KEGG   ORTHOLOGY: K16051
Entry
K16051                      KO                                     
Symbol
tesI
Name
3-oxo-5alpha-steroid 4-dehydrogenase [EC:1.3.99.5]
Pathway
map00984  Steroid degradation
map01120  Microbial metabolism in diverse environments
Reaction
R01834  3-oxo-5alpha-steroid:(acceptor) delta4-oxidoreductase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00984 Steroid degradation
    K16051  tesI; 3-oxo-5alpha-steroid 4-dehydrogenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.3  Acting on the CH-CH group of donors
   1.3.99  With unknown physiological acceptors
    1.3.99.5  3-oxo-5alpha-steroid 4-dehydrogenase (acceptor)
     K16051  tesI; 3-oxo-5alpha-steroid 4-dehydrogenase
Other DBs
COG: COG1053
GO: 0003865
Genes
PRE: PCA10_43670
PFUW: KF707C_11860
PPUT: L483_14100
PPUN: PP4_27190
PPUU: PputUW4_01680
PKC: PKB_4321
PFZ: AV641_10570
PLQ: AA042_20375
PPSY: AOC04_07870
PSET: THL1_4409
PSIL: PMA3_11575
PKE: DLD99_14665
PUM: HGP31_20980
PPSH: G5J76_13525
PLAL: FXN65_21135
PAZE: KSS91_19195
PTAE: NCTC10697_02064(ifcA_1)
PCAV: D3880_17460
PIZ: LAB08_R40770
PKM: PZ739_11580
PSHS: JJN09_05510
PFOU: OZ911_14115
PSKP: KU43P_19180
KAK: Kalk_07950
CBER: B5D82_11210
MSEC: LN244_04835
MRZ: KDW95_00830
ENW: MJO57_21290
EMP: EZMO1_1275(tesI)
SOK: D0B54_05270
SINI: GT972_08465
FCE: JN531_005455
GPB: HDN1F_19870(tesI)
REH: H16_B0641(h16_B0641)
CNC: CNE_2c05860
CUH: BJN34_30935
CTI: RALTA_B0490
CBW: RR42_s2342
COX: E0W60_08150
CUK: KB879_20645
BFN: OI25_4613
PBRY: NDK50_33000
PPAI: E1956_26865 E1956_37100
CABA: SBC2_48770
CTT: CtCNB1_1358
CTES: O987_07765
CRJ: QMY55_07045
DIH: G7047_09730
SMIO: CATMQ487_31560
DOE: DENOEST_3316
TSV: DSM104635_00611
NAR: Saro_3721
NPP: PP1Y_Mpl3259
SWI: Swit_1005 Swit_1860 Swit_3335
SPHD: HY78_08875 HY78_16270 HY78_21490
RDI: CMV14_19635
SPHM: G432_07425
SSAN: NX02_12730
SPKC: KC8_10970
SPHA: D3Y57_03785 D3Y57_15195
SPHB: EP837_01509(tesI)
SPHG: AZE99_00535
NTD: EGO55_03145 EGO55_13615
CAEN: K5X80_05260 K5X80_13780
MUL: MUL_4900
MMI: MMAR_0205
MMAE: MMARE11_01890
MLI: MULP_00179
MPSE: MPSD_53970
MSHO: MSHO_12420
MMC: Mmcs_0302
MKM: Mkms_0312
MJL: Mjls_0293
MKN: MKAN_15315
MYV: G155_02520
MFJ: MFLOJ_21820
MSTO: MSTO_25520
MKU: I2456_01305 I2456_01760
MPAG: C0J29_01190
MNM: MNVM_40340
MGOR: H0P51_01160
MBAI: MB901379_00137
MLJ: MLAC_19640
MSHJ: MSHI_01900
MSPG: F6B93_00910
MOT: LTS72_24685
MKY: IWGMT90018_02350
MSM: MSMEG_2873
MSG: MSMEI_2799
MSB: LJ00_14290
MSN: LI99_14295
MSH: LI98_14300
MGI: Mflv_4564
MSP: Mspyr1_39640
MFT: XA26_04570
MPHL: MPHLCCUG_02608
MVQ: MYVA_1640 MYVA_5433
MTHN: 4412656_00946(ifcA_1)
MHAS: MHAS_02476
MCHT: MCHIJ_12690
MFX: MFAL_17780
MIJ: MINS_21440
MALV: MALV_32080
MAUB: MAUB_04580
MPHU: MPHO_08050
MMUC: C1S78_014220
MBOK: MBOE_16350
MFLV: NCTC10271_02169(ifcA_1)
MMON: EWR22_01615
MDF: K0O62_10315 K0O62_15920
MKR: MKOR_07390
MPAK: MIU77_08035
MAB: MAB_0623
MMV: MYCMA_0323
MABB: MASS_0593
MABL: MMASJCM_0612
MCHE: BB28_02985
MIZ: BAB75_03735
MSTE: MSTE_00582
MSAO: MYCSP_02685
MSAL: DSM43276_00541
MJD: JDM601_2019
MTER: 4434518_01971
MMIN: MMIN_36500
MHIB: MHIB_10920 MHIB_14890
MHER: K3U94_11545
MVM: MJO54_11545
NFA: NFA_4640
NFR: ERS450000_03773(ifcA_3)
NCY: NOCYR_0491
NBR: O3I_002615
NSL: BOX37_17180
NOD: FOH10_02275
NAH: F5544_02605
NAD: NCTC11293_04109(ifcA_2)
NIE: KV110_02995
NTC: KHQ06_36955
NHU: H0264_36175
NYA: LTV02_37155
NGP: LTT66_15370
NSPU: IFM12276_01570
RER: RER_07380
REY: O5Y_03585
REB: XU06_03825
RQI: C1M55_04070
RHOD: AOT96_14465
RTM: G4H71_07430
RGO: KYT97_16405 KYT97_23105
RHOP: D8W71_26930
RHOO: RA302_03945
REQ: REQ_08850
TSM: ASU32_08060
TPUL: TPB0596_44260
TSD: MTP03_16260
CBQ: AL705_03940
AARC: G127AT_04070
AJG: KKR91_06375
ACAO: NF551_01370
FAL: FRAAL3038
AMD: AMED_2521
AMN: RAM_12815
AMM: AMES_2493
AMZ: B737_2494
AOI: AORI_2483
AJA: AJAP_26410
AMQ: AMETH_4839
AMYC: CU254_10455
AMYB: BKN51_05015
AAB: A4R43_16050
AMYY: YIM_14445
AORI: SD37_13480
AACD: LWP59_23765 LWP59_29205
APRT: MUY14_23835
AROO: NQK81_09935
ATHM: L1857_26970
ARHD: VSH64_01775
AMOG: QRX60_26335
ACOR: LCL61_01240
PSDS: K1T35_06290
KAL: KALB_5139
KUT: JJ691_07860
KPHY: AOZ06_42835
LED: BBK82_38945
ALO: CRK59602
PMAD: BAY61_10950
SACE: GIY23_13360
CROS: N8J89_29395
STP: Strop_2675
SAQ: Sare_2883
AMS: AMIS_19130
AIH: Aiant_18970
ACTL: L3i22_084550
ASIC: Q0Z83_065660
AOU: ACTOB_004112
ACTE: ACTI_41160
AG: AAB08517(tesI) BAC81693(tesI)
 » show all
Reference
PMID:8655514
  Authors
Florin C, Kohler T, Grandguillot M, Plesiat P
  Title
Comamonas testosteroni 3-ketosteroid-delta 4(5 alpha)-dehydrogenase: gene and protein characterization.
  Journal
J Bacteriol 178:3322-30 (1996)
DOI:10.1128/JB.178.11.3322-3330.1996
  Sequence
[ag:AAB08517]
Reference
PMID:12902225
  Authors
Horinouchi M, Hayashi T, Yamamoto T, Kudo T
  Title
A new bacterial steroid degradation gene cluster in Comamonas testosteroni TA441 which consists of aromatic-compound degradation genes for seco-steroids and 3-ketosteroid dehydrogenase genes.
  Journal
Appl Environ Microbiol 69:4421-30 (2003)
DOI:10.1128/AEM.69.8.4421-4430.2003
  Sequence
[ag:BAC81693]
Reference
PMID:20554032
  Authors
Horinouchi M, Kurita T, Hayashi T, Kudo T
  Title
Steroid degradation genes in Comamonas testosteroni TA441: Isolation of genes encoding a Delta4(5)-isomerase and 3alpha- and 3beta-dehydrogenases and evidence for a 100 kb steroid degradation gene hot spot.
  Journal
J Steroid Biochem Mol Biol 122:253-63 (2010)
DOI:10.1016/j.jsbmb.2010.06.002
LinkDB

KEGG   ENZYME: 1.3.99.5
Entry
EC 1.3.99.5                 Enzyme                                 
Name
3-oxo-5alpha-steroid 4-dehydrogenase (acceptor);
steroid 5alpha-reductase;
3-oxosteroid Delta4-dehydrogenase;
3-oxo-5alpha-steroid Delta4-dehydrogenase;
steroid Delta4-5alpha-reductase;
Delta4-3-keto steroid 5alpha-reductase;
Delta4-3-oxo steroid reductase;
Delta4-3-ketosteroid5alpha-oxidoreductase;
Delta4-3-oxosteroid-5alpha-reductase;
3-keto-Delta4-steroid-5alpha-reductase;
5alpha-reductase;
testosterone 5alpha-reductase;
4-ene-3-ketosteroid-5alpha-oxidoreductase;
Delta4-5alpha-dehydrogenase;
3-oxo-5alpha-steroid:(acceptor) Delta4-oxidoreductase;
tesI (gene name)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors
Sysname
3-oxo-5alpha-steroid:acceptor Delta4-oxidoreductase
Reaction(IUBMB)
a 3-oxo-5alpha-steroid + acceptor = a 3-oxo-Delta4-steroid + reduced acceptor [RN:R02643]
Reaction(KEGG)
R02643 > R01834 R04827
Substrate
3-oxo-5alpha-steroid [CPD:C02940];
acceptor [CPD:C00028]
Product
3-oxo-Delta4-steroid [CPD:C00619];
reduced acceptor [CPD:C00030]
Comment
A flavoprotein. This bacterial enzyme, characterized from Comamonas testosteroni, is involved in androsterone degradation. cf. EC 1.3.1.22, 3-oxo-5alpha-steroid 4-dehydrogenase (NADP+).
History
EC 1.3.99.5 created 1965, modified 2012
Pathway
ec00984  Steroid degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K16051  3-oxo-5alpha-steroid 4-dehydrogenase
Genes
PRE: PCA10_43670
PFUW: KF707C_11860
PPUT: L483_14100
PPUN: PP4_27190
PPUU: PputUW4_01680
PKC: PKB_4321
PFZ: AV641_10570
PLQ: AA042_20375
PPSY: AOC04_07870
PSET: THL1_4409
PSIL: PMA3_11575
PKE: DLD99_14665
PUM: HGP31_20980
PPSH: G5J76_13525
PLAL: FXN65_21135
PAZE: KSS91_19195
PTAE: NCTC10697_02064(ifcA_1)
PCAV: D3880_17460
PIZ: LAB08_R40770
PKM: PZ739_11580
PSHS: JJN09_05510
PFOU: OZ911_14115
PSKP: KU43P_19180
KAK: Kalk_07950
CBER: B5D82_11210
MSEC: LN244_04835
MRZ: KDW95_00830
ENW: MJO57_21290
EMP: EZMO1_1275(tesI)
SOK: D0B54_05270
SINI: GT972_08465
FCE: JN531_005455
GPB: HDN1F_19870(tesI)
REH: H16_B0641(h16_B0641)
CNC: CNE_2c05860
CUH: BJN34_30935
CTI: RALTA_B0490
CBW: RR42_s2342
COX: E0W60_08150
CUK: KB879_20645
BFN: OI25_4613
PBRY: NDK50_33000
PPAI: E1956_26865 E1956_37100
CABA: SBC2_48770
CTT: CtCNB1_1358
CTES: O987_07765
CRJ: QMY55_07045
DIH: G7047_09730
SMIO: CATMQ487_31560
DOE: DENOEST_3316
TSV: DSM104635_00611
NAR: Saro_3721
NPP: PP1Y_Mpl3259
SWI: Swit_1005 Swit_1860 Swit_3335
SPHD: HY78_08875 HY78_16270 HY78_21490
RDI: CMV14_19635
SPHM: G432_07425
SSAN: NX02_12730
SPKC: KC8_10970
SPHA: D3Y57_03785 D3Y57_15195
SPHB: EP837_01509(tesI)
SPHG: AZE99_00535
NTD: EGO55_03145 EGO55_13615
CAEN: K5X80_05260 K5X80_13780
MUL: MUL_4900
MMI: MMAR_0205
MMAE: MMARE11_01890
MLI: MULP_00179
MPSE: MPSD_53970
MSHO: MSHO_12420
MMC: Mmcs_0302
MKM: Mkms_0312
MJL: Mjls_0293
MKN: MKAN_15315
MYV: G155_02520
MFJ: MFLOJ_21820
MSTO: MSTO_25520
MKU: I2456_01305 I2456_01760
MPAG: C0J29_01190
MNM: MNVM_40340
MGOR: H0P51_01160
MBAI: MB901379_00137
MLJ: MLAC_19640
MSHJ: MSHI_01900
MSPG: F6B93_00910
MOT: LTS72_24685
MKY: IWGMT90018_02350
MSM: MSMEG_2873
MSG: MSMEI_2799
MSB: LJ00_14290
MSN: LI99_14295
MSH: LI98_14300
MGI: Mflv_4564
MSP: Mspyr1_39640
MFT: XA26_04570
MPHL: MPHLCCUG_02608
MVQ: MYVA_1640 MYVA_5433
MTHN: 4412656_00946(ifcA_1)
MHAS: MHAS_02476
MCHT: MCHIJ_12690
MFX: MFAL_17780
MIJ: MINS_21440
MALV: MALV_32080
MAUB: MAUB_04580
MPHU: MPHO_08050
MMUC: C1S78_014220
MBOK: MBOE_16350
MFLV: NCTC10271_02169(ifcA_1)
MMON: EWR22_01615
MDF: K0O62_10315 K0O62_15920
MKR: MKOR_07390
MPAK: MIU77_08035
MAB: MAB_0623
MMV: MYCMA_0323
MABB: MASS_0593
MABL: MMASJCM_0612
MCHE: BB28_02985
MIZ: BAB75_03735
MSTE: MSTE_00582
MSAO: MYCSP_02685
MSAL: DSM43276_00541
MJD: JDM601_2019
MTER: 4434518_01971
MMIN: MMIN_36500
MHIB: MHIB_10920 MHIB_14890
MHER: K3U94_11545
MVM: MJO54_11545
NFA: NFA_4640
NFR: ERS450000_03773(ifcA_3)
NCY: NOCYR_0491
NBR: O3I_002615
NSL: BOX37_17180
NOD: FOH10_02275
NAH: F5544_02605
NAD: NCTC11293_04109(ifcA_2)
NIE: KV110_02995
NTC: KHQ06_36955
NHU: H0264_36175
NYA: LTV02_37155
NGP: LTT66_15370
NSPU: IFM12276_01570
RER: RER_07380
REY: O5Y_03585
REB: XU06_03825
RQI: C1M55_04070
RHOD: AOT96_14465
RTM: G4H71_07430
RGO: KYT97_16405 KYT97_23105
RHOP: D8W71_26930
RHOO: RA302_03945
REQ: REQ_08850
TSM: ASU32_08060
TPUL: TPB0596_44260
TSD: MTP03_16260
CBQ: AL705_03940
AARC: G127AT_04070
AJG: KKR91_06375
ACAO: NF551_01370
FAL: FRAAL3038
AMD: AMED_2521
AMN: RAM_12815
AMM: AMES_2493
AMZ: B737_2494
AOI: AORI_2483
AJA: AJAP_26410
AMQ: AMETH_4839
AMYC: CU254_10455
AMYB: BKN51_05015
AAB: A4R43_16050
AMYY: YIM_14445
AORI: SD37_13480
AACD: LWP59_23765 LWP59_29205
APRT: MUY14_23835
AROO: NQK81_09935
ATHM: L1857_26970
ARHD: VSH64_01775
AMOG: QRX60_26335
ACOR: LCL61_01240
PSDS: K1T35_06290
KAL: KALB_5139
KUT: JJ691_07860
KPHY: AOZ06_42835
LED: BBK82_38945
ALO: CRK59602
PMAD: BAY61_10950
SACE: GIY23_13360
CROS: N8J89_29395
STP: Strop_2675
SAQ: Sare_2883
AMS: AMIS_19130
AIH: Aiant_18970
ACTL: L3i22_084550
ASIC: Q0Z83_065660
AOU: ACTOB_004112
ACTE: ACTI_41160
 » show all
Reference
1  [PMID:13673006]
  Authors
LEVY HR, TALALAY P.
  Title
Bacterial oxidation of steroids. II. Studies on the enzymatic mechanism of ring A dehydrogenation.
  Journal
J Biol Chem 234:2014-21 (1959)
Reference
2  [PMID:8655514]
  Authors
Florin C, Kohler T, Grandguillot M, Plesiat P
  Title
Comamonas testosteroni 3-ketosteroid-delta 4(5 alpha)-dehydrogenase: gene and protein characterization.
  Journal
J Bacteriol 178:3322-30 (1996)
DOI:10.1128/JB.178.11.3322-3330.1996
  Sequence
[ag:AAB08517]
Reference
3  [PMID:12902225]
  Authors
Horinouchi M, Hayashi T, Yamamoto T, Kudo T
  Title
A new bacterial steroid degradation gene cluster in Comamonas testosteroni TA441 which consists of aromatic-compound degradation genes for seco-steroids and 3-ketosteroid dehydrogenase genes.
  Journal
Appl Environ Microbiol 69:4421-30 (2003)
DOI:10.1128/AEM.69.8.4421-4430.2003
  Sequence
[ag:BAC81693]
Other DBs
ExplorEnz - The Enzyme Database: 1.3.99.5
IUBMB Enzyme Nomenclature: 1.3.99.5
ExPASy - ENZYME nomenclature database: 1.3.99.5
BRENDA, the Enzyme Database: 1.3.99.5
CAS: 9036-43-5
LinkDB

KEGG   REACTION: R01834
Entry
R01834                      Reaction                               
Name
3-oxo-5alpha-steroid:(acceptor) delta4-oxidoreductase
Definition
5alpha-Androstane-3,17-dione + Acceptor <=> Androstenedione + Reduced acceptor
Equation
C00674 + C00028 <=> C00280 + C00030
Reaction class
RC00145  C00280_C00674
Enzyme
1.3.99.5
Pathway
rn00984  Steroid degradation
rn01120  Microbial metabolism in diverse environments
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.3  Acting on the CH-CH group of donors
   1.3.99  With unknown physiological acceptors
    1.3.99.5
     R01834  5alpha-Androstane-3,17-dione + Acceptor <=> Androstenedione + Reduced acceptor
IUBMB reaction hierarchy [BR:br08202]
 1. Oxidoreductase reactions
  1.3.99.5
   R02643  3-Oxo-5alpha-steroid <=> 3-Oxo-Delta4-steroid
    R01834  5alpha-Androstane-3,17-dione <=> Androstenedione
Orthology
K16051  3-oxo-5alpha-steroid 4-dehydrogenase [EC:1.3.99.5]
Other DBs
RHEA: 51051
LinkDB

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