KEGG   ORTHOLOGY: K14044
Entry
K14044                      KO                                     
Symbol
KSL8, DTC2
Name
stemar-13-ene synthase [EC:4.2.3.33]
Pathway
map00904  Diterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R09115  9alpha-copalyl-diphosphate diphosphate-lyase (stemar-13-ene-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K14044  KSL8, DTC2; stemar-13-ene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.33  stemar-13-ene synthase
     K14044  KSL8, DTC2; stemar-13-ene synthase
Other DBs
GO: 0034278
Genes
OSA: 136354096
DOSA: Os11g0474800
OBR: 102711108
Reference
PMID:15280039
  Authors
Nemoto T, Cho EM, Okada A, Okada K, Otomo K, Kanno Y, Toyomasu T, Mitsuhashi W, Sassa T, Minami E, Shibuya N, Nishiyama M, Nojiri H, Yamane H
  Title
Stemar-13-ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin S in rice.
  Journal
FEBS Lett 571:182-6 (2004)
DOI:10.1016/j.febslet.2004.07.002
  Sequence
[osa:136354096]
LinkDB

KEGG   ENZYME: 4.2.3.33
Entry
EC 4.2.3.33                 Enzyme                                 
Name
stemar-13-ene synthase;
OsDTC2;
OsK8;
OsKL8;
OsKS8;
stemarene synthase;
syn-stemar-13-ene synthase
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
9alpha-copalyl-diphosphate diphosphate-lyase (stemar-13-ene-forming)
Reaction(IUBMB)
9alpha-copalyl diphosphate = stemar-13-ene + diphosphate [RN:R09115]
Reaction(KEGG)
R09115
Substrate
9alpha-copalyl diphosphate [CPD:C11902]
Product
stemar-13-ene [CPD:C18223];
diphosphate [CPD:C00013]
Comment
This diterpene cyclase produces stemar-13-ene, a putative precursor of the rice phytoalexin oryzalexin S. Phytoalexins are diterpenoid secondary metabolites that are involved in the defense mechanism of the plant, and are produced in response to pathogen attack through the perception of elicitor signal molecules such as chitin oligosaccharide, or after exposure to UV irradiation.
History
EC 4.2.3.33 created 2008
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K14044  stemar-13-ene synthase
Genes
OSA: 136354096
DOSA: Os11g0474800
OBR: 102711108
Reference
1  [PMID:8651702]
  Authors
Mohan RS, Yee NK, Coates RM, Ren YY, Stamenkovic P, Mendez I, West CA.
  Title
Biosynthesis of cyclic diterpene hydrocarbons in rice cell suspensions: conversion of 9,10-syn-labda-8(17),13-dienyl diphosphate to 9beta-pimara-7,15-diene and stemar-13-ene.
  Journal
Arch Biochem Biophys 330:33-47 (1996)
DOI:10.1006/abbi.1996.0223
Reference
2  [PMID:15280039]
  Authors
Nemoto T, Cho EM, Okada A, Okada K, Otomo K, Kanno Y, Toyomasu T, Mitsuhashi W, Sassa T, Minami E, Shibuya N, Nishiyama M, Nojiri H, Yamane H
  Title
Stemar-13-ene synthase, a diterpene cyclase involved in the biosynthesis of the phytoalexin oryzalexin S in rice.
  Journal
FEBS Lett 571:182-6 (2004)
DOI:10.1016/j.febslet.2004.07.002
  Sequence
[osa:136354096]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.33
IUBMB Enzyme Nomenclature: 4.2.3.33
ExPASy - ENZYME nomenclature database: 4.2.3.33
BRENDA, the Enzyme Database: 4.2.3.33
LinkDB

KEGG   REACTION: R09115
Entry
R09115                      Reaction                               
Name
9alpha-copalyl-diphosphate diphosphate-lyase (stemar-13-ene-forming)
Definition
9alpha-Copalyl diphosphate <=> Stemar-13-ene + Diphosphate
Equation
C11902 <=> C18223 + C00013
Reaction class
RC02435  C11902_C18223
Enzyme
4.2.3.33
Pathway
rn00904  Diterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.33
     R09115  9alpha-Copalyl diphosphate <=> Stemar-13-ene + Diphosphate
Orthology
K14044  stemar-13-ene synthase [EC:4.2.3.33]
Other DBs
RHEA: 25555
LinkDB

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