KEGG   ORTHOLOGY: K15946
Entry
K15946            Tight     KO                                     
Symbol
aknS
Name
aklavinone 7-alpha-L-rhodosaminyltransferase [EC:2.4.1.326]
Pathway
map01057  Biosynthesis of type II polyketide products
map01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K15946  aknS; aklavinone 7-alpha-L-rhodosaminyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.326  aklavinone 7-L-rhodosaminyltransferase
     K15946  aknS; aklavinone 7-alpha-L-rhodosaminyltransferase
Other DBs
RN: R09331
CAZy: GT1
Genes
SGAL: CP966_28995
AG: AAF73455(aknS)
Reference
PMID:17685523
  Authors
Leimkuhler C, Fridman M, Lupoli T, Walker S, Walsh CT, Kahne D
  Title
Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A.
  Journal
J Am Chem Soc 129:10546-50 (2007)
DOI:10.1021/ja072909o
  Sequence
[ag:AAF73455]
LinkDB

KEGG   ORTHOLOGY: K15947
Entry
K15947            Tight     KO                                     
Symbol
aknT
Name
aklavinone 7-alpha-L-rhodosaminyltransferase auxiliary protein
Pathway
map01057  Biosynthesis of type II polyketide products
map01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K15947  aknT; aklavinone 7-alpha-L-rhodosaminyltransferase auxiliary protein
Other DBs
RN: R09331
Genes
AG: AAF73456(aknT)
Reference
PMID:17685523
  Authors
Leimkuhler C, Fridman M, Lupoli T, Walker S, Walsh CT, Kahne D
  Title
Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A.
  Journal
J Am Chem Soc 129:10546-50 (2007)
DOI:10.1021/ja072909o
  Sequence
[ag:AAF73456]
LinkDB

KEGG   ENZYME: 2.4.1.326
Entry
EC 2.4.1.326                Enzyme                                 
Name
aklavinone 7-L-rhodosaminyltransferase;
AknS/AknT;
aklavinone 7-beta-L-rhodosaminyltransferase;
dTDP-beta-L-rhodosamine:aklavinone 7-alpha-L-rhodosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
dTDP-beta-L-rhodosamine:aklavinone 7-alpha-L-rhodosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
dTDP-beta-L-rhodosamine + aklavinone = dTDP + aclacinomycin T [RN:R09331]
Reaction(KEGG)
R09331
Substrate
dTDP-beta-L-rhodosamine [CPD:C18643];
aklavinone [CPD:C12424]
Product
dTDP [CPD:C00363];
aclacinomycin T [CPD:C18634]
Comment
Isolated from the bacterium Streptomyces galilaeus. Forms a complex with its accessory protein AknT, and has very low activity in its absence. The enzyme can also use dTDP-2-deoxy-beta-L-fucose. Involved in the biosynthesis of other aclacinomycins.
History
EC 2.4.1.326 created 2014, modified 2015
Pathway
ec01057  Biosynthesis of type II polyketide products
ec01110  Biosynthesis of secondary metabolites
Orthology
K15946  aklavinone 7-alpha-L-rhodosaminyltransferase
Genes
SGAL: CP966_28995
Reference
1  [PMID:15911373]
  Authors
Lu W, Leimkuhler C, Gatto GJ Jr, Kruger RG, Oberthur M, Kahne D, Walsh CT
  Title
AknT is an activating protein for the glycosyltransferase AknS in L-aminodeoxysugar transfer to the aglycone of aclacinomycin A.
  Journal
Chem Biol 12:527-34 (2005)
DOI:10.1016/j.chembiol.2005.02.016
  Sequence
[ag:AAF73455 AAF73456]
Reference
2  [PMID:17685523]
  Authors
Leimkuhler C, Fridman M, Lupoli T, Walker S, Walsh CT, Kahne D
  Title
Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A.
  Journal
J Am Chem Soc 129:10546-50 (2007)
DOI:10.1021/ja072909o
  Sequence
[ag:AAF73455 AAF73456]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.326
IUBMB Enzyme Nomenclature: 2.4.1.326
ExPASy - ENZYME nomenclature database: 2.4.1.326
BRENDA, the Enzyme Database: 2.4.1.326
LinkDB

KEGG   REACTION: R09331
Entry
R09331                      Reaction                               
Name
dTDP-beta-L-rhodosamine:aklavinone 7-alpha-L-rhodosaminyltransferase
Definition
Aklavinone + dTDP-beta-L-rhodosamine <=> Aclacinomycin T + dTDP
Equation
C12424 + C18643 <=> C18634 + C00363
Reaction class
RC00005  C00363_C18643
RC00049  C12424_C18634
Enzyme
2.4.1.326
Pathway
rn01057  Biosynthesis of type II polyketide products
rn01110  Biosynthesis of secondary metabolites
Orthology
K15946  aklavinone 7-alpha-L-rhodosaminyltransferase [EC:2.4.1.326]
K15947  aklavinone 7-alpha-L-rhodosaminyltransferase auxiliary protein
Other DBs
RHEA: 41567
LinkDB

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