KEGG   ORTHOLOGY: K14451
Entry
K14451                      KO                                     
Symbol
mcl2
Name
(3S)-malyl-CoA thioesterase [EC:3.1.2.30]
Pathway
map00630  Glyoxylate and dicarboxylate metabolism
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01200  Carbon metabolism
Module
M00373  Ethylmalonyl pathway
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00630 Glyoxylate and dicarboxylate metabolism
    K14451  mcl2; (3S)-malyl-CoA thioesterase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.1  Acting on ester bonds
   3.1.2  Thioester hydrolases
    3.1.2.30  (3S)-malyl-CoA thioesterase
     K14451  mcl2; (3S)-malyl-CoA thioesterase
Other DBs
RN: R10612
COG: COG2301
Genes
BOJ: CBF45_15340
KGY: EHF36_15360
CPAF: C6V80_02765
BRD: JL11_15545
BRG: A4249_01015
BVC: CEP68_03750
BREV: E7T10_13660
BVY: NCTC9239_00695(mcl2)
AEX: Astex_0375
SIL: SPO0352(citE)
RUT: FIU92_01320(mcl1)
JAN: Jann_0816
RDE: RD1_1620(citE)
DSH: Dshi_2874
PGD: Gal_03134
OTM: OSB_04760(mcl2)
LAQU: R2C4_13635
CID: P73_3868
MALG: MALG_02745
SINL: DSM14862_02486(mcl2)
SPSE: SULPSESMR1_00394(mcl2)
RMM: ROSMUCSMR3_02505(mcl2)
RID: RIdsm_04538(mcl2)
ROH: FIU89_19180(mcl1)
AHT: ANTHELSMS3_02127(mcl2)
MARU: FIU81_04115(mcl1)
ROT: FIV09_01380(mcl1)
MALU: KU6B_41840(mcl2)
RSP: RSP_0970
RCP: RCAP_rcc00727(citE1)
PDE: Pden_0563
PMAU: CP157_01939(mcl2)
PTP: RCA23_c14020(citE)
RSU: NHU_00724(citE)
RHC: RGUI_0622
LVS: LOKVESSMR4R_02803(mcl2)
PAMO: BAR1_14395
PALW: PSAL_019030(mcl2)
SPHP: LH20_16080
SMAZ: LH19_01485
SGI: SGRAN_0950(mcl2)
SPHU: SPPYR_0495(mcl)
SPHI: TS85_09155
SSAN: NX02_12375
SBIN: SBA_ch1_14890(citE)
SJP: SJA_C1-26890(citE)
SINB: SIDU_01380
SSY: SLG_14830
SPMI: K663_04105
SPHB: EP837_00844(citE)
SPHR: BSY17_744
SPHT: K426_06735
DGO: DGo_CA0363(citE)
DFC: DFI_10115
BLQ: L21SP5_01621(mcl2)
 » show all
Reference
  Authors
Alber BE
  Title
Biotechnological potential of the ethylmalonyl-CoA pathway.
  Journal
Appl Microbiol Biotechnol 89:17-25 (2011)
DOI:10.1007/s00253-010-2873-z
Reference
  Authors
Erb TJ, Frerichs-Revermann L, Fuchs G, Alber BE
  Title
The apparent malate synthase activity of Rhodobacter sphaeroides is due to two paralogous enzymes, (3S)-Malyl-coenzyme A (CoA)/{beta}-methylmalyl-CoA lyase and (3S)- Malyl-CoA thioesterase.
  Journal
J Bacteriol 192:1249-58 (2010)
DOI:10.1128/JB.01267-09
  Sequence
[rsp:RSP_0970]
LinkDB

KEGG   ORTHOLOGY: K19282
Entry
K19282                      KO                                     
Symbol
aceB
Name
bifunctional (S)-malyl-CoA lyase/thioesterase [EC:4.1.3.24 3.1.2.30]
Pathway
map00630  Glyoxylate and dicarboxylate metabolism
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01200  Carbon metabolism
Module
M00012  Glyoxylate cycle
M00740  Methylaspartate cycle
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00630 Glyoxylate and dicarboxylate metabolism
    K19282  aceB; bifunctional (S)-malyl-CoA lyase/thioesterase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.1  Acting on ester bonds
   3.1.2  Thioester hydrolases
    3.1.2.30  (3S)-malyl-CoA thioesterase
     K19282  aceB; bifunctional (S)-malyl-CoA lyase/thioesterase
 4. Lyases
  4.1  Carbon-carbon lyases
   4.1.3  Oxo-acid-lyases
    4.1.3.24  malyl-CoA lyase
     K19282  aceB; bifunctional (S)-malyl-CoA lyase/thioesterase
Other DBs
RN: R00473 R10612
GO: 0050083
Genes
HDL: HALDL1_15310
HHB: Hhub_3380(aceB)
HNO: LT974_13710
HLU: LT972_01030
HSU: HLASF_2007(aceB)
HSF: HLASA_1993(aceB)
SALR: FQU85_05715
HAAD: MW046_18245
HALX: M0R89_21245
SAIM: K0C01_12450
HMA: rrnAC1965(aceB)
HHI: HAH_2476(aceB)
NPH: NP_4430A(aceB)
NMO: Nmlp_2357(aceB)
HWA: HQ_1720A(aceB)
HWC: Hqrw_1842(aceB)
HVO: HVO_1983(aceB1) HVO_B0200(aceB2)
HME: HFX_2078(aceB)
HDF: AArcSl_1031(aceB)
HTU: Htur_4695
NMG: Nmag_3363(aceB)
 » show all
Reference
  Authors
Khomyakova M, Bukmez O, Thomas LK, Erb TJ, Berg IA
  Title
A methylaspartate cycle in haloarchaea.
  Journal
Science 331:334-7 (2011)
DOI:10.1126/science.1196544
  Sequence
[hma:rrnAC1965]
LinkDB

KEGG   ENZYME: 3.1.2.30
Entry
EC 3.1.2.30                 Enzyme                                 
Name
(3S)-malyl-CoA thioesterase;
mcl2 (gene name)
Class
Hydrolases;
Acting on ester bonds;
Thioester hydrolases
Sysname
(S)-malyl-CoA hydrolase
Reaction(IUBMB)
(S)-malyl-CoA + H2O = (S)-malate + CoA [RN:R10612]
Reaction(KEGG)
R10612
Substrate
(S)-malyl-CoA [CPD:C04348];
H2O [CPD:C00001]
Product
(S)-malate [CPD:C00149];
CoA [CPD:C00010]
Comment
Stimulated by Mg2+ or Mn2+. The enzyme has no activity with (2R,3S)-2-methylmalyl-CoA (cf. EC 4.1.3.24, malyl-CoA lyase) or other CoA esters.
History
EC 3.1.2.30 created 2014
Pathway
ec00630  Glyoxylate and dicarboxylate metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K14451  (3S)-malyl-CoA thioesterase
K19282  bifunctional (S)-malyl-CoA lyase/thioesterase
Genes
BOJCBF45_15340
KGYEHF36_15360
CPAFC6V80_02765
BRDJL11_15545
BRGA4249_01015
BVCCEP68_03750
BREVE7T10_13660
BVYNCTC9239_00695(mcl2)
AEXAstex_0375
SILSPO0352(citE)
SITTM1040_3520
RUAD1823_12170
RUTFIU92_01320(mcl1)
RCONK3740_01470
JANJann_0816
RDERD1_1620(citE)
RLIRLO149_c030880
RPONG3256_15365
DSHDshi_2874
PGAPGA1_c03680
PGLPGA2_c03240
PGDGal_03134
PHPPhaeoP97_02988
PPICPhaeoP14_00285
PHQD1820_14695
OATOAN307_c41520
OAROA238_c04250
OTMOSB_04760(mcl2)
OCTFTO60_02985
LMDMETH_03315
LEJETW24_04120
LAQUR2C4_13635
LCAEK3721_03435
REDroselon_01727
CIDP73_3868
CMARIMCC12053_3020
CEHCEW89_10100
MALGMALG_02745
CONTQ29_02440
YPACCEW88_11165
YANAYJ57_04550
TPROGa0080559_TMP1136
PABYGa0080574_TMP3142
SUAMBOO69_13680
SULZC1J03_18190
SULIC1J05_18110
SULDB5M07_04980
SPOTG6548_04145
SMEDJNX03_18050
SINLDSM14862_02486(mcl2)
SPSESULPSESMR1_00394(mcl2)
TOMBWR18_01025
RMMROSMUCSMR3_02505(mcl2)
ROKRAK1035_0677
RIDRIdsm_04538(mcl2)
ROMEI983_02900
ROHFIU89_19180(mcl1)
AHTANTHELSMS3_02127(mcl2)
SAGUCDO87_04390
THAACFI11_04355
OCDFHY55_05855
MARUFIU81_04115(mcl1)
ROTFIV09_01380(mcl1)
RMHLVO79_01055
MALUKU6B_41840(mcl2)
TGLHFZ77_08415
HATRC74_09365
PALXGQA70_04790
LSALKBK07_01415
RSPRSP_0970
RSHRsph17029_2630
RSQRsph17025_0082
RSKRSKD131_2362
CAZTLV780_00415
RCPRCAP_rcc00727(citE1)
RHPLPB142_03415
RBLB6K69_07625
PDEPden_0563
PAMIJCM7686_2574
PYEA6J80_13300
PCONB0A89_09550
PZHCX676_05535
PAROCUV01_17720
PAMNJCM7685_2545
PARSDRW48_09375
PARREOJ32_08540
PAAKFIU66_10030
PKDF8A10_02165
PPANESD82_12320
PLIAE4191_08475
PSANHGN31_04095
PMAUCP157_01939(mcl2)
PMEHJWJ88_01995
PTPRCA23_c14020(citE)
RSUNHU_00724(citE)
RHMB5V46_04475
RHCRGUI_0622
RMBK529_020740
DAAAKL17_3637
DONBSK21_11440
RBZB9057_02175
THWBMG03_06670
TECAKL02_005525
LVSLOKVESSMR4R_02803(mcl2)
RBGBG454_13655
GEHHYN69_13115
GFUKM031_13965
TAWEI545_06975
SALOEF888_18565
SEDIEBB79_02250
HMLHmaOT1_03960
BOOE2K80_01990
PSEBEOK75_09400
LITFPZ52_01350
PPRUFDP22_03640
PAEDG5B38_15490
MONG8E03_14605
PAMOBAR1_14395
PSHQF3W81_20275
POZI0K15_13770
PALWPSAL_019030(mcl2)
PPAFI8N54_16895
CACTHZ995_03660
THASC6Y53_10240
HDHG5B40_16110
CMAGCBW24_13080
FAPGR316_00365
GCEKYE46_04235
NSMJO391_19965
ACROK3J57_02795
SINRO5O51_12655
FAQG5B39_05115
AMAQGO499_14350
HYTHXX25_10655
SPHKSKP52_03440
SPHPLH20_16080
SMAGAN936_01440
SMAZLH19_01485
STERAOA14_02930
SGISGRAN_0950(mcl2)
SPHQBWQ93_11140
SPHOC3E99_01130
SPHXE5675_17240
SPHUSPPYR_0495(mcl)
SPHITS85_09155
SSANNX02_12375
SPLMBXU08_04065
SPHCCVN68_04030
SLANGV829_09535
SPAIFPZ24_11710
SARIH5J25_02820
SSUAFPZ54_04690
SBINSBA_ch1_14890(citE)
SJPSJA_C1-26890(citE)
SINBSIDU_01380
SCHSphch_1616
SSYSLG_14830
SYBTZ53_00395
SBDATN00_05850
SPMIK663_04105
SPHBEP837_00844(citE)
SPHRBSY17_744
SPHTK426_06735
SHYDCJD35_07870
SYAA6768_15560
SCLOSCLO_1015050
SPYGYGS_C1P0058
SUFLFIL70_12030
SBARH5V43_09595
SAMISAMIE_1022050
SPZRG5C33_12750
PALGHFP57_06190
SPHSETR14_23585
RPYY013_11815
RBYCEJ39_22715
RHQIM25_03505
GTABCM27_21690
BSOLFSW04_20430
DDRDeide_20020
DMRDeima_2864
DPTDeipr_2520
DGODGo_CA0363(citE)
DSWQR90_15895
DCHSY84_10370
DABAUC44_01395
DPUSU48_13230
DWUDVJ83_08830
DFCDFI_10115
DEINDAAJ005_03145
BLQL21SP5_01621(mcl2)
HDLHALDL1_15310
HHBHhub_3380(aceB)
HNOLT974_13710
HLULT972_01030
HSUHLASF_2007(aceB)
HSFHLASA_1993(aceB)
SALRFQU85_05715
HAADMW046_18245
HALXM0R89_21245
SAIMK0C01_12450
HMArrnAC1965(aceB)
HHIHAH_2476(aceB)
HHNHISP_12605
HABSG26_04910
HTABVU17_12045
HSINKDQ40_00995
NPHNP_4430A(aceB)
NMONmlp_2357(aceB)
NHOHWV23_13655
NSALHWV07_07395
HALIBV210_07645
HDOMUK72_12480
SSAIN0B31_16615
HWAHQ_1720A(aceB)
HWCHqrw_1842(aceB)
HVOHVO_1983(aceB1) HVO_B0200(aceB2)
HMEHFX_2078(aceB)
HGIABY42_09665 ABY42_16600
HALEG3A49_07690 G3A49_12900
HLSKU306_02605
HBOHbor_30530
HLMDV707_13970
SRUBC2R22_21540
HALGHUG10_03375
HALUHUG12_13540
HMPK6T50_01580
HREK6T36_01385
HRMK6T25_15035
HACBHbl1158_08495
HDFAArcSl_1031(aceB)
HTUHtur_4695
HSALJMJ58_24075
NMGNmag_3363(aceB)
NOUNatoc_4308
NAJB1756_06555
NBGDV706_17910
NASGCU68_18820
 » show all
Reference
1  [PMID:20047909]
  Authors
Erb TJ, Frerichs-Revermann L, Fuchs G, Alber BE
  Title
The apparent malate synthase activity of Rhodobacter sphaeroides is due to two paralogous enzymes, (3S)-Malyl-coenzyme A (CoA)/{beta}-methylmalyl-CoA lyase and (3S)- Malyl-CoA thioesterase.
  Journal
J Bacteriol 192:1249-58 (2010)
DOI:10.1128/JB.01267-09
  Sequence
[rsp:RSP_0970]
Other DBs
ExplorEnz - The Enzyme Database: 3.1.2.30
IUBMB Enzyme Nomenclature: 3.1.2.30
ExPASy - ENZYME nomenclature database: 3.1.2.30
BRENDA, the Enzyme Database: 3.1.2.30
LinkDB

KEGG   REACTION: R10612
Entry
R10612                      Reaction                               
Name
(S)-malyl-CoA hydrolase
Definition
L-Malyl-CoA + H2O <=> (S)-Malate + CoA
Equation
Reaction class
RC00004  C00010_C04348
RC00014  C00149_C04348
Enzyme
Pathway
rn00630  Glyoxylate and dicarboxylate metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00012  Glyoxylate cycle
M00373  Ethylmalonyl pathway
M00740  Methylaspartate cycle
Orthology
K14451  (3S)-malyl-CoA thioesterase [EC:3.1.2.30]
K19282  bifunctional (S)-malyl-CoA lyase/thioesterase [EC:4.1.3.24 3.1.2.30]
Other DBs
RHEA: 38294
LinkDB

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