KEGG   ORTHOLOGY: K13312
Entry
K13312                      KO                                     
Symbol
tylJ, gerF
Name
dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase [EC:5.1.3.27]
Pathway
map00523  Polyketide sugar unit biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
map01250  Biosynthesis of nucleotide sugars
Module
M00794  dTDP-6-deoxy-D-allose biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00523 Polyketide sugar unit biosynthesis
    K13312  tylJ, gerF; dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
Enzymes [BR:ko01000]
 5. Isomerases
  5.1  Racemases and epimerases
   5.1.3  Acting on carbohydrates and derivatives
    5.1.3.27  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
     K13312  tylJ, gerF; dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
Other DBs
RN: R06437
Genes
STRM: M444_29900
AG: AAD12169(tylJ) ABB52524(gerF)
Reference
PMID:10220165 (tylJ)
  Authors
Fouces R, Mellado E, Diez B, Barredo JL
  Title
The tylosin biosynthetic cluster from Streptomyces fradiae: genetic organization of the left region.
  Journal
Microbiology 145 ( Pt 4):855-68 (1999)
DOI:10.1099/13500872-145-4-855
  Sequence
[ag:AAD12169]
Reference
PMID:17053005 (gerF)
  Authors
Thuy TT, Liou K, Oh TJ, Kim DH, Nam DH, Yoo JC, Sohng JK
  Title
Biosynthesis of dTDP-6-deoxy-beta-D-allose, biochemical characterization of dTDP-4-keto-6-deoxyglucose reductase (GerKI) from Streptomyces sp. KCTC 0041BP.
  Journal
Glycobiology 17:119-26 (2007)
DOI:10.1093/glycob/cwl060
  Sequence
[ag:ABB52524]
LinkDB

KEGG   ORTHOLOGY: K19855
Entry
K19855                      KO                                     
Symbol
novW
Name
dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase [EC:5.1.3.27]
Pathway
map00523  Polyketide sugar unit biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
map01250  Biosynthesis of nucleotide sugars
Module
M00795  dTDP-beta-L-noviose biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00523 Polyketide sugar unit biosynthesis
    K19855  novW; dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
Enzymes [BR:ko01000]
 5. Isomerases
  5.1  Racemases and epimerases
   5.1.3  Acting on carbohydrates and derivatives
    5.1.3.27  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
     K19855  novW; dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
Other DBs
RN: R06437
Genes
MCHN: HCR76_04910
NGV: CDO52_02260
AG: AAF67516(novW) AAG29805(couW) AAN65245(cloW) BAC57040(mydH)
Reference
PMID:10770754
  Authors
Steffensky M, Muhlenweg A, Wang ZX, Li SM, Heide L
  Title
Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891.
  Journal
Antimicrob Agents Chemother 44:1214-22 (2000)
DOI:10.1128/AAC.44.5.1214-1222.2000
  Sequence
[ag:AAF67516]
Reference
PMID:16514445
  Authors
Tello M, Jakimowicz P, Errey JC, Freel Meyers CL, Walsh CT, Buttner MJ, Lawson DM, Field RA
  Title
Characterisation of Streptomyces spheroides NovW and revision of its functional assignment to a dTDP-6-deoxy-D-xylo-4-hexulose 3-epimerase.
  Journal
Chem Commun (Camb) 1079-81 (2006)
DOI:10.1039/b515763c
LinkDB

KEGG   ENZYME: 5.1.3.27
Entry
EC 5.1.3.27                 Enzyme                                 
Name
dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase;
dTDP-deoxyglucose 3-epimerase;
dTDP-4-keto-6-deoxy-D-glucose 3-epimerase;
dTDP-4-keto-6-deoxyglucose 3-epimerase;
gerF (gene name);
tylJ (gene name);
chmJ (gene name);
mydH (gene name)
Class
Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives
Sysname
dTDP-4-dehydro-6-deoxy-alpha-D-glucose 3-epimerase
Reaction(IUBMB)
dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-gulose [RN:R06437]
Reaction(KEGG)
R06437
Substrate
dTDP-4-dehydro-6-deoxy-alpha-D-glucose [CPD:C11907]
Product
dTDP-4-dehydro-6-deoxy-alpha-D-gulose [CPD:C11926]
Comment
The enzyme is involved in the biosynthetic pathway of dTDP-6-deoxy-alpha-D-allose, which is converted to mycinose after attachment to the aglycones of several macrolide antibiotics, including tylosin, chalcomycin, dihydrochalcomycin, and mycinamicin II.
History
EC 5.1.3.27 created 2013
Pathway
ec00523  Polyketide sugar unit biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13312  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
K19855  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
Genes
STRM: M444_29900
MCHN: HCR76_04910
NGV: CDO52_02260
Reference
1  [PMID:15049360]
  Authors
Sohng JK, Kim HJ, Nam DH, Lim DO, Han JM, Lee HJ, Yoo JC
  Title
Cloning, expression, and biological function of a dTDP-deoxyglucose epimerase (gerF) gene from Streptomyces sp. GERI-155.
  Journal
Biotechnol Lett 26:185-91 (2004)
DOI:10.1023/b:bile.0000013709.80691.97
  Sequence
[ag:ABB52524]
Reference
2  [PMID:17053005]
  Authors
Thuy TT, Liou K, Oh TJ, Kim DH, Nam DH, Yoo JC, Sohng JK
  Title
Biosynthesis of dTDP-6-deoxy-beta-D-allose, biochemical characterization of dTDP-4-keto-6-deoxyglucose reductase (GerKI) from Streptomyces sp. KCTC 0041BP.
  Journal
Glycobiology 17:119-26 (2007)
DOI:10.1093/glycob/cwl060
  Sequence
[ag:ABB52524]
Reference
3  [PMID:23116432]
  Authors
Kubiak RL, Phillips RK, Zmudka MW, Ahn MR, Maka EM, Pyeatt GL, Roggensack SJ, Holden HM
  Title
Structural and functional studies on a 3'-epimerase involved in the biosynthesis  of dTDP-6-deoxy-D-allose.
  Journal
Biochemistry 51:9375-83 (2012)
DOI:10.1021/bi3012737
Other DBs
ExplorEnz - The Enzyme Database: 5.1.3.27
IUBMB Enzyme Nomenclature: 5.1.3.27
ExPASy - ENZYME nomenclature database: 5.1.3.27
BRENDA, the Enzyme Database: 5.1.3.27
LinkDB

KEGG   REACTION: R06437
Entry
R06437                      Reaction                               
Name
dTDP-4-dehydro-6-deoxy-alpha-D-glucose 3-epimerase
Definition
dTDP-4-oxo-6-deoxy-D-glucose <=> dTDP-4-oxo-6-deoxy-D-allose
Equation
C11907 <=> C11926
Reaction class
RC01519  C11907_C11926
Enzyme
5.1.3.27
Pathway
rn00523  Polyketide sugar unit biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
rn01250  Biosynthesis of nucleotide sugars
Module
M00794  dTDP-6-deoxy-D-allose biosynthesis
M00795  dTDP-beta-L-noviose biosynthesis
Orthology
K13312  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase [EC:5.1.3.27]
K19855  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase [EC:5.1.3.27]
Other DBs
RHEA: 36974
LinkDB

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