KEGG   ORTHOLOGY: K14038
Entry
K14038                      KO                                     
Symbol
KSL5
Name
ent-pimara-8(14),15-diene synthase [EC:4.2.3.30]
Pathway
map00904  Diterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R09121  ent-copalyl-diphosphate diphosphate-lyase [ent-pimara-8(14),15-diene-forming]
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K14038  KSL5; ent-pimara-8(14),15-diene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.30  ent-pimara-8(14),15-diene synthase
     K14038  KSL5; ent-pimara-8(14),15-diene synthase
Other DBs
GO: 0034282
Genes
OSA: 4329728(KS6)
OGL: 127761379
Reference
PMID:16861806
  Authors
Kanno Y, Otomo K, Kenmoku H, Mitsuhashi W, Yamane H, Oikawa H, Toshima H, Matsuoka M, Sassa T, Toyomasu T
  Title
Characterization of a rice gene family encoding type-A diterpene cyclases.
  Journal
Biosci Biotechnol Biochem 70:1702-10 (2006)
DOI:10.1271/bbb.60044
  Sequence
[osa:4329728]
Reference
PMID:17986769
  Authors
Yajima A, Yamaguchi A, Nukada T, Yabuta G
  Title
Asymmetric total synthesis of ent-sandaracopimaradiene, a biosynthetic intermediate of oryzalexins.
  Journal
Biosci Biotechnol Biochem 71:2822-9 (2007)
DOI:10.1271/bbb.70468
LinkDB

KEGG   ENZYME: 4.2.3.30
Entry
EC 4.2.3.30                 Enzyme                                 
Name
ent-pimara-8(14),15-diene synthase;
OsKS5
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
ent-copalyl-diphosphate diphosphate-lyase [ent-pimara-8(14),15-diene-forming]
Reaction(IUBMB)
ent-copalyl diphosphate = ent-pimara-8(14),15-diene + diphosphate [RN:R09121]
Reaction(KEGG)
R09121
Substrate
ent-copalyl diphosphate [CPD:C06089]
Product
ent-pimara-8(14),15-diene [CPD:C18228];
diphosphate [CPD:C00013]
Comment
Unlike EC 4.2.3.29, ent-sandaracopimaradiene synthase, which can produce both ent-sandaracopimaradiene and ent-pimara-8(14),15-diene, this diterpene cyclase produces only ent-pimara-8(14),15-diene. ent-Pimara-8(14),15-diene is not a precursor in the biosynthesis of either gibberellins or phytoalexins.
History
EC 4.2.3.30 created 2008
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K14038  ent-pimara-8(14),15-diene synthase
Genes
OSA: 4329728(KS6)
OGL: 127761379
Reference
1  [PMID:16861806]
  Authors
Kanno Y, Otomo K, Kenmoku H, Mitsuhashi W, Yamane H, Oikawa H, Toshima H, Matsuoka M, Sassa T, Toyomasu T
  Title
Characterization of a rice gene family encoding type-A diterpene cyclases.
  Journal
Biosci Biotechnol Biochem 70:1702-10 (2006)
DOI:10.1271/bbb.60044
  Sequence
[osa:4329728]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.30
IUBMB Enzyme Nomenclature: 4.2.3.30
ExPASy - ENZYME nomenclature database: 4.2.3.30
BRENDA, the Enzyme Database: 4.2.3.30
LinkDB

KEGG   REACTION: R09121
Entry
R09121                      Reaction                               
Name
ent-copalyl-diphosphate diphosphate-lyase [ent-pimara-8(14),15-diene-forming]
Definition
ent-Copalyl diphosphate <=> ent-Pimara-8(14),15-diene + Diphosphate
Equation
C06089 <=> C18228 + C00013
Reaction class
RC01494  C06089_C18228
Enzyme
4.2.3.30
Pathway
rn00904  Diterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.30
     R09121  ent-Copalyl diphosphate <=> ent-Pimara-8(14),15-diene + Diphosphate
Orthology
K14038  ent-pimara-8(14),15-diene synthase [EC:4.2.3.30]
Other DBs
RHEA: 25543
LinkDB

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