KEGG ORTHOLOGY: K13307

ORTHOLOGY: K13307

Entry

K13307                      KO

Symbol

tylMI

Name

dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase [EC:2.1.1.235]

Pathway

map00523

Polyketide sugar unit biosynthesis

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

map01250

Biosynthesis of nucleotide sugars

Module

M00796

dTDP-D-mycaminose biosynthesis

Reaction

R06439

S-adenosyl-L-methionine:dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose 3-N,N-dimethyltransferase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00523 Polyketide sugar unit biosynthesis
    K13307  tylMI; dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase
Enzymes [BR:ko01000]
2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.235  dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase
     K13307  tylMI; dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase

Genes

IDC:	LRM40_02210

PPAK:

JY651_27515

SAMB:

SAM23877_5630(srm7)

SGJ:	IAG43_33095

SMAO:

SAKB:

SCAE:

NAEI:

MAU:	Micau_3984

MIL:

ML5_4437

ARAM:

KAR29_01360

AG:	CAA57473(tylM1)

» show all

Reference

PMID:9031628

Authors

Gandecha AR, Large SL, Cundliffe E

Title

Analysis of four tylosin biosynthetic genes from the tylLM region of the Streptomyces fradiae genome.

Journal

Gene 184:197-203 (1997)
DOI:10.1016/S0378-1119(96)00595-1

Sequence

[ag:CAA57473]

LinkDB

ENZYME: 2.1.1.235

Entry

EC 2.1.1.235                Enzyme

Name

dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase;
TylM1

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose 3-N,N-dimethyltransferase

Reaction(IUBMB)

2 S-adenosyl-L-methionine + dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose = 2 S-adenosyl-L-homocysteine + dTDP-3-dimethylamino-3,6-dideoxy-alpha-D-glucopyranose [RN:R06439]

Reaction(KEGG)

R06439

Substrate

S-adenosyl-L-methionine [CPD:C00019];
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose

Product

S-adenosyl-L-homocysteine [CPD:C00021];
dTDP-3-dimethylamino-3,6-dideoxy-alpha-D-glucopyranose

Comment

The enzyme is involved in the biosynthesis of mycaminose, an essential structural component of the macrolide antibiotic tylosin, which is produced by the bacterium Streptomyces fradiae.

History

EC 2.1.1.235 created 2011

Pathway

ec00523

Polyketide sugar unit biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K13307

dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase

Genes

IDC:	LRM40_02210

PPAK:

JY651_27515

SAMB:

SAM23877_5630(srm7)

SGJ:	IAG43_33095

SMAO:

CAG99_12105

SAKB:

K1J60_45145

SCAE:

IHE65_08835

NAEI:

GCM126_00550

MAU:	Micau_3984

MIL:

ML5_4437

ARAM:

KAR29_01360

» show all

Reference

1 [PMID:12119032]

Authors

Chen H, Yamase H, Murakami K, Chang CW, Zhao L, Zhao Z, Liu HW

Title

Expression, purification, and characterization of two N,N-dimethyltransferases, tylM1 and desVI, involved in the biosynthesis of mycaminose and desosamine.

Journal

Biochemistry 41:9165-83 (2002)
DOI:10.1021/bi020245j

Sequence

[ag:CAA57473]

Reference

2 [PMID:21142177]

Authors

Carney AE, Holden HM

Title

Molecular architecture of TylM1 from Streptomyces fradiae: an N,N-dimethyltransferase involved in the production of dTDP-D-mycaminose.

Journal

Biochemistry 50:780-7 (2011)
DOI:10.1021/bi101733y

Sequence

[ag:CAA57473]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.235

IUBMB Enzyme Nomenclature:

2.1.1.235

ExPASy - ENZYME nomenclature database:

2.1.1.235

BRENDA, the Enzyme Database:

2.1.1.235

LinkDB

REACTION: R06439

Entry

R06439                      Reaction

Name

S-adenosyl-L-methionine:dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose 3-N,N-dimethyltransferase

Definition

dTDP-3-amino-3,6-dideoxy-D-glucose + 2 S-Adenosyl-L-methionine <=> dTDP-D-mycaminose + 2 S-Adenosyl-L-homocysteine + 2 H+

Equation

C11925 + 2 C00019 <=> C11933 + 2 C00021 + 2 C00080

Comment

N,N-dimethyltransferase,TylM1

Reaction class

RC00003

C00019_C00021

RC01515

C11925_C11933

Enzyme

2.1.1.235

Pathway

rn00523

Polyketide sugar unit biosynthesis

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

rn01250

Biosynthesis of nucleotide sugars

Module

M00796

dTDP-D-mycaminose biosynthesis

Brite

Enzymatic reactions [BR:br08201]
2. Transferase reactions
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.235
     R06439  dTDP-3-amino-3,6-dideoxy-D-glucose + 2 S-Adenosyl-L-methionine <=> dTDP-D-mycaminose + 2 S-Adenosyl-L-homocysteine + 2 H+

Orthology

K13307

dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase [EC:2.1.1.235]

Other DBs

RHEA:

31674

LinkDB

DBGET integrated database retrieval system