KEGG   ORTHOLOGY: K15997
Entry
K15997                      KO                                     
Symbol
eryCII
Name
3-alpha-mycarosylerythronolide B desosaminyl transferase auxiliary protein
Pathway
map00522  Biosynthesis of 12-, 14- and 16-membered macrolides
map01052  Type I polyketide structures
map01100  Metabolic pathways
map01110  Biosynthesis of secondary metabolites
Module
M00774  Erythromycin biosynthesis, propanoyl-CoA + methylmalonyl-CoA => deoxyerythronolide B => erythromycin A/B
Reaction
R05531  dTDP-D-desosamine:3-O-mycarosylerythronolide-B 5-O-beta-D-desosaminyltransferase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01052 Type I polyketide structures
    K15997  eryCII; 3-alpha-mycarosylerythronolide B desosaminyl transferase auxiliary protein
   00522 Biosynthesis of 12-, 14- and 16-membered macrolides
    K15997  eryCII; 3-alpha-mycarosylerythronolide B desosaminyl transferase auxiliary protein
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K15997  eryCII; 3-alpha-mycarosylerythronolide B desosaminyl transferase auxiliary protein
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide tailoring proteins
  Glycosyltransferase
   K15997  eryCII; 3-alpha-mycarosylerythronolide B desosaminyl transferase auxiliary protein
Genes
SNR: SNOUR_10100(eryCII)
SGF: HEP81_07909(eryCII)
SEN: SACE_0725(eryCII)
AEY: CDG81_13165
AG: AAG13920(megCII)
Reference
PMID:22056329
  Authors
Moncrieffe MC, Fernandez MJ, Spiteller D, Matsumura H, Gay NJ, Luisi BF, Leadlay PF
  Title
Structure of the glycosyltransferase EryCIII in complex with its activating P450 homologue EryCII.
  Journal
J Mol Biol 415:92-101 (2012)
DOI:10.1016/j.jmb.2011.10.036
Reference
PMID:16218575
  Authors
Yuan Y, Chung HS, Leimkuhler C, Walsh CT, Kahne D, Walker S
  Title
In vitro reconstitution of EryCIII activity for the preparation of unnatural macrolides.
  Journal
J Am Chem Soc 127:14128-9 (2005)
DOI:10.1021/ja053704n
Reference
PMID:16514147
  Authors
Rodriguez E, Peiru S, Carney JR, Gramajo H
  Title
In vivo characterization of the dTDP-D-desosamine pathway of the megalomicin gene cluster from Micromonospora megalomicea.
  Journal
Microbiology 152:667-73 (2006)
DOI:10.1099/mic.0.28680-0
LinkDB

KEGG   REACTION: R05531
Entry
R05531                      Reaction                               
Name
dTDP-D-desosamine:3-O-mycarosylerythronolide-B 5-O-beta-D-desosaminyltransferase
Definition
dTDP-D-desosamine + 3-O-alpha-Mycarosylerythronolide B <=> Erythromycin D + dTDP
Equation
C11911 + C06630 <=> C06633 + C00363
Comment
desosaminyltransferase, EryCIII+EryCII, MegCIII+MegCII
Reaction class
RC00005  C00363_C11911
RC00049  C06630_C06633
Enzyme
2.4.1.278
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00774  Erythromycin biosynthesis, propanoyl-CoA + methylmalonyl-CoA => deoxyerythronolide B => erythromycin A/B
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.278
     R05531  dTDP-D-desosamine + 3-O-alpha-Mycarosylerythronolide B <=> Erythromycin D + dTDP
Orthology
K14368  3-alpha-mycarosylerythronolide B desosaminyl transferase [EC:2.4.1.278]
K15997  3-alpha-mycarosylerythronolide B desosaminyl transferase auxiliary protein
Reference
1  [PMID:9353926]
  Authors
Summers RG, Donadio S, Staver MJ, Wendt-Pienkowski E, Hutchinson CR, Katz L.
  Title
Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production.
  Journal
Microbiology 143:3251-62 (1997)
DOI:10.1099/00221287-143-10-3251
Reference
2  [PMID:22056329]
  Authors
Moncrieffe MC, Fernandez MJ, Spiteller D, Matsumura H, Gay NJ, Luisi BF, Leadlay PF
  Title
Structure of the glycosyltransferase EryCIII in complex with its activating P450 homologue EryCII.
  Journal
J Mol Biol 415:92-101 (2012)
DOI:10.1016/j.jmb.2011.10.036
Reference
3  [PMID:16514147]
  Authors
Rodriguez E, Peiru S, Carney JR, Gramajo H
  Title
In vivo characterization of the dTDP-D-desosamine pathway of the megalomicin gene cluster from Micromonospora megalomicea.
  Journal
Microbiology 152:667-73 (2006)
DOI:10.1099/mic.0.28680-0
Other DBs
RHEA: 32094
LinkDB

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