KEGG   ORTHOLOGY: K15871
Entry
K15871            Tight     KO                                     
Symbol
baiF
Name
bile acid CoA-transferase [EC:2.8.3.25]
Pathway
map00121  Secondary bile acid biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09103 Lipid metabolism
   00121 Secondary bile acid biosynthesis
    K15871  baiF; bile acid CoA-transferase
Enzymes [BR:ko01000]
 2. Transferases
  2.8  Transferring sulfur-containing groups
   2.8.3  CoA-transferases
    2.8.3.25  bile acid CoA-transferase
     K15871  baiF; bile acid CoA-transferase
Other DBs
RN: R07295 R11539
COG: COG1804
Genes
AG: AAC45415(baiF) ACF20979(baiK) ACF20982(baiK) ACF20987(baiF)
Reference
  Authors
Ridlon JM, Kang DJ, Hylemon PB
  Title
Isolation and characterization of a bile acid inducible 7alpha-dehydroxylating operon in Clostridium hylemonae TN271.
  Journal
Anaerobe 16:137-46 (2010)
DOI:10.1016/j.anaerobe.2009.05.004
  Sequence
Reference
PMID:9869646
  Authors
Ye HQ, Mallonee DH, Wells JE, Bjorkhem I, Hylemon PB
  Title
The bile acid-inducible baiF gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A hydrolase.
  Journal
J Lipid Res 40:17-23 (1999)
  Sequence
Reference
  Authors
Ridlon JM, Hylemon PB
  Title
Identification and characterization of two bile acid coenzyme A transferases from Clostridium scindens, a bile acid 7alpha-dehydroxylating intestinal bacterium.
  Journal
J Lipid Res 53:66-76 (2012)
DOI:10.1194/jlr.M020313
  Sequence
LinkDB

KEGG   ENZYME: 2.8.3.25
Entry
EC 2.8.3.25                 Enzyme                                 
Name
bile acid CoA-transferase;
baiF (gene name);
baiK (gene name);
bile acid coenzyme A transferase
Class
Transferases;
Transferring sulfur-containing groups;
CoA-transferases
Sysname
lithocholoyl-CoA:cholate CoA-transferase
Reaction(IUBMB)
(1) lithocholoyl-CoA + cholate = lithocholate + choloyl-CoA [RN:R11539];
(2) deoxycholoyl-CoA + cholate = deoxycholate + choloyl-CoA [RN:R07295]
Reaction(KEGG)
R07295 R11539
Substrate
lithocholoyl-CoA [CPD:C21449];
cholate [CPD:C00695];
deoxycholoyl-CoA [CPD:C15560]
Product
lithocholate [CPD:C03990];
choloyl-CoA [CPD:C01794];
deoxycholate [CPD:C04483]
Comment
The enzyme, characterized from the gut bacterium Clostridium scindens, catalyses the last step in bile acid 7alpha-dehydroxylation, the removal of the CoA moiety from the products. By using a transferase rather than hydrolase, the bacteria conserve the thioester bond energy, saving ATP molecules. Clostridium scindens possesses two forms of the enzyme, encoded by the baiF and baiK genes. While the enzymes have a broad acceptor specificity and can use allocholate, ursodeoxycholate, and beta-muricholate, the donor specificity is more strict. BaiF acts on lithocholoyl-CoA and deoxycholoyl-CoA, and BaiK acts only on the latter.
History
EC 2.8.3.25 created 2005 as EC 3.1.2.26, transferred 2016 to EC 2.8.3.25
Pathway
ec00121  Secondary bile acid biosynthesis
Orthology
K15871  bile acid CoA-transferase
Reference
1  [PMID:22021638]
  Authors
Ridlon JM, Hylemon PB
  Title
Identification and characterization of two bile acid coenzyme A transferases from Clostridium scindens, a bile acid 7alpha-dehydroxylating intestinal bacterium.
  Journal
J Lipid Res 53:66-76 (2012)
DOI:10.1194/jlr.M020313
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.8.3.25
IUBMB Enzyme Nomenclature: 2.8.3.25
ExPASy - ENZYME nomenclature database: 2.8.3.25
BRENDA, the Enzyme Database: 2.8.3.25
LinkDB

DBGET integrated database retrieval system