KEGG   ORTHOLOGY: K13398
Entry
K13398            Tight     KO                                     
Symbol
E2.1.1.118
Name
columbamine O-methyltransferase [EC:2.1.1.118]
Pathway
map00950  Isoquinoline alkaloid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R03721  S-adenosyl-L-methionine:columbamine O-methyltransferase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00950 Isoquinoline alkaloid biosynthesis
    K13398  E2.1.1.118; columbamine O-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.118  columbamine O-methyltransferase
     K13398  E2.1.1.118; columbamine O-methyltransferase
Other DBs
GO: 0030778
Genes
AG: BAC22084
Reference
PMID:12423366
  Authors
Morishige T, Dubouzet E, Choi KB, Yazaki K, Sato F
  Title
Molecular cloning of columbamine O-methyltransferase from cultured Coptis japonica cells.
  Journal
Eur J Biochem 269:5659-67 (2002)
DOI:10.1046/j.1432-1033.2002.03275.x
  Sequence
[ag:BAC22084]
LinkDB

KEGG   ENZYME: 2.1.1.118
Entry
EC 2.1.1.118                Enzyme                                 
Name
columbamine O-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:columbamine O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + columbamine = S-adenosyl-L-homocysteine + palmatine [RN:R03721]
Reaction(KEGG)
R03721
Substrate
S-adenosyl-L-methionine [CPD:C00019];
columbamine [CPD:C01795]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
palmatine [CPD:C05315]
Comment
The product of this reaction is a protoberberine alkaloid that is widely distributed in the plant kingdom. This enzyme is distinct in specificity from EC 2.1.1.88, 8-hydroxyquercetin 8-O-methyltransferase.
History
EC 2.1.1.118 created 1999
Pathway
ec00950  Isoquinoline alkaloid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13398  columbamine O-methyltransferase
Reference
1
  Authors
Rueffer M, Amann M, Zenk MH.
  Title
S-Adenosyl-L-methionine:columbamine O-methyltransferase, a compartmentalized enzyme in protoberberine biosynthesis.
  Journal
Plant Cell Reports 3:182-185 (1986)
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.118
IUBMB Enzyme Nomenclature: 2.1.1.118
ExPASy - ENZYME nomenclature database: 2.1.1.118
BRENDA, the Enzyme Database: 2.1.1.118
CAS: 105843-76-3
LinkDB

KEGG   REACTION: R03721
Entry
R03721                      Reaction                               
Name
S-adenosyl-L-methionine:columbamine O-methyltransferase
Definition
S-Adenosyl-L-methionine + Columbamine <=> S-Adenosyl-L-homocysteine + Palmatine
Equation
C00019 + C01795 <=> C00021 + C05315
Reaction class
RC00003  C00019_C00021
RC00392  C01795_C05315
Enzyme
2.1.1.118
Pathway
rn00950  Isoquinoline alkaloid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.118
     R03721  S-Adenosyl-L-methionine + Columbamine <=> S-Adenosyl-L-homocysteine + Palmatine
Orthology
K13398  columbamine O-methyltransferase [EC:2.1.1.118]
Other DBs
RHEA: 15376
LinkDB

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