KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
09110 Biosynthesis of other secondary metabolites
00950 Isoquinoline alkaloid biosynthesis
K13387 CYP80A1; berbamunine synthase
09180 Brite Hierarchies
09181 Protein families: metabolism
00199 Cytochrome P450
K13387 CYP80A1; berbamunine synthase
Enzymes [BR:ko01000]
1. Oxidoreductases
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
1.14.19.66 berbamunine synthase
K13387 CYP80A1; berbamunine synthase
Cytochrome P450 [BR:ko00199]
Cytochrome P450, plant type
CYP80 family
K13387 CYP80A1; berbamunine synthase
Molecular cloning and heterologous expression of a cDNA encoding berbamunine synthase, a C--O phenol-coupling cytochrome P450 from the higher plant Berberis stolonifera.
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
A cytochrome P-450 (heme-thiolate) protein found in plants. Forms the bisbenzylisoquinoline alkaloid berbamunine by phenol oxidation of N-methylcoclaurine without the incorporation of oxygen into the product. Reaction of two molecules of (R)-N-methylcoclaurine gives the dimer guattagaumerine.
History
EC 1.14.19.66 created 1999 as EC 1.1.3.34, transferred 2002 to EC 1.14.21.3, transferred 2018 to EC 1.14.19.66
Enzymatic reactions [BR:br08201]
1. Oxidoreductase reactions
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.19 With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
1.14.19.66
R05215 2 (R)-N-Methylcoclaurine + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> Guattegaumerine + [Oxidized NADPH---hemoprotein reductase] + 2 H2O
