KEGG   ORTHOLOGY: K14046
Entry
K14046                      KO                                     
Symbol
KSL4, DTS2
Name
syn-pimara-7,15-diene synthase [EC:4.2.3.35]
Pathway
map00904  Diterpenoid biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R09117  9alpha-copalyl-diphosphate diphosphate-lyase (9beta-pimara-7,15-diene-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K14046  KSL4, DTS2; syn-pimara-7,15-diene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.35  syn-pimara-7,15-diene synthase
     K14046  KSL4, DTS2; syn-pimara-7,15-diene synthase
Other DBs
GO: 0034279
Genes
OSA: 4335094(DTS2)
DOSA: Os04g0179700
OGL: 127771244
TAES: 100873130 100873132 123040055 123040057 123048259 123055484 123184297 123184304
TUA: 125531377 125535131
HVG: 123429122
PAUA: 133899288
AG: AAU05906(DTS2)
Reference
  Authors
Wilderman PR, Xu M, Jin Y, Coates RM, Peters RJ
  Title
Identification of syn-pimara-7,15-diene synthase reveals functional clustering of terpene synthases involved in rice phytoalexin/allelochemical biosynthesis.
  Journal
Plant Physiol 135:2098-105 (2004)
DOI:10.1104/pp.104.045971
  Sequence
LinkDB

KEGG   ENZYME: 4.2.3.35
Entry
EC 4.2.3.35                 Enzyme                                 
Name
syn-pimara-7,15-diene synthase;
9beta-pimara-7,15-diene synthase;
OsDTS2;
OsKS4
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
9alpha-copalyl-diphosphate diphosphate-lyase (9beta-pimara-7,15-diene-forming)
Reaction(IUBMB)
9alpha-copalyl diphosphate = 9beta-pimara-7,15-diene + diphosphate [RN:R09117]
Reaction(KEGG)
R09117
Substrate
9alpha-copalyl diphosphate [CPD:C11902]
Product
9beta-pimara-7,15-diene [CPD:C18225];
diphosphate [CPD:C00013]
Comment
This enzyme is a class I terpene synthase [1]. 9beta-Pimara-7,15-diene is a precursor of momilactones A and B, rice diterpenoid phytoalexins that are produced in response to attack (by a pathogen, elicitor or UV irradiation) and are involved in the defense mechanism of the plant. Momilactone B can also act as an allochemical, being constitutively produced in the root of the plant and secreted to the rhizosphere where it suppresses the growth of neighbouring plants and soil microorganisms [1].
History
EC 4.2.3.35 created 2008
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K14046  syn-pimara-7,15-diene synthase
Genes
OSA4335094(DTS2)
DOSAOs04g0179700
OGL127771244
TAES100873130 100873132 123040055 123040057 123048259 123055484 123184297 123184304
TUA125531377 125535131
HVG123429122
PAUA133899288
Reference
1  [PMID:15299118]
  Authors
Wilderman PR, Xu M, Jin Y, Coates RM, Peters RJ
  Title
Identification of syn-pimara-7,15-diene synthase reveals functional clustering of terpene synthases involved in rice phytoalexin/allelochemical biosynthesis.
  Journal
Plant Physiol 135:2098-105 (2004)
DOI:10.1104/pp.104.045971
  Sequence
Reference
2  [PMID:15388982]
  Authors
Otomo K, Kanno Y, Motegi A, Kenmoku H, Yamane H, Mitsuhashi W, Oikawa H, Toshima H, Itoh H, Matsuoka M, Sassa T, Toyomasu T
  Title
Diterpene cyclases responsible for the biosynthesis of phytoalexins, momilactones A, B, and oryzalexins A-F in rice.
  Journal
Biosci Biotechnol Biochem 68:2001-6 (2004)
DOI:10.1271/bbb.68.2001
  Sequence
[osa:4335094]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.35
IUBMB Enzyme Nomenclature: 4.2.3.35
ExPASy - ENZYME nomenclature database: 4.2.3.35
BRENDA, the Enzyme Database: 4.2.3.35
LinkDB

KEGG   REACTION: R09117
Entry
R09117                      Reaction                               
Name
9alpha-copalyl-diphosphate diphosphate-lyase (9beta-pimara-7,15-diene-forming)
Definition
9alpha-Copalyl diphosphate <=> 9beta-Pimara-7,15-diene + Diphosphate
Equation
Reaction class
RC02434  C11902_C18225
Enzyme
Pathway
rn00904  Diterpenoid biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 4. Lyase reactions
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.35
     R09117  9alpha-Copalyl diphosphate <=> 9beta-Pimara-7,15-diene + Diphosphate
Orthology
K14046  syn-pimara-7,15-diene synthase [EC:4.2.3.35]
Other DBs
RHEA: 25563
LinkDB

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