-42 (2001)
DOI:10.1046/j.1365-313x.2001.01161.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.202
IUBMB Enzyme Nomenclature: 2.4.1.202
ExPASy - ENZYME nomenclature database: 2.4.1.202
BRENDA, the Enzyme Database: 2.4.1.202
CAS: 122544-56-3
LinkDB

KEGG   REACTION: R07426
Entry
R07426                      Reaction                               

Name
UDP-glucose:DIBOA 2-D-glucosyltransferase
Definition
UDP-glucose + DIBOA <=> UDP + DIBOA-glucoside
Equation
Comment
UDP-glucosyltransferase, bx8, bx9
Reaction class
RC00005  C00015_C00029
RC00049  C15770_C15772
Enzyme
Pathway
rn00402  Benzoxazinoid biosynthesis
Orthology
K13227  UDP-glucosyltransferase BX8/BX9 [EC:2.4.1.202]
Other DBs
RHEA: 33958
LinkDB

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