KEGG   ORTHOLOGY: K15989
Entry
K15989            Tight     KO                                     
Symbol
tylMII
Name
tylactone mycaminosyltransferase [EC:2.4.1.316]
Pathway
map00522  Biosynthesis of 12-, 14- and 16-membered macrolides
map01052  Type I polyketide structures
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00773  Tylosin biosynthesis, methylmalonyl-CoA + malonyl-CoA => tylactone => tylosin
Reaction
R06450  dTDP-alpha-D-mycaminose:tylactone 5-O-beta-D-mycaminosyltransferase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01052 Type I polyketide structures
    K15989  tylMII; tylactone mycaminosyltransferase
   00522 Biosynthesis of 12-, 14- and 16-membered macrolides
    K15989  tylMII; tylactone mycaminosyltransferase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K15989  tylMII; tylactone mycaminosyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.316  tylactone mycaminosyltransferase
     K15989  tylMII; tylactone mycaminosyltransferase
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide tailoring proteins
  Glycosyltransferase
   K15989  tylMII; tylactone mycaminosyltransferase
Other DBs
CAZy: GT1
Genes
AG: CAA57472(tylMII)
Reference
PMID:11476481
  Authors
Rawlings BJ.
  Title
Type I polyketide biosynthesis in bacteria (part B).
  Journal
Nat Prod Rep 18:231-81 (2001)
DOI:10.1039/B100191O
Reference
PMID:9031628
  Authors
Gandecha AR, Large SL, Cundliffe E
  Title
Analysis of four tylosin biosynthetic genes from the tylLM region of the Streptomyces fradiae genome.
  Journal
Gene 184:197-203 (1997)
DOI:10.1016/S0378-1119(96)00595-1
  Sequence
[ag:CAA57472]
Reference
PMID:15612702
  Authors
Melancon CE 3rd, Takahashi H, Liu HW
  Title
Characterization of tylM3/tylM2 and mydC/mycB pairs required for efficient glycosyltransfer in macrolide antibiotic biosynthesis.
  Journal
J Am Chem Soc 126:16726-7 (2004)
DOI:10.1021/ja043900e
  Sequence
[ag:CAA57472]
LinkDB

KEGG   ENZYME: 2.4.1.316
Entry
EC 2.4.1.316                Enzyme                                 
Name
tylactone mycaminosyltransferase;
tylM2 (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
dTDP-alpha-D-mycaminose:tylactone 5-O-beta-D-mycaminosyltransferase
Reaction(IUBMB)
tylactone + dTDP-alpha-D-mycaminose = dTDP + 5-O-beta-D-mycaminosyltylactone [RN:R06450]
Reaction(KEGG)
R06450
Substrate
tylactone [CPD:C12000];
dTDP-alpha-D-mycaminose [CPD:C11933]
Product
dTDP [CPD:C00363];
5-O-beta-D-mycaminosyltylactone [CPD:C12001]
Comment
The enzyme participates in the biosynthetic pathway of the macrolide antibiotic tylosin, which is produced by several species of Streptomyces bacteria. Activity is significantly enhanced by the presence of an accessory protein encoded by the tylM3 gene.
History
EC 2.4.1.316 created 2014
Pathway
ec00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K15989  tylactone mycaminosyltransferase
Reference
1  [PMID:9031628]
  Authors
Gandecha AR, Large SL, Cundliffe E
  Title
Analysis of four tylosin biosynthetic genes from the tylLM region of the Streptomyces fradiae genome.
  Journal
Gene 184:197-203 (1997)
DOI:10.1016/S0378-1119(96)00595-1
  Sequence
[ag:CAA57472]
Reference
2  [PMID:15612702]
  Authors
Melancon CE 3rd, Takahashi H, Liu HW
  Title
Characterization of tylM3/tylM2 and mydC/mycB pairs required for efficient glycosyltransfer in macrolide antibiotic biosynthesis.
  Journal
J Am Chem Soc 126:16726-7 (2004)
DOI:10.1021/ja043900e
  Sequence
[ag:CAA57472]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.316
IUBMB Enzyme Nomenclature: 2.4.1.316
ExPASy - ENZYME nomenclature database: 2.4.1.316
BRENDA, the Enzyme Database: 2.4.1.316
LinkDB

KEGG   REACTION: R06450
Entry
R06450                      Reaction                               
Name
dTDP-alpha-D-mycaminose:tylactone 5-O-beta-D-mycaminosyltransferase
Definition
Tylactone + dTDP-D-mycaminose <=> 5-O-beta-D-Mycaminosyltylactone + dTDP
Equation
C12000 + C11933 <=> C12001 + C00363
Reaction class
RC00005  C00363_C11933
RC00049  C12000_C12001
Enzyme
2.4.1.316
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00773  Tylosin biosynthesis, methylmalonyl-CoA + malonyl-CoA => tylactone => tylosin
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.316
     R06450  Tylactone + dTDP-D-mycaminose <=> 5-O-beta-D-Mycaminosyltylactone + dTDP
Orthology
K15989  tylactone mycaminosyltransferase [EC:2.4.1.316]
K15990  tylactone mycaminosyltransferase auxiliary protein
Other DBs
RHEA: 21471
LinkDB

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