KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
09109 Metabolism of terpenoids and polyketides
00904 Diterpenoid biosynthesis
K14040 CYP720B, PTAO; abieta-7,13-dien-18-ol hydroxylase
09180 Brite Hierarchies
09181 Protein families: metabolism
00199 Cytochrome P450
K14040 CYP720B, PTAO; abieta-7,13-dien-18-ol hydroxylase
Enzymes [BR:ko01000]
1. Oxidoreductases
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
1.14.14.145 abieta-7,13-dien-18-ol hydroxylase
K14040 CYP720B, PTAO; abieta-7,13-dien-18-ol hydroxylase
Cytochrome P450 [BR:ko00199]
Cytochrome P450, plant type
CYP720 family
K14040 CYP720B, PTAO; abieta-7,13-dien-18-ol hydroxylase
Diterpene resin acid biosynthesis in loblolly pine (Pinus taeda): functional characterization of abietadiene/levopimaradiene synthase (PtTPS-LAS) cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase (PtAO, CYP720B1).
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
A cytochrome P-450 (heme-thiolate) protein. This enzyme catalyses a step in the pathway of abietic acid biosynthesis. The activity has been demonstrated in cell-free stem extracts of Abies grandis (grand fir) and Pinus contorta (lodgepole pine) [1], and the gene encoding the enzyme has been identified in Pinus taeda (loblolly pine) [3]. The recombinant enzyme catalyses the oxidation of multiple diterpene alcohol and aldehydes, including levopimaradienol, isopimara-7,15-dienol, isopimara-7,15-dienal, dehydroabietadienol and dehydroabietadienal. It is not able to oxidize abietadiene.
History
EC 1.14.14.145 created 2009 as EC 1.14.13.109, modified 2012, transferred 2018 to EC 1.14.14.145
Diterpenoid resin acid biosynthesis in conifers: characterization of two cytochrome P450-dependent monooxygenases and an aldehyde dehydrogenase involved in abietic acid biosynthesis.
Funk C, Lewinsohn E, Vogel BS, Steele CL, Croteau R
Title
Regulation of Oleoresinosis in Grand Fir (Abies grandis) (Coordinate Induction of Monoterpene and Diterpene Cyclases and Two Cytochrome P450-Dependent Diterpenoid Hydroxylases by Stem Wounding).
Enzymatic reactions [BR:br08201]
1. Oxidoreductase reactions
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
1.14.14.145
R06354 Abietinol + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> Abietal + [Oxidized NADPH---hemoprotein reductase] + 2 H2O