KEGG   ORTHOLOGY: K14028
Entry
K14028                      KO                                     

Name
mdh1, mxaF
Definition
methanol dehydrogenase (cytochrome c) subunit 1 [EC:1.1.2.7]
Pathway
ko00010  Glycolysis / Gluconeogenesis
ko00620  Pyruvate metabolism
ko00625  Chloroalkane and chloroalkene degradation
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00010 Glycolysis / Gluconeogenesis
    K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
   00620 Pyruvate metabolism
    K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
  09102 Energy metabolism
   00680 Methane metabolism
    K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
  09111 Xenobiotics biodegradation and metabolism
   00625 Chloroalkane and chloroalkene degradation
    K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.2  With a cytochrome as acceptor
    1.1.2.7  methanol dehydrogenase (cytochrome c)
     K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
Other DBs
RN: R01146 R09127 R09128 R10713
GO: 0052930
Genes
DKO: I596_1067
MCA: MCA0779(mxaF)
METU: GNH96_04380
MMT: Metme_4317
MDN: JT25_020965
MDH: AYM39_15615
MKO: MKLM6_3210
METL: U737_12050
MAH: MEALZ_3448(mxaF)
MBUR: EQU24_18145
MMAI: sS8_1436
MEJ: Q7A_537
MEC: Q7C_2125
VEI: Veis_1829
MFA: Mfla_2044
MEP: MPQ_0771
RPC: RPC_1907
RPE: RPE_3139
SNO: Snov_4188
MEA: Mex_1p4538(mxaF)
MDI: METDI5145(mxaF)
MEX: Mext_4150
MCH: Mchl_4518
MPO: Mpop_4632
MNO: Mnod_8040
MOR: MOC_4862(mxaF)
META: Y590_20685
MAQU: Maq22A_1p33165(mxaF)
MSL: Msil_0471
MTUN: MTUNDRAET4_1612(mxaF)
HDN: Hden_1323
HMC: HYPMC_0485(mxaF)
FIL: BN1229_v1_2515(mxaF)
FIY: BN1229_v1_3401(mxaF)
MCG: GL4_0421
MSC: BN69_2570(mxaF)
PLEO: OHA_1_04073(mxaF)
PDE: Pden_2993
ALI: AZOLI_p10948(moxF1)
MOX: DAMO_0112(mxaF)
 » show all
Reference
PMID:1311606
  Authors
Cox JM, Day DJ, Anthony C
  Title
The interaction of methanol dehydrogenase and its electron acceptor, cytochrome cL in methylotrophic bacteria.
  Journal
Biochim Biophys Acta 1119:97-106 (1992)
DOI:10.1016/0167-4838(92)90240-E
Reference
PMID:7656012
  Authors
Blake CC, Ghosh M, Harlos K, Avezoux A, Anthony C
  Title
The active site of methanol dehydrogenase contains a disulphide bridge between adjacent cysteine residues.
  Journal
Nat Struct Biol 1:102-5 (1994)
DOI:10.1038/nsb0294-102
  Sequence
LinkDB

KEGG   ORTHOLOGY: K14029
Entry
K14029                      KO                                     

Name
mdh2, mxaI
Definition
methanol dehydrogenase (cytochrome c) subunit 2 [EC:1.1.2.7]
Pathway
ko00010  Glycolysis / Gluconeogenesis
ko00620  Pyruvate metabolism
ko00625  Chloroalkane and chloroalkene degradation
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00010 Glycolysis / Gluconeogenesis
    K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
   00620 Pyruvate metabolism
    K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
  09102 Energy metabolism
   00680 Methane metabolism
    K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
  09111 Xenobiotics biodegradation and metabolism
   00625 Chloroalkane and chloroalkene degradation
    K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.2  With a cytochrome as acceptor
    1.1.2.7  methanol dehydrogenase (cytochrome c)
     K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
Other DBs
RN: R01146 R09127 R09128 R10713
GO: 0052930
Genes
DKO: I596_1064
MCA: MCA0782(mxaI)
METU: GNH96_04395
MMT: Metme_4320
MDN: JT25_020980
MDH: AYM39_15600
MKO: MKLM6_3207
METL: U737_12035
MAH: MEALZ_3445(mxaI)
MBUR: EQU24_18130
MMAI: sS8_1433
MEJ: Q7A_534
MEC: Q7C_2122
VEI: Veis_1826
MFA: Mfla_2041
MEP: MPQ_0774
RPC: RPC_1910
RPE: RPE_3136
SNO: Snov_4191
MEA: Mex_1p4535(mxaI)
MDI: METDI5141(mxaI)
MEX: Mext_4147
MCH: Mchl_4515
MPO: Mpop_4629
MNO: Mnod_8037
MOR: MOC_4859
META: Y590_20670
MAQU: Maq22A_1p33180(mxaI)
MSL: Msil_0474
MTUN: MTUNDRAET4_1609(mxaI)
HDN: Hden_1320
HMC: HYPMC_0488(mxaI)
FIL: BN1229_v1_2518(mxaI)
FIY: BN1229_v1_3398(mxaI)
MCG: GL4_0424
MSC: BN69_2573
PLEO: OHA_1_04076(mxaI)
PDE: Pden_2996
ALI: AZOLI_p10951(moxI)
MANY: MANY_50940
MOX: DAMO_0115(mxaI)
 » show all
Reference
PMID:1311606
  Authors
Cox JM, Day DJ, Anthony C
  Title
The interaction of methanol dehydrogenase and its electron acceptor, cytochrome cL in methylotrophic bacteria.
  Journal
Biochim Biophys Acta 1119:97-106 (1992)
DOI:10.1016/0167-4838(92)90240-E
Reference
PMID:7656012
  Authors
Blake CC, Ghosh M, Harlos K, Avezoux A, Anthony C
  Title
The active site of methanol dehydrogenase contains a disulphide bridge between adjacent cysteine residues.
  Journal
Nat Struct Biol 1:102-5 (1994)
DOI:10.1038/nsb0294-102
  Sequence
LinkDB

KEGG   ENZYME: 1.1.2.7
Entry
EC 1.1.2.7                  Enzyme                                 

Name
methanol dehydrogenase (cytochrome c);
methanol dehydrogenase;
MDH (ambiguous)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With a cytochrome as acceptor
Sysname
methanol:cytochrome c oxidoreductase
Reaction(IUBMB)
a primary alcohol + 2 ferricytochrome cL = an aldehyde + 2 ferrocytochrome cL + 2 H+ [RN:R10713]
Reaction(KEGG)
Substrate
primary alcohol [CPD:C00226];
ferricytochrome cL [CPD:C18233]
Product
aldehyde [CPD:C00071];
ferrocytochrome cL [CPD:C18234];
H+ [CPD:C00080]
Comment
A periplasmic quinoprotein alcohol dehydrogenase that only occurs in methylotrophic bacteria. It uses the novel specific cytochrome cL as acceptor. Acts on a wide range of primary alcohols, including ethanol, duodecanol, chloroethanol, cinnamyl alcohol, and also formaldehyde. Activity is stimulated by ammonia or methylamine. It is usually assayed with phenazine methosulfate. Like all other quinoprotein alcohol dehydrogenases it has an 8-bladed 'propeller' structure, a calcium ion bound to the PQQ in the active site and an unusual disulfide ring structure in close proximity to the PQQ. It differs from EC 1.1.2.8, alcohol dehydrogenase (cytochrome c), in having a high affinity for methanol and in having a second essential small subunit (no known function).
History
EC 1.1.2.7 created 1972 as EC 1.1.99.8, modified 1982, part transferred 2010 to EC 1.1.2.7
Pathway
ec00010  Glycolysis / Gluconeogenesis
ec00620  Pyruvate metabolism
ec00625  Chloroalkane and chloroalkene degradation
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01120  Microbial metabolism in diverse environments
Orthology
K14028  methanol dehydrogenase (cytochrome c) subunit 1
K14029  methanol dehydrogenase (cytochrome c) subunit 2
Genes
DKO: I596_1064 I596_1067
MCA: MCA0779(mxaF) MCA0782(mxaI)
METU: GNH96_04380 GNH96_04395
MMT: Metme_4317 Metme_4320
MDN: JT25_020965 JT25_020980
MDH: AYM39_15600 AYM39_15615
MKO: MKLM6_3207 MKLM6_3210
METL: U737_12035 U737_12050
MAH: MEALZ_3445(mxaI) MEALZ_3448(mxaF)
MBUR: EQU24_18130 EQU24_18145
MEA: Mex_1p4535(mxaI) Mex_1p4538(mxaF)
MDI: METDI5141(mxaI) METDI5145(mxaF)
MOR: MOC_4859 MOC_4862(mxaF)
MAQU: Maq22A_1p33165(mxaF) Maq22A_1p33180(mxaI)
MTUN: MTUNDRAET4_1609(mxaI) MTUNDRAET4_1612(mxaF)
HMC: HYPMC_0485(mxaF) HYPMC_0488(mxaI)
FIL: BN1229_v1_2515(mxaF) BN1229_v1_2518(mxaI)
FIY: BN1229_v1_3398(mxaI) BN1229_v1_3401(mxaF)
MSC: BN69_2570(mxaF) BN69_2573
PLEO: OHA_1_04073(mxaF) OHA_1_04076(mxaI)
ALI: AZOLI_p10948(moxF1) AZOLI_p10951(moxI)
MANY: MANY_50940
MOX: DAMO_0112(mxaF) DAMO_0115(mxaI)
 » show all
Reference
1  [PMID:4378696]
  Authors
Anthony C, Zatman LJ
  Title
The microbial oxidation of methanol. 2. The methanol-oxidizing enzyme of Pseudomonas sp. M 27.
  Journal
Biochem J 92:614-21 (1964)
DOI:10.1042/bj0920614
Reference
2  [PMID:6049934]
  Authors
Anthony C, Zatman LJ
  Title
The microbial oxidation of methanol. The prosthetic group of the alcohol dehydrogenase of Pseudomonas sp. M27: a new oxidoreductase prosthetic group.
  Journal
Biochem J 104:960-9 (1967)
DOI:10.1042/bj1040960
Reference
3  [PMID:6250827]
  Authors
Duine JA, Frank J, Verwiel PE.
  Title
Structure and activity of the prosthetic group of methanol dehydrogenase.
  Journal
Eur J Biochem 108:187-92 (1980)
DOI:10.1111/j.1432-1033.1980.tb04711.x
Reference
4  [PMID:471057]
  Authors
Salisbury SA, Forrest HS, Cruse WB, Kennard O.
  Title
A novel coenzyme from bacterial primary alcohol dehydrogenases.
  Journal
Nature 280:843-4 (1979)
DOI:10.1038/280843a0
Reference
5  [PMID:1311606]
  Authors
Cox JM, Day DJ, Anthony C
  Title
The interaction of methanol dehydrogenase and its electron acceptor, cytochrome cL in methylotrophic bacteria.
  Journal
Biochim Biophys Acta 1119:97-106 (1992)
DOI:10.1016/0167-4838(92)90240-E
  Sequence
Reference
6  [PMID:7656012]
  Authors
Blake CC, Ghosh M, Harlos K, Avezoux A, Anthony C
  Title
The active site of methanol dehydrogenase contains a disulphide bridge between adjacent cysteine residues.
  Journal
Nat Struct Biol 1:102-5 (1994)
DOI:10.1038/nsb0294-102
  Sequence
Reference
7  [PMID:9930981]
  Authors
Xia ZX, He YN, Dai WW, White SA, Boyd GD, Mathews FS
  Title
Detailed active site configuration of a new crystal form of methanol dehydrogenase from Methylophilus W3A1 at 1.9 A resolution.
  Journal
Biochemistry 38:1214-20 (1999)
DOI:10.1021/bi9822574
Reference
8  [PMID:11502173]
  Authors
Afolabi PR, Mohammed F, Amaratunga K, Majekodunmi O, Dales SL, Gill R, Thompson D, Cooper JB, Wood SP, Goodwin PM, Anthony C
  Title
Site-directed mutagenesis and X-ray crystallography of the PQQ-containing quinoprotein methanol dehydrogenase and its electron acceptor, cytochrome c(L).
  Journal
Biochemistry 40:9799-809 (2001)
DOI:10.1021/bi002932l
  Sequence
Reference
9  [PMID:12686102]
  Authors
Anthony C, Williams P
  Title
The structure and mechanism of methanol dehydrogenase.
  Journal
Biochim Biophys Acta 1647:18-23 (2003)
DOI:10.1016/S1570-9639(03)00042-6
Reference
10 [PMID:15608378]
  Authors
Williams PA, Coates L, Mohammed F, Gill R, Erskine PT, Coker A, Wood SP, Anthony C, Cooper JB
  Title
The atomic resolution structure of methanol dehydrogenase from Methylobacterium extorquens.
  Journal
Acta Crystallogr D Biol Crystallogr 61:75-9 (2005)
DOI:10.1107/S0907444904026964
Other DBs
ExplorEnz - The Enzyme Database: 1.1.2.7
IUBMB Enzyme Nomenclature: 1.1.2.7
ExPASy - ENZYME nomenclature database: 1.1.2.7
UM-BBD (Biocatalysis/Biodegradation Database): 1.1.2.7
BRENDA, the Enzyme Database: 1.1.2.7
CAS: 37205-43-9
LinkDB

KEGG   REACTION: R09127
Entry
R09127                      Reaction                               

Name
ethanol:cytochrome c oxidoreductase
Definition
Ethanol + 2 Ferricytochrome cL <=> Acetaldehyde + 2 Ferrocytochrome cL + 2 H+
Equation
C00469 + 2 C18233 <=> C00084 + 2 C18234 + 2 C00080
Reaction class
RC00088  C00084_C00469
Enzyme
Pathway
rn00010  Glycolysis / Gluconeogenesis
rn00620  Pyruvate metabolism
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
rn01120  Microbial metabolism in diverse environments
Orthology
K14028  methanol dehydrogenase (cytochrome c) subunit 1 [EC:1.1.2.7]
K14029  methanol dehydrogenase (cytochrome c) subunit 2 [EC:1.1.2.7]
LinkDB

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