KEGG   ORTHOLOGY: K14244
Entry
K14244            Tight     KO                                     
Symbol
oxyD
Name
amidotransferase [EC:6.3.5.-]
Pathway
map00253  Tetracycline biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R09195  malonyl-CoA:L-glutamine amido-ligase (AMP-forming)
R11515  malonyl-[acyl-carrier protein]:L-glutamine amido-ligase (AMP-forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00253 Tetracycline biosynthesis
    K14244  oxyD; amidotransferase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K14244  oxyD; amidotransferase
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide tailoring proteins
  Others
   K14244  oxyD; amidotransferase
Genes
SRIM: CP984_02390(asnB)
SPLA: CP981_34515(asnB)
AG: AAZ78328(oxyD)
Reference
PMID:16597959
  Authors
Zhang W, Ames BD, Tsai SC, Tang Y
  Title
Engineered biosynthesis of a novel amidated polyketide, using the malonamyl-specific initiation module from the oxytetracycline polyketide synthase.
  Journal
Appl Environ Microbiol 72:2573-80 (2006)
DOI:10.1128/AEM.72.4.2573-2580.2006
  Sequence
[ag:AAZ78328]
Reference
PMID:20522541
  Authors
Pickens LB, Tang Y
  Title
Oxytetracycline biosynthesis.
  Journal
J Biol Chem 285:27509-15 (2010)
DOI:10.1074/jbc.R110.130419
LinkDB

KEGG   REACTION: R11515
Entry
R11515                      Reaction                               
Name
malonyl-[acyl-carrier protein]:L-glutamine amido-ligase (AMP-forming)
Definition
ATP + Malonyl-[acyl-carrier protein] + L-Glutamine + H2O <=> AMP + Diphosphate + Malonamoyl-[acyl-carrier protein] + L-Glutamate
Equation
C00002 + C01209 + C00064 + C00001 <=> C00020 + C00013 + C21430 + C00025
Comment
The substrate of OxyD can be either malonyl-CoA (see R09195) or malonyl-ACP.
Reaction class
RC00010  C00025_C00064  C01209_C21430
Enzyme
6.3.5.-
Pathway
rn00253  Tetracycline biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 6. Ligase reactions
  6.3  Forming carbon-nitrogen bonds
   6.3.5  Carbon-nitrogen ligases with glutamine as amido-N-donor
    6.3.5.-
     R11515  ATP + Malonyl-[acyl-carrier protein] + L-Glutamine + H2O <=> AMP + Diphosphate + Malonamoyl-[acyl-carrier protein] + L-Glutamate
Orthology
K14244  amidotransferase [EC:6.3.5.-]
LinkDB

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