KEGG   ORTHOLOGY: K14481
Entry
K14481            Tight     KO                                     
Symbol
styA
Name
styrene monooxygenase [EC:1.14.14.11]
Pathway
map00643  Styrene degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
Reaction
R05488  styrene,FADH2:oxygen oxidoreductase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00643 Styrene degradation
    K14481  styA; styrene monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.11  styrene monooxygenase
     K14481  styA; styrene monooxygenase
Other DBs
GO: 0018650
Genes
PSV: PVLB_26557
Reference
PMID:12906358
  Authors
Lorenzo P, Alonso S, Velasco A, Diaz E, Garcia JL, Perera J
  Title
Design of catabolic cassettes for styrene biodegradation.
  Journal
Antonie Van Leeuwenhoek 84:17-24 (2003)
DOI:10.1023/A:1024432929423
Reference
PMID:15292130
  Authors
Otto K, Hofstetter K, Rothlisberger M, Witholt B, Schmid A
  Title
Biochemical characterization of StyAB from Pseudomonas sp. strain VLB120 as a two-component flavin-diffusible monooxygenase.
  Journal
J Bacteriol 186:5292-302 (2004)
DOI:10.1128/JB.186.16.5292-5302.2004
  Sequence
[psv:PVLB_26557]
LinkDB

KEGG   ORTHOLOGY: K14482
Entry
K14482            Tight     KO                                     
Symbol
styB
Name
styrene monooxygenase reductase component [EC:1.5.1.-]
Pathway
map00643  Styrene degradation
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
Reaction
R05488  styrene,FADH2:oxygen oxidoreductase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00643 Styrene degradation
    K14482  styB; styrene monooxygenase reductase component
Other DBs
GO: 0018650
Genes
PSV: PVLB_26562
Reference
PMID:9172343
  Authors
Beltrametti F, Marconi AM, Bestetti G, Colombo C, Galli E, Ruzzi M, Zennaro E
  Title
Sequencing and functional analysis of styrene catabolism genes from Pseudomonas fluorescens ST.
  Journal
Appl Environ Microbiol 63:2232-9 (1997)
DOI:10.1128/AEM.63.6.2232-2239.1997
  Sequence
[psv:PVLB_26562]
Reference
PMID:12906358
  Authors
Lorenzo P, Alonso S, Velasco A, Diaz E, Garcia JL, Perera J
  Title
Design of catabolic cassettes for styrene biodegradation.
  Journal
Antonie Van Leeuwenhoek 84:17-24 (2003)
DOI:10.1023/A:1024432929423
LinkDB

KEGG   ENZYME: 1.14.14.11
Entry
EC 1.14.14.11               Enzyme                                 
Name
styrene monooxygenase;
StyA;
SMO;
NSMOA
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
styrene,FADH2:oxygen oxidoreductase
Reaction(IUBMB)
styrene + FADH2 + O2 = (S)-2-phenyloxirane + FAD + H2O [RN:R05488]
Reaction(KEGG)
R05488
Substrate
styrene [CPD:C07083];
FADH2 [CPD:C01352];
O2 [CPD:C00007]
Product
(S)-2-phenyloxirane [CPD:C20782];
FAD [CPD:C00016];
H2O [CPD:C00001]
Comment
The enzyme catalyses the first step in the aerobic styrene degradation pathway. It forms a two-component system with a reductase (StyB) that utilizes NADH to reduce flavin-adenine dinucleotide, which is then transferred to the oxygenase.
History
EC 1.14.14.11 created 2011
Pathway
ec00643  Styrene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K14481  styrene monooxygenase
Genes
PSV: PVLB_26557
Reference
1  [PMID:15292130]
  Authors
Otto K, Hofstetter K, Rothlisberger M, Witholt B, Schmid A
  Title
Biochemical characterization of StyAB from Pseudomonas sp. strain VLB120 as a two-component flavin-diffusible monooxygenase.
  Journal
J Bacteriol 186:5292-302 (2004)
DOI:10.1128/JB.186.16.5292-5302.2004
  Sequence
[psv:PVLB_26557]
Reference
2  [PMID:20675468]
  Authors
Tischler D, Kermer R, Groning JA, Kaschabek SR, van Berkel WJ, Schlomann M
  Title
StyA1 and StyA2B from Rhodococcus opacus 1CP: a multifunctional styrene monooxygenase system.
  Journal
J Bacteriol 192:5220-7 (2010)
DOI:10.1128/JB.00723-10
  Sequence
[ag:ACR43973 ACR43974]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.11
IUBMB Enzyme Nomenclature: 1.14.14.11
ExPASy - ENZYME nomenclature database: 1.14.14.11
BRENDA, the Enzyme Database: 1.14.14.11
LinkDB

KEGG   REACTION: R05488
Entry
R05488                      Reaction                               
Name
styrene,FADH2:oxygen oxidoreductase
Definition
Styrene + FADH2 + Oxygen <=> (S)-2-Phenyloxirane + FAD + H2O
Equation
C07083 + C01352 + C00007 <=> C20782 + C00016 + C00001
Reaction class
RC00126  C00016_C01352
RC03238  C07083_C20782
Enzyme
1.14.14.11
Pathway
rn00643  Styrene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.11
     R05488  Styrene + FADH2 + Oxygen <=> (S)-2-Phenyloxirane + FAD + H2O
Orthology
K14481  styrene monooxygenase [EC:1.14.14.11]
K14482  styrene monooxygenase reductase component [EC:1.5.1.-]
Other DBs
RHEA: 31730
LinkDB

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