KEGG   ORTHOLOGY: K14257
Entry
K14257                      KO                                     
Symbol
ctcP, cts4, prnC
Name
tetracycline 7-halogenase / FADH2 O2-dependent halogenase [EC:1.14.19.49 1.14.19.-]
Pathway
map00253  Tetracycline biosynthesis
map00404  Staurosporine biosynthesis
map01057  Biosynthesis of type II polyketide products
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00790  Pyrrolnitrin biosynthesis, tryptophan => pyrrolnitrin
M00823  Chlortetracycline biosynthesis, pretetramide => chlortetracycline
Reaction
R11106  
R11478  tetracycline:FADH2 oxidoreductase (7-halogenating)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K14257  ctcP, cts4, prnC; tetracycline 7-halogenase / FADH2 O2-dependent halogenase
   00253 Tetracycline biosynthesis
    K14257  ctcP, cts4, prnC; tetracycline 7-halogenase / FADH2 O2-dependent halogenase
  09110 Biosynthesis of other secondary metabolites
   00404 Staurosporine biosynthesis
    K14257  ctcP, cts4, prnC; tetracycline 7-halogenase / FADH2 O2-dependent halogenase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K14257  ctcP, cts4, prnC; tetracycline 7-halogenase / FADH2 O2-dependent halogenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.49  tetracycline 7-halogenase
     K14257  ctcP, cts4, prnC; tetracycline 7-halogenase / FADH2 O2-dependent halogenase
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide tailoring proteins
  Halogenase
   K14257  ctcP, cts4, prnC; tetracycline 7-halogenase / FADH2 O2-dependent halogenase
Other DBs
COG: COG0644
Genes
ECLN: ECNIH4_08285
ENO: ECENHK_14345
ECHG: FY206_15895
END: A4308_19035
ENZ: G0034_14940
SRR: SerAS9_4266
SRL: SOD_c39980(prnC)
SRY: M621_21735
SPLY: Q5A_021590(soxA)
SRS: SerAS12_4267
SRA: SerAS13_4267
SMAF: D781_2599
SRZ: AXX16_2518
SERM: CLM71_13480
SRHZ: FO014_20185
SGRI: SGBXF1_00335(soxA)
SERT: TAN611_2783(prnC)
DYE: EO087_02530
PAG: PLES_25781 PLES_25851(pltD)
PAF: PAM18_2383(pltM) PAM18_2389(pltD)
PAEL: T223_13175 T223_13210
PFL: PFL_2784(pltM) PFL_2790(pltD) PFL_3606(prnC)
PPRC: PFLCHA0_c28420(pltM) PFLCHA0_c28480(pltD) PFLCHA0_c36470(prnC)
PPRO: PPC_2829(pltM) PPC_2835(pltD) PPC_3745(prnC)
PCH: EY04_18025
PCZ: PCL1606_28090
PCP: JM49_15080
PSEM: TO66_16625
PPII: QL104_13925 QL104_13955 QL104_19155 QL104_23510
PCAA: V6L78_22535
CJA: CJA_2999(halA)
MMT: Metme_1103
HCH: HCH_02500
HAHE: ENC22_11335
HAHH: O5O45_06390
ZPL: ZBT109_0564
BMA: BMAA1201(prnC)
BMV: BMASAVP1_0165(prnC)
BML: BMA10229_0442(prnC)
BMN: BMA10247_A1135(prnC)
BMAZ: BM44_4276
BMAL: DM55_4314
BMAE: DM78_3855
BMAQ: DM76_3266
BMAI: DM57_4424
BMAF: DM51_4862
BMAB: BM45_3400
BPS: BPSS1010
BPM: BURPS1710b_A0003(prnC) BURPS1710b_A1117(prnC)
BPL: BURPS1106A_A1397(halB) BURPS1106A_A2735
BPD: BURPS668_A1482 BURPS668_A2887
BPR: GBP346_B0728 GBP346_B2174
BPSE: BDL_4306 BDL_5424
BPSM: BBQ_4119 BBQ_5121
BPSU: BBN_4474 BBN_5477
BPSD: BBX_4591 BBX_6149
BPZ: BP1026B_II1107
BPQ: BPC006_II1407 BPC006_II2698
BPK: BBK_4753 BBK_6130
BPSH: DR55_4322 DR55_5720
BPSA: BBU_3997 BBU_5014
BPSO: X996_4250 X996_5524
BUT: X994_5082 X994_6109
BTZ: BTL_5468
BTHA: DR62_5268
BOK: DM82_2185 DM82_6304
BOC: BG90_2527
BUU: WS70_23845
BSAV: WS86_22610
BUR: Bcep18194_C6708
BCM: Bcenmc03_6985
BAM: Bamb_4728
BAC: BamMC406_5266
BCEP: APZ15_34530
BPYR: ABD05_31830
BCON: NL30_32790
BUB: BW23_5780
BSEM: WJ12_18240
BMEC: WJ16_17190
BARI: NLX30_39210
BSER: R0290_29225
BGD: bgla_2g02310
BURK: DM992_38825
BURM: GJG85_34560
BURF: Bsp3421_002342
BUV: FPJ27_11850
MBD: MEBOL_008076
PBRU: LZC95_06800
PALJ: LZC94_20375
SAMY: DB32_006387
NANN: O0S08_14700
RBA: RB1653(prnC)
RHOI: PSR62_20040
PIR: VN12_12750
RUL: UC8_31640
ROL: CA51_40950
RUV: EC9_43150
AHEL: Q31a_04260
RLC: K227x_49800
SMAM: Mal15_37510
SNEP: Enr13x_29450
SMAE: TBK1r_19870
PLS: VT03_06115
FMR: Fuma_06477
GMR: GmarT_34080
GIM: F1728_29395
GPN: Pan110_32210
GFM: Enr17x_33880
GAZ: Pan241w_32920
GAW: V144x_26700
GAX: Pan161_35060
MRI: Mal4_16350
ACAF: CA12_04690
PVAR: SH412_004361
CCOP: Mal65_29530
SCHL: QJS52_15815
SSPE: ACGY09_13885
GES: VT84_18145
GMS: SOIL9_20870
LRS: PX52LOC_00381
FTJ: FTUN_8502
ULI: ETAA1_02960
UHL: UABHE_004247 UABHE_004920
ABAS: ACPOL_5987
EPL: P4G45_06590
ADIN: H7849_12765
ABAC: LuPra_02345
GBA: J421_4025
THEO: IMW88_05220
MGIN: FRZ54_14670
RMR: Rmar_2072
RMG: Rhom172_0794
RLO: GQ464_016890
RBAR: AWN76_015905
CPRV: CYPRO_2187
PIH: UB51_18965
PCHI: PC41400_07730
MAB: MAB_2284
MMV: MYCMA_09365
MABB: MASS_2208
MABL: MMASJCM_2233
MIZ: BAB75_12200
MSTE: MSTE_02497
NFR: ERS450000_00759
NCY: NOCYR_5153
NBR: O3I_018870
NNO: NONO_c51090(prnC)
NTP: CRH09_09475 CRH09_09510
NOZ: DMB37_02795
NOD: FOH10_15045 FOH10_23420
NWL: NWFMUON74_24270(prnC)
NVU: V7968_26565
RRZ: CS378_19305
RHOY: QNA09_01210
SCO: SCO1275(2SCG18.22)
SFI: SFUL_3996
SSX: SACTE_6340
SRC: M271_09845
SVE: SVEN_0488
SDV: BN159_0503 BN159_0504 BN159_2478 BN159_2479 BN159_2480 BN159_2482 BN159_2483(halB) BN159_2484
SLV: SLIV_31415
SGU: SGLAU_28900 SGLAU_28930
SAMB: SAM23877_1355(prnC)
SCZ: ABE83_16275
SPAV: Spa2297_04645
SGS: AVL59_19215
SCAD: DN051_32680
SPHW: NFX46_19280
STRD: NI25_18925 NI25_32825
SNZ: DC008_13265
SVQ: N6Q81_05840
SGD: ELQ87_03590
SVR: CP971_23950
SFY: GFH48_20345
SCAV: CVT27_18180
SPAC: B1H29_04740 B1H29_31235
SCHA: CP983_36975
SCHF: IPT68_15460
SFEU: IM697_16645
SMOB: J7W19_30865
SCIR: STRCI_006110 STRCI_006112 STRCI_006113 STRCI_006114 STRCI_007874 STRCI_007875
STUD: STRTU_005058
KBU: Q4V64_07440
SARM: DVA86_03325 DVA86_18260 DVA86_18265 DVA86_18280
SPRF: K4G22_30710 K4G22_30780
SMIB: SMIR_18250
STRL: HEP84_26850
STSD: HEP87_27820
SRPZ: HEP85_21235
STRV: EZV63_04645
SMOD: POD33_29405
STBR: ABDE16_32230
SSOC: ABZO31_28195
STYI: C9F11_44430
SFLU: ACKTSN_12250 ACKTSN_40245
SSTO: ACE1OC_42610
STCZ: JHY03_37920
SCT: SCAT_5600
SCY: SCATT_55980
KAU: B6264_18525
KCH: O1G21_22165
STRI: C7M71_024630
ABRY: NYE86_15915 NYE86_16955
ACTW: F7P10_30225
SPIQ: OHA34_06380
ACTQ: OG417_42950
FRA: Francci3_0437
FAL: FRAAL0926
FRE: Franean1_6189
SEN: SACE_4927
AORI: SD37_34235
SSYI: EKG83_28905
ASE: ACPL_6150
ACTS: ACWT_6018
AIH: Aiant_03700
ACTR: Asp14428_76420
ACTL: L3i22_052610
AOU: ACTOB_004306 ACTOB_005290
PSUU: Psuf_005050(prnC) Psuf_005170
CATI: CS0771_15270(prnC) CS0771_15280 CS0771_15290
DVC: Dvina_26625
DAUR: Daura_27000 Daura_37810
DMAT: Dmats_22070
CAI: Caci_3552 Caci_6353
AMR: AM1_6083
CTHE: Chro_3657
CAP: CLDAP_18670
CCZ: CCALI_00884
HLT: I7X12_12635
HALE: G3A49_13210
HGI: ABY42_10695
HLR: HALLA_01030
AG: AAB97506(prnC) AEI98659(ctcP) BAA07389(cts4)
 » show all
Reference
PMID:7612997 (cts4)
  Authors
Dairi T, Nakano T, Aisaka K, Katsumata R, Hasegawa M.
  Title
Cloning and nucleotide sequence of the gene responsible for chlorination of tetracycline.
  Journal
Biosci Biotechnol Biochem 59:1099-106 (1995)
DOI:10.1271/bbb.59.1099
  Sequence
[ag:BAA07389]
Reference
PMID:9172332 (prnC)
  Authors
Hammer PE, Hill DS, Lam ST, Van Pee KH, Ligon JM
  Title
Four genes from Pseudomonas fluorescens that encode the biosynthesis of pyrrolnitrin.
  Journal
Appl Environ Microbiol 63:2147-54 (1997)
DOI:10.1128/AEM.63.6.2147-2154.1997
  Sequence
[ag:AAB97506]
Reference
PMID:23800859 (ctcP)
  Authors
Zhu T, Cheng X, Liu Y, Deng Z, You D
  Title
Deciphering and engineering of the final step halogenase for improved chlortetracycline biosynthesis in industrial Streptomyces aureofaciens.
  Journal
Metab Eng 19:69-78 (2013)
DOI:10.1016/j.ymben.2013.06.003
  Sequence
[ag:AEI98659]
Reference
PMID:16895332
  Authors
Vaillancourt FH, Yeh E, Vosburg DA, Garneau-Tsodikova S, Walsh CT
  Title
Nature's inventory of halogenation catalysts: oxidative strategies predominate.
  Journal
Chem Rev 106:3364-78 (2006)
DOI:10.1021/cr050313i
LinkDB

KEGG   ORTHOLOGY: K21297
Entry
K21297                      KO                                     
Symbol
ctcQ
Name
flavin reductase (NADH) [EC:1.5.1.36]
Pathway
map00253  Tetracycline biosynthesis
map01057  Biosynthesis of type II polyketide products
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00823  Chlortetracycline biosynthesis, pretetramide => chlortetracycline
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K21297  ctcQ; flavin reductase (NADH)
   00253 Tetracycline biosynthesis
    K21297  ctcQ; flavin reductase (NADH)
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K21297  ctcQ; flavin reductase (NADH)
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.5  Acting on the CH-NH group of donors
   1.5.1  With NAD+ or NADP+ as acceptor
    1.5.1.36  flavin reductase (NADH)
     K21297  ctcQ; flavin reductase (NADH)
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide tailoring proteins
  Others
   K21297  ctcQ; flavin reductase (NADH)
Other DBs
COG: COG1853
GO: 0036382
Genes
AMYL: QBD29_13685
MMI: MMAR_3634
MMAE: MMARE11_35420
MLI: MULP_03882
MPSE: MPSD_37010
MSHO: MSHO_33260
MMAG: MMAD_31880
NSR: NS506_02368
RKO: JWS14_38875
RHOJ: JVH1_22090
RHJS: OYT95_23350
RHWB: FND50_32390
GJI: H1R19_00440
SANL: KZO11_19400 KZO11_19470
SGRF: SGFS_028000
SHY: SHJG_8629
SHO: SHJGH_8389
SRC: M271_09870
SSOI: I1A49_36865
SVE: SVEN_0489 SVEN_6227
SDV: BN159_2477(pyr9)
SGU: SGLAU_04550
STRE: GZL_02226 GZL_02917
SLD: T261_7912
SRW: TUE45_00621(rbmH_1) TUE45_06418(rbmH_2)
SGS: AVL59_00130
SCAD: DN051_13760
SPHW: NFX46_19275
SNR: SNOUR_09915 SNOUR_12600
SGD: ELQ87_03565
SNQ: CP978_29025
SKA: CP970_02915
SRK: FGW37_26495
SVR: CP971_23945
SPAD: DVK44_09675
SBY: H7H31_23750
SRIM: CP984_09615
SSUB: CP968_16675 CP968_32175
SPHV: F9278_28180
SPAC: B1H29_04715
SCHA: CP983_18380
SNF: JYK04_01553(rbmH)
SPLA: CP981_13855 CP981_21750
SHUN: DWB77_02860(rbmH)
SLIA: HA039_20555
SDD: D9753_04010
SMAO: CAG99_27175
SAOV: G3H79_23740 G3H79_33555
SYAN: NRK68_04400
STUB: MMF93_00210
SCIR: STRCI_006115
SYUN: MOV08_09875 MOV08_12410
SCYN: N8I84_12740
SOV: QZH56_05190 QZH56_16715 QZH56_21230
SHAU: K9S39_20815
SCOA: QU709_17560
STTN: BSL84_04145
STRZ: OYE22_22945
KBU: Q4V64_34035
SARM: DVA86_03350
SPRF: K4G22_30755
STPB: QR97_23405
SRPA: HEP86_14930 HEP86_22875
SMIB: SMIR_00260 SMIR_37550
STRW: QHG49_17495
STRL: HEP84_07605 HEP84_44360 HEP84_54010
STSD: HEP87_45490
SCHE: QRN89_03810
SGLY: P8A20_25385
SMOD: POD33_15690 POD33_23090
STBR: ABDE16_07610
STYI: C9F11_35415(rbmH1) C9F11_44425(rbmH2) C9F11_46025(hsaB4)
SEXP: SLV14_003492
SFLU: ACKTSN_40270
STGO: KBP30_28110 KBP30_37900
STID: G7Z12_16225
STSG: B6R96_31205
STHU: M1P56_22890
KAU: B6264_18520
KCH: O1G21_36975
STRH: GXP74_20075 GXP74_21290
RHAL: LQF10_02755
NFE: HUT17_04640
ACTQ: OG417_42925
SEN: SACE_4928
AOI: AORI_5335
AORI: SD37_34215
ARHD: VSH64_07725
AMYZ: HUW46_09457(rbmH)
ACAB: QRX50_38600
AMYD: K1T34_37995
PSEQ: AD006_32420
SESP: BN6_32530 BN6_35870
KAL: KALB_2183 KALB_5899 KALB_6285
KUT: JJ691_19090 JJ691_20620
KBR: Q4V65_13520 Q4V65_51030
LXR: ACRSBM_32730
AHM: TL08_07685
ACAU: ACOJVX_12005
UME: RM788_41075
MCRA: ID554_06685
VER: HUT12_06275
MFEU: H1D33_25005
VET: ACQVFL_05115 ACQVFL_17400
ASE: ACPL_6145(ntaB)
ACTS: ACWT_6013
AOU: ACTOB_004311
DVC: Dvina_05880 Dvina_38690
DAUR: Daura_37835
DMAT: Dmats_05360 Dmats_33650
NHY: JQS43_08500
AG: AEI98660(ctcQ)
 » show all
Reference
PMID:23800859
  Authors
Zhu T, Cheng X, Liu Y, Deng Z, You D
  Title
Deciphering and engineering of the final step halogenase for improved chlortetracycline biosynthesis in industrial Streptomyces aureofaciens.
  Journal
Metab Eng 19:69-78 (2013)
DOI:10.1016/j.ymben.2013.06.003
  Sequence
[ag:AEI98660]
LinkDB

KEGG   ENZYME: 1.14.19.49
Entry
EC 1.14.19.49               Enzyme                                 
Name
tetracycline 7-halogenase;
ctcP (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
tetracycline:FADH2 oxidoreductase (7-halogenating)
Reaction(IUBMB)
tetracycline + FADH2 + chloride + O2 + H+ = 7-chlorotetracycline + FAD + 2 H2O [RN:R11478]
Reaction(KEGG)
R11478
Substrate
tetracycline [CPD:C06570];
FADH2 [CPD:C01352];
chloride [CPD:C00698];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
7-chlorotetracycline [CPD:C06571];
FAD [CPD:C00016];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Streptomyces aureofaciens, is a member of the flavin-dependent halogenase family. The enzyme forms a lysine chloramine intermediate on an internal lysine residue before transferring the chlorine to the substrate. It is stereo-selective for the 4S (natural) isomer of tetracycline. FADH2 is provided by a dedicated EC 1.5.1.36, flavin reductase (NADH).
History
EC 1.14.19.49 created 2016
Pathway
ec00253  Tetracycline biosynthesis
ec01057  Biosynthesis of type II polyketide products
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K14257  tetracycline 7-halogenase / FADH2 O2-dependent halogenase
Genes
ECLN: ECNIH4_08285
ENO: ECENHK_14345
ECHG: FY206_15895
END: A4308_19035
ENZ: G0034_14940
SRR: SerAS9_4266
SRL: SOD_c39980(prnC)
SRY: M621_21735
SPLY: Q5A_021590(soxA)
SRS: SerAS12_4267
SRA: SerAS13_4267
SMAF: D781_2599
SRZ: AXX16_2518
SERM: CLM71_13480
SRHZ: FO014_20185
SGRI: SGBXF1_00335(soxA)
SERT: TAN611_2783(prnC)
DYE: EO087_02530
PAG: PLES_25781 PLES_25851(pltD)
PAF: PAM18_2383(pltM) PAM18_2389(pltD)
PAEL: T223_13175 T223_13210
PFL: PFL_2784(pltM) PFL_2790(pltD) PFL_3606(prnC)
PPRC: PFLCHA0_c28420(pltM) PFLCHA0_c28480(pltD) PFLCHA0_c36470(prnC)
PPRO: PPC_2829(pltM) PPC_2835(pltD) PPC_3745(prnC)
PCH: EY04_18025
PCZ: PCL1606_28090
PCP: JM49_15080
PSEM: TO66_16625
PPII: QL104_13925 QL104_13955 QL104_19155 QL104_23510
PCAA: V6L78_22535
CJA: CJA_2999(halA)
MMT: Metme_1103
HCH: HCH_02500
HAHE: ENC22_11335
HAHH: O5O45_06390
ZPL: ZBT109_0564
BMA: BMAA1201(prnC)
BMV: BMASAVP1_0165(prnC)
BML: BMA10229_0442(prnC)
BMN: BMA10247_A1135(prnC)
BMAZ: BM44_4276
BMAL: DM55_4314
BMAE: DM78_3855
BMAQ: DM76_3266
BMAI: DM57_4424
BMAF: DM51_4862
BMAB: BM45_3400
BPS: BPSS1010
BPM: BURPS1710b_A0003(prnC) BURPS1710b_A1117(prnC)
BPL: BURPS1106A_A1397(halB) BURPS1106A_A2735
BPD: BURPS668_A1482 BURPS668_A2887
BPR: GBP346_B0728 GBP346_B2174
BPSE: BDL_4306 BDL_5424
BPSM: BBQ_4119 BBQ_5121
BPSU: BBN_4474 BBN_5477
BPSD: BBX_4591 BBX_6149
BPZ: BP1026B_II1107
BPQ: BPC006_II1407 BPC006_II2698
BPK: BBK_4753 BBK_6130
BPSH: DR55_4322 DR55_5720
BPSA: BBU_3997 BBU_5014
BPSO: X996_4250 X996_5524
BUT: X994_5082 X994_6109
BTZ: BTL_5468
BTHA: DR62_5268
BOK: DM82_2185 DM82_6304
BOC: BG90_2527
BUU: WS70_23845
BSAV: WS86_22610
BUR: Bcep18194_C6708
BCM: Bcenmc03_6985
BAM: Bamb_4728
BAC: BamMC406_5266
BCEP: APZ15_34530
BPYR: ABD05_31830
BCON: NL30_32790
BUB: BW23_5780
BSEM: WJ12_18240
BMEC: WJ16_17190
BARI: NLX30_39210
BSER: R0290_29225
BGD: bgla_2g02310
BURK: DM992_38825
BURM: GJG85_34560
BURF: Bsp3421_002342
BUV: FPJ27_11850
MBD: MEBOL_008076
PBRU: LZC95_06800
PALJ: LZC94_20375
SAMY: DB32_006387
NANN: O0S08_14700
RBA: RB1653(prnC)
RHOI: PSR62_20040
PIR: VN12_12750
RUL: UC8_31640
ROL: CA51_40950
RUV: EC9_43150
AHEL: Q31a_04260
RLC: K227x_49800
SMAM: Mal15_37510
SNEP: Enr13x_29450
SMAE: TBK1r_19870
PLS: VT03_06115
FMR: Fuma_06477
GMR: GmarT_34080
GIM: F1728_29395
GPN: Pan110_32210
GFM: Enr17x_33880
GAZ: Pan241w_32920
GAW: V144x_26700
GAX: Pan161_35060
MRI: Mal4_16350
ACAF: CA12_04690
PVAR: SH412_004361
CCOP: Mal65_29530
SCHL: QJS52_15815
SSPE: ACGY09_13885
GES: VT84_18145
GMS: SOIL9_20870
LRS: PX52LOC_00381
FTJ: FTUN_8502
ULI: ETAA1_02960
UHL: UABHE_004247 UABHE_004920
ABAS: ACPOL_5987
EPL: P4G45_06590
ADIN: H7849_12765
ABAC: LuPra_02345
GBA: J421_4025
THEO: IMW88_05220
MGIN: FRZ54_14670
RMR: Rmar_2072
RMG: Rhom172_0794
RLO: GQ464_016890
RBAR: AWN76_015905
CPRV: CYPRO_2187
PIH: UB51_18965
PCHI: PC41400_07730
MAB: MAB_2284
MMV: MYCMA_09365
MABB: MASS_2208
MABL: MMASJCM_2233
MIZ: BAB75_12200
MSTE: MSTE_02497
NFR: ERS450000_00759
NCY: NOCYR_5153
NBR: O3I_018870
NNO: NONO_c51090(prnC)
NTP: CRH09_09475 CRH09_09510
NOZ: DMB37_02795
NOD: FOH10_15045 FOH10_23420
NWL: NWFMUON74_24270(prnC)
NVU: V7968_26565
RRZ: CS378_19305
RHOY: QNA09_01210
SCO: SCO1275(2SCG18.22)
SFI: SFUL_3996
SSX: SACTE_6340
SRC: M271_09845
SVE: SVEN_0488
SDV: BN159_0503 BN159_0504 BN159_2478 BN159_2479 BN159_2480 BN159_2482 BN159_2483(halB) BN159_2484
SLV: SLIV_31415
SGU: SGLAU_28900 SGLAU_28930
SAMB: SAM23877_1355(prnC)
SCZ: ABE83_16275
SPAV: Spa2297_04645
SGS: AVL59_19215
SCAD: DN051_32680
SPHW: NFX46_19280
STRD: NI25_18925 NI25_32825
SNZ: DC008_13265
SVQ: N6Q81_05840
SGD: ELQ87_03590
SVR: CP971_23950
SFY: GFH48_20345
SCAV: CVT27_18180
SPAC: B1H29_04740 B1H29_31235
SCHA: CP983_36975
SCHF: IPT68_15460
SFEU: IM697_16645
SMOB: J7W19_30865
SCIR: STRCI_006110 STRCI_006112 STRCI_006113 STRCI_006114 STRCI_007874 STRCI_007875
STUD: STRTU_005058
KBU: Q4V64_07440
SARM: DVA86_03325 DVA86_18260 DVA86_18265 DVA86_18280
SPRF: K4G22_30710 K4G22_30780
SMIB: SMIR_18250
STRL: HEP84_26850
STSD: HEP87_27820
SRPZ: HEP85_21235
STRV: EZV63_04645
SMOD: POD33_29405
STBR: ABDE16_32230
SSOC: ABZO31_28195
STYI: C9F11_44430
SFLU: ACKTSN_12250 ACKTSN_40245
SSTO: ACE1OC_42610
STCZ: JHY03_37920
SCT: SCAT_5600
SCY: SCATT_55980
KAU: B6264_18525
KCH: O1G21_22165
STRI: C7M71_024630
ABRY: NYE86_15915 NYE86_16955
ACTW: F7P10_30225
SPIQ: OHA34_06380
ACTQ: OG417_42950
FRA: Francci3_0437
FAL: FRAAL0926
FRE: Franean1_6189
SEN: SACE_4927
AORI: SD37_34235
SSYI: EKG83_28905
ASE: ACPL_6150
ACTS: ACWT_6018
AIH: Aiant_03700
ACTR: Asp14428_76420
ACTL: L3i22_052610
AOU: ACTOB_004306 ACTOB_005290
PSUU: Psuf_005050(prnC) Psuf_005170
CATI: CS0771_15270(prnC) CS0771_15280 CS0771_15290
DVC: Dvina_26625
DAUR: Daura_27000 Daura_37810
DMAT: Dmats_22070
CAI: Caci_3552 Caci_6353
AMR: AM1_6083
CTHE: Chro_3657
CAP: CLDAP_18670
CCZ: CCALI_00884
HLT: I7X12_12635
HALE: G3A49_13210
HGI: ABY42_10695
HLR: HALLA_01030
 » show all
Reference
1  [PMID:7612997]
  Authors
Dairi T, Nakano T, Aisaka K, Katsumata R, Hasegawa M.
  Title
Cloning and nucleotide sequence of the gene responsible for chlorination of tetracycline.
  Journal
Biosci Biotechnol Biochem 59:1099-106 (1995)
DOI:10.1271/bbb.59.1099
  Sequence
[ag:BAA07389]
Reference
2  [PMID:23800859]
  Authors
Zhu T, Cheng X, Liu Y, Deng Z, You D
  Title
Deciphering and engineering of the final step halogenase for improved chlortetracycline biosynthesis in industrial Streptomyces aureofaciens.
  Journal
Metab Eng 19:69-78 (2013)
DOI:10.1016/j.ymben.2013.06.003
  Sequence
[ag:AEI98659 AEI98660]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.49
IUBMB Enzyme Nomenclature: 1.14.19.49
ExPASy - ENZYME nomenclature database: 1.14.19.49
BRENDA, the Enzyme Database: 1.14.19.49
LinkDB

KEGG   REACTION: R11478
Entry
R11478                      Reaction                               
Name
tetracycline:FADH2 oxidoreductase (7-halogenating)
Definition
Tetracycline + FADH2 + Cl- + Oxygen + H+ <=> Chlortetracycline + FAD + 2 H2O
Equation
C06570 + C01352 + C00698 + C00007 + C00080 <=> C06571 + C00016 + 2 C00001
Reaction class
RC00949  C06570_C06571
Enzyme
1.14.19.49
Pathway
rn00253  Tetracycline biosynthesis
rn01057  Biosynthesis of type II polyketide products
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00823  Chlortetracycline biosynthesis, pretetramide => chlortetracycline
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.49
     R11478  Tetracycline + FADH2 + Cl- + Oxygen + H+ <=> Chlortetracycline + FAD + 2 H2O
Orthology
K14257  tetracycline 7-halogenase / FADH2 O2-dependent halogenase [EC:1.14.19.49 1.14.19.-]
Other DBs
RHEA: 50715
LinkDB

DBGET integrated database retrieval system