KEGG   ORTHOLOGY: K13384
Entry
K13384                      KO                                     
Symbol
CNMT
Name
(RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline N-methyltransferase [EC:2.1.1.115 2.1.1.140]
Pathway
map00950  Isoquinoline alkaloid biosynthesis
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00943  Reticuline biosynthesis, dopamine + 4HPAA => (S)-reticuline
Reaction
R04692  S-adenosyl-L-methionine:(S)-coclaurine-N-methyltransferase
R05211  S-adenosyl-L-methionine:(RS)-1-benzyl-1,2,3,4-tetrahydroisoquinolie N- methyltransferase
R05216  S-adenosyl-L-methionine:(R)-norreticuline N- methyltransferase
R08794  
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00950 Isoquinoline alkaloid biosynthesis
    K13384  CNMT; (RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline N-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.115  (RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline N-methyltransferase
     K13384  CNMT; (RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline N-methyltransferase
    2.1.1.140  (S)-coclaurine-N-methyltransferase
     K13384  CNMT; (RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline N-methyltransferase
Other DBs
GO: 0030776 0030794
Genes
PSOM: 113294670 113318496
AG: AAU20766 BAB71802(CNMT)
Reference
PMID:11682473
  Authors
Choi KB, Morishige T, Shitan N, Yazaki K, Sato F
  Title
Molecular cloning and characterization of coclaurine N-methyltransferase from cultured cells of Coptis japonica.
  Journal
J Biol Chem 277:830-5 (2002)
DOI:10.1074/jbc.M106405200
  Sequence
[ag:BAB71802]
Reference
PMID:19624470
  Authors
Liscombe DK, Ziegler J, Schmidt J, Ammer C, Facchini PJ
  Title
Targeted metabolite and transcript profiling for elucidating enzyme function: isolation of novel N-methyltransferases from three benzylisoquinoline alkaloid-producing species.
  Journal
Plant J 60:729-43 (2009)
DOI:10.1111/j.1365-313X.2009.03980.x
LinkDB

KEGG   ENZYME: 2.1.1.140
Entry
EC 2.1.1.140                Enzyme                                 
Name
(S)-coclaurine-N-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:(S)-coclaurine-N-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + (S)-coclaurine = S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine [RN:R04692]
Reaction(KEGG)
R04692
Substrate
S-adenosyl-L-methionine [CPD:C00019];
(S)-coclaurine [CPD:C06161]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
(S)-N-methylcoclaurine [CPD:C05176]
Comment
The enzyme is specific for the (S)-isomer of coclaurine. Norcoclaurine can also act as an acceptor.
History
EC 2.1.1.140 created 2001
Pathway
ec00950  Isoquinoline alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13384  (RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline N-methyltransferase
Genes
PSOM: 113294670 113318496
Reference
1
  Authors
Loeffler S, Deus-Neumann B, Zenk MH.
  Title
S-Adenosyl-L-methionine: (S)-coclaurine-N-methyltransferase from Tinospora cordifolia.
  Journal
Phytochemistry 38:1387-1395 (1995)
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.140
IUBMB Enzyme Nomenclature: 2.1.1.140
ExPASy - ENZYME nomenclature database: 2.1.1.140
BRENDA, the Enzyme Database: 2.1.1.140
CAS: 167398-06-3
LinkDB

KEGG   REACTION: R04692
Entry
R04692                      Reaction                               
Name
S-adenosyl-L-methionine:(S)-coclaurine-N-methyltransferase
Definition
S-Adenosyl-L-methionine + (S)-Coclaurine <=> S-Adenosyl-L-homocysteine + (S)-N-Methylcoclaurine + H+
Equation
C00019 + C06161 <=> C00021 + C05176 + C00080
Reaction class
RC00003  C00019_C00021
RC01198  C05176_C06161
Enzyme
2.1.1.115       2.1.1.140
Pathway
rn00950  Isoquinoline alkaloid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00943  Reticuline biosynthesis, dopamine + 4HPAA => (S)-reticuline
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.115
     R04692  S-Adenosyl-L-methionine + (S)-Coclaurine <=> S-Adenosyl-L-homocysteine + (S)-N-Methylcoclaurine + H+
    2.1.1.140
     R04692  S-Adenosyl-L-methionine + (S)-Coclaurine <=> S-Adenosyl-L-homocysteine + (S)-N-Methylcoclaurine + H+
Orthology
K13384  (RS)-1-benzyl-1,2,3,4-tetrahydroisoquinoline N-methyltransferase [EC:2.1.1.115 2.1.1.140]
Other DBs
RHEA: 17412
LinkDB

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