KEGG   ORTHOLOGY: K15995
Entry
K15995            Tight     KO                                     
Symbol
tylE
Name
demethylmacrocin O-methyltransferase [EC:2.1.1.102]
Pathway
map00522  Biosynthesis of 12-, 14- and 16-membered macrolides
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00773  Tylosin biosynthesis, methylmalonyl-CoA + malonyl-CoA => tylactone => tylosin
Reaction
R02859  S-adenosyl-L-methionine:demethylmacrocin 2'''-O-methyltransferase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00522 Biosynthesis of 12-, 14- and 16-membered macrolides
    K15995  tylE; demethylmacrocin O-methyltransferase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K15995  tylE; demethylmacrocin O-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.102  demethylmacrocin O-methyltransferase
     K15995  tylE; demethylmacrocin O-methyltransferase
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide tailoring proteins
  Methylase
   K15995  tylE; demethylmacrocin O-methyltransferase
Other DBs
GO: 0030770
Genes
AG: AAD12164(tylE)
Reference
PMID:10220165
  Authors
Fouces R, Mellado E, Diez B, Barredo JL
  Title
The tylosin biosynthetic cluster from Streptomyces fradiae: genetic organization of the left region.
  Journal
Microbiology 145 ( Pt 4):855-68 (1999)
DOI:10.1099/13500872-145-4-855
Reference
PMID:3170602
  Authors
Kreuzman AJ, Turner JR, Yeh WK
  Title
Two distinctive O-methyltransferases catalyzing penultimate and terminal reactions of macrolide antibiotic (tylosin) biosynthesis. Substrate specificity, enzyme inhibition, and kinetic mechanism.
  Journal
J Biol Chem 263:15626-33 (1988)
  Sequence
[ag:AAD12164]
LinkDB

KEGG   ENZYME: 2.1.1.102
Entry
EC 2.1.1.102                Enzyme                                 
Name
demethylmacrocin O-methyltransferase;
demethylmacrocin methyltransferase;
DMOMT
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:demethylmacrocin 2'''-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + demethylmacrocin = S-adenosyl-L-homocysteine + macrocin [RN:R02859]
Reaction(KEGG)
R02859
Substrate
S-adenosyl-L-methionine [CPD:C00019];
demethylmacrocin [CPD:C02400]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
macrocin [CPD:C00744]
Comment
Requires Mg2+. The enzyme, isolated from the bacterium Streptomyces fradiae, is involved in the biosynthesis of the macrolide antibiotic tylosin. The 2-hydroxy group of a 6-deoxy-D-allose moiety in demethylmacrocin acts as the methyl acceptor. Also acts on demethyllactenocin, giving lactenocin. Not identical with EC 2.1.1.101 macrocin O-methyltransferase.
History
EC 2.1.1.102 created 1992
Pathway
ec00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K15995  demethylmacrocin O-methyltransferase
Reference
1  [PMID:3170602]
  Authors
Kreuzman AJ, Turner JR, Yeh WK
  Title
Two distinctive O-methyltransferases catalyzing penultimate and terminal reactions of macrolide antibiotic (tylosin) biosynthesis. Substrate specificity, enzyme inhibition, and kinetic mechanism.
  Journal
J Biol Chem 263:15626-33 (1988)
  Sequence
[ag:AAD12164]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.102
IUBMB Enzyme Nomenclature: 2.1.1.102
ExPASy - ENZYME nomenclature database: 2.1.1.102
BRENDA, the Enzyme Database: 2.1.1.102
CAS: 120313-64-6
LinkDB

KEGG   REACTION: R02859
Entry
R02859                      Reaction                               
Name
S-adenosyl-L-methionine:demethylmacrocin 2'''-O-methyltransferase
Definition
S-Adenosyl-L-methionine + Demethylmacrocin <=> S-Adenosyl-L-homocysteine + Macrocin
Equation
C00019 + C02400 <=> C00021 + C00744
Comment
demethylmacrocin-O-methyltransferase,TylE
Reaction class
RC00003  C00019_C00021
RC00466  C00744_C02400
Enzyme
2.1.1.102
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00773  Tylosin biosynthesis, methylmalonyl-CoA + malonyl-CoA => tylactone => tylosin
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.102
     R02859  S-Adenosyl-L-methionine + Demethylmacrocin <=> S-Adenosyl-L-homocysteine + Macrocin
Orthology
K15995  demethylmacrocin O-methyltransferase [EC:2.1.1.102]
Other DBs
RHEA: 17576
LinkDB

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